• 제목, 요약, 키워드: ROS

검색결과 2,220건 처리시간 0.046초

Detection of Mitochondrial Reactive Oxygen Species in Living Rat Trigeminal Caudal Neurons

  • Lee, Hae In;Chun, Sang Woo
    • International Journal of Oral Biology
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    • v.40 no.2
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    • pp.103-109
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    • 2015
  • Growing evidence suggests that mitochondrial reactive oxygen species (ROS) are involved in various pain states. This study was performed to investigate whether ROS-induced changes in neuronal excitability in trigeminal subnucleus caudalis are related to ROS generation in mitochondria. Confocal scanning laser microscopy was used to measure ROS-induced fluorescence intensity in live rat trigeminal caudalis slices. The ROS level increased during the perfusion of malate, a mitochondrial substrate, after loading of 2',7'-dichlorofluorescin diacetate ($H_2DCF-DA$), an indicator of the intracellular ROS; the ROS level recovered to the control condition after washout. When pre-treated with phenyl N-tert-butylnitrone (PBN) and 4-hydroxy-2,2,6,6-tetramethylpiperidene-1-oxyl (TEMPOL), malate-induced increase of ROS level was suppressed. To identify the direct relation between elevated ROS levels and mitochondria, we applied the malate after double-loading of $H_2DCF-DA$ and chloromethyl-X-rosamine (CMXRos; MitoTracker Red), which is a mitochondria-specific fluorescent probe. As a result, increase of both intracellular ROS and mitochondrial ROS were observed simultaneously. This study demonstrated that elevated ROS in trigeminal subnucleus caudalis neuron can be induced through mitochondrial-ROS pathway, primarily by the leakage of ROS from the mitochondrial electron transport chain.

The reactive oxygen species-total antioxidant capacity score is a new measure of oxidative stress to predict male infertility

  • Sharma, Rakesh K.;Pasqualotto, Fabio F.;Nelson, David R.;Thomas Jr, Anthony J.;Agarwal, Ashok
    • 대한생식의학회:학술대회논문집
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    • pp.29-35
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    • 2000
  • The imbalance between reactive oxygen species (ROS) production and total antioxidant capacity (TAC) in seminal fluid indicates oxidative stress and is correlated with male infertility. A composite ROS-TAC score may be more strongly correlated with infertility than ROS or TAC alone. We measured ROS, TAC, and ROS-TAC scores in semen from 127 patients and 24 healthy controls. Of the patients, 56 had varicocele, eight had varicocele with prostatitis, 35 had vasectomy reversals, and 28 had Idiopathic infertility. ROS levels were higher among infertile men, especially those with varicocele with prostatitis (mean ${\pm}$ SE, 3.25 ${\pm}$ 0.89) and vasectomy reversals (2.65 ${\pm}$ 1.01). All infertility groups had significantly lower ROS-TAC scores than control. ROS-TAC score identified 80% of patients and was significantly better than ROS at identifying varicocele and idiopathic infertility. The 13 patients whose partners later achieved pregnancies had a mean ROS-TAC score of 47.7 ${\pm}$ 13.2, similar to controls but significantly higher than the 39 patients who remained infertile (35.8 ${\pm}$ 15.0; P < 0.01). ROS-TAC score is a novel measure of oxidative stress and Is superior to ROS or TAC alone in discriminating between fertile and infertile men. Infertile men with male factor or idiopathic diagnoses had significantly lower ROS-TAC scores than controls, and men with male factor diagnoses that eventually were able to initiate a successful pregnancy had significantly higher ROS-TAC scores than those who failed.

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Rare Incidence of ROS1 Rearrangement in Cholangiocarcinoma

  • Lim, Sun Min;Yoo, Jeong Eun;Lim, Kiat Hon;Tai, David Wai Meng;Cho, Byoung Chul;Park, Young Nyun
    • Cancer Research and Treatment
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    • v.49 no.1
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    • pp.185-192
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    • 2017
  • Purpose The recent discovery and characterization of an oncogenic ROS1 gene rearrangement has raised significant interest because small molecule inhibitors are effective in these tumors. The aim of this study was to determine frequency and clinicopathological features associated with ROS1 rearrangement in patients with cholangiocarcinoma (CCA). Materials and Methods A total of 261 patients who underwent surgery for CCA between October 1997 and August 2013 were identified from an international, multi-institutional database. ROS1 rearrangement was evaluated by break-apart fluorescence in situ hybridization using tissue microarrays of these patients. Results Of 261 CCA evaluated, three cases (1.1%) showed ROS1 rearrangement by fluorescence in situ hybridization (FISH), all of which were derived from intrahepatic origin. ROS1 protein expression was observed in 38 samples (19.1%). Significantly larger tumor size was observed in ROS1 immunohistochemistry (IHC)-negative patients compared with ROS1 IHC-positive patients. ROS1 FISH-positive patients had a single tumor with a median size of 4 cm and well-to-moderate differentiation. Overall, there was no difference in terms of baseline characteristics, overall survival, and recurrence-free survival between ROS1-positive and -negative patients. Conclusion ROS1 rearrangement was detected in 1.1% of CCA patients. Although rare, conduct of clinical trials using ROS1 inhibitors in these genetically unique patients is warranted.

