• Title, Summary, Keyword: Radioprotective Effect

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Radioprotective Effect of Cyclo(L-Phenylalanyl-L-Prolyl) on Irradiated Rat Lung

  • Lee, Keyong-Ho;Rhee, Ki-Hyeong
    • Journal of Microbiology and Biotechnology
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    • v.18 no.2
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    • pp.369-376
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    • 2008
  • In the present study, we investigated the radioprotective effect of cyclo(L-phenylalanyl-L-prolyl) on irradiated rat lungs to determine its potential as a radioprotective agent. We found that early lung damage induced by irradiation was reduced by treatment with 40mg/kg of cyclo(L-phenylalanyl-L-prolyl) in the latent and early pneumonitis phases. Expression of $TNF-{\alpha}\;and\;TGF-{\beta}1$ at 2 and TGF-${\beta}1$ at 8 weeks post-irradiation was decreased in animals that received both radiation and cyclo(L-phenylalanyl-L-prolyl) compared with animals that received radiation alone. Evidence indicated that the proinflammatory cytokine TNF-${\alpha}$ and the fibrogenic cytokine TGF-${\beta}1$ likely play a role in the radioprotective effect of cyclo(L-phenylalanyl-L-prolyl). However, besides TNF-${\alpha}$ and TGF-${\beta}1$ expressions, the precise mechanism by which cyclo(L-phenylalanyl-L-prolyl) ameliorates the induced radiation damage is not clear.

Cell Ploidy and Repair Ability Determine the Radioprotective Effects of Cysteamine in Yeast Cells of Various Species and Genotypes

  • Vladislav G. Petin;Kim, Jin-Kyu
    • Korean Journal of Environmental Biology
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    • v.20 no.1
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    • pp.35-39
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    • 2002
  • The significance of cell ploidy and repair ability for the radioprotective efficiency of cysteamine was studied in DNA repair - proficient and repair - deficient yeast cells irradiated $^{60}C0\;\gamma-rays.$ Results have been obtained for the cell survival of two groups of yeasts-diplont and haplont cells, both in haploid and diploid states. For diploid Saccharomyces cerevisiae yeast cells, the correlation between the radio-protective action of cysteamine and the cell repair capacity was demonstrated. Such a correlation was not clearly expressed for haploid yeast cells. In addition, evidence was obtained indicating that the degree of the radioprotective action was independent of the number of chromosome sets in haplont yeast Pichia guilliermondii cells and in some radiosensitive mutants defective in the diploid-specific recovery. It is concluded on this basis that the radioprotective action may involve the cellular recovery process, which may be mediated by a recombination-like mechanism, for which the diploid state is required. The results obtained clearly show that the radioprotective effect was dependent on DNA repair status and indicate that the mechanism of the radioprotective action may be realized on the level of primary radiation damage production as well as on the level of postradiation recovery from potentially lethal radiation damage.

Radioprotective Effect of Saengmaek-san on Mice Jejunal Crypt Cell Survival and Apoptosis (생맥산(生脈散)의 방사선 보호효과 : 생쥐 소낭세포 재생과 Apoptosis에 미치는 영향)

  • Kim, Hyun-Kyung;Yoon, Sang-Hyub;Ryu, Bong-Ha;Kim, Jin-Sung
    • The Journal of Internal Korean Medicine
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    • v.27 no.2
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    • pp.316-326
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    • 2006
  • Backgrounds & Objects: The aim of this study was to investigate the radioprotective effect of Shengmai-san(SMS), a herbal medicine, on mice jejunal crypt cell survival and Apoptosis. Methods: Mice were devided into 4 groups according to radiation dose and SMS treatment: Normal was the group without irradiation. Control was the group treated with D.W before 10 Gy irradiation. SMS 2.9 was sample group treated with 2.9 mg/10 g of SMS extract before 10 Gy irradiation and SMS 29 was sample group treated with 29 mg/10 g of SMS extract before 10 Gy irradiation. And Each group were sacrificedat 24 hours and 72 hours after irradiation. To analyze the crypt survival, hematoxylin-eosin staining was used and to analyze the apoptosis, the TUNEL assay was done. Results: 1. From the microcolony survival assay, the SMS 2.9 and SMS 29 showed the radioprotective effect with a statistical significance compared to the control group at 24 hr (P < 0.01) and 72 hr (p < 0.001) after 10 Gy irradiatien. And the differences of radioprotective effect between SMS 2.9 and SMS 29 were net significant. 2. The results of the TUNEL assay showed that the apoptotic index in SMS 2.9 and SMS 29 was significantly decreased, as compared to the control group at both 24 hr ( p < 0.01) and 72 hr (SMS 2.9 : p < 0.001. SMS 29 : P < 0.01) after 10Gy irradiation And the differences of between SMS 2.9 and SMS 29 were not significant. Conclusions: It could be suggested that the Shengmai-san has a prominent Protective effect in mice intestines against the radiation damage. And the radieprotective effect seems to be related to inhibition of the apoptosis.

