• Title, Summary, Keyword: Spodoptera litura

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Pathogenicity and Multiplication of Entomopathogenic Nematode, Steinernema carpocapsae Weiser, on Been Armyworm, Spodoptera exigua (Hubner) and Tobacco Cutworm, Spodoptera litura(Fabricius) (파밤나방(Spodoptera exigua (Hubner))과 담배거세미나방(Spodoptera litura(Fabricius))에 대한 곤충병원선충(Steinernema carpocapsae Weiser)의 감염력 및 증식력)

  • 한상찬;이성섭;김용균
    • Korean journal of applied entomology
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    • v.38 no.3
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    • pp.255-260
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    • 1999
  • Pathogenicity and multiplication of entomopathogenic nematode, Steinernema carpocapsae Weiser, ere analyzed in two insect hosts, Spodoptera exiga (Hubner) and Sp. litura(Fabricius). The estimated ${LC}^{50}s$(lethal concentration of the infective juveniles to kill 50% of the host insect population) were not different between tow insect species or among their developmental stages on the filter paper assay, thought the actual numbers of the infected nematodes were varied among them. The significant variation, however occurred in nematode multiplication between tow inset hosts. Temperature also gave sosignificatn effect on nematode multiplication rate that it took 6 days after infection at $25^{\circ}C$, but did 12 days at $20^{\circ}C$ to show the maximal nematode population peak($\approx$500,000 infective juveniles(IJ) in a 5th instar larva of Sp. litura and $\approx$-100,000 IJ in a 5th instar larva of Sp. exigua).

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Isolation and Characterization of the C-type Lysozyme Gene from the Common Cutworm Spodoptera litura

  • Kim, Jong-Wan;Yoe, Sung-Moon
    • Animal cells and systems
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    • v.13 no.3
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    • pp.345-350
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    • 2009
  • We have isolated and characterized a new insect chicken type (c-type) lysozyme gene from the common cutworm, Spodoptera litura. The full-length cDNA of Spodoptera lysozyme is cloned by rapid amplification of cDNA ends PCR (RACE-PCR). The isolated cDNA consists of 1039 bp including the coding region for a 142-amino acid residue polypeptide, which included a signal peptide of 21-amino acid residue and a mature protein of 121-amino acid residue. The predicted molecular weight of mature lysozyme and its theoretical isoelectric point from amino acid composition is 13964.8 Da and 9.05, respectively. The deduced amino acid sequence of Spodoptera lysozyme gene shows the highest similarity (96.7%) to Spodoptera exigua lysozyme among other lepidopteran species. Amino acid sequence comparison with other the c-type lysozymes, Spodoptera lysozyme has the completely conserved $Glu^{32}$ and $Asp^{50}$ of the active site and eight Cys residues are completely conserved in the same position as that of other lepidopteran lysozymes.

Insecticidal Activity and Histopathological Effects of Vip3Aa Protein from Bacillus thuringiensis on Spodoptera litura

  • Song, Feifei;Lin, Yunfeng;Chen, Chen;Shao, Ensi;Guan, Xiong;Huang, Zhipeng
    • Journal of Microbiology and Biotechnology
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    • v.26 no.10
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    • pp.1774-1780
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    • 2016
  • Vegetative insecticidal proteins (Vips) are insecticidal proteins synthesized by Bacillus thuringiensis during the vegetative stage of growth. In this study, Vip3Aa protein, obtained by in vitro expression of the vip3Aa gene from B. thuringiensis WB5, displayed high insecticidal activity against Spodoptera litura aside from Spodoptera exigua and Helicoverpa armigera. Bioassay results showed that the toxicity of Vip3Aa protein against S. litura larvae statistically decreased along with the increase of the age of the larvae, with LC50 = 2.609 ng/cm2 for neonatal larvae, LC50 = 28.778 ng/cm2 for first instar larvae, LC50 = 70.460 ng/cm2 for second instar larvae, and LC50 = 200.627 ng/cm2 for third instar larvae. The accumulative mortality of 100% larvae appeared at 72 h for all instars of S. litura larvae, when feeding respectively with 83.22, 213.04, 341.40, and 613.20 ng/cm2 of Vip3Aa toxin to the neonatal and first to third instar larvae. The histopathological effects of Vip3Aa toxin on the midgut epithelial cells of S. litura larvae was also investigated. The TEM observations showed wide damage of the epithelial cell in the midgut of S. litura larvae fed with Vip3Aa toxin.

