• Title, Summary, Keyword: Solanaceae

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Enzyme Activity and Gene Expression of Cytochrome P450 Involved in Capsidiol Biosynthesis in Solanaceae Plants (가지과식물에서 Capsidiol 생합성에 관여하는 Cytochrome P450 유전자의 발현과 효소활성)

  • Kwon, Soon-Tae;Hasegawa, Paul
    • Korean Journal of Plant Resources
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    • v.21 no.2
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    • pp.139-143
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    • 2008
  • Enzyme activity and expression of cytochrome P450 gene involved in the pathway of capsidiol biosynthesis were compared in five different solanaceae plants such as red pepper, green pepper, tobacco, potato and egg plant. Base on genomic DNA and/or RT-PCR results, four solanaceae plants such as red pepper, green pepper, tobacco and egg plant possess P450 gene in the genome and specifically expressed by elicitor treatment. However, potato was appeared to have neither P450 nor cyclase gene in the genome. P450 genes did not show any expression in the plants under normal condition, but showed highly specific expression under elicitation condition in various organs and tissue such as leaf, root, stem and culture cells.

A Phenolic Glucoside and Steroidal Sapogenins of Solanum Iyratum (배풍등의 페놀성 배당체 및 스테로이드 사포게닌)

  • Kang, So-Young;Sung, Sang-Hyun;Park, Jong-Hee;Cho, Jung-Hee;Kim, Young-Choong
    • YAKHAK HOEJI
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    • v.44 no.6
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    • pp.534-538
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    • 2000
  • A phenolic glucoside and two steroidal sapogenins were isolated from the aerial part of Solanum Iyratum (Solanaceae). They were identified as 2-hydroxy-3-methoxybenzoic acid glucose ester (1), ${\Delta}^{3,5}$-deoxytigogenin ((25R)-spirosta-3,5-diene) (2), diosgenin (($3{\beta}$, 25R)-spirost-5-en-3-ol) (3), respectively by several spectroscopic methods including IR, MS and NMR. Compound 1 has not been previously isolated from Solanaceae plants. Compounds 2 and 3 are isolated from S. Iyratum for the first time in the present study.

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Isolation and Characterization of Steroids of Nutraceutical Value in Physalis minima

  • Misra Laxmi N.;Lal Pyare;Kumar Devinder
    • Preventive Nutrition and Food Science
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    • v.11 no.2
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    • pp.133-139
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    • 2006
  • There is great demand of nutraceutical secondary metabolites in the world so as to feed the population by improving agricultural production with new varieties of food crops but what is still more important is to add nutrition into the food. Physalis minima, plant of Solanaceae family, contain certain secondary metabolites which enhance the over all metabolic functions of the body. Withasteroids are one of such phytochemicals that are generally regarded as safe. These chemicals are almost monopoly of the plants of Solanaceae. The leaves, stem and roots of P. minima have been examined to yield several steroids, identification of which has been discussed in this paper. These withasteroids contribute to the potential nutraceutical and health function value since incorporation of withanolides in the diet may prevent or decrease the growth of tumors in humans.

Development of RT-PCR Based Method for Detecting Five Non-reported Quarantine Plant Viruses Infecting the Family Cucurbitaceae or Solanaceae

  • Lee, Jong-Seung;Cho, Won-Kyong;Lee, Su-Heon;Choi, Hong-Soo;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.27 no.1
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    • pp.93-97
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    • 2011
  • For quarantine purpose, we selected five plant RNA viruses including Cucumber vein yellowing virus (CVYV), Cucurbit yellow stunting disorder virus (CYSDV), Potato aucuba mosaic virus (PAMV), Potato yellow dwarf virus (PYDV), and Tomato chlorosis virus (ToCV), which are not reported in Korea and cause serious economic losses to the family Cucurbitaceae or Solanaceae. To detect those viruses, we employed RT-PCR technique with specific oligonucleotide primer pairs and tested their detection efficiency for each virus. To design RT-PCR primers, coat protein was used for CVYV, CYSDV, and ToCV whereas RNA polymerase and nucleocapsid regions were used for PAMV and PYDV, respectively. The development of an RT-PCR based method proved a useful tool for rapid detection and identification of quarantine virus infections.

Studies on the Alkaloidal Components of the Fruits of Lycium chinense

  • Han, Byung-Hoon;Park, Jeong-Hill;Park, Myung-Hwan;Han, Yong-Nam
    • Archives of Pharmacal Research
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    • v.8 no.4
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    • pp.249-252
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    • 1985
  • $N_{9}$-Formylharman, 1-carbomethoxy-$\beta$-carboline and perlolyrine with an unidentified alkaloid ($C_{10}H_{13}NO_{2}$, $M^{+}$ 179.094) have been isolated from the fruits of Lycium chinense Miller (Solanaceae) and characterized on the basis of chemical and physical evidence.