활성산소종으로 손상된 혈관내피세포에 대한 Vitamin E의 항산화 효과 (Antioxidant Effect of Vitamin E on Vascular Endothelial Cells Damaged by Reactive Oxygen Species)

  • 석승한
    • 동의생리병리학회지
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    • v.20 no.3
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    • pp.685-689
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    • 2006
  • In order to examine the injury of vascular endothelial cells related with oxidative stress of reactive oxygen species(ROS), mophological changes of vascular endothelial cells were observed by light microscope after bovine pulmonary vascular endothelial cell line (BPVEC) was treated with 15 uM of hydrogen peroxide. In addition, the effect of vitamin E against ROS-induced oxidative stress was examined by light microscope. In this study, the cell number of BPVEC treated with ROS has significantly decreased than that of control, and the loss of cytoplasmic processes and cell swelling were observed in BPVEC treated with ROS. Whereas, cell number of BPVEC treated with vitamin E has significantly increased than that of BPVEC treated with ROS and also, cytoplasmic processes of BPVEC treated with vitamin E were preserved as control. These findings suggested that not only did ROS induce damage of BPVES by decrease of cell number, loss of cytoplasmic processes and cell swelling, but vitamin E also has protective effect against ROS-induced oxidative stress in cultures of BPVEC.

비타민 C와 글루타치온이 수은유도 ROS 생성에 미치는 영향 (Effect of Vitamin C and GSH on the Hg Induced ROS)

  • 권경진;신윤용
    • Environmental health and toxicology
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    • v.23 no.1
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    • pp.33-39
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    • 2008
  • The genotoxicity of mercury compounds have been investigated with a variety of genetic endpoints in prokaryotic and eukaryotic cells. The mercury ions are positively charged and easily form complexes with DNA by binding with negatively charged centers to cause mutagenesis. Further, the mercury ions can react with sulfhydryl (-SH) groups of proteins associated with DNA replication and alter genetic information. Another mechanism by which mercury damages DNA molecule is via its probable involvement of reactive oxygen species (ROS) and induces DNA strand breaks. In order to investigate whether the ROS production was induced by mercury, we performed ROS assay. As the result, the ROS production was significantly increased when it grows dose-dependently and time-dependently. We compared mercury alone-treated group and mercury co-treated with Vitamin C or glutathione group. As the result, the ROS production induced by mercury was decreased by Vitamin C and glutathione. Co-treated with Vitamin C and glutathione group was the most effective to lowering ROS production induced by mercury.

OPRoS-ROS간 데이터 교환 및 서비스 호출을 위한 브리지 (Bridge for Exchange of Data and Service Invocation Between OPRoS and ROS)

  • 이기원;박홍성
    • 제어로봇시스템학회논문지
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    • v.22 no.2
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    • pp.153-161
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    • 2016
  • This paper proposes a bridge model for data exchange and service invocation between OPRoS and ROS platforms, shows the validity of the proposed model via applications, and compares the proposed model with the OPRoS platform and the ROS platform using performance measures such as data exchange time and service response time. The proposed model operates independently of OPRoS and ROS Platforms using its configuration file with mapping information among the OPRoS data/service port and the ROS topic/service. The configuration file makes easy connections between OPRoS data/service and ROS topic/service without changing the source code of the platform and components.

참산부추(Allium sacculiferum Max.) 메탄올 추출물의 지방세포 내 ROS 생성 및 지질 축적 억제 효능 (Inhibitory Effects of Allium sacculiferum Max. Methanol Extracts on ROS Production and Lipid Accumulation during Differentiation of 3T3-L1 Cells)

  • 최혜영;김건희
    • 한국식품영양과학회지
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    • v.43 no.6
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    • pp.822-828
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    • 2014
  • 본 연구에서는 3T3-L1 지방전구세포를 이용하여 Allium 속 식물의 하나인 참산부추(ASM) 메탄올 추출물의 ROS 생성 저해 및 지질 축적 억제 효과를 확인하고자 하였다. 먼저 ASM 메탄올 추출물 $100{\sim}2,000{\mu}g/mL$의 모든 농도에서 유의적인 DPPH 라디칼 소거능을 나타내었으며, 지방전구세포에 ASM 메탄올 추출물 $10{\sim}100{\mu}g/mL$를 처리하였을 때 세포 독성을 나타내지 않았다. 지방세포 내 ROS 관련 효소와 분화 관련 전사인자의 조절로 인한 중성지방 축적 저해 활성을 확인하기 위하여 지방전구세포를 분화 유도하면서 추출물을 농도별(10, 50, $100{\mu}g/mL$)로 처리하였다. 그 결과 ASM 메탄올 추출물은 대조군에 비해 ROS 생성량과 ROS 관련 효소인 G6PDH mRNA 발현을 농도 의존적으로 감소시켰다. 또한 ASM 메탄올 추출물에 의하여 지방세포 내 중성지방 축적량이 유의적으로 감소하였으며, 지방세포 분화에 관련된 전사인자인 SREBP1c, $PPAR{\gamma}$$C/EBP{\alpha}$ mRNA 발현도 유의적으로 감소하였다. 이상의 결과들로 볼 때 ASM 메탄올 추출물로 인한 ROS 생성 저해와 지질 축적 억제는 ROS 생성 및 ROS 관련 유전자의 발현감소로 인한 지방 생성 주요 전사인자의 유전자 발현 억제로 인한 것으로 보이며, ASM이 항비만 효과가 있는 천연물 소재로 개발 가능성이 있을 것으로 사료된다.

