• Title, Summary, Keyword: Secretin

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Plasma Secretin Concentrations in Fasting and Postprandial States of Normal Korean Subjects (정상 한국 성인의 공복시 및 식후 혈장 Secretin 농도)

  • Sim, Yeo-Rim;Jo, Yang-Hyeok;Sim, Sang-Soo;Nam, Sang-Chae;Kim, Myung-Suk
    • The Korean journal of physiology & pharmacology
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    • v.19 no.2
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    • pp.161-171
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    • 1985
  • This study was conducted to investigate fasting plasma secretin and postprandial secretin concentrations after ingestion of a protein meal or a sucrose solution in 20 healthy Korean subjects. In 12 subjects, ingestion of a protein meal, hamburger resulted in a significant and sustained increase in the mean plasma secretin concentrations, from mean fasting levels of less than 10 pg/ml to $12{\sim}16\;pg/ml$, and the mean plasma secretin concentrations, $9{\sim}13\;pg/ml$, after a rice meal increased significantly but transiently compared with mean fasting levels. The magnitude of postprandial increase in the Plasma secretin concentration after the hamburger was greater than that of the rice meal. In the remaining 8 subjects, drinking of a sucrose solution resulted also in a significant but transient increase in the mean Plasma secretin concentrations, from mean fasting levels of less than 10 pg/ml to $10{\sim}14\;pg/ml$ which were significantly greater than that after a physiological saline. Significant increase in the plasma secretin concentration was not observed during the postprandial period after the physiological saline. It is inferred from the above results that the Plasma secretin levels increase significantly after ingestions of a carbohydrate meal as well as a protein meal in the Korean race.

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Interaction between Cholecystokinin and Secretin in Isolated Rat Pancreatic Acini

  • Yoon, Shin-Hee;Hahn, Sang-June;Sim, Sang-Soo;Rhie, Duck-Joo;Song, In-Young;Baek, Hye-Jung;Kim, Myung-Suk;Jo, Yang-Hyeok
    • The Korean journal of physiology & pharmacology
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    • v.29 no.2
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    • pp.243-250
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    • 1995
  • A possible potentiation between cholecystokinin (CCK) and secretin in amylase secretion from isolated rat pancreatic acini was investigated. Combined treatment of acini with secretin and CCK at low concentrations, which are known to be physiological, resulted in enzyme secretion larger than the arithmetic sum of their separate effects. Such a potentiating effect also occurred between secretin and A23187 (Ca ionophore), between forskolin (adenylate cyclase activator) and CCK, and between forskolin and A23187. Staurosporin (protein kinase C inhibitor) and W7 (calmodulin antagonist) inhibited markedly the potentiated amylase release induced by the agonists, but KT5720 (protein kinase A inhibitor) did not affect the potentiated amylase release. Therefore, we concluded that the action of CCK in a physiological concentration is potentiated by secretin in a physiological concentration range and vice versa, and that the intracellular mechanism necessary for the potentiation is associated with $Ca^{2+}$. However, it is uncertain what mechanisms are involved in potentiation of amylase release after CAMP and $Ca^{2+}$.

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Roles of Gonadal Steroids on Exocrine Secretion of Isolated Perfused Rat Pancreas

  • Park, Hyung-Seo;Kim, Se-Hoon;Park, Hyoung-Jin;Lee, Mee-Young;Han, Young-Hee
    • The Korean Journal of Physiology and Pharmacology
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    • v.7 no.4
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    • pp.217-221
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    • 2003
  • To clarify the roles of gonadal steroids on pancreatic exocrine secretion, effects of progesterone and estradiol-$17{\beta}$ on spontaneous and secretagogue-induced exocrine response of isolated perfused rat pancreas were investigated. Intra-arterial infusion of progesterone resulted in significant increase of the spontaneous pancreatic fluid and amylase secretion dose-dependently. However, estradiol-$17{\beta}$ did not exert any influence on spontaneous pancreatic exocrine secretion. Exogenous secretin, cholecystokinin (CCK), and acetylcholine markedly stimulated pancreatic fluid and amylase secretion. Progesterone initially enhanced secretin-induced amylase secretion, but this stimulatory response declined thereafter to basal value. Moreover, secretin-induced fluid secretion was not affected by infusion of progesterone. Therefore, initial increase of secretion-induced amylase secretion by progesterone seems to be a non-specific action by washout effect of secretin. Estradiol-$17{\beta}$ failed to change the secretin-induced fluid and amylase secretion. Both progesterone and estradiol-$17{\beta}$ did not exert any influence on CCK-induced fluid and amylase secretion. Acetylcholine-induced exocrine secretion of isolated perfused pancreas also was not affected by intra-arterial infusion of progesterone or estradiol-$17{\beta}$. It is concluded from the above results that progesterone could enhance the spontaneous pancreatic fluid and amylase secretion of isolated perfused rat pancreas through non-genomic shortterm action, and that these effects could be masked by more potent stimulants such as secretin, CCK, and acetylcholine.

