• Title, Summary, Keyword: Seed germination

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Initiation of Germination Characters of Plant Seed by Light Quality (광에 의한 종자의 발아특성 발현과 그 분화 및 형성에 관하여)

  • 최관삼
    • Proceedings of the Botanical Society of Korea Conference
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    • pp.175-190
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    • 1987
  • Germination characters of the lettuce seed that received photoperiodic pretreatments of low or high temperature from the flowering to harvest. MSU-15 seed, one of the lettuce cultivars used, having high dark germination, was modified to low dark germination by the long-day treatments during the seed formation. Light-requiring MSU-16 seed was modified to the dark-germination seed by high temperature given at the seed formation period. Above results suggest that the environmental conditions given to an immature seed adhered to a mother plant bring about some modification to its native germination habit. I confirmed that the spectral quality of light could influence the phytochrome system which controlled germination characters of the progeny of lettuce seed ; plants grown in light rich in far-red energies produced light-requiring seed, but those grown in high deficient in far-red energies produced dark germination seed.

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Effects of Seed Germination on Characteristics of Perilla Seed Lipids (들깨의 발아가 들깨지방질의 특성에 미치는 영향)

  • Choe, Eun-Ok;Hwang, Hyun-Suk
    • Korean journal of food and cookery science
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    • v.27 no.3
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    • pp.51-57
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    • 2011
  • Color, lipid and fatty acid composition, and tocopherols and polyphenols contents of perilla seed lipids in response to seed germination were studied. Perilla seeds were germinated at $30^{\circ}C$ in the dark for 12, 36, or 48 h, after which total lipids were extracted by the Folch method using chloroform and methanol (2:1, v/v). Seed germination resulted in a decrease in yellowness and greenness in perilla seed lipids, but there were no significant changes in composition of the lipids including major neutral lipids (>90%). Contents of phosphatidylcholine and phosphatidylethanolamine in the perilla seed lipids significantly increased in response to germination. Linolenic acid (>63%) was the most abundant fatty acid. Seed germination tended to decrease the relative content of linolenic acid and increase the contents of oleic and stearic acids. Contents of antioxidants, especially ${\alpha}$-tocopherol and polyphenols, increased in response to seed germination. As the germination period was extended, the antioxidant content increased. Therefore, increases in useful components, phosphatidylcholine, phosphatidylethanolamine, ${\alpha}$-tocopherol, and polyphenols contents by seed germination can contribute to the improvement of perilla seed utilization in food industry.

Removal Effect of Biostone and Green Tea on the Heavy Metal Toxicity during Seed Germination of Arabidopsis thaliana (애기장대의 종자 발아에 미치는 맥반석과 녹차의 중금속 제거 효과)

  • 박종범
    • Journal of Environmental Science International
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    • v.12 no.12
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    • pp.1303-1308
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    • 2003
  • This experiment was carried out to investigate the effects of heavy metals (cadmium, chromium, copper and lead) on the seed germination of Arabidopsis thaliana, and examinated the removal effects of biostone and green tea on the heavy metal toxicity. Cadmium and chromium among the four heavy metals had no effect on the seed germination even in the concentration fifty times higher than in the official standard concentration of pollutant exhaust notified by the Ministry of Environment. However, seeds were not germinated in the concentration of copper ten times higher and in the concentration of lead fifty times higher than the official standard concentration. When seeds were sown in the solutions of lead (15, 20, 25 and 30 mg/L) and copper(15 and 20 mg/L), the seed germination rates were 0% and less than 10%, respectively. However, when biostone(3 g/30 $m\ell$) was added, the seed germination rate was 100% in all the concentrations. The germination rate was 100% in distilled water and copper solution (5 mg/L). However, green tea (0.2 g/30 $m\ell$) was added, the seed germination rate was 0% in both. The results show that cadmiun and chromium had no effect on the seed germination, but lead and copper decreased the rate of seed germination of Arabidopsis thaliana, Biostone removed heavy metal toxicity, but green tea did not removed heavy metal toxicity during germination.