Inhibitory Effect of Probenecid on Osteoclast Formation via JNK, ROS and COX-2

  • Cheng, Mi Hyun;Kim, Sung-Jin
    • Biomolecules & Therapeutics
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    • v.28 no.1
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    • pp.104-109
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    • 2020
  • Probenecid is a representative drug used in the treatment of gout. A recent study showed that probenecid effectively inhibits oxidative stress in neural cells. In the present study, we investigated whether probenecid can affect osteoclast formation through the inhibition of reactive oxygen species (ROS) formation in RAW264.7 cells. Lipopolysaccharide (LPS)-induced ROS levels were dose-dependently reduced by probenecid. Fluorescence microscopy analysis clearly showed that probenecid inhibits the generation of ROS. Western blot analysis indicated that probenecid affects two downstream signaling molecules of ROS, cyclooxygenase 2 (COX-2) and c-Jun N-terminal kinase (JNK). These results indicate that probenecid inhibits ROS generation and exerts antiosteoclastogenic activity by inhibiting the COX-2 and JNK pathways. These results suggest that probenecid could potentially be used as a therapeutic agent to prevent bone resorption.

허용변화한계법(Limits of Acceptable Change: LAC)과 휴양기회분포(Recreation Opportunity Spectrum: ROS)를 적용한 갯벌자원 평가 (Evaluation of Wetland as Application on Limits of Acceptable Change: LAC.Recreation Opportunity Spectrum: ROS Methods)

  • 김진선
    • 한국조경학회지
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    • v.31 no.4
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    • pp.57-66
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    • 2003
  • The purpose of this study is to evaluate the resource value of existing mud flats in JANGWHARI, KANGWHADO, as strategies for ecotourism. The research methods used in this study are Limits of Acceptable Change(LAC), and Recreation Opportunity Spectrum(ROS). The LAC process draws attention to the existing area conditions that are judged to be acceptable. Managers must define desired area conditions and undertake actions to maintain or achieve these conditions. The ROS is within each of the recreation opportunity classes identified as being used at the regional level. The Results of this study are as follows: 1) The Opportunity Class of the ROS is ecological, physical, social, managerial setting as primitive, semi-primitive. non-motorized, semi-primitive$.$motorized, and roaded natural. 2) The indicator of the LAC is ecological, physical, social, and managerial setting; the indicator of ecological is wildlife populations, water quality, road paving; the indicator of physical is facilities; the indicator of social is visitor needs for knowledge, adventure, eco-experience, and environmental education programs; and, the indicator of managerial is limits of law, and degree of management. 3) Currently, the Opportunity Class of the ROS of JANGWHARI, KANGWHADO is levels II-III, and the Opportunity Class of the suggested ROS is levels I-II. 4) This paper describes strategies for mud flat area management: detection of water quality, resolving problems of equipment, supply of both environmental education programs and guide equipment.

Effects of Protein Kinase Inhibitors on Histamine Release and ROS Generation in RBL 2H3 Cells

  • Yoon, Mi-Yun;Cho, Nam-Young;Lee, Ji-Yun;Seo, Moo-Hyun;Kim, Chang-Jong;Sim, Sang-Soo
    • 대한약학회:학술대회논문집
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    • pp.297.2-297
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    • 2002
  • Previous report showed that histamine release by HCI was mediated via reactive oxygen species (ROS) generation in RBL 2H3 cells. To investigate action of protein kinase on histamine release and ROS generation. we observed effects of protein kinase inhibitors on histamine release and ROS generation in RBL 2H3 cells stimulated by HCI HCI dose-dependently increased both histamine release and ROS generation. HCI-induced histamine release was significantly inhibited by bisindolmaleimide (10 ${\mu}$M). DHC (10 ${\mu}$M). , and wortmannin (10 ${\mu}$M), but not by PD098059 (10 ${\mu}$M). ON the other hand. HCI-induced ROS generation was significantly inhibited by DHC (10 ${\mu}$M). but not by bisindolmaleimide(10 ${\mu}$M). wortmannin (10 ${\mu}$M). and PD098059 (10 ${\mu}$M). However KN-62 did not inhibited both. These results showed that involvement of protein kinase in regulation of histamine release and ROS generation may be different and only tyrosine kinase may be associated with regulation of both histamine release and ROS generation in RBL 2H3 cells.

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