• Title, Summary, Keyword: SNARE

Search Result 45, Processing Time 0.066 seconds

Prion Protein Does Not Interfere with SNARE Complex Formation and Membrane Fusion

  • Yang, Yoo-Soo;Shin, Jae-Il;Shin, Jae-Yoon;Oh, Jung-Mi;Lee, Sang-Ho;Yang, Joo-Sung;Kweon, Dae-Hyuk
    • Food Science and Biotechnology
    • /
    • v.18 no.3
    • /
    • pp.782-787
    • /
    • 2009
  • In prion disease, spongiform neurodegeneration is preceded by earlier synaptic dysfunction. There is evidence that soluble N-ethylmaleimide sensitive factor attachment receptor (SNARE) complex formation is reduced in scrapie-infected in vivo models, which might explain this synaptic dysfunction because SNARE complex plays a crucial role in neuroexocytosis. In the present study, however, it is shown that prion protein (PrP) does not interfere with SNARE complex formation of 3 SNARE proteins: syntaxin 1a, SNAP-25, and synaptobrevin. Sodium dodecyl sulfate-resistant complex formation, SNAREdriven membrane fusion, and neuroexocytosis of PC12 cells were not altered by PrP. Thus, PrP does not alter synaptic function by directly interfering with SNARE complex formation.

Endovascular Rescue Method for Undesirably Stretched Coil

  • Cho, Jae Hoon
    • Journal of Korean Neurosurgical Society
    • /
    • v.56 no.4
    • /
    • pp.364-367
    • /
    • 2014
  • Undesirable detachment or stretching of coils within the parent artery during aneurysm embolization can be related with thrombus formation, which can be caused occlusion of parent artery or embolic event(s). To escape from this situation, several rescue methods have been reported. A case with undesirably stretched coil in which another rescue method was used, is presented. When the stretched coil is still located in the coil delivery microcatheter, the stretched coil can be removed safely using a snare and a handmade monorail microcatheter. After a snare is lodged in the handmade monorail microcatheter, the snare is introduced over the coil delivery micorcatheter and located in the distal part of the stretched coil. After then, the handmade monorail microcatheter captures the stretched coil and the snare as one unit. This technique using a handmade monorail microcatheter and a snare can be a good rescue modality for the undesirably stretched coil, still remained within the coil delivery microcatheter.

Heartworm Extraction in a Pitbull Terrier Dog with Heavy Worm Burden Using A Loop Snare (심한 심장사상충 감염증에 걸린 핏불테리어 개에서 Loop Snare를 이용한 심장사상충 제거술)

  • Kim, Ji-Yoon;Yoon, Won-Kyoung;Yang, Hye-Mi;Choi, Won-Jin;Kim, Hyun-Wook;Hyun, Changbaig
    • Journal of Veterinary Clinics
    • /
    • v.31 no.5
    • /
    • pp.412-416
    • /
    • 2014
  • A 7-year-old intact male Pitbull terrier dog was presented with complaints of marked abdominal distension and severe exercise intolerance. Diagnostic studies found a right-sided cardiomegaly with marked dilation of pulmonary arteries, diffuse interstitial pulmonary infiltration and heavy worm burden in the right atrium and ventricle. Color and spectral Doppler echocardiography also revealed tricuspid and pulmonic regurgitation indicating severe pulmonary hypertension. The 104 heartworms were removed by interventional extraction using a loop snare (Snare, Boston Scientific, USA) through external jugular vein. After heartworm removals, the clinical condition of this dog was remarkably improved. To author's best knowledge, this is the first case of interventional heartworm extraction using a loop snare in Korea.

SNARE Assembly and Membrane Fusion: A Paramagnetic Electron Magnetic Resonance Study

  • Kweon, Dae-Hyuk
    • Proceedings of the Korean Biophysical Society Conference
    • /
    • /
    • pp.32-32
    • /
    • 2003
  • In the neuron, SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) assembly plays a central role in driving membrane fusion, a required process for neurotransmitter release. In the cytoplasm, vesicular SNARE VAMP2 (vesicle-associated membrane protein 2) engages with two plasma membrane SNAREs syntaxin 1A and SNAP-25 (synaptosome-associated protein of 25 kDa) to form the core complex that bridges two membranes. While various factors regulate SNARE assembly, the membrane also plays the regulatory role by trapping VAMP2 in the membrane. The fluorescence and EPR analyses revealed that the insertion of seven C-terminal core-forming residues into the membrane controls complex formation of the entire core region, even though preceding 54 core-forming residues are fully exposed and freely moving. When two interfacial Trp residues in this region were replaced with hydrophilic serine residues, the mutation supported rapid complex formation.

