• Title, Summary, Keyword: Salicylate

Search Result 153, Processing Time 0.038 seconds

Determination of Salicylate by Selective Poly(vinylchloride) Membrane Electrode Based on N,N'-1,4-Butylene Bis(3-methyl salicylidene iminato) Copper(II)

  • Mazloum Ardakani, M.;Jamshidpoor, M.;Naeimi, H.;Heidarnezhad, A.
    • Bulletin of the Korean Chemical Society
    • /
    • v.27 no.8
    • /
    • pp.1127-1132
    • /
    • 2006
  • A new salicylate-selective electrode based on the complex N,N'-1,4-butylene bis(3-methyl salicylidene iminato) copper(II) as the membrane carrier was developed. The electrode exhibits a good Nernstian slope of 59.1 ${\pm}$ 1.0 mV/decade and a linear range of $1.0\;{\times}\;10^{-6}$-1.0 M for salicylate. The limit of detection was $5.0\;{\times}\;10^{-7}$ M. It has a fast response time 10 s and can be used for more than three months. The selective coefficients were determined by the fixed interference method (FIM) and could be used in the pH range 4.5-10.5. It was employed as an indicator electrode for direct determination of salicylate in pharmaceutical samples.

Characterization of a salicylate-degrading strain of pseudomonas putida (Salicylate를 생분해하는 pseudomonas putida의 특성)

  • 홍순우;하영칠;이계준;배경숙
    • Korean Journal of Microbiology
    • /
    • v.24 no.1
    • /
    • pp.51-56
    • /
    • 1986
  • Eight strains of the bacteria capable of growing on salicylate as the sole carbon source were isolated form soil and river water. Three of these isolates were identified as Acinetobacter calcoaceticus (AcBl), Pseuomonas putida biotype B (PpB2), and P. putida biotype A (PpB3). Effects of temperature, pH and C source concentration on biodegradation of salicylate by PpB3 were wxamined. The optimum conditions were as follows; $30^{\circ}C$ for temperature, 7.0 for pH, and 10mM for C source concentration. Ultraviolet scanning spectrum of the salicylate was measured. The spectrum has two peaks at 225nm and 292nm. The spectra of the culture filtrates indicate that ring degradation of salicylate is accomplished.

  • PDF

Enhanced Anti-cancer Efficacy in MCF-7 Breast Cancer Cells by Combined Drugs of Metformin and Sodium Salicylate

  • Kim, Yun-Ji;Park, Hee-Bin;Kim, Pyung-Hwan;Park, James S.;Kim, Keun-Sik
    • Biomedical Science Letters
    • /
    • v.23 no.3
    • /
    • pp.290-294
    • /
    • 2017
  • Metformin or sodium salicylate is known to induce apoptosis and G0/G1 phase arrest in a variety of cancer cells. However, the anti-cancer effects of the combined treatments for these drugs-induced apoptosis are yet unclear. Here, we found that the combined treatment of metformin and sodium salicylate increased the efficacy of chemotherapeutics against breast cancer cells. These combined drugs significantly inhibited cellular proliferation and induced apoptosis at an earlier stage in human MCF-7 breast cancer cells. Also, co-treatments of metformin and sodium salicylate induced G1 cell cycle arrest in MCF-7 cells more effectively than either agent alone. Taken together, these results demonstrate that dual metformin/sodium salicylate treatment prevents proliferation of MCF-7 cells by inducing apoptosis and G1 cell cycle arrest.

A Determination of the Salicylate Content of Ingredients Commonly Used in Korean foods Suggested for Allergic Patients

