• Title, Summary, Keyword: Saponin

Search Result 1,290, Processing Time 0.046 seconds

Effect of Ginseng Saponin on the Activity, Phosphorylation, $[^3H]$Ouabain Binding of Purified$Na^+$ $K^+$-ATPase Isolated from the Outer Medulla of Sheep Kidney (인삼 Saponin이 양신장에서 정제한 $Na^+$ $K^+$-ATPase의 활성, 인산화 및 $[^3H]$Ouabain결합에 미치는 영향)

  • 이신웅;이정수;진갑덕
    • YAKHAK HOEJI
    • /
    • v.29 no.2
    • /
    • pp.76-89
    • /
    • 1985
  • The effects of ginseng saponin on the activity, phosphorylation, [$^{3}$H] ouabain binding and light scattering (disruption) of purified $Na^{+}$ ,$K^{+}$ -ATPase isolated from the outer medulla of sheep kidney were compared to those of gypsophila saponin, sodium dodecylsulfate (SDS), and Triton X-100 on the same parameters. $Na^{+}$ , $K^{+}$ -ATPase activity, phosphorylation, and [$^{3}H$] ouabain binding were inhibited by ginseng saponin (triol>total>diol), SDS, or Triton X-100, but increased by gypsophila saponin. Low doses of ginseng saponin (3.mu.g saponin/.mu.g protein) decreased phosphorylation sites and ouabain binding site concentration (Bmax) without any change of turnover number and affinity for ouabain binding which were decreased by high dose of ginseng saponin (over 10.mu.g saponin/.mu.g protein), SDS or Triton X-100. On the other hand, gypsophila saponin increased the affinity without any change of Bmax for ouabain binding. Inhibition of $Na^{+}$ ,$K^{+}$ -ATPase activity by ginseng saponin and SDS or Triton X-100 appeared before and after decrease in light scattering, respectively. These data suggest that ginseng saponins (total, diol, triol saponin) inhibit $Na^{+}$ , $K^{+}$ -ATPase activity by specific direct and general detergent action at low and high concentrations, respectively, and this inhibitory action of ginseng sapornin to $Na^{+}$ , $K^{+}$ -ATPase is not general action of all saponins.

  • PDF

Variation of Saponin Content in Korean Native Soybean Landraces Reintroduced from USA to Korea (미국으로부터 재도입된 한국 원산 콩 재래종의 Saponin 함량 변이)

  • Jang, Eun-Kyu;Piao, Xiangmin;Hwang, Tae-Young;Kim, Sun-Lim;Chung, Gyu-Hwa;Tsukamoto, Chigen;Choi, Yu-Mi;Lee, Myung-Chul;Lee, Jeong-Ran;Kim, Hong-Sig
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.57 no.3
    • /
    • pp.286-295
    • /
    • 2012
  • This study was carried out to assess the variation of saponin contents in 293 Korean native soybean landraces reintroduced from USA to Korea in 2007. Group A saponin content ranged from 149.8 ${\mu}g\;g^{-1}$ to 1279.0 ${\mu}g\;g^{-1}$ with an average of 484.9 ${\mu}g\;g^{-1}$. Group B saponin content ranged from 2160.1 ${\mu}g\;g^{-1}$ to 7868.6 ${\mu}g\;g^{-1}$ with an average of 3670.0 ${\mu}g\;g^{-1}$. Total saponin content including both group A and B contents ranged from 2502.8 to 8764.0 ${\mu}g\;g^{-1}$ with an average of 4154.9 ${\mu}g\;g^{-1}$. Five promising landraces for use as breeding materials that showed higher than any other landraces in group B and total saponin content were IT226841, IT226761, IT226841, IT226828 and IT228534. IT226841 showed the highest saponin content with group B content of 7868.5 ${\mu}g\;g^{-1}$ and total saponin content of 8764.0 ${\mu}g\;g^{-1}$ among landraces. In the relationship among each components, group A saponin content showed a positive correlation with group B saponin content (r=0.3708) and total saponin content (r=0.5119). Group B saponin content showed a very high positive correlation (r=0.9876) with total saponin content. When landraces were compared for the total saponin content based on collected location, landraces from North Korea showed the highest, Gyeonggi-do showed second highest content, followed by Chungcheongbuk-do, Gangwon-do, Gyongsangbuk-do, Chungcheongnam-do, Gyongsangnam-do, Jeollabuk-do and Jeollanam-do. Landraces were also grouped according to seed size and seed coat color. Small seed group was higher than medium and large seed groups that showed no significant difference in total saponin content. Seed coat colors showed no significant difference in total saponin content.