Effects of Intravenous Catecholamine on Gastric Acid, Gastrin and Secretin Secretion in Basal State of the Rat (정맥 주입한 Catecholamine이 흰쥐의 기초상태시 위산, Gastrin 및 Secretin 분비에 미치는 영향)

  • Kim, Myung-Suk;Sim, Sang-Soo;Kim, Mie-Hye;Choi, Hyun
    • The Korean journal of physiology & pharmacology
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    • v.22 no.2
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    • pp.179-187
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    • 1988
  • This study was conducted to investigate the effects of epinephrine and norepinephrine on basal gastric acid secretion and plasma gastrin and secretin concentration in the conscious rat. One hundred and eighty-four albino rats with gastric cannula were used after 18 hours or more of fast, with water ad libitum. In a restraint cage for collection of gastric juice, physiological saline (0.9% NaCl) was continuously infused into the jugular vein through a catheter for one hour at a rate of 1 ml/hr (control period). Immediately after the control period, epinephrine (1, 2, 4, 8 and $16{\mu}g/ml/hr)$, norepinephrine (1, 2, 4, 8 and $16{\mu}g/ml/hr)$ or physiological saline (1 ml/hr) was infused for another one hour. Gastric juice was collected at one hour interval for two hours infusion period. Adrenergic antagonists, phentolamine and propranolol were injected into the jugular vein 5 min prior to the infusion of epinephrine or norepinephrine at a dose of 0.2 mg/0.1 ml. Blood was sampled from the jugular vein for the radioimmunoassay of plasma gastrin and secretin after the collection of gastric juice. The results were as follows: 1) Both epinephrine and norephinephrine significantly increased gastric acid output in a dosedependent manner. 2) The effects of epinephrine and norepinephrine on the gastric acid secretion were antagonized by the pretreatment with phentolamine and propranolol. 3) Plasma gastrin and secretin concentrations were not significantly affected by the intravenous infusion of epinephrine and norepinephrine. It can be inferred from the above results that epinephrine and norepinephrine facilitate gastric acid secretion in conscious rats and the mechanism of which is attributed to ${\alpha}\;and\;{\beta}$ adrenergic receptors rather than gastrin and secretin.

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Cholinergic Control of Pancreatic Secretion: The Effects of Atropine on Plasma Cholecystokinin and Secretin Release

  • Jo, Yang-Hyeok;Rhie, Duck-Joo;Chang, Young-Soon;Hahn, Sang-June;Sim, Sang-Soo;Kim, Myung-Suk;Kim, Chung-Chin
    • The Korean journal of physiology & pharmacology
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    • v.25 no.1
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    • pp.27-35
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    • 1991
  • Generally, it has been known that cholecystokinin (CCK) release into the plasma is under cholinergic control, but secretin release is not. Thus in anesthetized dogs we studied the effect of atropine $(50\;{\mu}g/kg\;followed\;by\;50\;{\mu}g/kg/hr)$ on pancreatic secretion and plasma concentrations of bioactive CCK and immunoreactive secretin in response to intraduodenal perfusion of sodium oleate (1, 3 and 9 mmol/hr). The volume, protein output and bicarbonate output of the secretion were increased by sodium cleats and this oleate-induced secretion was decreased significantly by atropine administration. However the increased plasma CCK and secretin levels by sodium oleate were not changed by atropine. These results indicate that atropine suppressed sodium oleate-induced pancreatic secretion through inhibiting cholinergic mechanism directly rather than decreasing the release of pancreatic secretory hormones. In another set of experiments, bilateral cervical vagi were stimulated electrically to observe the changes of pancreatic secretion and the above two plasma hormone levels in the presence or absence of atropine. In the vagally stimulated dogs, the volume, protein output and bicarbonate output of the pancreatic secretion were increased significantly. Both plasma secretin and CCK were concomitantly released significantly by vagal stimulation. Atropine significantly depressed the pancreatic secretory response as well as the release of these two pancreatic secretory hormones. Therefore, we conclude that in the presence of atropine the depressed pancreatic response to vagal stimulation is at least, in part, due to decreased release of endogenous CCK and secretin. In the vagally stimulated animals, however, the involvement of direct cholinergic influence on pancreatic exocrine gland remains to be answered.