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Comparative seed morphology of Solanaceae in Korea (한국산 가지과의 종자 비교형태)

  • Kong, Min-Jung;Lee, Jong-Su;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.41 no.1
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    • pp.35-46
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    • 2011
  • The seed morphology of 22 taxa (12 genera) of Solanaceae in Korea was studied by stereo and scanning electron microscopy, and a detailed description of seed morphological features for all examined taxa is provided. The color of the seed is yellow to black, and the shape is reniform to circular. Its size is 0.56-4.29 mm in length and 0.38-3.20 mm in width. The largest sample was found in Datural metel, while the smallest was in Petunia hybrida. Three different surface types (verrucate, verrucate-reticulate, and reticulate) were recognized in the studied taxa. The top of the anticlinal wall produces appendages known as 'fibrils'. This projection of anticlinal wall was found to be specific to the genus Solanum (S. japonense, S. lyratum, and S. lycopersicum). The morphological features (seed shape, size, color, hilum shape, surface type, and fibrils) of the family Solanaceae in Korea are described and their systematic implications are briefly discussed.

Detection of the Causal Agent of Bacterial Wilt, Ralstonia solanacearum in the Seeds of Solanaceae by PCR (가지과 종자에서 Ralstonia solanacearum의 검출을 위한 PCR 방법)

  • Cho, Jung-Hee;Yim, Kyu-Ock;Lee, Hyok-In;Baeg, Ji-Hyun;Cha, Jae-Soon
    • Research in Plant Disease
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    • v.17 no.2
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    • pp.184-190
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    • 2011
  • Ralstonia solanacearum, a causal agent of bacterium wilt is very difficult to control once the disease becomes endemic. Thus, Ralstonia solanacearum is a plant quarantine bacterium in many countries including Korea. In this study, we developed PCR assays, which can detect Ralstonia solanacearum from the Solanaceae seeds. Primers RS-JH-F and RS-JH-R amplified specifically a 401 bp fragment only from Ralstonia solanacearum race 1 and race 3. The nested PCR primers, RS-JH-F-ne and RS-JH-R-ne that were designed inside of 1st PCR amplicon amplified specifically a 131 bp fragment only from Ralstonia solanacearum race 1 and race 3. The primers did not amplify any non-specific DNA from the seed extracts of the Solanaceae including tomato and pepper. When detection sensitivity were compared using the Solanaceae seeds inoculated with target bacteria artificially, the nested PCR method developed in this study 100 times more sensitive than ELISA and selective medium. Therefore, we believe that the PCR assays developed in this work is very useful to detect Ralstonia solanacearum in the Solanaceae seeds.

Some Sesquiterpenoids and $5{\alpha},8{\alpha}-$Epidioxysterols from Solanum lyratum

  • Yu, Su-Mi;Kim, Hyoung-Ja;Woo, Eun-Rhan;Park, Ho-Koon
    • Archives of Pharmacal Research
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    • v.17 no.1
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    • pp.1-4
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    • 1994
  • From the stems of Solanum lyratum Thunb.(Solanaceae), two sesquiterpenoids together with two 5$\alpha$, 8$alpha-epidioxy sterols have been isolated and identified as atractylenolide I, dehydrocarissone, engosterol peroxide, 9, 11-dehydroergosterol peroxide. These compounds never previously isolated from this genus.

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Studies on the Chemical Components and Antioxidative Effect of Solanum lyratum Thunb (배풍등의 화학성분 및 항산화 효과에 관한 연구)

  • Shim, Kyung-Hee;Young, Han-Suk;Lee, Tae-Woong;Choi, Jae-Sue
    • Korean Journal of Pharmacognosy
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    • v.26 no.2
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    • pp.130-138
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    • 1995
  • Phytochemical study on the aerial parts of Solanum lyratum (Solanaceae) was carried out. On the basis of phytochemical and spectroscopic evidences, compound I was identified as mixtures of hexadecanoic acid methyl ester, 2,6,10,15-tetramethyl heptadecane, tricosane, tetracosane, pentacosane, docosanoic acid methyl ester, docosane, tricosanoic acid methyl ester, 8-hexyl pentadecane, tetracosanoic acid methyl ester, pentatriacontane, hexatriacontane, eicosane, hexacosane, hentriacontane and stigmasta-5,23-dien $3-{\beta}-ol$, and compound II, III, IV and V were identified as hexacosanoic acid methyl ester, ${\beta}-sitosterol-{\beta}-{_D}-glucoside$, $3-O-{\alpha}-{_L}-rhamnopyranosyl-(1->2)-{\beta}-{_D}-glucuronopyranosyl$ diosgenin and $3-O-{\alpha}-{_L}-rhamnopyranosyl-(1->6)-{\beta}-{_D}-glucopyranosyl$ quercetin (rutin), respectively. Rutin was identified as one of the active principles having antioxidative effect from S. lyratum.

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