• Title, Summary, Keyword: Staphylococcus aureus

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Assessment of Baried-Parker Agar as Screening Test for Determination of Staphylococcus aureus in Poultry Meat

  • Rosa Capita;Calleja, Carlos-Alnoso;Benito Moreno
    • Journal of Microbiology
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    • v.39 no.4
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    • pp.321-325
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    • 2001
  • Baird-Parker agar with egg yolk/tellurite emulsion (BPA) is widely accepted as a medium for the enu-meration of Staphylococcus aureus in foods. Howerver, it is not vompletely selective and colonies of other genera of species could be similar to those of Staphylococcus aureus. Moreover, the strains of Staphylococcus aureus that are lecithinase negatrive could go unnoticed. Both facts could affect the counts. The aim of this study was to dtermine whether the enumeration of the colonies with the typical morphol-ogy of Staphylococcus aureus on BPA is sufficien to quantity this species in poultry meat. Forty chiken carcasses were tested for Staphylococcus aureus by surface plating using BPA, Results indicate that the predictiv value of the morphoogy of the colonies of BPA is 85.71% and 68.42% for typical and atypical colonies of Staphylococcus aureus, respectively. However, Staphylococcus aureus counts (after identification) and counts of typical colonies did not show any significant differences(P>0.05) and are significantly(P<0.001) correlated (r=0.996).These results suggest that , for screening purposes. enumeration of Staphylococcus aureus from poultry meat does not require any identification of stains. resulting in a saving of time and money.

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Phage typing of Staphylococcus aureus Isolates from Poultry Meat in Spain

  • Rosa Capita;Astorga, Maite-Alvarez;Calleja, Carlos-Alonso;Benito Moreno
    • Journal of Microbiology
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    • v.39 no.3
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    • pp.219-225
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    • 2001
  • Phage typing is currently used for typing of Staphylococcus aureus strains beyond the species level in epidemiological studies. A total of 168 Staphylococcus aureus isolates from chicken meat and chicken by-products were phage-typed using the international bacteriophage set for typing Staphylococcus aureus of human origin. One hundred and forty-eight (88.79%) strains were phage-typeable (at least one phage produced 20 or more plaques of lysis). Lysis by phages of group Ⅲ was the mast frequent with 99 (58.93%) sensitive strains. This fact coincides with results of other authors. Twenty-nine different phage patterns were observed and three (95, 75/84 and 6/1030/W57) were most common. One hundred and thirty-two (89.19% of typeable strains) skewed these or indistinguishable (only one phage reaction difference) patterns. Twenty-six out of seventy chicken samples (37.14%) harboured more than one phage type of Staphylococcus aureus. This fact emphasizes the convenience of subtyping several Staphylococcus aureus isolates from the same sample in epidemiological studies. 80% of sausages and hamburgers contained the same Staphylococcus aureus phage types, which wore not found in any of the other food types. This fact suggests a cross contamination during the processing of these foods. Phages 6, 75, 84, 1030 and W57 skewed the greatest activity. None of the Staphylococcus aureus strains were sensitive to phages 47, 81 and 94.

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The Experimental Study on Anti-bacterial Potency of Jinpi-san on Staphylococcus aureus & Staphylococcus epidermidis. (진피산(秦皮散)이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.20 no.1
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    • pp.195-200
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    • 2007
  • Objective : This experimental study was performed to investigate the effect of Jinpi-san on Staphylococcus aureus(S. aureus) and Staphylococcus epidermidis(S. epidermidis) that induce keratitis. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ according to density Jinpi-san(100%, 10%, 1%, 0.1 %). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on S. aureus in Jinpi-san was $40{\mu}l$ undiluted solution. 2. MIC on S. epidermidis in Jinpi-san was $20{\mu}l$ undiluted solution. Conclusions : These results indicate that Jinpi-san can be used to cure S. aureus, S. epidermidis that induce eye disease(keratitis). If further study is performed, the use of eye drops will be valuable and beneficial in the clinical medicines.

