• Title, Summary, Keyword: ROS

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Relationship between reactive oxygen species and autophagy in dormant mouse blastocysts during delayed implantation

  • Shin, Hyejin;Choi, Soyoung;Lim, Hyunjung Jade
    • Clinical and Experimental Reproductive Medicine
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    • v.41 no.3
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    • pp.125-131
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    • 2014
  • Objective: Under estrogen deficiency, blastocysts cannot initiate implantation and enter dormancy. Dormant blastocysts live longer in utero than normal blastocysts, and autophagy has been suggested as a mechanism underlying the sustained survival of dormant blastocysts during delayed implantation. Autophagy is a cellular degradation pathway and a central component of the integrated stress response. Reactive oxygen species (ROS) are produced within cells during normal metabolism, but their levels increase dramatically under stressful conditions. We investigated whether heightened autophagy in dormant blastocysts is associated with the increased oxidative stress under the unfavorable condition of delayed implantation. Methods: To visualize ROS production, day 8 (short-term dormancy) and day 20 (long-term dormancy) dormant blastocysts were loaded with $1-{\mu}M$ 5-(and-6)-chloromethyl-2', 7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-$H_2DCFDA$). To block autophagic activation, 3-methyladenine (3-MA) and wortmannin were used in vivo and in vitro, respectively. Results: We observed that ROS production was not significantly affected by the status of dormancy; in other words, both dormant and activated blastocysts showed high levels of ROS. However, ROS production was higher in the dormant blastocysts of the long-term dormancy group than in those of the short-term group. The addition of wortmannin to dormant blastocysts in vitro and 3-MA injection in vivo significantly increased ROS production in the short-term dormant blastocysts. In the long-term dormant blastocysts, ROS levels were not significantly affected by the treatment of the autophagy inhibitor. Conclusion: During delayed implantation, heightened autophagy in dormant blastocysts may be operative as a potential mechanism to reduce oxidative stress. Further, ROS may be one of the potential causes of compromised developmental competence of long-term dormant blastocysts after implantation.

Pathogenesis strategies and regulation of ginsenosides by two species of Ilyonectria in Panax ginseng: power of speciation

  • Farh, Mohamed El-Agamy;Kim, Yu-Jin;Abbai, Ragavendran;Singh, Priyanka;Jung, Ki-Hong;Kim, Yeon-Ju;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.44 no.2
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    • pp.332-340
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    • 2020
  • Background: The valuable medicinal plant Panax ginseng has high pharmaceutical efficacy because it produces ginsenosides. However, its yields decline because of a root-rot disease caused by Ilyonectria mors-panacis. Because species within Ilyonectria showed variable aggressiveness by altering ginsenoside concentrations in inoculated plants, we investigated how such infections might regulate the biosynthesis of ginsenosides and their related signaling molecules. Methods: Two-year-old ginseng seedlings were treated with I. mors-panacis and I. robusta. Roots from infected and pathogen-free plants were harvested at 4 and 16 days after inoculation. We then examined levels or/and expression of genes of ginsenosides, salicylic acid (SA), jasmonic acid (JA), and reactive oxygen species (ROS). We also checked the susceptibility of those pathogens to ROS. Results: Ginsenoside biosynthesis was significantly suppressed and increased in response to infection by I. mors-panacis and I. robusta, respectively. Regulation of JA was significantly higher in I. robusta-infected roots, while levels of SA and ROS were significantly higher in I. mors-panacis-infected roots. Catalase activity was significantly higher in I. robusta-infected roots followed in order by mock roots and those infected by I. mors-panacis. Moreover, I. mors-panacis was resistant to ROS compared with I. robusta. Conclusion: Infection by the weakly aggressive I. robusta led to the upregulation of ginsenoside production and biosynthesis, probably because only a low level of ROS was induced. In contrast, the more aggressive I. mors-panacis suppressed ginsenoside biosynthesis, probably because of higher ROS levels and subsequent induction of programmed cell death pathways. Furthermore, I. mors-panacis may have increased its virulence by resisting the cytotoxicity of ROS.