The Effect of Seed Coat Scarification with Sodium Hypochiorite on Germination of Zoysiagrass Seed (Zoysiagrass 종자 발아에 미치는 Sodiym Hypochlorite의 종피처리 효과)

  • 구자형;김태일;원동찬
    • Asian Journal of Turfgrass Science
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    • v.3 no.2
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    • pp.89-94
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    • 1989
  • Research was conducted to obtain t he optimum treatment Of sodium hypochlorite(NaOC I) at various temperatures in t he seed scarification for stimulating germination of zoysiagrass (Zocysia Japonica Steud ) seed. Morphological changes of seed coat were also examined by scanning electron micros cop(SEM). l. Differences in temperature of scarification with 2 .4% NaOCI showed little influence on promoting germination of seeds but seeds treated with 1% solution at l5˚C germinated less than that of higher temperatures. The promotion effect of 4% solution on germination was diminished when seeds were treated for 8 hours of more. The most favorable seed scarification unaffected hy temperature for enhancing germination was 4-6hours treatment at 4% solution in fresh seeds. 2. $GA_3$, treatment did not enhance germination of water-pretreated control seeds hut germination of seeds pretreated with NaOCI l was increased additional 10% or more hy$ GA_3$, Water pretreated control seeds treated with 50 mM hydrogen peroxide(H'O )germinated about 44%. In NaOCI treated seeds. $H_2$$O_2$ treatment increased germination additional l 0% or more. 3. NaOCI l and KOH treatment softened the seed coat and formed the pores hy removing the scab-like thikenings attached to the seed surface. These results suggest that the modes of action of NaOCl in the promotion of seed germination reside in it increase of the permeability of the seed coat, and in the provision of additional oxygen to the seed.

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Studies on Seed Germination Characteristics and Patterns of Protein Expression of Lithospermum erythrorhizon by Plant Growth Regulators and Seed Primings (생장조절제와 프라이밍 처리에 의한 지치종자의 발아특성과 단백질 발현 양상에 관한 연구)

  • Kim, Do Hyun;Ahn, Bok Ju;An, Hee Jung;Ahn, Young Sup;Kim, Young Guk;Park, Chun Geun;Park, Chung Beom;Cha, Seon Woo;Song, Beom Heon
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.6
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    • pp.435-441
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    • 2014
  • This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of managing and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvesting seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of $GA_3$ and Kinetin was affected to improve the seed germination. $GA_3$ increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germination rate with increasing their levels, whereas $KNO_3$ increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showing more and clearer bands were positively related to the germination rates which were different by seed colors, storage temperatures and durations, and plant growth regulators and primings.

Seed Dormancy and Germination Characteristics of Annual Bluegrass (Poa annua L.) (새포아풀(Poa annua sp.)의 종자휴면과 발아특성)

  • 김태준;송재은;최정섭;조광연
    • Asian Journal of Turfgrass Science
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    • v.15 no.1
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    • pp.15-20
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    • 2001
  • Two types of annual bluegrass have been reported, and those consist of annual type (Poa annua ssp. annua) and perennial type (Poa annua ssp. reptans). As a weed, annual bluegrasses are commonly found in putting greens and fairways in many golf courses. Due to its strong competitiveness such as tremendous seed reproduction rate a year, prostrate growth habit, and no herbicide availability, annual bluegrasses have been considered as one of the most hard-to-control weeds in turf management systems. A growth chamber study was conducted to determine seed dormancy and to understand seed germination characteristics of annual bluegrass (Poa annua ssp. annua). Freshly harvested seeds showed 80 and 55% germination at 30 and $35^{\circ}C$, respectively, indicating that the optimum temperature of annual bluegrass is $30^{\circ}C$. However, the seed germination occurred only under light condition at any given temperature. This result demonstrated that light is prerequisite for the seed germination, and no primary dormancy of annual bluegrass seed exists. Secondary seed dormancy induced by unfavorable temperatures and dark condition was broken through 4 to 6 wk-storage at $4^{\circ}C$ with moisture, and the stored seeds germinated at $20^{\circ}C$ even under the dark. In red and far-red light trial, fresh seeds resulted in 40% germination under red while no seed germination occurred under far-red light condition, indicating that phytochrome Pr and Pfr could be related to annual bluegrass seed germination. When the far-red light replaced the red the germination was recovered, but this reversibility did not reach to the germination level under the red light only. This result implied that other lights than red and far-red would play an important role on seed germination of annual bluegrass.

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Studies on Seed Germination of Miscanthus sinensis Native to Jeju Island (제주도 자생 참억새의 종자발아에 관한 연구)

  • Lee, Jong Suk;Han, Seung Won
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.1
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    • pp.9-15
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    • 2007
  • The study was conducted to find out the harvesting time, storage duration, cold treatment and sterilization on seed germination of Miscanthus sinensis. The optimum harvesting time of the Miscanthus seed in native to Jeju island was December. And the seed germination passible 3 years after harvesting. When the seeds had cold treatment, seed germination nate was 74% rather than 63% of non-treatment. For seed sterilization treatment, soaking in solution of Benomyl 7% during from 3 hours to 12 hours germinated 9% over. But the non-treatment on 24 hours treatment reduced the seed germination of Miscanthus sinensis.

