• Title, Summary, Keyword: Selaginella tamariscina

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Molecular-vased sensitivity of human leukemia cell line U937 to antineoplastic activity in a traditional medicinal plants(Selaginella tamariscina) (전통 약용 식물 권백(Selaginella tamariscina)의 항암효과에 대한 혈액 암세포주 U937의 감수성 및 그 작용기구에 대한 분자생물학적 연구)

  • 이인자;이인선;박성희
    • Journal of Food Hygiene and Safety
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    • v.11 no.1
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    • pp.71-75
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    • 1996
  • In order to study the antitumoral effect of Selaginella tamariscina extracts, the cytotoxicities to human histiocytic leukemia cells (U937) and lymphocyte were measured by MTT method. The water extract of Selaginella tamariscina was partitioned into chloroform (CHCl3), ethylacetate (EtAc), n-butanol (BuOH) and water (H2O), successively. CHCl3, EtAc and BuOH fractions of Selaginella tamariscina showed the cytotoxicity to the U937 cells but they had effect on the cytotoxicity of lymphocyte under the same conditions. The tumor-specific cytotoxicity of Selaginella tamariscina fractions migh have been attributed to their genotoxic effect on actively proliferating cells. The expression of p53 tumor suppressor gene was then evaluated by northern blotting. The increased expression of p53 was induced by Selaginella tamariscina fraction V but no expression of p53 was induced by CHCl3, EtAc, and BuOH fractions of Selaginella tamariscina water extract (fraction V) should be required for the cytotoxcity on U937 and the other fractions of Selaginella tamariscina mediated the U937 disruption.

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Effects of Selaginella Tamariscina on Apoptosis via the Activation of Caspase-3 in HL-60 (권백의 Caspase-3 활성화를 통한 HL-60 세포에서 세포사멸 유도효과)

  • Nam Hang Woo;Lee Sung Won;An Byung Sang;Chough Won Joon;Kim Yeong Mok;Mun Yean Ja;Ahn Seong Hun;Woo Won Hong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.3
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    • pp.751-758
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    • 2003
  • In our previous studies, we reported that Selaginella Tamariscina(ST) induced apoptotic cell death in HL-60 cells selectively. The cell viability after treatment with extract of ST was quantified by MTT assay and trypan bleu exclusion method. The results showed that application with ST in HL-60 induced 40% cell death at the concentration of 400 ㎍/ml. The cancericidic effect of Selaginella Tamariscina was mediated by apoptosis. Thus, HL-60 cells exposed to Selaginella Tamariscina displayed the DNA fragmentation ladder and nucleus chromatin condensation characteristic for apoptosis. The enzyme activity of caspase-3 and actived caspase-3 protein were markedly increased in HL-60 cells treated with the extract of Selaginella Tamariscina. In addition, the extract of Selaginella Tamariscina induced cleavage of PARP, a known substrate for caspase-3. The expression of Bcl-2, anti-apoptotic protein, was decreased by treatment of the aqueous extract of Selaginella Tamariscina in a dose-dependent manner. And the expression of pro-apoptotic Bax protein was increased. In conclusion, our results suggest that the extract of Selaginella Tamariscina may induce the apoptotic death of HL-60 cells via activation of caspase-3, cleavage of PARP protein, depletion of cellular ATP levels and Bcl-2 degradation.

The Effect of Selaginella tamariscina on Inhibition of Pancreatic Lipase and Lipid Accumulation (부처손(Selaginella tamariscina) 추출물의 리파아제 저해 활성 및 지질 축적 억제 효과)

  • Kim, Gun-Hee;Lee, Shin-Young;Lee, Ae-Rang
    • The Korean Journal of Food And Nutrition
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    • v.32 no.1
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    • pp.27-32
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    • 2019
  • The objective of this study was to evaluate novel usability as natural anti-obesity supplement of Selaginella tamariscina extract. The total phenol contents and total flavonoid contents were $60.29{\pm}3.11GAE\;mg/g$ and $14.90{\pm}0.34QE\;mg/g$, respectively. To evaluate anti-obesity activity of Selaginella tamariscina extract, pancreatic lipase inhibition activity as well as its inhibition effect of lipid accumulation in adipocytes were conducted by Oil Red O staining and lipolysis assay. The result of pancreatic lipase inhibition activity of S. tamariscina extract showed a wide range between 40 and 73% dose dependently. While the incubation of 3T3-L1 cells with S. tamariscina extract inhibited differentiation of preadipocytes and reduced lipid accumulation, the level of released free glycerol into culturing medium was increased in multiple concentrations. These results showed that S. tamariscina extract inhibit adipogenesis and pancreatic lipase activity. Thus, S. tamariscina extract can be a candidate for regulating lipid accumulation in obesity.

