• Title, Summary, Keyword: Relative Activity

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Studies on the cell cycle of saccharomyces cerevisiae by electron spin resonance spectroscopy (전자스핀공명스펙트럼에 의한 saccharomyces cerevisiae의 세포환 연구)

  • 임형순;강사욱
    • Korean Journal of Microbiology
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    • v.26 no.1
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    • pp.44-51
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    • 1988
  • The intracellular free radicals produced at different stages of cell cycle of Saccharomyces cerevisiae ATCC 24858 were investigated by means of electron spin resonance(ESR) spectroscopy. The synchronized cells by repeated starvation and refeeding revealed different ESR spectral pattern compared to that of asynchronized cells. Each spectrum centered at g=2.005, which indicates free radicals. The relative spin concentration was maximat at the end of DNA increase. The variation of the relative spin concentration at each distinct stage of the cell cycle was evaluated in relation to ascorbate concentration, L-galactonolactone oxidase activity, and ascorbate oxidase activity. The highest activities of L-galactonolactone oxidase and ascorbate oxidase were detected just before and at the maximum of relative spin concentration, respectively. And ascorbate concentration fluctuated through each stage of cell cycle with the changes of relative spin concentration, L-galactonolactone oxidase activity, and ascorbate oxidase activity. Thus it is suggested that intracellular free radicals should be related to cell cycle, interacted with ascorbate, and may play an important role in the cell cycle of Saccharomyces cerevisiae.

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Alcohol Metabolizing Activity of Fermented Sea Tangle Juice (Lactobacillilus brevis를 이용한 다시마 발효물의 알코올 분해 활성)

  • Kang, Young-Mi;Lee, Bae-Jin;Kim, Jin-Su
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.1
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    • pp.1-5
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    • 2010
  • Alcohol metabolizing activity of fermented sea tangle juice (FSYJ) using Lactobacillilus brevis BJ20 were evaluated by measuring relative alcohol dehydrogenease (ADH) and aldehyde dehydrogenase (ALDH) activities. According to the results of MTI assay. the fermented sea tangle juice by Lactobacillilus brevis BJ20 appeared safe in the cytotocxity. The relative ADH activity of FSTJ showed 124% at 10 mg/mL, which increased with increasing concentration. The relative ALDH activity showed, however, insignificant difference (P>0.05) between concentrations of FSTJ up to 50 mg/mL. These results suggested that fermented sea tangle juice by L. brevis BJ20 could be used as a potential material for metabolizing alcohol.

Study on Estrogenic Activities of Pesticide Chemicals using E-screen Assay (E-Screen assay법을 이용한 농약화학물질의 에스트로겐 활성 연구)

  • Han, Sang Guk
    • Journal of Environmental Science International
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    • v.13 no.6
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    • pp.591-597
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    • 2004
  • In this study, sixty chemicals including 47 pesticides were screened for estrogenic activity using E-screen assay. MCF-7 cell, used in E-screen assay, is known to be proliferated by addition of estrogenic chemicals. Eight of the measured pesticides showed estrogenic activity at the concentration range of 100-10,000nM. Their relative proliferative effect (RPE) and the relative proliferative potency (RPP) were 20-65% and 0.01-1.0%, respectively, when compared with 1.0nM of $\beta-Estradiol-17-acetate(E_{2}).$ DDVP and diazinone showed most strong estrogenic potency(RPP; 1.0%) and effect(RPE; 65%) of the eight pesticides. These results are in agreement with estrogenic activity of bisphenol A is known as a positive endocrine disrupter. Also, in this study, paraquat, DDVP, 4-chloro-3-methylphenol, 2,4,6-trichlorophenol and diazinon of the measured pesticides are estimated to estrogenic chemical.