The Effect of Ginseng Saprophagous Fungi on Change of Crude Saponin Components (인삼(人蔘) 부패(腐敗)곰팡이가 인삼(人蔘) Saponin 성분변화(成分變化)에 미치는 영향(影響))

  • Jung, Dong-Kon;Park, Kil-Dong;Ha, Seung-Soo;Joo, Hyun-Kyu
    • Korean Journal of Food Science and Technology
    • /
    • v.21 no.3
    • /
    • pp.345-350
    • /
    • 1989
  • Saprophagous fungi which were isolated from ginseng products were investigated the change of mycellial weight, saponin pattern and saponin contents according to culture periods at different of saponin concentration. Aspergillus sp. showed the greatest mycellial weight in 9 days at 0.3% saponin concentration as well as Penicillium specise A and B. Mycellial weight of all Saprophagous fungi was decresed than control group at 1.0% concentration of crude saponin. Saponin pattern were changed in 6th days of culture by Aspergillus sp. at 0.3% and deteriorated diol ginsenoside respectively. The amount of diol saponins was decreased all the duration of culture by Aspergillus sp. and Penicillium sp. B. whereas Pencillium sp. A was not any change. The amont of saponin in the fresh ginseng and white ginseng medium was decreased gradually according to culture periods by the saprophagous fungi.

  • PDF

The Effect of Ginseng Saponin Fractions on Humoral Immunity of Mouse (생쥐의 체액성 면역에 미치는 인삼사포닌 분획물들의 영향)

  • Park, Han-U;Kim, Se-Chang;Jeong, No-Pal
    • Journal of Ginseng Research
    • /
    • v.12 no.1
    • /
    • pp.63-67
    • /
    • 1988
  • To investigate the effect of ginseng saponin fractions (total saponin, diol saponin and triol saponin) on the antibody production and on the recovery of immunosuppression in mouse, chick ${\gamma}$-globulin was used as immunogen and CY(cyclophosphamide) as immunosuppressive drug. The effect of ginseng saponin fractions on the production of total serum protein was investigated also. Circulating antibody was measured with ELISA method. Total saponin, dial saponin and triol saponin resulted 4 times higher titer values compared to control group in the production of antibody but resulted no effect on the recovery of immunosuppression induced by CY. From the above results ginseng saponin fractions are believed to effect on intact immune system and to promote antibody production by helping the cooperations among lymphocytes or the growth of lymphocytes. And the increase of total serum protein has no direct relations with the increase of circulatory antibody.

  • PDF

Effects of Ginseng Saponin Fraction and Cyclophosphamide on the Tumoricidal Activity of Mouse Macrophage and the Antitumor Effect (생쥐의 대식세포 종양치사활성과 항암효과에 미치는 인삼 Saponin 분획물과 Cyclophosphamide의 영향)

  • Jeon, Hye-Gyeong;Kim, Se-Chang;Jeong, No-Pal
    • Journal of Ginseng Research
    • /
    • v.15 no.2
    • /
    • pp.99-105
    • /
    • 1991
  • This experiment was performed to investigate the effects of ginseng saponin fraction and cyclophosphamide (CY) on the tumor development, the antitumor effect and the tumoricidal activity of mouse macrophage. When mice were treated with saponin or CY following inoculation with Sarcoma 180, tumor development was inhibited and survival ratio increased, and a combination of both treatments further inhibited the tumor development. Tumoricidal activity of macrophage was effectively increased at 10-7% concentration of CY and it was further increased when macrophage was cotreated with saponin and CY. Tumoricidal activity of macrophage was greatest at the third day after inoculating tumor cell. Both saponin and CY increased the chemiluminescence of macrophage, but CY had no effect on releasing TNF, unlike saponin.

  • PDF

A Study on the Effect of Ginseng Saponin on Rat Intestinal Mucosal $Na^+,K^+$-ATPase (인삼 사포닌이 백서 장점막 $Na^+,K^+$-ATPase에 미치는 영향에 관한 연구)

  • 조윤성;김낙두;권용화
    • YAKHAK HOEJI
    • /
    • v.22 no.3
    • /
    • pp.120-127
    • /
    • 1978
  • We have studied the effect of ouabain, tool ginseng saponin, panax saponin C (protopanaxatriol derivative) and ginsenoside $Rb_{1}$ (protopanaxadiol derivative) on $Na^+,K^+$-ATPase and $Mg^{++}$-ATPase activities were determined by the method of Robinson and ATPase activities were determined by the method of King. The $Na^+,K^+$-ATPase activities were inhibitied by 90.1% and 51.1% respectively at the concentration of $10^{-3}M$ and $10^{-4}M$ ouabain. The results are consistent with those of Robinson. The $Na^+,K^+$-ATPase activities were increased by 14.3% and 10.0% respectively at the concentration of $10^{-4}$g/ml and $10^{-5}$g/ml total ginseng saponin. Panax saponin C obtained by the method of Han and ginsenoside $Rb_{1}$ obtained by the method of Shibata were used. The $Na^+,K^+$-ATPase activities were increased in the presence of panax saponin C and the increased activity with panax saponin C was greater than that with total ginseng saponin. On the other hand ginsenoside $Rb_{1}$ showed an inhibitory effect on $Na^+,K^+$-ATPase. Total ginseng saponin, panax saponin C and ginsenoside $Rb_{1}$ had no effect on $Mg^{++}$-ATPase. Therefore, it may be concluded that total ginseng saponin has dual effects on microsomal $Na^+,K^+$-ATPase, that is, panax saponin C exhibits stimulatory action, whereas ginsenoside $Rb_{1}$ shows inhibitory action.