Chemical Constituents of Selaginella tamariscina (권백의 화학성분)

  • Shin, Dong-In;Kim, Jin-Woong
    • YAKHAK HOEJI
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    • v.38 no.6
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    • pp.683-686
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    • 1994
  • The chemical constituents of Selaginella tamariscina were studied. From the n-hexane, chloroform and n-butanol soluble fractions, three compounds were isolated by chromatographic purification process. They were identified as lutein, $3{\beta}-cholesterol$ and hinokiflavone by spectral anaylsis(IR, MS, UV, $^1H-NMR$, $^{13}C-NMR$ and DEPT). Lutein and $3{\beta}-cholesterol$ were first isolated compounds from Selaginellaceae and hinokiflavone was a reported compound from this plant.

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Flavonoid Constituents of Selaginella tamariscina (권백의 Flavonoid 성분)

  • Shin, Dong-In;Kim, Jin-Woong
    • Korean Journal of Pharmacognosy
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    • v.22 no.4
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    • pp.207-210
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    • 1991
  • From the chloroform and n-butanol extracts of Selaginella tamariscina, three biflavonoids were isolated by chromatographic separation. Structures of these compounds were determined as cryptomerin B, amentoflavone and isocryptomerin by spectroscopic analysis, and amentoflavone was further identified by comparison with the authentic sample. This is the first report of isolation of cryptomerin B from Selaginellaceae.

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Study on Selective Anti-cancer Effects by the Water-extracts of Selaginella Tamariscina in Cancer Cell lines (권백물추출물의 선별적인 항암작용에 대한 연구)

  • Lee Sung Won;Ahn Seong Hun;Gwak Gun Sin;Kwak Sup;Lee Jang Cheon;Mun Yean Ja;Woo Won Hong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.6
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    • pp.1190-1196
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    • 2002
  • Selaginella Tamariscina is widely used in the traditional oriental herbal medicine for its anti-inflammatory, anti-cancer effects. The effects of aqueous extracts of Selaginella Tamariscina (ST) on the cell viability and induction of apoptotic cell death were investigated in A549, Raw 264.7, C6-glioma. Jurkat and HL-60 cells. The cell viability after treating with extract of Selaginella Tamariscina was quantified by MTT assay method. The results showed that ST decreased the cell viability in HL-60 and Jurkat cells not in A549, Raw 264.7 and C6-glioma cells. And we also observed the chromatin condensation and DNA fragmentation in HL-60 and Jurkat cells. The enzyme activity of caspase-3, tightly regulated by an apoptosis activating complex, were markedly increased in HL-60 cells treated with the ST by dose-dependent manner. In conclusion, our results suggest that the extract of Selaginella Tamariscina may induce the selective apoptotic cell death in HL-60 and Jurkat cells via activation of caspase-3.

Selaginella tamariscina Extract Improves Scopolamine-induced Learning and Memory Dificits in Rats (부처손 추출물의 치매개선 효과 및 기전탐색)

  • Chu, Soon-Ju;Heo, Jin-Sun;Sohn, Kie-ho
    • Korean Journal of Pharmacognosy
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    • v.47 no.4
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    • pp.319-326
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    • 2016
  • We investigated the effect of Selaginella tamariscina extract on the learning and memory impairments in scopolamine-induced (5 mg/kg, i.p.) dementia rats. Rats treated with oral tacrin (20 mg/kg, p.o.) as positive control group and S. tamariscina extract 100, 200mg/kg, p. o. (SME 100, SME 200) as experimental group had significantly reduced scopolamine-induced memory deficits in the passive avoidance test. The acetylcholine content were paralleled the results of the behavior experiment. The acetylcholine contents of the experimental groups (SME 200 group) was higher than that of control group. We also evaluated expression of VAchT, vesicular acetylcholine transporter. SME was significantly increased VAchT expression on hippocampus of scopolamine-induced dementia rats. We suggest that S. tamariscina might exert a significantly neuro-protective effect on cognitive impairment.

