• Title, Summary, Keyword: Saponin

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Effect of ginseng Saponin on the fatty acid composition of the lipids of Rhodotorula glutinis. (인삼 Saponin이 Rhodotorula glutinis의 지방산 조성에 미치는 영향)

  • 이시경;주현규
    • Microbiology and Biotechnology Letters
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    • v.13 no.2
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    • pp.109-114
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    • 1985
  • This study was attempted to observe the effect of crude ginseng saponin on the cell growth, lipid contents of Rhodotolula glutinis cells and fatty acid composition of the lipids from the yeast. The results obtained were as follows; The weight of dry yeast cells was increased by the addition of ginseng saponin and was noticeably increased in the treatment containing 10$^{-2}$ % ginseng saponin. With the increase of ginseng saponin concentration, lipids contents in Rhodotorula glutinis were increased but it's contents were decreased at the concentration of 10$^{-1}$ % ginseng saponin. The crude lipids of Rhodotorula glutinis were identified to be composed of palmitic, stearic, oleic, linoleic acid. Un-saturated fatty acid contents were greater than those of saturated fatty acids. Fatty acid contents were increased in the order of oleic, linoleic, palmitic and stearic acid, but palmitic acid was increased more than linoleic acid in the lipids of cells obtained at the higher than 10$^{-2}$ % ginseng saponin. As the ginseng saponin concentration was increased fatty acid contents were also increased, while they were considerably decreased in case of the addition of 10$^{-1}$ % ginseng saponin concentration.

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Effect of Ginseng Saponin on the Proliferation and Viability of Murine Thymocyte, in vitro (생쥐 흉선세포의 증식과 생존력에 미치는 인삼 사포닌의 영향)

  • 최선경;정노팔
    • Journal of Ginseng Research
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    • v.10 no.2
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    • pp.133-140
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    • 1986
  • Ginseng saponin had an effect on the proliferation and viability of cultured murine thymocytes. When the thymocytes were cultured in various concentrations of ginseng saponin, the number of thymocytes increased at $10^{-5}$% ginseng saponin but decreased at $10^{-5}$%. There was little change in the number of thymocytes when cultured in IL 2(Interleukin 2), a factor known for its influence on the proliferation and maturation of thymocytes. When the thymocytes were cultured in various concentrations of IL 2 with $10^{-5}$% ginseng saponin, the number of total cells increased at 1.5% or 3% IL 2 when cultured for 9 hours, or at 6% IL 2 for 12, 24, or 48 hours. But there was little change in the number of viable cells. In vitro, ginseng saponin had an effect on the activity of ADA(Adenosine Deaminase), an enzyme known to affect the production of IL 2. There was a 25% increase in the activity of ADA in the presence of $10^{-5}$% ginseng saponin.

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Effects of Saponins on the Osmotic Behavior of Multilamellar Liposomes

  • Yu, Byung-Sul;Chung, Hyun-Ho;Kim, Aeri
    • Archives of Pharmacal Research
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    • v.7 no.1
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    • pp.17-22
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    • 1984
  • Effects of total ginseng saponin, 20-S-protopanaxadiol saponin, 20-S-protopanaxatriol saponin and playcodon saponin on the osmotic behavior of liposomes were investigated by optical measurement. These saponins showed different activities on liposomal membrane, and cholesterol in liposomes was an important factor to this variation of saponin activities.

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Effect of Crude Saponin Fraction from Korean Red Ginseng on Physiological Events of Ovariectomized Rat (난소절제 흰쥐의 생리적 장애에 미치는 고려홍삼 조사포닌 분획의 영향)

  • 곽이성;위재준;황석연;경종수;김시관
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.2
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    • pp.288-293
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    • 2000
  • This study was carried out to investigate the effect of crude saponin fracton from Korean red ginseng on physiological events of ovariectomized rats. The saponin fractions was prepared by Diaion HP-20 adsorption chromatography and spirit. Total 50 rats were divided into 5 groups : normal control (NC), sham-ovariectomized (SO), ovariectomized (OR), ovariectomized and saponin treated (OS), and normal control treated with saponin (NS). Saponin was intraperitonally administered for 8 weeks. Ovariectomy was carried out at 1 st and 2nd weeks of experiment. Right-side ovary of rat was removed at 1st week, the otehr was done at 2nd week. The body weights fo ovariectomized rats showed no significant changes but that of NS group showed significant increase (p<0.05) compared with NC group. Ovariectomy of rats reduced the weights of femur by 6 to 8% compared with that of NC group. In addition, femur weight of NS group was 5 to 6% higher than that of NC. Uterus weight showed no significant differences by saponin treatment or ovariectomy. Serum testosterone level of ovariectomized rats increased by 60 times compared with that of NC. However, administration of crude saponin to ovariectomized rat attenuated testosterone level to almost that of NC. These results suggest that Korean red ginseng saponin attenuates physiological disorders induced by malfunction of ovary.