Regulation of Lipoprotein Lipase by Fasting in Epididymal and Mesenteric Adipocytes of Rats

  • Lee, Jae Joon;Chung, Chung Soo;Lee, Myung Yul
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.5
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    • pp.715-722
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    • 2008
  • There are marked variations in the activity of lipoprotein lipase (LPL) among adipose depots. The aim of this study was to compare the mechanisms of 24 h of fasting on LPL regulation between epididymal (EPI) adipocytes and mesenteric (MES) adipocytes in rats. 1-Day fasting consistently decreased activities of heparin-releasable LPL, total extractable LPL and cellular LPL markedly in both EPI and MES fat pads. LPL activity in MES fat pads was relatively lower than in the EPI fat pads. Consistent with data on LPL activity, the levels of expression of LPL mRNA in both nutritional states were lower in MES than EPI adipose tissue and isolated adipocytes. The decreased LPL activity after 1 day of fasting in MES adipocytes was explained mainly by a 50% decrease in the relative abundance of LPL mRNA level and a parallel 50% decrease in relative rate of LPL synthesis. In contrast, fasting of 1 day in EPI adipocytes decreased total LPL activity by 47% but did not affect LPL mRNA level or relative rate of LPL synthesis. A decrease in overall protein synthesis contributed to the decreased LPL activity after 1 day fasting both in EPI and MES adipocytes. In MES adipocytes the decrease in LPL activity, LPL mRNA and LPL synthesis were comparable, but in EPI adipocytes the changes in LPL activity were substantially larger than the changes in LPL mRNA level and LPL synthesis. Therefore, fasting decreased fat cell size, LPL activity, LPL mRNA level and relative rate of LPL synthesis in rats, and these effects were more marked in the MES adipocytes. These results clearly demonstrate the regional variations in the metabolic response of adipose tissue and LPL functions to fasting.

Sex-related Differences in Rat Hepatic Cytochromes P450 Expression Following Treatment with Phenobarbital or 3-Methylcholanthrene

  • Lee, Yoon-Sook;Park, Sang-Shin;Kim, Nak-Doo
    • Archives of Pharmacal Research
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    • v.15 no.1
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    • pp.78-86
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    • 1992
  • The induction of hepatic cytochromes P450 and metabolic effects have been examined in male and female Sprague-Dawley rats following treatment with either phenobarbital or 3-methylcholanthrene. Hepatic cytochrome P450 levels were higher in males than in females by ~40%. Treatment of male and female rats with phenobarbital or 3-methylcholanthrene resulted in an ~1.6 and 2-fold increase, respectively, in heptic microsomal cytochrome P450 levels in both sexes, relative to untreated animals. Immunoblot analyses were performed to compare sex-related changes in P450 levels. Hepatic P45IIB1 levels in males were greater than those in females following phenobarbital treatment. 3-Methylcholanthrene-induced male hepatic microsomes exhibited greater levels of P450 females failed to exhibit a band. Mab PCN 2-13-1 against P-45-IIIA recognized an intense in uninduced microsomes from female rats. The levels of P450IIIA in males were increased 2 to 3-fold following treatment with phenobarbital, while the increase of IIIA levels in females by phenobarbital was minimal, as monitored by immunoblot analysis. Solid phase radiommunoassay using monoclonal antibodies supported the results of immunoblot analysis. Phenobarbital treatment caused a 6.5-fold increase in the monoclonal iantibody binding to IIBI in males, whereas treatment of females with phenobarbital resulted of IA levels by 3-methylcholanthrene was also greater in females than in males (10-vs. 8-fold) although the levels of induced IA were comparable inboth sexes, as assessed by radiommunoassay. Radioimmunoassay also showed that hepatic IIEI level was 1.5-fold higher in males than in females and that either phenobarbital or 3-methylcholanthrene treatment caused 80% to 40% decrease in IIEL levels, relative to control, in both sexes. Sex-related metabolic activities were examined in hepatic microsomes. Hexobarbital hydroxylase activity was 2-to 3-fold higher in uninduced microsomes from males than that from females. This hydroxylase activity was increased 2-and 3-fold in males and females, respectively, following phenobarbital treatment, as compared to controls. Addition females produced 64% and 84% inhibition of hexobarbital oxidation, respectively. Aryl hydrocarbon hydroxylase activity was increased -12 and 26-fold in males and females respectively. Following phenobarbital treatment, as compared to controls. Addition of anti-P450IIB1 antibody to phenobarbital-induced hepatic microsomes from males and females produced 64% and 84% inhibition of hexobarbital oxidation, respectively. Aryl hydrocarbon hydroxylase activity was increased -12 and 26 fold in males and females, respectively, following 3-methylcholanthrene treatment relative to controls. The anti-P-450IA antibody inhibitable rate of aryl hydrocarbon hydroxylase activity was comparable in both sexes following 3-methylcholanthrene treatment relative to controls. The anti-P450LA antibody inhibitable rate of aryl hydrocarbon hydroxylase activity was comparable in both sexes following 3-methylcholanthrene treatment (-70%). These results demonstrate that levels of hepatic P450IIB1 or P450IA are greater in male than in female for untreated, phenobarbital-or-3-methylcholanthrene treated rats. In addition, the relative for untreated phenobarbital-or 3-methylcholanthrene treated rats. In addition, the relative increase of phenobarbital-or 3-methylcholanthrene treated rats. In addition, the relative increase of phenobarbital-or 3-methylcholanthrene treated rats. In addition, the relative increase of P450IIB1 or IA phenobarbital or 3-methylcholanthrene is more significant in females.

