• Title, Summary, Keyword: Saponin

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The Effect of Ginseng Saponin Fractions with Thyroid Hormone Secretion Regulatory Agents on cAMP Level in Cultured Rat Thyroid Glands (갑상선호르몬 분비조절물질과 인삼성분의 복합처리가 갑상선세포의 cAMP 양에 미치는 영향)

  • 정경훈;김세창·정노팔
    • Journal of Ginseng Research
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    • v.12 no.2
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    • pp.135-144
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    • 1988
  • When ginseng saponin fractions were treated with secretion regulatory agents, it increased cAMP level at the case of thyrotropin (but the amounts were small). Total saponin increased cAMP level at DEcAMP and isoproterenol, and decreased the level at carbachol and propranolol, but at NaF it had little effect. When diol saponin or triol saponin were treated with secretion regulatory agents, biol saponin decreased cAMP level but triol saponin increased it except for isoproterenol. Also, in propra%olol, which inhibits the increase of CAMP level, diol and triol saponin showed crossing effect, too. From the above results, ginseng saponin fractions are believed that it has the, effects of promotion or inhibition on cAMP production in the thyroid , both diol saponin and triol saponin have crossing effect on thyroid hormone secretion regulatory agents. The normalizatin action of ginseng saponin fractions is notable at the case of NaF and carbachol.

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Comparative Studies on the Effects of Total, Protopanaxadiol and ProtoBanaxatriol saponins of Ginseng 2. Their Effects on Blood Enzyme Activities in Rats (인삼 총사포닌, 디올계 및 트리올계 사포닌의 효과 2. 흰쥐의 몇가지 혈액효소활성에 미치는 영향)

  • 박창진;이동권
    • Journal of Ginseng Research
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    • v.5 no.1
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    • pp.49-55
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    • 1981
  • The effects of total, protopanaxadiol-and protopanaxatriol-saponins on the in vitro activities of several enzymes in rat serum were observed Alkaline phosphatase activity was increased 61 % by total saponin and 46% by protopanaxatriol-saponin, compared to control group. While SCOT activity was slightly decreased by total saponin and protopanaxatriol- saponin, it was slightly increased by Protopanaxadiol-saponin And while SCPT activity was slightly decreased by total saponin, it was increased by protopanaxadiol-saponin and protopanaxatriol-saponin. Creatine phosphokinase activity had a tendency to be increased by protopanaxatriol-saponin. Lactate dehydrogenase activities were increased in three saponin treated groups, but those were nonignificant. Compared to the control group, lipase activity was increased by all saponin samples. It was increased 157% by total saponin The increase in lipase activity by total saponin corresponded with the decrease in serum t total lipid by total saponin .

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생쥐 대식세포의 종양세포 치사활성에 미치는 인삼분획물과 지방다당류의 영향

  • Choe, Sang-Un;Jeong, No-Pal;Kim, Se-Chang
    • Journal of Ginseng Research
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    • v.14 no.3
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    • pp.364-372
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    • 1990
  • This experiment was performed to investigate the effects of ginseng saponin fractions (total saponin, triol saponin. diol saponin) and lipopolysacrharide (LPS) on the tllmoricidal activity of macrophage. The ginseng saponin fractions had little effect on the tumoricidal activity of macrophage (less than 10%). When the ginseng saponin fractions were treated with LPS, the effects of tumoricidal activation of macrophage increased a relatively high percent, and the total saponin and triol saponin (20-35%) were ulore effectual than diol saponin (15-25%). The effects of ginseng saponin and LPS on the tumoricidal activity of macrophage were mediated by the induction of macrophage-release factor(5) which has(have) the capacity of tumor cell killing. And the quantity of the (actors) was(were) increased by the contact of macrophage with tumor cell.

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Hypocholesterolemic Response to Karaya Saponin and Rhodobacter capsulatus in Broiler Chickens

  • Afrose, Sadia;Hossain, Md. Sharoare;Maki, Takaaki;Tsujii, Hirotada
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.6
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    • pp.733-741
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    • 2010
  • Dietary karaya saponin and Rhodobacter capsulatus (R. capsulatus) are known to have hypocholesterolemic actions, as reported in our previous studies. This study examined possible synergistic hypocholesterolemic effects of karaya saponin and R. capsulatus in broilers. A total of 150 broilers were allocated into 10 treatments: control, saponin 25 mg, saponin 50 mg, saponin 75 mg, saponin 25 mg+R. capsulatus 0.2 g, saponin 25 mg+R. capsulatus 0.4 g, saponin 50 mg+R. capsulatus 0.2 g, saponin 50 mg+R. capsulatus 0.4 g, saponin 75 mg+R. capsulatus 0.2 g and saponin 75 mg+R. capsulatus 0.4 g. Feed intake and feed efficiency were improved when karaya saponin and R. capsulatus were synergistically supplemented in the diet. Combinations of karaya saponin, especially supplementation of karaya saponin 50 mg+R. capsulatus 0.4 g were shown to have potential hypolipidemic actions in breast and thigh muscle cholesterol and triglycerides, serum cholesterol, low density lipoprotein-cholesterol and triglycerides, as well as improved high density lipoprotein (HDL)-cholesterol (p<0.05). Compared to the control, almost all the treatments significantly increased serum, liver and fecal concentrations of bile acids (p<0.05). Supplementation of both karaya saponin (75 mg) and saponin 50 mg+R. capsulatus 0.4 g reduced palmitic acid (C16:0) and stearic acid (C18:0) in a similar fashion (p<0.05). The ratios of PUFA:SFA or PUFA+MUFA:SFA in the thigh and breast muscle of broilers were greater in karaya saponin and R. capsulatus supplemented groups than in the control group. Thus, our study concluded that supplementation of karaya saponin synergistically with R. capsulatus in the diet of broilers is an effective way to obtain low-cholesterol, low-triglyceride and high HDL-cholesterol enriched poultry meat with a unique fatty acid balance.