  • PDF

Saponins of Korean Ginseng C.A. Meyer(PartII)-The saponins of the ground part of ginseng- (한국인삼(韓國人蓼)의 Saponin에 관(關)한 연구(硏究)제2보(第二報)-인삼지상부(人蔘地上部) Saponin함량(含量)에 관(關)하여-)

  • Cho, Sung-Hwan
    • Applied Biological Chemistry
    • /
    • v.20 no.1
    • /
    • pp.142-146
    • /
    • 1977
  • For the purpose of utilizing the saponins of the ground part of Panax ginseng, the studies on saponins isolated from the leaves, stems, and flowers of ginseng were Performed. The results were summarized as follows: 1. The crude saponin contents of ginseng loaves, stems, and flowers 12.8%, 1.6% and 6.9% respectively. 2. Infra-red spectrum of ginseng leaf saponin was identical with that of ginseng root.

  • PDF

Effect of Ginseng Saponin on The Growth of Heat-Stressed Yeast Saccharomyces rouxii - Protective Effect of Saponin on The Heat Denaturation of The Amylase Produced by Saccharomyces rouxii- (열처리된 효모의 생육에 미치는 인삼 Saponin의 영향 -Saccharomyces rouxii가 생산한 Amylase의 열변성에 대한 보호작용-)

  • 오영숙;이갑랑
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.19 no.6
    • /
    • pp.591-595
    • /
    • 1990
  • A promoting effect of ginseng saponin on the growth recovery rate of Saccharomyces rouxii which was treated by heat was confirmed in previous report(22). In order to deduce the promoting effect on the growth recovery of the heat stressed yeast, the effect of ginseng saponin on the activity and the heat stability of the amylase produced by Sacchirromyces rouxii were observed. The amylase showed the highst activity at 0.01% of saponin. At this concentration, the activity increased about 23% compared to the control. Furthermore, the ginseng saponin showed a protective effect against thermal inactivation of the amylase produced by Saccharomyces rouxii.

  • PDF

Effect of Ginseng Saponin on The Growth of Heat-Stressed Yeast Saccharomyces rouxii - Stimulation of Growth Recovery Rate of Heat-treated S. rouxii - (인삼 Saponin이 고온처리된 효모의 생육에 미치는 영향 - 열처리된 효모의 증식회복속도에 미치는 촉진효과 -)

  • 오영숙;김용수;이갑량
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.19 no.5
    • /
    • pp.375-380
    • /
    • 1990
  • It was attempted in this experiment to observe the effect of ginseng saponin on the growth and recovery rate of Saccharomyces rouxii which was shocked by heat-stress. The growth of S. rouxii was found most active when the saponin concentration in the malt extract broth was $10^{-3}%$ And the growth rate of S. rouxii grown on medium containing $10^{-3}%$ ginseng saponin was faster thanh that of the yeast grown on medium without saponin from the early log phase of the yeast. The growth recovery rate of S. rouxii which was heat-shockied by $80^{\circ}C$ - 30 minites was accelerated in the medium added ginseng saponin.

  • PDF

The Hypocholesterolemic Effect of Platycodi radix Saponin in Rats Fed a Hypercholesterol Diet (길경 saponin이 고콜레스테롤식이를 공급한 흰쥐의 지질대사에 미치는 영향)

  • Won, Hyang Rye
    • The Korean Journal of Community Living Science
    • /
    • v.24 no.1
    • /
    • pp.141-149
    • /
    • 2013
  • The objective of this study is to find the effect of improving hyperlipidemia through Platycodi radix saponin. A hypercholoesterol diet and Platycodi radix saponin methanol extract were supplemented to the 6 week old male Spargue Dawley rats for four weeks. Results showed that the serum total cholesterol, triglyceride and LDL-cholesterol level decreased in the group supplemented with Platycodi radix saponin methanol extract(p<0.05), however, the serum total lipid and HDL-cholesterol did not show any difference from the control group according to the supplement of Platycodi radix saponin methanol extract. In the group supplemented with Platycodi radix saponin methanol extract, the total cholesterol decreased by 20%, triglyceride by 36% and LDL-cholesterol by 25% respectively, and there was no difference according to the level of Platycodi radix saponin methanol extract. AI(atherogenic index) and CRF(cardiac risk factor) were low in the group supplemented with Platycodi radix saponin methanol extract. Total lipid, total cholesterol and triglyceride in liver and feces were significantly low in the group supplemented with the Platycodi radix saponin methanol extract compared with the control group(p<0.05). According to this result, it is expected that the Platycodi radix saponin methanol extract supplemented in the hypercholesterol diet can lower atherosclerosis, the cause of cardiovascular disease, by reducing not only serum cholesterol and triglyceride but also liver cholesterol and triglyceride.