• 제목, 요약, 키워드: Satellite cell activity

검색결과 7건 처리시간 0.021초

Factors Influencing Satellite Cell Activity during Skeletal Muscle Development in Avian and Mammalian Species

  • Nierobisz, Lidia S;Mozdziak, Paul E
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.21 no.3
    • /
    • pp.456-464
    • /
    • 2008
  • Avian and mammalian skeletal muscles exhibit a remarkable ability to adjust to physiological stressors induced by growth, exercise, injury and disease. The process of muscle recovery following injury and myonuclear accretion during growth is attributed to a small population of satellite cells located beneath the basal lamina of the myofiber. Several metabolic factors contribute to the activation of satellite cells in response to stress mediated by illness, injury or aging. This review will describe the regenerative properties of satellite cells, the processes of satellite cell activation and highlight the potential role of satellite cells in skeletal muscle growth, tissue engineering and meat production.

Influence of co-culturing muscle satellite cells with preadipocytes on the differentiation of adipocytes and muscle cells isolated from Korean native cattle

  • Choi, Chang Weon
    • 농업과학연구
    • /
    • v.45 no.4
    • /
    • pp.715-723
    • /
    • 2018
  • The present study was done to investigate the effect of co-culturing muscle satellite cells (MSCs) and intramuscular preadipocytes (IPs) on the differentiation of adipocytes and muscle cells isolated from Korean native cattle. MSCs and IPs were single-cultured in 10% fetal bovine serum/Dulbecco's modified Eagles medium (FBS/DMEM) for 48 h followed by culturing in 5% FBS/DMEM as the growth media. Then, the growth media was replaced by differentiation media composed of 2% FBS/DMEM without any additives for the single- or co-culture of muscle cells and intramuscular adipocytes to induce the differentiation of both cell types. Cell differentiation was measured by morphological investigation and cytosolic enzyme analysis of glycerol-3-phosphate dehydrogenase (GPDH) for the adipocytes and creatine kinase (CK) for the muscle cells. In the morphological test, the presence of muscle cells did not stimulate adipocyte differentiation showing more differentiation of the adipocytes in the single-culture compared to the co-culture condition. However, the differentiation of muscle cells was promoted by adipocytes in the co-culture. The results of the enzymatic analysis were highly associated with the morphological results with a statistically higher GPDH activity (p < 0.05) appearing in the single-culture than in the co-culture, whereas the opposite was true for the CK activity of the muscle cells (p < 0.05). By manipulating in vivo the milieu using a co-culture, we could detect the difference in the rate of cell differentiation and suggest that a co-culture system is a more reliable and precise technique compared to a single-culture. Further studies on various co-culture trials including supplementation of differentiating substances, gene expression analysis, etc. should be done to obtain practical and fundamental data.

Construction of Korean Space Weather Prediction Center: Space radiation effect

  • Lee, Jae-Jin;Cho, Kyung-Suk;Hwang, Jung-A;Kwak, Young-Sil;Kim, Khan-Hyuk;Bong, Su-Chan;Kim, Yeon-Han;Park, Young-Deuk;Choi, Seong-Hwan
    • 한국우주과학회:학술대회논문집(한국우주과학회보)
    • /
    • /
    • pp.33.3-34
    • /
    • 2008
  • As an activity of building Korean Space Weather Prediction Center (KSWPC), we has studied of radiation effect on the spacecraft components. High energy charged particles trapped by geomagnetic field in the region named Van Allen Belt can move to low altitude along magnetic field and threaten even low altitude spacecraft. Space Radiation can cause equipment failures and on occasions can even destroy operations of satellites in orbit. Sun sensors aboard Science and Technology Satellite (STSAT-1) was designed to detect sun light with silicon solar cells which performance was degraded during satellite operation. In this study, we try to identify which particle contribute to the solar cell degradation with ground based radiation facilities. We measured the short circuit current after bombarding electrons and protons on the solar cells same as STSAT-1 sun sensors. Also we estimated particle flux on the STSAT-1 orbit with analyzing NOAA POES particle data. Our result clearly shows STSAT-1 solar cell degradation was caused by energetic protons which energy is about 700 keV to 1.5 MeV. Our result can be applied to estimate solar cell conditions of other satellites.