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Effects of Dansambohyultang on serum gastrin, secretin release and CNS (단삼보혈탕(丹蔘補血湯)이 혈청(血淸) gastrin, secretin 및 중추신경계(中樞神經系)에 미치는 영향(影響))

  • Yeo, Eun-Kyung;Yun, Sang-Hyup;Rue, Bong-Ha;Park, Dong-Won;Rue, Gi-Won
    • The Journal of Internal Korean Medicine
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    • v.19 no.2
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    • pp.233-248
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    • 1998
  • This study was conducted to investigate effects of Dansambohyultang on serum gastrin, secretin release and CNS in rats and mice. One group of seven rats was intactly in normal condition, the other group of seven rats was respectively heated, starved, and stressfully immobilized. The third group of seven rats was provided with Dansambohyultang before the stress, and the forth one after the stress. And, gastrin concentration and secretin concentration were measured. Mice were studied with regard to convulsion time, total sleep time and analegic effects on cental nerve system. The results were as follows: 1. Gastrin concentration was significantly decreased, and secretin concentration was significantly increased. 2. Analegic effect by acetic acid method was recognizably observed. 3. Effect of total sleep time done by pentobarbital-Na was recognizably observed. 4. Anti-convulsion effects induced by strychnine, picrotoxin and caffeine were recognizable. It is inferred from above results that Dansambohyultang inhibits gastin release, stimulates secretin release and palliate the pain.

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Effect of Damage to Medial Amygdaloid Nucleus on Pancreatic Exocrine Secretion Stimulated by Hydrochloric Acid in the Rat (흰쥐에서 내측 편도핵의 손상이 염산 자극에 의한 췌장 외분비에 비치는 영향)

  • Kim, Myung-Suk;Yoon, Shin-Hee;Hahn, Sang-June;Kim, Mie-Hye
    • The Korean journal of physiology & pharmacology
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    • v.22 no.2
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    • pp.273-280
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    • 1988
  • This study was undertaken to investigate the effect of the medial amygdaloid nucleus on the pancreatic exocrine secretion and plasma secretin concentration in 44 male albino rats. Twenty-three rats in which the medial amygdaloid nucleus was damaged bilaterally by radio frequency a.c. through stereotaxically inserted electrodes (medical amygdaloid group, MA) and twenty-one rats which received the same operation without damage (operated control, OC), were prepared. Under urethan anesthesia, 0.01 N hydrochloric acid (HCl) or physiological saline (0.9% NaCl) was infused at a rate of 0.18 ml/min into the duodenum for 20 minutes. Pancreatic jucie was collected for the 20 min infusion period. After collection of pancreatic juice, blood was sampled from the abdominal aorta for the radioimmunoassay of plasma secretin concentration. In the MA group, the exocrine pancreatic secretory response to 0.01 N HCI as well as saline infusion was significantly inhibited compared with that in the OC group. The pancreatic protein output of the MA group significantly decreased after the saline infusion and tended to decrease after the 0.01 N HCI infusion, compared with that of the OC group. However, there was no significant difference in plasma secretin concentration between the two groups. Therefore it is strongly suggested that the rat medial amygdaloid nucleus has a facilitatory influence on both basal and acid-stimulated pancreatic exocrine secretion, but the releasing mechanism of secretin appears not to be involved in the influence.