  • Myung, Choonok;Lee, Kiwan;Nam, Haewon;Hong, Chein-Soo;Lee-Kim, Yang-Cha
    • Journal of Nutrition and Health
    • /
    • v.31 no.5
    • /
    • pp.949-956
    • /
    • 1998
  • Salicylate from plant foods and histamine from animal foods have been suggested to cause various food allergic symptoms. Salicylate regarded as one of the inducing agents f3r chronic urticaria and asthma in allergic patients was studied. There is much interest about the effects of salicylate in food allergy. It it recently suggested that the intake of salicylate from foods may have contributed to chronic urticaria and asthma. The purpose of this study is to analyze the salicylate content in various foods. 153 ingredients commonly used in Korean foods were analyzed by HPLC. Among the vegetables, chard(8.22$\mu\textrm{g}$/g), soybean sprout(5.13$\mu\textrm{g}$/g), perilla lean3.03$\mu\textrm{g}$/g), Chinese cabbage(2.25$\mu\textrm{g}$/g), carrot(2.16$\mu\textrm{g}$/g), and squash(1.33$\mu\textrm{g}$/g) con-rained higher amounts of salicylate as compared to other vegetables. Among fruits, oranges(5.13$\mu\textrm{g}$/g), grapefruits(2.90$\mu\textrm{g}$/g), and plums(1.65$\mu\textrm{g}$/g) contained the most. In animal foods, almost no salicylate was found except in chocolate milk(0.32$\mu\textrm{g}$/g). It was also found in black pepper(8.2$\mu\textrm{g}$/g), coffee(can)(5.00$\mu\textrm{g}$/g), mustard(4.16$\mu\textrm{g}$/g), curry powder(3.24$\mu\textrm{g}$/g), kochujang(2.37$\mu\textrm{g}$/g), cream powder(1.19$\mu\textrm{g}$/g), brown rice(1.04$\mu\textrm{g}$/g) and sesame oil(1.00$\mu\textrm{g}$/g). All information found in this study can be used as nutritional education materials in potentially allergic people for a prudent diet plan and can also provide proper dietary guidelines for these allergic patients. (Korean J Nutrition 31(5) 949-956, 1998)

  • PDF

Characterization of biphenyl biodegradation, and regulation of iphenyl catabolism in alcaligenes xylosoxydans

  • Lee, Na-Ri;On, Hwa-Young;Jeong, Min-Seong;Kim, Chi-Kyung;Park, Yong-Keun;Ka, Jong-Ok;Min, Kyung-Hee
    • Journal of Microbiology
    • /
    • v.35 no.2
    • /
    • pp.141-148
    • /
    • 1997
  • Alcaligenes xylosoxydans strain SMN3 capable of utilizing biphenyl grew not only on phenol, and benzoate, but also on salicylate. Catabolisms of biphenyl and salicylate appear to be interrelated since benzoate is a common metabolic intermediate of these compounds. Enzyme levels in the excatechol 2. 3-dioxygenas which is meta-cleavage enzyme of catechol, but did not induce catechol 1, 2-dioxygenase. All the oxidative enzymes of biphenyl and 2, 3,-dihydroxybiphenyl (23DHBP) were induced when the cells were grown on biphenyl and salicylate, respectively. Biphenyl and salicylate could be a good inducer in the oxidation of biphenyl and 2, 3-dihydroxybiphenyl. The two enzymes for the degradation of biphenyl and salicylate were induced after growth on either biphenyl or salicylate, suggesting the presence of a common regulatory element. However, benzoate could not induce the enzymes responsible for the oxidation of these compounds. Biphenyl and salicylate were good inducers for indigo formation due to the activity of biphenyl dioxygenase. These results suggested that indole oxidation is a property of bacterial dioxygenase that form cis-dihydrodiols from aromatic hydrocarbon including biphenyl.

  • PDF

Influence of Matrixes on Nylon Microcapsules of Sodium Salicylate (Sodium salicylate 나일론 마이크로캅셀 제조에 미치는 Matrix의 영향)

  • Ku, Young-Soon;Yoo, Jung-Hoo
    • YAKHAK HOEJI
    • /
    • v.28 no.4
    • /
    • pp.217-221
    • /
    • 1984
  • Nylon microcapsules of sodium salicylate containing three different matrixes, acacia, gelatin and formalized gelatin, were prepared by interfacial polymerization and the effect of the matrix on the dissolution rate of sodium salicylate from its nylon microcapsule was investigated. The microcapsules were spherical and their particle diameter increased in proportion to the amount of matrix. The surface was different from each other according to the kind and the amount of matrix when observed by the scanning electron microscopy. The dissolution rate of sodium salicylate from its microcapsules was decreased by increase of the amount of matrix and the formalized gelatin most decreased the dissolution rate of drugs.

  • PDF

Isolation of Salicylate-Degrading Plasmid from Pseudomonas putida (Pseudomonas putida로 부터 salicylate 분해 plasmid의 분리)

  • 배경숙;나종욱;강사욱;홍순우;하영칠;이계준
    • Korean Journal of Microbiology
    • /
    • v.24 no.2
    • /
    • pp.106-112
    • /
    • 1986
  • The large plasmid (about 180 megadaltons) was isolated from the aquatic strain of Pseudomonas which was found to degrade salicylate. It was found that the plasmid could be isolated under gentle conditions in comparison with other methods. The yield of covalently closed circular DNA was enganced by heat treatment at $55^{\circ}C$ after denaturing the chromosomal DNA with alkaline sodium dodecyl sulfate (pH 12.45), and the plasmid DNA was selectively concentrated by utilizing 10% polyethylene glycol as final concentration. It was also found that the cured strains with mitomycin C did not show any growth on the medium containing salicylat6e, therefore, it was concluded that the plasmid might play and important role on the salicylate degradation.