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Relationship Between Relative Water Content and Ascorbate Redox Enzymes Activity in Lettuce Leaves Subjected to Soil Water Stress (토양 수분 Stress에 따른 상추의 엽중 상대수분 함량과 아스코브산 관련 효소 활성도)

  • Kang, Sang-Jae;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.1
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    • pp.32-39
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    • 2013
  • The relationship between relative water contents of lettuce leaves and biochemical activities in lettuce was examined in this study to explore an adaptation response of lettuce to water stress from soils. Soil water contents and relative water contents of leaves were positively related to show $R^2$=0.8728. Hydrogen peroxide contents of leaves rapidly increased with reduction of soil water content, whereas soluble protein contents and dry matters rapidly decreased. And chlorophyll a and b contents of leaves decreased with increase in carotenoid content. Furthermore, the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) increased dramatically, and mRNA transcript levels of APX, MDHAR and DHAR also increased. Relationship of relative water content of lettuce leaves to hydrogen peroxide, to ascorbate peroxidase activity, to dehydroascorbate reductase activity, and to monodehydroascorbate reductase activity was shown to be positively correlated. It is highly plausible from this study that these enzyme activities could be developed as an indicator of water states in soils.

Comparison of Trunk Stabilization Maneuver on Surface Electromyographic Activity of Trunk Muscle (몸통 안정화 방법에 따른 몸통근육의 근활성도 비교)

  • Kim, Hyunhee;Chung, Sin-Ho
    • Journal of muscle and joint health
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    • v.20 no.3
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    • pp.189-196
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    • 2013
  • Purpose: The purpose of this study was to investigate electromyographic (EMG) activity of deep and superficial trunk muscles during trunk stabilization exercises with and without stabilization maneuvers. Methods: The relative muscle activity ratios and local muscle activities of 25 healthy males were measured using the 8 channel surface EMG system (Myosystem 1400A, Noraxon Inc., U.S.A). The surface EMG activities were tested during performing abdominal hollowing maneuver (AHM), abdominal bracing maneuver (ABM) and no stabilization maneuver (NSM) in random order. Data were analyzed using $1{\times}3$ repeated measures ANOVA. Results: During bridging exercises, the EMG activity ratio of transverse abdominis/internal oblique abdominis relative to rectus abdominis was significantly lower in NSM than in AHM and ABM. During bridging and kneeling exercises, the EMG activity ratio of multifidus relative to erector spinae was significantly higher in AHM than in NSM. Conclusion: The AHM can be clinically used by the physical therapist to activate selectively the trunk muscles when designing selective training programs for patients.