Comparative Studies on the Effects of Total, Protopanaxadiol and Protopanaxatriol saponins of Ginseng 1. Their Effects on Lipid and Glucose Content in Rat Serum (인삼 총사포닌, 디올계 및 트리올계 사포닌의 효과 1. 흰쥐 혈청 지질 및 당함량에 미치는 영향)

  • 임창진;박은희;홍순근;이동권
    • Journal of Ginseng Research
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    • v.5 no.1
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    • pp.41-48
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    • 1981
  • Total saponin, protopanaxadiol-saponin and protopanaxatriol-saponin were isolated and purified from the side roots of red ginseng. After we administered them orally into rats during 5 weeks, we observed their effects on lipid and glucose content in rat serum. The change in body weight of protopanaxatriol- saponin treated group was slightly larger than those of other groups. Total lipid content in total saponin treated group showed an increase of about 20 % over that in control group. However, protopanaxadiol-saponin and protopanaxatriol- saponin treated groups showed no change. While triglyceride content in total saponin treated group decreased 29oyo compared to it s content in control group, its content in protopanaxatriol-saponin treated group increased 45%. Three saponin treated groups showed lower value than control group in total ant free cholesterol levels. While glucose content in total saponin treated group decreased slightly, that in Protopanaxadiol-saponin treated group decreased slightly compared to that in control group. And protopanaxatriol- saponin trented group showed the significant decrease of 25%. From these results, it is supposed that total saponin accelerates the conversion of lipid into glucose and that protopanaxatriol- saponin accelerates the conversion of glucose into lipid.

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An Isolation of Crude Saponin from Red-Ginseng Efflux by Diaion HP-20 Resin Adsorption Method (홍삼유출액으로부터 Diaion HP-20 수지 흡착법에 의한 조사포닌의 분리)

  • 곽이성;경종수;김시관;위재준
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.1
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    • pp.1-5
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    • 2001
  • This study was carried out to isolate saponin compounds from red-ginseng efflux, which was produced during the industrial processing of red-ginseng from fresh ginseng. We isolated crude saponin from the efflux extract (moisture content 35.0%) by using Diaion HP-20 adsorption method. Non-saponin fraction, which was adsorbed on Diaion HP-20 resin, was removed by eluating with $H_{2}O$ and 25% spirit. Then crude saponin was eluated with 95% spirit, continuously. Saponin in the eluated fractions was confirmed by TLC analysis. Crude saponin isolated from red ginseng efflux extract contained 12.10% of saponin. whereas those of white ginseng and red-ginseng were 3.30 and 3.39%, respectively. Ginsenoside contents showed the highest contents kin crude saponin from red ginseng efflux extract. Expacilly, the ginsenoside-$Rb_{1}$ and Re showed the highest contents in red-ginseng efflux extract when compared with those of white ginseng and red ginseng crude saponins. And the other ginsenosides except ginsenoside-$Rb_{1}$ and -Re also showed the highest contents in red ginseng efflux extract. However, the ratio of PD saponin (Panaxadiol saponin: $Rb_{1}+Rb_{2}$+Rc+Rd) to PT saponin (panaxatriol: $Re+Rg_{1}$) showed almost the same level when compared with those of ginseng saponin fractions. Ratio of PD/PT from red ginseng efflux extract was 1.99. Ratios of PD/PT from white ginseng and red ginseng were 1.85 and 1.84, respectively. Saponin purity, which was calculated by ratio percent of total ginsenoside to curde saponin content, was 45.90%. In case of white ginseng and red ginseng, the purities were 35.50 and 36.00%, respectively. However, by PHLC analysis, we confirmed that crude saponin isolated from red ginsengs. It suggested that crude saponin isolated from red ginseng ellux also would be useful component as ginseng saponins.