Effect of Selaginella tamariscina on U937 Cytoxicity (권백이 U937의 세포독성에 미치는 영향)

  • 박성희;이인자
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.5
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    • pp.799-804
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    • 1994
  • In order to study the antitumoral effect of Selaginella tamariscina extract, the cytotoxicities to human histiocytic lymphoma (U937) and lymphocyte were measured by MTT method. The water extract of Selaginella tamariscina showed the effective cytoxicity and increased the cytotoxicity of doxorubicine, cyclophosphamide on U937, but it has no effect on the cytotoxicity of lymphocyte. The cytotoxicity increased with the addition of other antineplastic agents but decreased with the combination of antineoplastic agent and Selaginella tamariscina in the lymphocyte. The results indicted that the side actions of retinoic acid, doxorubicine and cyclophosphamide decreased by addition of Selaginella tamariscina water extracts.

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Antioxidation and Inhibition of Matrix Metalloproteinase in UV-irradiated Human Dermal Fibroblast by Selaginella tamariscina (자외선이 조사된 사람 피부 섬유아세포에서 권백의 항산화와 MMP 발현에 미치는 영향에 대한 연구)

  • Sim, Gwan-Sub;Kim, Jin-Hwa;Kim, Jin-Hui;Lee, Bum-Chun;Pyo, Hyeong-Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.32 no.2
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    • pp.75-79
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    • 2006
  • In this study. we evaluated anti-aging activity of medical plants that protect the skin cell damage induced by UV irradiation. We have investigated diverse biological activities of Selaginella tamariscina as an anti-aging ingredient of cosmetics. S. tamariscina was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the $IC_{50}$ values of $65.1{\mu}g/mL$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and $40.9 {\mu}g/mL$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. For testing intracellular ROS scavenging activity, the cultured human dermal fibroblasts were analyzed by increase in dichlorofluorescein (DCF) fluorescence upon exposure to UVB $20 mJ/cm^2$ after treatment of S. tamariscina. UVA-induced MMP-1 protein and mRNA expression in human dermal fibroblasts were reduced in a dose-dependent manner by S. tamariscina. Moreover, S. tamariscina inhibited MMP-2 (gelatinase) activity in UVA-irradiated human dermal fibroblasts assayed by zymography and semi-quantitative RT-PCR. Taken together, these results suggest that S. tamariscina may act as an anti-aging agent by Increasing collagen and preventing the skin cell damage induced by UV irradiation, and imply that S. tamariscina nay be useful as a new ingredient for anti-aging cosmetics.

Cytotoxicity of Folkloric Medicine in Murine and Human Cancer Cells (천연물로부터 항암물질의 분리)

  • Lee, Ihn-Rhan;Song, Ji-Young;Lee, Yun-Sl
    • Korean Journal of Pharmacognosy
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    • v.23 no.3
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    • pp.132-136
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    • 1992
  • The whole plants of Selaginella tamariscina, Orostachycis japonicus, the cortex of Ulmus mandshurica, and the wood of Alnus japonica have been used as folk medicine for treating cancer. The cytotoxic activity of these plants were tested using a calorimetric tetrazolium assay (MTT assay). S. tamariscina and A. japonica showed mild $IC_{50}$ value, comparing with O. japonicus and U. mandshurica. So, MeOH extracts of S. tamariscina and A. japonica were partitioned into $CHCl_3$, EtOAc and n-BuOH, successively. The $CHCl_3$, EtOAc and BuOH fractions of S. tamariscina and A. japonica showed low percent of survival against $P_{388}$ and $MKN_{45}$ cells respectively. To isolate active components, they were subjected to silica gel column chromatography. Compound I was obtained from EtOAc extracts of S. tamariscina and identified as amentoflavone by chemical and spectral analysis. Amentoflavone inhibited the survival of P388 cells dose dependently, while not clearly inhibited that of $MKN_{45}$ cells.

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