ROS-based Pick-and-Place Motion Control for a Robot Arm of 4 Degrees of Freedom (자유도-4 로봇 팔을 위한 ROS 기반 Pick-and-Place 동작 제어)

  • Kim, Young-Ju
    • Proceedings of the Korean Society of Computer Information Conference
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    • pp.53-54
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    • 2018
  • 본 논문은 ROS 프레임워크를 기반으로 4-자유도를 가진 로봇 팔의 Pick-and-Place 동작 제어를 구현하고, 틱택토 게임에 적용한 사례를 제시한다. 로봇 팔의 Pick-and-Place 동작 제어는 움직임 궤적 계획, 충돌 회피 그리고 역기구학 모델링 연산들과 이를 이용한 복잡한 제어 과정을 요구한다. ROS 프레임워크는 간단한 인터페이스 통해 로봇 팔의 동작을 용이하게 제어할 수 있도록 일련의 연산들과 제어 동작을 통합하여 MoveIt 패키지를 제공하고 있으며, 본 논문은 이 패키지를 기반으로 4-자유도의 로봇 팔에 대한 동작 제어 모듈을 구현하였다. 또한 이를 틱택토 게임에 적용하여 로봇 팔을 적절히 제어함을 확인하였다.

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Mechanism of Photodynamic Therapy using 9-hydroxypheophorbide-alpha on HeLa Cell Lines

  • Ahn, Jin-Chul
    • Biomedical Science Letters
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    • v.15 no.2
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    • pp.153-160
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    • 2009
  • Photodynamic therapy(PDT) is a treatment utilizing the generation of singlet oxygen and other reactive oxygen species(ROS), which selectively accumulate in target cells. The aim of present work is to investigate the photodynamic therapy mechanism of 9-HpbD-a-mediated PDT in HeLa cell lines. We studied the general reactive oxygen species(G-ROS) activation after 9-HpbD-a PDT using fluorescence stain with $H_2DCF-DA$. G-ROS activation observed after 9-HpbD-a PDT and higher activation condition was 1 hour after PDT at 0.5 ${\mu}g/ml$ 9-HpbD-a concentration. Sodium azide and reduced glutathione(the singlet oxygen quencher) could protect HeLa cells from cell death induced by 9-HpbD-a PDT. But D-mannitol(the hydroxyl radical scavenger) could not protect cell death. Singlet oxygen played a decisive role in 9-HpbD-a PDT induced HeLa cell death. Type II reaction was the main type of ROS formation at 9-HpbD-a PDT.

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The Function of Reactive Oxyegn Species in Bone Loss (골손실을 조절하는 활성산소종의 역할 규명)

  • Yang, Mi-Hye;Park, Hyo-Jung;Lee, Dong-Seok;Yim, Mi-Jung
    • YAKHAK HOEJI
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    • v.53 no.4
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    • pp.179-183
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    • 2009
  • We explored the role of reactive oxygen species (ROS) in LPS-induced bone loss. LPS was shown to increase the concentration of ROS in osteoclast precursors. The antioxidant decreased osteoclast formation by LPS. Furthermore, the antioxidant decreased NFATc1 expression by LPS, suggesting that ROS mediates NFATc1 expression in the regulation of LPS-induced osteoclast formation. Finally, the antioxidant decreased LPS-induced RANKL mRNA expression in osteoblasts. Taken together, these data indicate that LPS mediates ROS to induce bone loss.

Event Port Extension of OPRoS Framework for Inter-connecting with ROS Topic (ROS 토픽과 결합 가능한 OPRoS 프레임워크의 이벤트 포트 확장 개발)

  • Jang, Choulsoo;Song, Byoungyoul;Kim, Sunghoon
    • Journal of Institute of Control, Robotics and Systems
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    • v.20 no.12
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    • pp.1252-1258
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    • 2014
  • ROS is based on a graph architecture where processing takes place in nodes. Nodes communicate together by passing messages through topics based on the publish/subscribe model. On the other hand, OPRoS components know each other and are tightly-coupled via port connections, and different coupling schemes make the interoperation between two platforms difficult. This paper describes an extension of OPRoS framework to support the interoperation with the ROS topic.