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Effect of Saponin on Sodium-Potassium activated ATPase in Rabbit Red Cell Membrane (Saponin이 토끼 적혈구막의 $Na^{+}-K^{+}-ATPase$의 활성도에 미치는 영향)

  • Kang, Byoung-Nam;Koh, Il-Sup
    • The Korean journal of physiology & pharmacology
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    • v.8 no.1
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    • pp.67-76
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    • 1974
  • The effect of saponin on the sodium plus potassium activated ATPase activity was studied in the rabbit red cell ghosts and the experiments were also designed to determine the mechanism of action of saponin on the APTase activity. The following results were observed. 1. The ATPase activity of rabbit red cell ghosts is inhibited by low concentration of saponin but increased by high concentration. The activating effect of saponin on the $Na^{+}-K^{+}-ATPase$ activity is inhibited by ouabain but the stimulation of the $Mg^{++}-ATPase$ by high concentration of saponin is not inhibited by ouabain. 2. The activity ratio of $Na^{+}-K^{+}-ATPase$ by high concentration of saponin is decreased by raising the potassium concentration, and is increased by raising the sodium concentration. 3. The ATPase activity is increased by small amounts of calcium but inhibited by larger amounts. The activity ratio of the enzyme by saponin is decreased by raising the calcium concertration 4. The action on the ATPase activity was not related to the amino group of lysine, the hydroxyl group of threonine, the imidazole group of histidine, or the carboxyl group of aspartic acid. 5. The action of saponin on the ATPase activity is due to sulfhydryl group of the enzyme of $Na^{+}-K^{+}-ATPase$.

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Studies on the Extracting Methods of Ginseng Extract and Saponins in Panax Ginseng (유출조건에 따른 인삼중의 Ginseng Extract와 Saponin 합량변화에 관한 연구)

  • 주현규;조규성
    • Journal of Ginseng Research
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    • v.3 no.1
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    • pp.40-53
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    • 1979
  • In order to investigate the optimal conditions which affects to extraction of ginseng extract and saponin in ginseng extract, experiment was carried out varing with ethanol percentage, extraction time, temperature, sol$.$vent and Plant Parts. The results art as follows: 1. The amounts of ginseng saponin was increased according to increanation of ethanol Percentage while the amounts of ginseng extract was decreased. 2. The amounts of ginseng extract was increased as the prolongation of extraction time, on the ether hand, ginseng saponin contents increased lentil 40hr. and decreased after that. 3. By the raise of extract temperature, both of the amounts of ginseng saponin and ginseng extract was increased two times and four times. respectively. 4. The total amounts ginseng extract was obtained 22.86u when the water used as the extraction solvent, 11.28% on ethanol and 11.04U on methanol, in the order. and the saponin contents gained when the extraction solvents of water, methanol and ethanol 7.47%, 12.36% and 12.77%, respectively. 5. It showed 9.23% of ginseng extract in epidermis and 8.4% of ginseng saponin in tail Part of raw ginseng and in the case of dried ginseng, ginseng extract and saponin showed the most amounts in epidermis of 18.28% and 19.35%, respectively. 6. The ratio of panaxadiol and panaxatriol contents of ginseng saponin was almost same when it was extracted varing with ethanol percentage and extraction time (duration), and the more alcohol percentage and the longer extraction time increased, the more fractional content of ginseng saponin was extracted.