  • PDF

Functions of the Plant Qbc SNARE SNAP25 in Cytokinesis and Biotic and Abiotic Stress Responses

  • Won, Kang-Hee;Kim, Hyeran
    • Molecules and Cells
    • /
    • v.43 no.4
    • /
    • pp.313-322
    • /
    • 2020
  • Eukaryotes transport biomolecules between intracellular organelles and between cells and the environment via vesicle trafficking. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE proteins) play pivotal roles in vesicle and membrane trafficking. These proteins are categorized as Qa, Qb, Qc, and R SNAREs and form a complex that induces vesicle fusion for targeting of vesicle cargos. As the core components of the SNARE complex, the SNAP25 Qbc SNAREs perform various functions related to cellular homeostasis. The Arabidopsis thaliana SNAP25 homolog AtSNAP33 interacts with Qa and R SNAREs and plays a key role in cytokinesis and in triggering innate immune responses. However, other Arabidopsis SNAP25 homologs, such as AtSNAP29 and AtSNAP30, are not well studied; this includes their localization, interactions, structures, and functions. Here, we discuss three biological functions of plant SNAP25 orthologs in the context of AtSNAP33 and highlight recent findings on SNAP25 orthologs in various plants. We propose future directions for determining the roles of the less well-characterized AtSNAP29 and AtSNAP30 proteins.

A Study on the Evaluation of Oil-adsorption Characteristics and Policy Guideline of Oil Snare (오일스네어에 대한 오일 흡착기준 정립 및 고시방향 연구)

  • Jin, Y.M.;You, J.Y.;Choi, S.S.;Joo, A.R.;Lee, J.H.;Lee, Soon-Hong
    • Journal of the Korean Society of Safety
    • /
    • v.34 no.6
    • /
    • pp.22-28
    • /
    • 2019
  • In South Korea, the enact of Korean Coast Guard Act-1 manages physical and chemical oil-dispersants. Oil snare, which is made of polypropylene, is newly added to the aforementioned act, and it has advantage on the ease of recovery compare to other adsorbents. This study synthesized bunker B-oil with diesel-oil and bunker C-oil to perform an adsorption test based on three samples which were manufactured in South Korea. As a result, adsorption test revealed 5.2 g/g more adsorption than the previous results from the act. Additional toluene test revealed that all the samples satisfied 90.0%, however coloured samples could release its pigment on the marine environment. Thus, colorless samples are recommended on the risk management of marine accidents. The study on the basic direction of the calculation of the test items and the standard value for the quality control of the oil snare was also carried out.

Interaction of Human α-Synuclein with VTI1B May Modulate Vesicle Trafficking

  • Lee, Hak-Joo;Lee, Kyung-Hee;Im, Ha-Na
    • Bulletin of the Korean Chemical Society
    • /
    • v.33 no.9
    • /
    • pp.3071-3075
    • /
    • 2012
  • Human ${\alpha}$-synuclein is the major component of the protein aggregates known as Lewy bodies or Lewy neurites, which define the intracellular lesions of Parkinson's disease. Despite extensive efforts, the physiological function of ${\alpha}$-synuclein has not yet been elucidated in detail. As an approach to defining its function, proteins that interacted with ${\alpha}$-synuclein were screened in phage display assays. The SNARE protein vesicle t-SNARE-interacting protein homologous 1B (VTI1B) was identified as an interacting partner. A selective interaction between ${\alpha}$-synuclein and VTI1B was confirmed by coimmunoprecipitation and GST pull-down assays. VTI1B and ${\alpha}$-synuclein were colocalized in N2a neuronal cells, and overexpression of ${\alpha}$-synuclein changed the subcellular localization of VTI1B to be more dispersed throughout the cytosol. Considering the role played by VTI1B, ${\alpha}$-synuclein is likely to modulate vesicle trafficking by interacting with a SNARE complex.

Successful Removal of Endobronchial Lipoma by Flexible Bronchoscopy Using Electrosurgical Snare

  • Yun, Seong Cheol;Na, Moon Jun;Choi, Eugene;Kwon, Sun Jung;Lee, Seong Ju;Oh, Sun Hee;Cha, Eun Jung;Son, Ji Woong
    • Tuberculosis and Respiratory Diseases
    • /
    • v.74 no.2
    • /
    • pp.82-85
    • /
    • 2013
  • A 62-year-old man with a chronic cough presented with atelectasis of the left upper lobe on chest X-ray. Chest computed tomography showed an atelectasis in the left upper lobe with bronchial wall thickening, stenosis, dilatation, and mucoid impaction. We performed bronchoscopy and found a well-circumscribed mass on the left upper lobe bronchus. The mass was removed by flexible bronchoscopy using an electrosurgical snare and diagnosed with lipoma. An endobronchial lipoma is a rare benign tumor that can be treated by a surgical or endoscopic approach. We report the successful removal of endobronchial lipoma via flexible bronchoscopic electrosurgical snare.