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Radioprotective effects of delphinidin on normal human lung cells against proton beam exposure

  • Kim, Hyun Mi;Kim, Suk Hee;Kang, Bo Sun
    • Nutrition Research and Practice
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    • v.12 no.1
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    • pp.41-46
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    • 2018
  • BACKGROUND/OBJECTIVES: Exposure of the normal lung tissue around the cancerous tumor during radiotherapy causes serious side effects such as pneumonitis and pulmonary fibrosis. Radioprotectors used during cancer radiotherapy could protect the patient from side effects induced by radiation injury of the normal tissue. Delphinidin has strong antioxidant properties, and it works as the driving force of a radioprotective effect by scavenging radiation-induced reactive oxygen species (ROS). However, no studies have been conducted on the radioprotective effect of delphinidin against high linear energy transfer radiation. Therefore, this study was undertaken to evaluate the radioprotective effects of delphinidin on human lung cells against a proton beam. MATERIALS/METHODS: Normal human lung cells (HEL 299 cells) were used for in vitro experiments. The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay assessed the cytotoxicity of delphinidin and cell viability. The expression of radiation induced cellular ROS was measured by the 2'-7'-dicholordihydrofluorescein diacetate assay. Superoxide dismutase activity assay and catalase activity assay were used for evaluating the activity of corresponding enzymes. In addition, radioprotective effects on DNA damage-induced cellular apoptosis were evaluated by Western blot assay. RESULTS: Experimental analysis, including cell survival assay, MTT assay, and Western blot assay, revealed the radioprotective effects of delphinidin. These include restoring the activities of antioxidant enzymes of damaged cells, increase in the levels of pro-survival protein, and decrease of pro-apoptosis proteins. The results from different experiments were compatible with each to provide a substantial conclusion. CONCLUSION: Low concentration ($2.5{\mu}M/mL$) of delphinidin administration prior to radiation exposure was radioprotective against a low dose of proton beam exposure. Hence, delphinidin is a promising shielding agent against radiation, protecting the normal tissues around a cancerous tumor, which are unintentionally exposed to low doses of radiation during proton therapy.

Chemical modifying agents of radiation effect (방사선조사(放射線照射)의 생물학적효과(生物學的效果)에 영향을 미치는 약제(藥劑))

  • Han, Man-Chung;Chang, Kee-Hyun
    • Journal of Radiation Protection and Research
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    • v.5 no.1
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    • pp.32-35
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    • 1980
  • A number of chemical compounds that modify radiation effects are reviewed, with brief report of our own experiments on radioprotective effect of some vasoconstrictive agents and 5-Thio-D-Glucose. Sulfhydryl compounds(-SH group) and some pharmacologic compounds such as CNS depresants, vasoconstrictive agents and autonomic drugs are known to have radioprotective effect in experimental research and in limitted clinical study, whereas oxygen, hallogenated pyrimidines and metronidazole, etc. have radiosensitizing effect. Author experimentally observed some radioprotective effects of angiotensin II, a strong vasoconstrictor, and 5-Thio-D-glucose in mice.

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Radioprotective Effect of S-2(3-aminopropylamino)Ethyl Phosphorothioic Acid (WR-2721) on Lipid Metabolism in X-ray irradiated Rats (S-2(3-aminopropylamino)Ethyl Phosphorothioic Acid (WR-2721)가 방사선에 조사된 흰쥐의 지질대사에 미치는 영향)

  • Ko, Seong-Jin;Kim, Jae-Young;Lee, Chun-Bok
    • Journal of radiological science and technology
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    • v.20 no.1
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    • pp.91-96
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    • 1997
  • Male rats of Albino strain were divided into four groups. The radioprotective effect of treatment with S-2(3-aminopropylamino)ethylphosphorothioic acid(WR-2721) using the dose of 200 mg/kg by intraperitonial injection on rats for 20 min prior to whole body x-ray irradiation (8 Gy) was studied. The harzardous effects of x-ray irradiation were greatly corrected In the treated group. The concentrations of total serum cholesterol, HDL-cholesterol, triglyceride, and phospholipid were greatly affected, showing insignificant changes in the treated group of animals. The drastic hyperglycemic effect of x-ray irradiation in the untreated group decreased to a normal level. These results show the potentiality of WR-2721 as a radioprotective agent.