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Amplified Fragment Length Polymorphism Fingerprinting as a Tool to Study the Genetic Diversity of Staphylococcus aureus Isolated from Food Sources

  • Kim, Young-Sam;Kim, Jong-Bae
    • Biomedical Science Letters
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    • v.8 no.1
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    • pp.39-46
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    • 2002
  • Amplified fragment length polymorphism (AFLP) is a recently developed PCR-based high resolution fingerprinting method that is able to generate complex banding patterns which can be used to delineate intraspecific genetic relationships among bacteria. In this study, we have modified and evaluated a PCR-based technique, amplified fragment length polymorphism (AFLP) analysis, for use in fingerprinting strains of Staphylococcus aureus. Single-enzyme amplified fragment length polymorphism (SE-AFLP) analysis was used to perform strain identification of Staphylococus aureus. By careful selection of AFLP primers, it was possible to obtain reproducible and sensitive identification to strain level. AFLP fingerprinting of 5 reference strains of Staphylococcus aureus and 65 strains of Staphylococcus aureus that were isolated from food sources of different area and diverse genomic types of Staphylococcus aureus were recognized. As a result of this study, we found that the AFLP patterns of Staphylococcus aureus isolated from Seoul, Taejeon and Gwang-Ju indicated the close relation with genetic similarity. The main purpose of this study was to find an alternative and reliable fingerprinting method to study the overall genetic diversity, using Staphylococcus aureus species as an example, and observed if the method can be successfully applied to all staphylococcal species.

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The Experimental Study on Anti-bacterial Potency of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus aureus (소염 약침액, 황련해독탕, 황련이 Staphylococcus aureus에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.87-92
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus aureus that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus aureus in Hwangryunhaedoktang disappear all sample. 3. MIC on Staphylococcus aureus in Coptidis rhizoma was 100%, $50\;{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The present author think that Soyum Herbal-acupuncture must be extracted a new method for anti-bacterial potency and Coptidis rhizoma was made Herbal-acupuncture for eye drops.

Studies on the Distribution of mecA Gene in Methicillin-resistant Staphylococcus aureus by Polymerase Chain Reaction (Methicillin 내성 포도구균의 PCR에 의한 mecA 유전자 분포 조사)

  • 이규식
    • Biomedical Science Letters
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    • v.5 no.1
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    • pp.131-133
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    • 1999
  • In order to the investigate epidemiological characteristics of methicillin-resistant Staphylococcus aureus (MRSA), 31 strains of Staphylococcus aureus were isolated from the equipments of two hospitals in Chonbuk. And their antimicrobial resistance patterns against 7 kinds of antimicrobial agents and the identification of MRSA by polymerase chain reaction (PCR) were studied. Seven strains among 10 strains of methicillin resistant Staphylococcus aureus showed 554 bp DNA which was a part of mecA gene in PCR analysis.

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Characterization of Plasmids of Chloramphenicol Resistant Staphylococcus aureus (클로람페니콜 내성 황색포도상구균의 플라스미드 동정)

  • Park, Jung-Hee;Lee, Jae-Yoon;Moon, Kyung-Ho
    • YAKHAK HOEJI
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    • v.52 no.1
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    • pp.33-36
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    • 2008
  • Staphylococcus aureus KH13 and Staphylococcus aureus KH28 were resistant to chloramphenicol, ampicillin, clindamycin, erythromycin, gentamicin, kanamycin, streptomycin, tobramycin, and norfloxacin. A plasmid (pKH13) and two plasmids (pKH14, pKH15) were isolated from Staphylococcus aureus KH13 and Staphylococcus aureus KH28, respectively and complete nucleotide sequences of three plasmids were determined. It was found that pKH13 and pKH15 mediated chloramphenicol resistance and pKH14 was a cryptic plasmid.

The Experimental Study on Anti-bacterial Potency of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus & Staphylococcus epidermidis (황련해독탕 약침액과 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.99-103
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\beta}^{\circ}$ according to density Hwangryunhaedoktang Herbal-acupuncture & extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone With change of volume. Results : 1. MIC on Staphylococcus aureus in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. Conclusions : The present author think that Hwangryunhaedoktang Herbal-acupuncture must be extract a new method for anti-bacterial potency.

The Experimental Study on Anti-bacterial Potency of Soyum Herbal-acupuncture on Staphylococcus aureus & Staphylococcus epidermidis (소염 약침액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.73-77
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture(100%, 10%, 1%, 0.1%). Antibacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus epidermidis in Soyum Herbal-acupuncture disappear all sample. Conclusions : The present author think that Soyum Herbal-acupuncture must be extract a new method for anti-bacterial potency.

The Experimental Study on Anti-bacterial Potency of Coptidis rhizoma extract on Staphylococcus aureus & Staphylococcus epidermidis (황련 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.19 no.2
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    • pp.71-76
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by flowing to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was 100%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was 100% & 10%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The Present author think that Coptidis rhizoma extract must be made a new eye drops.

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