Studies on Establishment of Oversown Pasture Seed I. Effects of coating materials and minerals on germination (겉뿌린 목초종자의 정착에 관한 연구 I. 각종 증량재 및 미량광물질의 종자피복이 발아에 미치는 영향)

  • 이효원;정병용;김희경
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.7 no.2
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    • pp.113-119
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    • 1987
  • This experiment was carried out in laboratory to find out the effect of coating materials, minerals and insecticides on germination of pasture seed. Seed coating was made by specially made machine and seed germination in petridishes was also determined from Sep. 1986 to May 1987. The results were summarized as follows; 1. Coating seed gave bad germination compared with the control. There were significant difference between treatment, coating materials and adhesives in white and red clover while Phleum pratense germination was improved by coating materials. 2. In mineral coating treatments, on Mg alone or Mg mixed treatment, germination was deepressed. Generally germination was lowed by mineral treatment, but the difference was small. 3. Uncoating treatment with insecticide was superior to coating treatment in terms of germination. Among the insecticide Ballen gave more serious effect to seed germination.

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Effect of Pre-sowing Treatments on Seed Germination and Seedling Growth of Canarium resiniferum, A Rare Native Tree of Bangladesh

  • Hasnat, G.N. Tanjina;Hossain, Mohammed Kamal;Alam, Mohammed Shafiul;Hossain, Md. Akhter
    • Journal of Forest and Environmental Science
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    • v.33 no.3
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    • pp.226-232
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    • 2017
  • Dhup (Canarium resiniferum) is an economically and aesthetically important rare native tree species of Bangladesh. In natural condition 78.5-98.7% seeds do not germinate due to inhibition by hard seed coat, seed predation and unfavorable environmental conditions. A study was conducted in the Seed Research Laboratory and nursery of Institute of Forestry and Environmental Sciences, Chittagong University, Bangladesh in 2013 to find out appropriate pre-sowing treatments for maximizing germination and initial seedling growth. Eleven pre-sowing treatments were provided in both the seeds sown in polybags and seeds sown in propagator house. Results revealed that, germination started at first (after 20 days of seed sown) in seeds immersed in water at room temperature for 24 hours and germination completed within 38 days. Significantly higher (p<0.05) germination percentage (33%), germination energy (16.7%), plant percent (33%) and germination value (0.4) was found with seeds immersed in water at room temperature for 24 hours. Seedling height measured at three, four and five months after the seed germination in case of seeds treated by immersion in water for 24 hours was also greater than others. Therefore, pre-sowing treatment by immersion in water at room temperature for 24 hours was more effective in germination and production of quality vigor seedlings of Dhup.

Effects of PEG Priming Treatment on Germination and Seedling Growth of Onion Seed(Allium cepa L.)

  • Lee, Sheong-Chun;Ahn, Chan-Young
    • Plant Resources
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    • v.2 no.2
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    • pp.88-95
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    • 1999
  • These experiments were conducted to evaluate the variability of seed germination and seedling growth with different levels of polyethylene glycol(PEG 6,000) solution in onion seed. Average germination percentage of seed primed in PEG solution with 1.00 and 0.75 MPa was higher than control, and that of seed primed in 1.50MPa was lower than unprimed control. Germination percentage(GP) of seed primed for 5 days was highest, and as the primed days become long, the GP was decreased. The GP of airation seed during the primed was higher than that of unairation seed, about 5% , respectively. The GP of washed seed after primed was higher than that of unwashed seed, but that of redried seed after primed was lower than that of the others. The highest GP cultivar was Chunjoogoohyung and the lowest GP cultivar was Seouldego in unredried seed after primed, but Chunjoojoonggo was highest and Jungpoonwhang was the lowest cultivar in redried seed after primed. As the PEG concentration increased, the seedling length(SL) was shortened, and seed primed for 15 days was longer than other treatments. The SL of primed seed was similar to GP. The SL of washed seed after primed was longer than that of others, but that of redried seed after primed was shortest among the others. The SL of Chunjoojoonggo and Nongwoodego was longest and Seouldego was shortest among the cultivars in unredried seed after primed, but that of Chunjoogoohyung and Chunjoojoonggo was longest and Seouldego was the shortest cultivars in redried seed after primed.

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