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Altered Secretory Pattern of Pancreatic Enzymes and Gastrointestinal Hormones in Streptozotocin-induced Diabetic Rats

  • Kim, Myung-Jun;Ryu, Gyeong-Ryul;Yi, Sae-Young;Min, Do-Sik;Rhie, Duck-Joo;Yoon, Shin-Hee;Hahn, Sang-June;Kim, Myung-Suk;Jo, Yang-Hyeok
    • The Korean Journal of Physiology and Pharmacology
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    • v.6 no.6
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    • pp.311-317
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    • 2002
  • This study was performed to investigate the pancreatic exocrine dysfunction in streptozotocin- induced diabetic rats. Changes in pancreatic enzymes secretion and in pancreatic enzymes content were observed. The output and the tissue content of amylase were significantly reduced in diabetic rats, while the output and the content of lipase were increased. Plasma secretin and cholecystokinin (CCK) concentrations of diabetic rats were significantly increased compared to those of normal rats. The altered pancreatic exocrine function was abolished by the exogenous insulin administration. The exogenous insulin also restored the increased plasma secretin and CCK concentrations. From the above results, it is suggested that, in streptozotocin-induced diabetic rats, anticoordinated changes in pancreatic enzymes secretion as well as pancreatic enzymes content are attributable to insulin deficiency and that the insulin deficiency is responsible for the increased plasma concentrations of both secretin and CCK. However, it is not clear whether the elevated plasma secretin and CCK concentrations played a direct role in changes of pancreatic exocrine function.

Regional distribution and relative frequency of the gastrin, secretin and pancreatic polypeptide-immunoreactive cells in the gastrointestinal tract of the fetus of Korean native goat (한국재래산양 태자의 위장관에 있어서 gastrin, secretin 및 pancreatic polypeptide 면역반응세포의 분포 및 출현빈도에 관한 연구)

  • Lee, Hyeung-sik;Ku, Sae-kwang;Lee, Jae-hyun
    • Korean Journal of Veterinary Research
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    • v.39 no.1
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    • pp.12-19
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    • 1999
  • The regional distributions and relative frequencies of the gastrin, secretin and pancreatic polypeptide(PP)-immunoreactive cells in the gastrointestinal tract of the fetus(180 days of gestation) of Korean native goat were studied with immunohistochemical(ABC) methods. Gastrin-immunoreactive cells were detected in fundus, pylorus and duodenum and these cells were most predominant in pylorus. Secretin-immunoreactive cells were observed in pylorus, duodenum and ileum. PP-immunoreactive cells were restricted to fundus. These immunoreactive cells were situated in surface epithelium and mucosal gland regions. The regional distribution and relative frequency of PP-immunoreactive cells was somewhat different to the adult Korean native goat. Immunoreactive cells in the surface epithelial regions were open typed cells which were spindle shaped cells but closed typed cells which were round or/to spherical shaped cells were observed in the mucosal gland regions.

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Effects of Intragastric Hypertonic Solution on Pancreatic Exocrine Secretion (고장수액의 위내 주입으로 인한 취외분비의 변동)

  • Cho, T.S.;Kim, W.J.;Hong, S.S.
    • The Korean Journal of Pharmacology
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    • v.13 no.1
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    • pp.29-33
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    • 1977
  • Effects of 50% glucose solution on pancreatic exocrine function were studied in rat, rabbit and cat. The alterations during the resting state, the continuous intravenous infusion of secretin and the infusion of secretin with CCK-PZ were determined. 1) No change of pancreatic secretion in rat was observed by intragastric administration of the hypertonic glucose solution. 2) Intragastric administration of the hypertonic glucose solution in rabbit produced the inhibitory effect on pancreatic secretion during secretion infusion. 3) While secretin with CCK-PZ were infused continuously, intragastric administration of the hypertonic glucose solution revealed the marked inhibitory effect on pancrcreatic secretion in cat. Oral administration of the hypertonic glucose solution produced no significant inhibition in the resting gland but markedly depressed the pancreatic flow and enzyme concentration in the secretin or CCK-PZ stimulated gland. It is felt that the inhibitory response of exocrine pancreas induced by intragastric hytertonic glucose solution is resulted in interaction between secretory hormone and gastric mucosal factor possibly enteroglucagon.

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