  • PDF

Effect of stocker management program on beef cattle skeletal muscle growth characteristics, satellite cell activity, and paracrine signaling impact on preadipocyte differentiation

  • Vaughn, Mathew A.;Lancaster, Phillip A.;Roden, Kelly C.;Sharman, Evin D.;Krehbiel, Clinton R.;Horn, Gerald W.;Starkey, Jessica D.
    • Journal of Animal Science and Technology
    • /
    • v.61 no.5
    • /
    • pp.260-271
    • /
    • 2019
  • The objective of this study was to determine the effect of different stocker management programs on skeletal muscle development and growth characteristics, satellite cell (SC) activity in growing-finishing beef cattle as well as the effects of SC-conditioned media on preadipocyte gene expression and differentiation. Fall-weaned Angus steers (n = 76; $258{\pm}28kg$) were randomly assigned to 1 of 4 stocker production systems: 1) grazing dormant native range (NR) supplemented with a 40% CP cottonseed meal-based supplement ($1.02kg{\cdot}steer^{-1}{\cdot}d^{-1}$) followed by long-season summer grazing (CON, 0.46 kg/d); 2) grazing dormant NR supplemented with a ground corn and soybean meal-based supplement fed at 1% of BW followed by short-season summer grazing (CORN, 0.61 kg/d); 3) grazing winter wheat pasture (WP) at high stocking density (3.21 steers/ha) to achieve a moderate rate of gain (LGWP, 0.83 kg/d); and 4) grazing winter WP at low stocking density (0.99 steers/ha) to achieve a high rate of gain (HGWP, 1.29 kg/d). At the end of the stocker (intermediate harvest, IH) and finishing (final harvest, FH) phases, 4 steers / treatment were harvested and longissimus muscles (LM) sampled for cryohistological immunofluorescence analysis and SC culture assays. At IH, WP steers had greater LM fiber cross-sectional area than NR steers; however, at FH, the opposite was observed (p < 0.0001). At IH, CORN steers had the lowest Myf-5+:Pax7+ SC density (p = 0.020), while LGWP steers had the most Pax7+ SC (p = 0.043). At FH, CON steers had the highest LM capillary density (p = 0.003) and their cultured SC differentiated more readily than all other treatments (p = 0.017). At FH, Pax7 mRNA was more abundant in 14 d-old SC cultures from HGWP cattle (p = 0.03). Preadipocytes exposed to culture media from proliferating SC cultures from WP cattle isolated at FH had more $PPAR{\gamma}$ (p = 0.037) and less FABP4 (p = 0.030) mRNA expression compared with NR cattle. These data suggest that different stocker management strategies can impact skeletal muscle growth, SC function, and potentially impact marbling development in growing-finishing beef cattle.

한반도에서 발생하였던 집중호우 시 적외 및 수증기 영상의 특성 (Characteristics of Infrared and Water Vapor Imagery for the Heavy Rainfall Occurred in the Korean Peninsula)

  • 성민규;서명석
    • 대한원격탐사학회지
    • /
    • v.30 no.4
    • /
    • pp.465-480
    • /
    • 2014
  • 본 연구에서는 최근 발생한 집중호우 사례들 중 예보가 어려워 피해가 컸던 두 사례(2010년 9월 21일, 2011년 8월 9일)에 대해 적외영상과 수증기영상의 시 공간적인 변화 특성을 분석하였다. 두 사례에서 한반도지역에 집중호우를 유발한 대류 세포들은 적외영상에서 하층운이 광범위하게 분포하고 수증기 영상에서는 명역과 암역의 경계(boundary)에서 생성되는 특징을 보였다. 또한 대류 세포들의 이동속도 차에 의한 총 5번의 병합과정 중 4번의 병합과정에서 대류 세포들의 병합 후 대류 세포는 더욱 발달되었으며 강수 강도도 급격하게 강화되었다. 대류시스템에서의 강우강도 변화는 휘도온도의 평균보다 최소 휘도온도의 시간적 변화와 밀접하게 관련된 것으로 판단되며 대류 세포들의 병합도 집중호우의 강도 변화에 영향을 주는 주요 인자로 생각된다. 대류 세포들의 병합은 영상동화를 통해 어느 정도 예측이 가능하지만 대류 세포의 탐지는 적외 및 수증기 영상 모두에서 일정 강도 이상 발달한 상태에서만 탐지가 가능하였다.