Economic Injury Levels for the Common Cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) on Soybean (콩 담배거세미나방(Spodoptera litura)의 경제적 피해수준)

  • Lee, Geon-Hwi;Bae, Soon-Do;Kim, Hyun-Joo;Park, Sung-Tae;Choi, Man-Young
    • Korean journal of applied entomology
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    • v.45 no.3
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    • pp.333-337
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    • 2006
  • The damage aspects of soybean by common cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) at different larval density and different growth stage of soybean were studied in soybean field. The percent yield reduction(Y) of soybean infested by different densities of S. litura (X, no. of larvae/plant) under outdoor conditions for a three week period were estimated by the following equations: (1) Y = 1.655X - 6.025 ($R^{2}=0.952$) for the R1 (flowering stage); (2) Y = 0.725X - 0.475 ($R^{2}=0.986$) for the R3 (beginning pod stage); and (3) Y = 0.635X - 1.325 ($R^{2}=0.986$) for the R5 (beginning seed stage). Based on the relationships between the densities of S. litura larvae and the yield index of soybean, the number of larvae (2nd-3rd instar) which caused 5% loss of yield (Tolerable injury level) was estimated to as approximately 6.7 for the R1, 7.5 for the R5, and 10.0 per plant for the R5, respectively. Average soybean leaf areas consumed by 1st, 2nd, 3rd, 4th, 5th and 6th larvae of Spodoptera litura during 24 hr at $28^{\circ}C$ was 0.3, 0.7, 2.6, 4.0, 20.1, and $55.8\;cm^{2}$, respectively.

Effects of RH 5849, an Ecdysone Agonist, against Feeding and Growth of Tobacco Cutworm(spodoptera litura Fabricius)Larvae (담배거세미나방(Spodoptera litura Fabricius) 유충의 섭식과 생장에 대한 곤충탈피호르몬길항제 RH 5849의 영향)

  • 박노중;장경수;조점래;조광연
    • Korean journal of applied entomology
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    • v.31 no.4
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    • pp.475-479
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    • 1992
  • The non-steroidal ecdysone agonist RH 5849 showed almost similar LC.o values( 18.1-26.5 ppm) at all stages of larval development of the tobacco cutworm, Spodoptera litura, when treated by a leaf-disk dipping method. The feeding-inhibition rate for the 4th instar larvae was dose-dependent, and simultaneously the weight gain of 3rd instar larvae ceased within 48 hour after feeding of the cabbage leafdisk dipped into RH 5849 4.2 ppm solution. The systemic larvicidal effect of RH 5849 was compared at cabbage and tobacco whole plant test. The $LC_{50}$ values below 20 ppm(mg/kg soil) was lasted for 15 days in cabbage, 30 days in tobacco respectively.

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Comparative Analysis of the Difference in the Midgut Microbiota between the Laboratory Reared and the Field-caught Populations of Spodoptera litura

  • Pandey, Neeti;Rajagopal, Raman
    • Microbiology and Biotechnology Letters
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    • v.47 no.3
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    • pp.423-433
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    • 2019
  • Midgut microbiota is known to play a fundamental role in the biology and physiology of the agricultural pest, Spodoptera litura. This study reports the difference in the larval midgut microbiota of field-caught and laboratory-reared populations of S. litura by performing 16S rDNA amplicon pyrosequencing. Field populations for the study were collected from castor crops, whereas laboratory-reared larvae were fed on a regular chickpea based diet. In total, 23 bacterial phylotypes were observed from both laboratory-reared and field-caught caterpillars. Fisher's exact test with Storey's FDR multiple test correction demonstrated that bacterial genus, Clostridium was significantly abundant (p < 0.05) in field-caught larvae of S. litura as compared to that in the laboratory-reared larvae. Similarly, bacterial genera, such as Bradyrhizobium, Burkholderia, and Fibrisoma were identified (p < 0.05) predominantly in the laboratory-reared population. The Bray-Curtis dissimilarity matrix depicted a value of 0.986, which exhibited the maximum deviation between the midgut microbiota of the laboratory-reared and field-caught populations. No significant yeast diversity was seen in the laboratory-reared caterpillars. However, two yeast strains, namely Candida rugosa and Cyberlindnera fabianii were identified by PCR amplification and molecular cloning of the internal transcribed space region in the field-caught caterpillars. These results emphasize the differential colonization of gut residents based on environmental factors and diet.