Effects of Astragali Radix extract on the Cell Mediated Immunotoxicity of Zinc Chloride (염화아연의 세포성 면역독성에 미치는 황기 추출물의 효과)

  • 채병숙;신태용
    • YAKHAK HOEJI
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    • v.43 no.1
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    • pp.98-103
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    • 1999
  • Effects of Astragali Radix extract (AG) on the cell mediated-and nonlpecific immunotoxic responses of zinc chloride (Zn) were studied usign ICR mice. Mice were divided into 4 groups (10 mice/group), and Zn was given to the mice 1 hr after i.p. injection with 0.5g/kg of AG by i.p. injection daily for 10 days at a dose of 25 mg/kg. Immune responses on the responses on the relative weight of thymus, delayed-type hypersensitivity to SRBC (DTH), phagocytic activity and circulating leukocytes were evaluated. Zn treatment decreased body weight gain, the relative weight of thymus, DTH and circulating leukocytes compared with those in controls. AG treatment increased DTH, phagocytic activity and circulating leukocytes compared with those in controls. Combination of AG and Zn increased DTH and circulating leukocytes compared with those in controls, but decreased body weight gain and the relative weight of thymus. These findings indicated that AG decreased immunotoxicity of Zn on the DTH and circulating leukocytes.

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Effects of Cranial Electrotherapy Stimulation on Electrocephalogram

  • Lee, Jeongwoo;Lee, Hyejein;Park, Woongsik
    • Journal of International Academy of Physical Therapy Research
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    • v.10 no.1
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    • pp.1687-1694
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    • 2019
  • Background: Although cranial electrotherapy stimulation (CES) is reported to have positive effects on mental functions such as depression and sleep improvement, detailed studies regarding awakening, attention and concentration among brain waves reflecting brain activity are lacking. Objective: To examine the effects of cranial electrotherapy stimulation (CES) on various electroencephalograms (EEGs) reflecting brain activities. Design: Randomized controlled clinical trial (single blind) Methods: This study selected 30 healthy adult women in their 20s who volunteered for this experiment. A total of 30 subjects were randomly assigned to three groups (Sham group, 0.5 Hz CES group, and 100 Hz CES group). EEGs were measured before and after the single CES, and the results were compared and analyzed. Results: The relative theta, alpha, and gamma waves indicated no significant differences in the interaction effects between time and group. The relative fast alpha wave only showed significant differences in the interaction effects between time and group in P4. The relative slow beta wave only indicated statistically significant differences in the interaction effects between time and group in T3 and T4. The relative mid and fast beta waves showed statistically significant differences in the interaction effects between time and group in all areas. Conclusions: These results suggest that a CES of 0.5 Hz awakens consciousness and has a positive influence on brain activity, while a CES of 100 Hz has a positive influence on thinking activity accompanying mental load during concentrating on one subject.

The Influence of Disease Activity and Uncertainty on Anxiety and Depression in Patients with Ankylosing Spondylitis (강직성 척추염 환자의 질병활성도와 불확실성이 불안과 우울에 미치는 영향)

  • Lim, Jong-Mi;Cho, Ok-Hee
    • Journal of Korean Academic Society of Home Health Care Nursing
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    • v.24 no.1
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    • pp.61-68
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    • 2017
  • Purpose: The purpose of this study was to understand how disease activity and uncertainty influence anxiety and depression in patients with ankylosing spondylitis Methods: Participants were 125 patients with ankylosing spondylitis who had attended the rheumatology division of a university hospital. A structured questionnaire was used to assess disease activity, uncertainty, anxiety, and depression. The data gathered were analyzed using t-tests, ANOVAs, Pearson correlation coefficients, and a multiple regression. Results: Differences were observed in anxiety based on job status, and in depression based on age, marriage, and job status. Uncertainty and disease activity predicted patients' anxiety and explained 40% of the variance in this measure, and the relative influence of uncertainty (${\beta}=.38$, p<.001) was larger than that of disease activity (${\beta}=.30$, p<.001). Furthermore, uncertainty and disease activity predicted depression and explained 36% of the variance therein, and the relative influence of uncertainty (${\beta}=.27$, p=.002) and disease activity (${\beta}=.27$, p=.003) was similar. Conclusion: This study confirmed that disease activity and uncertainty influenced anxiety and depression in patients with ankylosing spondylitis. Therefore, efforts to decrease anxiety and depression in patients with this condition must take into consideration disease activity when implementing nursing interventions and should include strategies to lower uncertainty.