근육세포 분화에 대한 TGF-β1과 OP-1의 억제 효과 (The Inhibitory Effect of TGF-β1 and OP-1 onto the Myogenic Differentiation)

  • 김병국;정성수
    • Journal of Oral Medicine and Pain
    • /
    • v.26 no.1
    • /
    • pp.39-50
    • /
    • 2001
  • In order to investigate the effect of Transforming growth factor ${\beta}1$(below TGF-${\beta}1$) and osteogenic protein-1(below Op-1) onto the myogenic differentiation, C2C12 satellite myoblastic cell line was cultured and treated with both growth factors. At first morphological changes with microscopical examination were examined, and isolated total RNA to analyse mRNA expression of bone marker proteins, muscle regulatory proteins, TGF-${\beta}$ receptor and their ligands by Northern blot analysis. And cellular proliferative inducibility of both growth factors was also tested to C2C12 cells. Incubating the cell with $5ng/m{\ell}$ of TGF-${\beta}1$ until 4 days almost inhibited multinucleated myotube formation expressing muscular regulatory proteins, and induced decreasing Id proteins. However, no osteoblastic phenotypes was induced by TGF-${\beta}1$ in C2C12 cells. The mRNA expression of TGF-${\beta}$ receptors with TGF-${\beta}1$ was conversed after 48 hours cultured. Type I TGF-${\beta}$ receptor was seemed to play a role in negative signalling for inhibition of myogenic differentiation. OP-1 dose dependently induced ALP activity, osteopontine production and bone sialoprotein production at concentrations above $100ng/m{\ell}$ and osteocalcin production at concentrations above $300ng/m{\ell}$. The concentration of OP-1 required to induce these osteoblastic phenotypes was the same as that required to almost completely inhibit myotube formation. Incubation with above $100ng/m{\ell}$ OP-1 suppressed the expression of mRNA for muscular egulatory proteins from 2 days after incubation. Expression of Id-1, 2, 3 mRNA were stimulated by OP-1 at concentration above $300ng/m{\ell}$. When C2C12 cells were treated with both growth factors, TGF-${\beta}1$ potentiated the inhibitory effect of OP-1 on myotube formation and expression of mRNA for myogenin at 12 days. And TGF-${\beta}1$ reduced osteocalcin and bone sialoprotein production induced by OP-1 at 12 days in C2C12 cells. Both growth factor had no mitogenic effect. These results indicate that OP-1 converts the differentiation pathway of C2C12 myoblasts into that of osteoblastic lineage cells and it's not heritable, but TGF-${\beta}1$ does not and has reversible inhibitory activity on the myogenic differentiation. TGF-${\beta}1$ and OP-1 play a role in myogenic differentiation via different mechanism between them.

  • PDF

제브라피쉬 근육성장에서의 carnosic acid의 효과 (Effects of Carnosic Acid on Muscle Growth in Zebrafish (Danio rerio))

  • 김정환;진덕희;김영대;진형주
    • 한국어류학회지
    • /
    • v.26 no.3
    • /
    • pp.171-178
    • /
    • 2014
  • 로즈마리의 주요 성분인 carnosic acid는 carnosol, rosmarinic acid, ursolic acid 등과 같은 폴리페놀의 한 성분으로 다양한 생리활성 기능이 보고되어 있다. 본 연구에서는 로즈마리 유래 폴리페놀인 carnosic acid가 제브라피쉬 근육성장에 미치는 영향을 근육 내 주사와 사료를 통해서 확인해 보았다. 근육 내 주사 실험을 통해서 CA는 제브라 피쉬의 근육 내 단백질 함량을 증가시키고 중성지방의 함량을 감소시켰다. 또한 조직학적 분석 결과 근섬유의 평균 면적이 커지는 근섬유의 과비대 효과를 나타내었다. 사료 실험 결과 근육 내 단백질 및 중성지방의 함량에는 영향을 미치지 않았으며 조직학적 분석 결과 근육 내 주사 실험에서와 마찬가지로 근 섬유의 과비대를 유도하였다.