  • PDF

Sodium Salicylate Inhibits Expression of COX-2 Through Suppression of ERK and Subsequent $NF-{\kappa}B$ Activation in Rat Ventricular Cardiomyocytes

  • Kwon, Keun-Sang;Chae, Han-Jung
    • Archives of Pharmacal Research
    • /
    • v.26 no.7
    • /
    • pp.545-553
    • /
    • 2003
  • The expression of cyclooxygenase-2 (COX-2) is a characteristic response to inflammation, which can be inhibited with sodium salicylate. IL-1$\beta$ and TNF-$\alpha$ can induce extracellular signal-regulated kinase (ERK), IKK, IkB degradation and NF-$\kappa$B activation. Salicylate inhibited the IL-1$\beta$ and TNF-$\alpha$-induced COX-2 expressions, regulated the activation of ERK, IKK and IkB degradation, and the subsequent activation of NF-$\kappa$B, in neonatal rat ventricular cardiomyocytes. The inhibition of the ERK pathway, with a selective inhibitor, PD098059, blocked the expressions of IL-1$\beta$ and TNF-$\alpha$-induced COX-2 and $PGE_2$ release. The antioxidant, N-acetyl-cysteine, also reduced the glutathione or catalase- attenuated COX-2 expressions in IL-1$\beta$ and TNF-$\alpha$-treated cells. This antioxidant also inhibited the activation of ERK and NF-$\kappa$B in neonatal rat cardiomyocytes. In addition, IL-1$\beta$ and TNF-$\alpha$-stimulated the release of reactive oxygen species (ROS) in the cardiomyocytes. However, salicylate had no inhibitory effect on the release of ROS in the DCFDA assay. The results showed that salicylate inhibited the activation of ERK and IKK, I$\kappa$B degradation and NF-$\kappa$B activation, independently of the release of ROS, which suggested that salicylate exerts its anti-inflammatory action through the inhibition of ERK, IKK, IkB and NF-$\kappa$B, and the resultant COX-2 expression pathway in neonatal rat ventricular cardiomyocytes.

Salicylate Regulates Cyclooxygenase-2 Expression through ERK and Subsequent $NF-_kB$ Activation in Osteoblasts

  • Chae, Han-Jung;Lee, Jun-Ki;Byun, Joung-Ouk;Chae, Soo-Wan;Kim, Hyung-Ryong
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.7 no.4
    • /
    • pp.239-246
    • /
    • 2003
  • The expression of cyclooxygenase-2 (COX-2) is a characteristic response to inflammation and can be inhibited with sodium salicylate. $TNF-{\alpha}$ plus $IFN-{\gamma}$ can induce extracellular signal-regulated kinase (ERK), IKK, $I{\kappa}B$ degradation and NF-${\kappa}B$ activation. The inhibition of the ERK pathway with selective inhibitor, PD098059, blocked cytokine-induced COX-2 expression and $PGE_2$ release. Salicylate treatment inhibited COX-2 expression induced by $TNF-{\alpha}$/$IFN-{\gamma}$ and regulated the activation of ERK, IKK and $I{\kappa}B$ degradation and subsequent NF-${\kappa}B$ activation in MC3T3E1 osteoblasts. Furthermore, antioxidants such as catalase, N-acetyl-cysteine or reduced glutathione attenuated COX-2 expression in combined cytokines-treated cells, and also inhibited the activation of ERK, IKK and NF-${\kappa}B$ in MC3T3E1 osteoblasts. In addition, $TNF-{\alpha}$/$IFN-{\gamma}$ stimulated ROS release in the osteoblasts. However, salicylate had no obvious effect on ROS release in DCFDA assay. The results showed that salicylate inhibited the activation of ERK and IKK, $I{\kappa}B$ degradation and NF-${\kappa}B$ activation independent of ROS release and suggested that salicylate exerts its anti-inflammatory action in part through inhibition of ERK, IKK, $I{\kappa}B$, $NF-{\kappa}B$ and resultant COX-2 expression pathway.