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Effect of Ginseng Saponin, Gypsophila Saponin, and Detergents on Volume Changes and Fragility of Red Blood Cells (인삼(人蔘) Saponin, 은시호(銀柴胡) Saponin 및 계면활성제(界面活性劑)가 적혈구(赤血球)의 용적변화(容積變化) 및 Fragility에 미치는 영향(影響))

  • Lee, Shin-Woong;Lee, Jeung-Soo;Lee, Soo-Kun;Ok, Chang-Kun;Kim, Young-Hie
    • YAKHAK HOEJI
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    • v.33 no.1
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    • pp.15-19
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    • 1989
  • The effects of Gypsophila saponin, sodium dodecylsulfate (SDS) and Triton X-100 on volume changes and fragility of red blood cells were compared to ginseng saponin to elucidate whether there are any difference in their action on membrane lipid. Cell volume was decreased to about 38% in 1M NaCl and increased to about 20% in 1/10M NaCl. Hematocrit value was decreased by Gypsophila saponin, SDS, and Triton X-100 which caused hemolysis in isotonic NaCl solution. These detergents also inhibited increase of cell volume and accelerated hemolysis in hypotonic solution. However, ginseng saponin did not influence to osmotic volume changes and hemolysis of red blood cells. These results suggest that the disruptive effect of plant saponin on membrane barriers induced by removing membrane lipid is different from their source and ginseng saponin has very low affinity to membrane lipid.

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Effects of Total Saponin and Oil Fraction of Polygala tenuifolia on Serum Cholesterol Level in Rabbits (원지 Saponin 및 원지유가 가토의 혈청 Cholesterol 함량에 미치는 영향)

  • 우원식;이관용
    • YAKHAK HOEJI
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    • v.6 no.1
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    • pp.8-10
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    • 1962
  • Total saponin and oil fraction of Polygala tenuifolia were tested for their anticholesterolemic properties by a "Three day fasting method" on rabbits. It has been reported that saponin have the anticholesterolemic activity, however this activity is not general property of all kinds of saponin. As shown in Table, Polygala saponin has not significant anticholesterolemic activity on rabbits. On administering oil fraction of Radix Polygala, serum cholesterol level in rabbits increased to 224.7% while the control group 43.2%.oup 43.2%.

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Effect of Ginseng Saponin Fraction on Absorption of Cholesterol and Serum Lipid Components (인삼 사포닌 분획이 Cholesterol의 흡수 및 혈청지질 성분에 미치는 영향)

  • 정인실;조영동
    • Journal of Ginseng Research
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    • v.9 no.2
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    • pp.232-239
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    • 1985
  • The effects of total saponin, triol and diol saponin, and prosapogenin of ginseng on the absorption of cholesterol and the component of serum lipids was observed as a function of time using isotopic method. The following results were obtained. 1) Labelled cholesterol absorption was observed to be enhanced after adminstrations of total saponin, triol saponin, and. prosapogenin of ginseng to rat (Sprague Dawley). 2) Decreases in the amount of serum lipid were observed after administration of total saponin and prosapogenin of ginseng.

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Change of Ginsenosides and Free Sugars in Seeds During Stratification and Seedling During Early Growth Stage of Panax ginseng (인삼의 종자개갑시와 묘생육초기의 Ginsenosides 및 유리당의 변화)

  • 박귀희;이미경;박훈
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.3
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    • pp.286-292
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    • 1986
  • For the elucidation of saponin synthesis during ontogeny changes of ginsenosides and free sugars in seeds during stratification and seedlings in early growth stage were investigated with high performance liquid chrom-atography. Embryo plus endosperm at 40-day stratification showed 80% decrease of total saponin, disappear-ance of Rc, Rb$_2$ and Rb$_1$ and appearance of Rg$_3$ (probable) and 20-Glc-Rf (probable). Leaf ginsenoside F$_3$ was found not in fruit plup but seed and decreased during stratification. Both decomposition and synthesis of saponin seemed to occure during stratification. Ginsenosides in endosperm and embryo might be originated from fruit pulp by penetration. In seedling saponin appeared first in shoot and in root about one month later. Ginsenoside Rc, Rb$_2$, Rb$_1$ appeared in root at the last investigation (June 30) indicating normal saponin synthetic capacity of root. Saponin synthetic rate was twice in leaf than in root. Leaf ginsenoside F$_3$ was found in seedling root. Root saponin Rg$_3$ and 20-Glc-Rf were found in leaf and stem in seedling and decreased with growth suggesting that rate saponin is not such in certain growth stage. Total saponin content was negatively correlated with PT/PD in seeds and arial parts of seedling due to greater change of PD. than PT. Seed at 70days stratification showed high sucrose content. In seedling glucose was main sugar in stem all the while and sucrose in root at early stage while glucose, fructose and sucrose were found in leaf.

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