Zinc finger and BTB domain-containing protein 3 is essential for the growth of cancer cells

  • Lim, Ji-Hong
    • BMB Reports
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    • v.47 no.7
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    • pp.405-410
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    • 2014
  • ZBTB3 belongs to the Zinc finger and BTB/POZ domain containing transcription factor family; however, its biological role has rarely been studied. We demonstrate for the first time, to our knowledge, that ZBTB3 is an essential factor for cancer cell growth via the regulation of the ROS detoxification pathway. Suppression of ZBTB3 using two different short hairpin RNAs in human melanoma, lung carcinoma, and breast carcinoma results in diminished cell growth. In addition, we found that suppression of ZBTB3 activates a caspase cascade, including caspase-9, -3, and PARP leading to cellular apoptosis, resulting from failed ROS detoxification. We identified that ZBTB3 plays an important role in the gene expression of ROS detoxification enzymes. Our results reveal that ZBTB3 may play a critical role in cancer cell growth via the ROS detoxification system. Therefore, therapeutic strategies that target ZBTB3 could be used in selective cancer treatments.

Thieny/Furanyl-hydroxyphenylpropenones as Inhibitors of LPS-induced ROS and NO Production in RAW 264.7 Macrophages, and Their Structure-Activity Relationship Study

  • Kadayat, Tara Man;Kim, Mi Jin;Nam, Tae-Gyu;Park, Pil-Hoon;Lee, Eung-Seok
    • Bulletin of the Korean Chemical Society
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    • v.35 no.8
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    • pp.2481-2486
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    • 2014
  • Twelve thienyl/furanyl-hydroxyphenylpropenones were systematically designed and synthesized, and evaluated for their inhibitory effect on LPS-induced ROS and NO production in RAW 264.7 macrophages. Compound 11 displayed the most significant inhibitory activity of LPS-induced ROS and NO production in RAW 264.7 macrophages. Structure-activity relationship study indicated that para-hydroxyphenyl moiety plays an important role for inhibitory activities on both LPS-induced ROS and NO production as well as 3-thienyl moiety on molecule.

Design, Synthesis and in-vitro Screening of New 1H-Pyrazole and 1,2-Isoxazole Derivatives as Potential Inhibitors for ROS and MAPK14 Kinases

  • Al-Sanea, Mohammad M.;El-Deeb, Ibrahim M.;Lee, So Ha
    • Bulletin of the Korean Chemical Society
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    • v.34 no.2
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    • pp.437-442
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    • 2013
  • A new series of 4-(2-(substituted)pyridin-4-yl)-3-(3-methoxy-5-methylphenyl)-1H-pyrazoles (4a-f) and their 1,2-isoxazole analogues (5a-f) has been rationally designed, synthesized and screened against both ROS and MAPK14 kinases. Compounds 4b, 4c and 4e showed moderate inhibitions against both ROS and MAPK14 kinases. Compound 4e has showed the strongest inhibitions with IC50 values of 1.25 ${\mu}M$ and 3.00 ${\mu}M$ against ROS and MAPK14 kinases, respectively. A brief structure-activity relationship study and a molecular modeling study were made revealing a group of essential structural features for good kinase inhibitory activity within this new class of kinase inhibitors.

Synthesis and Biological Activity of New 4-(Pyridin-4-yl)-(3-methoxy-5-methylphenyl)-1H-pyrazoles Derivatives as ROS Receptor Tyrosine Kinase Inhibitors

  • Park, Byung Sun;El-Deeb, Ibrahim M.;Yoo, Kyung Ho;Han, Dong Keun;Tae, Jin Sung;Lee, So Ha
    • Bulletin of the Korean Chemical Society
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    • v.33 no.11
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    • pp.3629-3634
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    • 2012
  • A series of new 4-(pyridin-4-yl)-(3-methoxy-5-methylphenyl)-1H-pyrazoles (6a-k & 7a-l) has been rationally designed based on the structure of the lead compound KIST301080, a selective ROS receptor tyrosine kinase inhibitor, in order to study the activity of ROS of this new class of inhibitors. The compounds were synthesized and screened against ROS kinase, where compound 6h showed moderate inhibitory activity with an $IC_{50}$ value of $6.25{\mu}M$. The study emphasized the importance of the acetonitrile group at the pyrazole ring and also the importance of having a hydrogen bond donor on the distal phenyl ring linked to the pyridine moiety.