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In Vitro Activity of Cyclic Dipeptides Against Gram-Positive and Gram-Negative Anaerobic Bacteria and Radioprotective Effect on Lung Cells

  • RHEE KI-HYEONG
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.158-162
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    • 2006
  • Cyclic dipeptides isolated from Streptomyces sp. have been shown to have antimicrobial activity as well as other potentially useful biological activities. The purpose of this study was to compare the in vitro activity of two cyclic dipeptides combined against anaerobic bacteria with the activity of other antimicrobial agents. Specifically, the in vitro activity of the combination of two cyclic dipeptides was investigated against 140 clinical isolates of anaerobic bacteria by the agar dilution method and was compared with that of erythromycin, cefoxitin, imipenem, clindamycin, and metronidazole. The cyclic dipeptide combination and imipenem were the most active antimicrobial agents tested. In addition, the cyclic dipeptide combination had a radioprotective effect on five normal human lung fibroblast cells, showing survival rates higher $(>90\%)$ than either of the two cyclic dipeptides alone $(<80\%)$.

RADIOPROTECTIVE EFFECT OF ALGIN-OLIGOSACCHARIDE THROUGH MEASURING CASPASE-3 AND CASPASE-9 IN MICE

  • Choi, Seong-Kwan;Jung, Woon-Kwan;Lee, Kyu-Soo;Jang, Young-Il;Dong, Kyeong-Rae
    • Journal of Radiation Protection and Research
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    • v.32 no.4
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    • pp.134-139
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    • 2007
  • In order to find out the Radioprotective effect of algin-oligosaccharide(AOS), this study, with a mouse of which whole frame irradiated by 3 Gy radiation once, measured caspase-3 and caspase-9 amid cell signaling connected to apoptosis in order to observe cell activation. In Caspase-3 and Caspase-9 test for observing cell activation, both of Caspase-3 and Caspase-9 showed highly increased O.D. value in the irradiation control group, while the whole groups treated with algin-oligosaccharide before or after irradiation indicated lower O.D. value than the irradiation control group, especially showed big difference in 7 day's treatment group of before irradiation (P<0.001). It confirmed that Caspase generation was restrained in AOS treatment group. Consequently, this study inquired into the fact that algin-oligosaccharide with superior antioxidant activity performed radiation protection by inducing restraint of Caspase generation and confirmed that natural product with less chemical toxicity was able to be applied as radioprotector.

A comparative study of radioprotection with Callophyllis japonica extract and amifostine against lethal whole body gamma irradiation in mice

  • Shin, Tae-Kyun;Kim, Hee-Chul;Kim, Jeong-Tae;Ahn, Mee-Jung;Moon, Chang-Jong;Hyun, Jin-Won;Jee, Young-Heun;Lee, Nam-Ho;Park, Jae-Woo
    • Oriental Pharmacy and Experimental Medicine
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    • v.10 no.1
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    • pp.1-6
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    • 2010
  • The efficacy of the radioprotective effect of Callophyllis japonica ethyl acetate (CJEA) extract was studied by comparing it to that of amifostine, a well-known radioprotective agent, and by evaluating the dose reduction factor, an indicator of radioprotective efficacy. Pretreatment with CJEA extract (100 mg/kg body weight) prior to receiving 12 Gy irradiation significantly improved the survival of jejunal crypts at 3.5 day post-irradiation, but attenuated the level of malondialdehyde compared to vehicle alone (P < 0.01). A similar gastroprotective effect was also obtained in the amifostine-treated irradiated group (P < 0.01). The efficacy of the radioprotective effect was further confirmed by the dose reduction factor, 1.41. Collectively, these results suggest that CJEA extract is a useful radioprotectant whose efficacy is similar to that of amifostine and whose radioprotective mechanism is in part the reduction of lipid peroxidation caused by gamma irradiation.

Study for Research Trends on Radioprotective Effects of Herbs (한약의 방사선 부작용 억제효과에 관한 경향 분석)

  • Lee, Soo-Jin
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.4
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    • pp.559-565
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    • 2010
  • Cancer is already a well-recognized main cause of mortality and the incidence of cancer is increasing steadily. Because conventional treatment modalities for cancer accompanies severe side effects, traditional medicine has been considered as alternatives to reduce the adverse effects and its use has continued to rise in cancer therapy. This study aims to summarize and make a reference of radioprotective effects of herbs worldwide. In this process, this review surveyed all papers of radioprotective-focused studies using herbal medicine in PubMed database and finally 44 papers were included. The type of materials, formation of experiments, type of herbal medicine, their action and mechanisms, and type of cancer were analyzed. The number of studies on radioprotective effects of herbal medicine has increased since 2000. The main formation of experiments was clinical study and the portion was 45% and the proportion of the research using prescriptions was 51% and the research using herbal products was 25%. Herbs and prescriptions having the effects of tonifying and nourishment were used the most. Most of herbal medicine in this study can enhance immune function, increase anti-oxidant effect, regulate cell cycle and increase sensitivity to radiotherapy. This study will provide the useful information on development of herbal medicine having radioprotective effects.