Biological Control of Tobacco Cutworm, Spodoptera litura Fabricius with Entomopathogenic Nematodes

  • Park, Sun-Ho;Yu, Yeon-Su;Park, Jae-Sung;Choo, Ho-Yul;Bae, Soon-Do;Nam, Min-Hee
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.2
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    • pp.139-143
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    • 2001
  • The efficacies of several entomopathogenic nematodes of Steinernema and Heterorhabditis spp. were examined against tobacco cutworm, Spodoptera litura Fabricius. H. bacteriophora HY showed 100% mortality after 20 h against 2nd instar of tobacco cutworm. In the case of 3-4th instar, S. carpocapsae PC, H. bacteriophora HY and S. monticola CR showed 100% mortality after 47 h. In the case of 5-6th instar, S. carpocapsae PC proved more effective than the others. Generally, the number of nematodes harvested as their size decreased. Also, the highest number of nematodes was obtained in the 5-6th instar of S. litura by H. bacteriophora HY, showing about $1.3{\times}10^6$ nematodes per larva. In vitro cultured S. carpocapsae PC showed 100% mortality after 73 h against 5-6th instar tobacco cutworm, indicating that nematodes produced in vitro can be potentially used for the biological control of S. litura instead of nematodes in vivo.

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Control Thresholds for Managing Common Cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) on Chinese Cabbage (배추에서 담배거세미나방(Spodoptera litura)의 요방제 수준 설정)

  • Choi, Duck-Soo;Kim, Do-Ik;Kim, Seon-Gon;Ko, Suk-Ju;Kang, Beom-Ryong;Kim, Sang-Soo
    • Korean journal of applied entomology
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    • v.50 no.3
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    • pp.215-220
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    • 2011
  • This study aimed to estimate control thresholds for managing common cutworm, Spodoptera litura Faricius (Lepidoptera: Noctuidae) at different larval densities and growth stages of Chinese cabbage in field conditions. The percent yield reduction (Y) of Chinese cabbage infested by different densities of S. litura (X, no. of larvae/100 plants) for three weeks were estimated by Y=-21.85X+1300 (R2=0.997) 5 days after transplanting and Y=-12.1X+1382 (R2=0.998) 20 days after transplanting. Based on the relationships between the densities of S. litura larvae and the yield index of chinese cabbage, the number of larvae (2nd to 3rd instar) which caused 5% loss of yield was estimated as 2.9/100 plants 5 days after transplanting, and 5.6/100 plants 20 days after transplanting.

Molecular Cloning and Structural Analysis of the Antibacterial Gene from the Common Cutworm, Spodoptera litura

  • Nam, Duk-Hwa;Tae, Gun-Sik
    • BMB Reports
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    • v.31 no.6
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    • pp.536-541
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    • 1998
  • The cDNA clone encoding the antibacterial peptide (SL-1) was isolated from the fat body of the common cutworm, Spodoptera litura, immunized with E. coli K12. The primary structure analysis revealed that its deduced amino acid sequence showed the characteristics of the cecropin family antibacterial peptides and that the amino acid residues highly conserved in the antibacterial peptides from moths and flies were also conserved, implying that SL-1 was a cecropin-like, and especially cecropin B-like, peptide. The predicted secondary structure of the mature SL-1 consists of three domains: (i) an amphiphilic ${\alpha}$-helical domain (Ile-4 to Gly-18); (ii) the hinge region (Gly-23 and Pro-24); and (iii) a hydrophobic domain (Ala-25 to IIe-38).

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Cold hardiness of Spodoptera litura Fabricius (Lepidoptera: Noctuidae) (담배거세미나방(Spodoptera litura Fabricius)의 내한성)

  • 김용균;박형근;송원례
    • Korean journal of applied entomology
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    • v.36 no.3
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    • pp.256-263
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    • 1997
  • Supercooling points (SCP) and cold tolerance of the tobacco cutworm, Spodol~rera litura Fabricius. were measured in response to subzero temperatures. SCPs were varied among developmental stages. Eggs showed the lowest SCP (-27$^{\circ}$C). Pupae and adults had the intermediate SCP(- 18$^{\circ}$C). The SCPs (- I0 to - 16$^{\circ}$C) of larvae increased with their ages. Lethal low temperature of each stage was higher than its SCP. Preexposure of eggs and larvae to a sublethal low temperature increased their survival capacities under lethal low temperatures. The sublethal temperature also induced the fifth instar larvae to increase hemolymph osmolality and to produce cold-induced proteins of apparent molecular weights of 20 and 27 kD. These results indicate that this species is classified into a freeze-susceptible insect.

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