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Effect of Ginseng Saponin on Bacterial α-Amylase Activity (인삼(人蔘) Saponin이 세균(細菌) α-Amylase 활성(活性)에 미치는 영향(影響))

  • Do, Jae Ho;Kim, Sang Dal;Joo, Hyun Kyu
    • Microbiology and Biotechnology Letters
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    • v.13 no.1
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    • pp.7-11
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    • 1985
  • In order to investigate the biological activity of ginseng saponins, the effects of ginseng saponins on the reaction catalyzed by bacterial a-amylase were studied and the results obtained were summerized as follows. Bacterial ${\alpha}$-amylase activity was increased by the addition of protopanaxadiol (diol), protopanaxatriol (triol) and total saponin. Preincubation of ${\alpha}$-amylase with diol saponin at $40^{\circ}C$ for 3 min increased ${\alpha}$-amylase activity to the degree of 120%. In the protective effect on the heat denaturation of the enzyme, triol saponin protected the heat denaturation for 5 min at $60^{\circ}C$, but diol saponin accelerated the heat denaturation. The hydrolyzates of diol and triol saponin increased the enzyme activity more than the intact diol and triol saponin. In the catalysis system of bacterial ${\alpha}$-amylase, the addition of diol and triol saponin reduced the substrate inhibition in the presence of high concentration of the substrate.

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The Effects of Ginseng Components on the Signal Transduction in the Activation of Murine Macrophages (생쥐 대식세포의 활성시 신호전달에 미치는 인삼성분들의 영향)

  • 신은경;박한우
    • Journal of Ginseng Research
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    • v.20 no.2
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    • pp.159-167
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    • 1996
  • To study the effects of ginseng saponin components on the signal transduction in the ac tivation of murine macrophages, phagocytosis and Intracellular calcium concentration of peritoneal exuded mouse macrophages were examined. The phagocytosis was increased significantly after treatment with total saponin, diol-saponin, $Rg_1$ and $Rg_2$, but triol-saponin was unable to increase phagocytosis. The phagocytosis were increased when H7, a PKC inhibitor, was pretreated and increased significantly by saponin fractions except total saponin. Pertussis toxin, which inactivates G-protein, decreased the phagocytosis. But the phagocytosis was restored to the control level by saponin fractions and the phagocytosis was increased significantly by $Rg_2$ and $Rg_2$. The triol saponin increased phagocytosis approximately by 2-fold as compared with the TMB-8 treated group. Peritoneal exuded macrophages displayed a prominent rise in cytosolic calcium following treatment with triol-saponin, $Rg_1$, $Rg_2$ and $Rg_2$. Incubation of macrophages with PT resulted in an inhibition of cytosolic calcium mobilization, but increased cytosolic calcium mobilization with saponin fraction.

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Studies on the Anti-inflammatory Activity of Ginseng Total Saponin, Protopanaxadiol and Protopanaxatriol (인삼 Total Saponin, Protopanaxadiol 및 Protopanaxatriol의 소염작용에 관한 연구)

  • 조태순;이선미;정국현;이범구;이석용;박종대
    • Biomolecules & Therapeutics
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    • v.7 no.2
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    • pp.145-152
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    • 1999
  • In an attempt to elucidate the anti-inflammatory action of ginseng total saponin, protopanaxadiol and protopanaxatriol, the anti-inflammatory activity of three compounds was investigated under various acute and chronic inflammatory models. The blood vessel permeability was slightly inhibited by total saponin and protopanaxatriol treatments. Granuloma formation induced by 2% carrageenan was inhibited by total saponin and protopanaxatriol. The cotton-pellet granuloma formation was significantly inhibited by intraperitoneal injection of total saponin. Total saponin and protopanaxadiol inhibited leukocyte emigration and protein exudation in CMC-induced pouch but protopanaxauiol increased leukocyte emigration. The swelling of rat hind paw induced by 1% carrageenan was significantly inhibited by total saponin, protopanaxadiol and protopanaxatriol both single and 2 weeks treatments. Total saponin, protopanaxadiol and protopanaxatriol decreased the anti-inflammatory activity in adrenalectomized rat. Our results suggest that total saponin, protopanaxadiol and protopanaxatriol have potent anti-inflammatory activity, this may be mediated in part through stimulation of adrenal glands.

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Studies on the Saponins in the Shoot of Aralia Elata (I) -Separation and Content of the Saponins- (두릅나무 순의 Saponin에 관한 연구 (I) - Saponin 의 분리 및 함량 -)

  • Lee, Mahn-Jung;Kim, Young-Hee
    • Journal of the Korean Society of Food Culture
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    • v.5 no.2
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    • pp.235-241
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    • 1990
  • We extracted and analysed the saponin fraction in the shoot of Aralia Elata. The results were as follows. 1. Natural samples had higher contents and more kinds of saponin than those grown in the green-house. The contents and the kinds of saponin decreased by steam treatment in both samples while the contents increased with the growth. The number of saponins in the natural raw sample (Nr2) which was at the most suitable stage for use was 37. 2. The saponin pattern of Nr2, with the large Fh saponin was specifically different from those with different harvest time which had large ‘d’ fraction. But all the natural samples which were steam-treated showed similar saponin patterns irrespective of harvest time. The saponin patterns of the green-house grown samples were simpler and showed less change during growing compared with those of the natural ones. All the steamed green-house grown samples showed the similar saponin patterns.

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