• Title, Summary, Keyword: Pyrene

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A Study on the Degradation of PAH in Organic and Aqueous Phases by Ozone (다환방향족탄화수소에 대한 오존처리의 방법에 관한 연구)

  • Choi, Young Ik;Son, Hee-Jong;Jung, Chul-Woo
    • Journal of Korean Society on Water Environment
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    • v.22 no.6
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    • pp.1123-1129
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    • 2006
  • Pyrene of natural and anthropogenic sources is one of the toxic, mutagenic polycyclic aromatic hydrocarbons (PAHs) listed as priority pollutants. The objectives of this research are to break down pyrene by using ozonation, identify the intermediates and byproducts of pyrene, and test the biodegradability of intermediates and byproducts of pyrene in the aqueous phase. Since pyrene is non-polar, hexane was chosen as a solvent to effectively dissolve pyrene. Pyrene solutions were treated with ozone, as it has high oxidation capacity and electrophilic characteristic. After different ozonation pretreatment times (2, 3, and 10 minutes), intermediates of pyrene in the form of yellowish solid were collected from the hexane solution using a centrifuge. They were identified by gas chromatography/mass spectrometer (GC/MS). $BOD_5$, COD, and E-coli toxicity tests have been performed to assess the ozonation products.

Biodegradation of Pyrene in Marine Environment (해양환경에서 Pyrene의 생분해)

  • 황순석;송홍규
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.53-60
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    • 1999
  • The biodegradation of recalcitrant polycyclic aromatic hydrocarbon, pyrene was investigated in microcosm simulating the beach sand and seawater. The natural biodegradation rates of pyrene were between 30-2,200 ng/g(ml)/day in beach sand and seawater when the pyrenc loading rates were 100- 1,000 ppm at 5-$20^{\circ}C$. The effects of the inoculum size, pyrene concentralion, incubation temperature and surfactant addition were investigated in fertilized (Inipol EAP 22) samples. Generally the biodegradation in beach sand was higher than that in seawater. A mixed inoculum (Pseudomonus, Acinetobacter, Moruxella) showed the 3,120 nglglday of biodegradation rate in beach sand with 200 ppm pyrene, which was 7.8 times higher than the natural biodegradation rate. The highest transformation rate, 4,860 ng/g/day was obtained in the bioaugmented beach sand (1,000 ppm pyrene). The glucose and surfactant addition to enhance the removal have negatively influenced on the biodegradation of pyrene. In case ol surfactants, CMC (critical micell concentration) might bc the control factor for the biodegradation.

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Comparison of Biodegradation of pyrene between Rhizosphere Soil and Non-rhizosphere Soil (Rhizosphere 토양과 Non-rhizosphere 토양에서 Pyrene의 분해속도 비교)

  • 김상채;이의상;서성규
    • Journal of Korea Soil Environment Society
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    • v.3 no.2
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    • pp.71-78
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    • 1998
  • Pyrene is a common petroleum contaminant. This compound is recalcitrant to biological degradation and persists long in contaminated environments. A microcosm experiment was conducted to investigate the degradation rate of pyrene in three different of soil : rhizosphere soil ; non-rhizosphere soil ; and sterilized soil. The degradation rate followed the order of rhizosphere soil)non-rhizosphere soil)sterilized soil. And the rate did not change significantly when organic acids commonly found in the rhizosphere were added to each soil but it seemed to be well related to the increase of the number of microorganisms. Overall, it appears that pyrene is degraded faster in the rhizosphere soil which has the higher microorganism density.

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Novel Electroluminescent Polymer Derived from Pyrene-Functionalized Polyaniline

  • Amarnath, Chellachamy Anbalagan;Kim, Hyoung-Kun;Yi, Dong-Kee;Lee, Sang-Hyup;Do, Young-Rag;Paik, Un-Gyu
    • Bulletin of the Korean Chemical Society
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    • v.32 no.5
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    • pp.1495-1499
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    • 2011
  • A solution processable polymer was synthesized, by incorporating pyrene groups into the backbone of the polyaniline chain, and used as an emissive layer in an organic light emitting diode. The polyaniline base was reacted with acid chloride of pyrene butyric acid to form pyrene-functionalized polyaniline chains. The source of pyrene moiety was acid chloride of pyrene butyric acid. The formation of polymer from acid chloride of pyrene butyric acid and polyaniline was confirmed by the FTIR and $^1H$-NMR spectroscopy. Differential scanning calorimetry revealed high glass transition temperature of 210 $^{\circ}C$. Due to the presence of pyrene moieties in the backbone, the polyaniline synthesized in the present study is solution processable with light emitting property. The photoluminescence spectrum of the polymer revealed that emission lies in the blue region, with a peak at 475 nm. The light emitting device of this polymer exhibits the turn-on voltage of 15 V.

Antimutagenic Effect of Organic Germanium(GE-132) on the Mutagenicity of Benzo(a)pyrene (Benzo(a)pyrene의 돌연변이원성에 대한 유기게르마늄(GE-132)의 항돌연변이 효과)

  • Lee, Hyo-Min;Chung, Yong;Jung, Ki-Wha;Kim, Jae-Wan;Kwon, Sun-Kyung
    • YAKHAK HOEJI
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    • v.37 no.1
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    • pp.18-29
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    • 1993
  • This study was initiated to investigate the effective action and mechanism of GE-132 (Carboxyethylgermanium sesquioxide)on benzo(a)pyrene, which have strong carcinogenicity and mutagenicity. To confirm desmutagenic effect (inhibition of metabolic processes of benzo(a)pyrene with S9 Mix or inactivation of the mutagenicity of benzo(a)pyrene metabolites) and antimutagenic effect (inhibition of gene-expression of reverted genes) of GE-132 against benzo(a)pyrene using with Salmonella typhimuyium TA98 Ames test was performed. The revertants in desmutagenicity test were decreased significantly in the combined groups of benzo(a)pyrene and GE-132 than benzo(a)pyrene only, without inhibition the metabolism of benzo(a)pyrene by S9 Mix. The ideal combined groups of benzo(a)pyrene and GE-132 were 10 $\mu{M}$ and 10mg, 20 $\mu{M}$ and 20mg, 100 $\mu{M}$ and 30 mg, respectively. Then, the revertants in antimutagenicity test, which was studied the direct action of GE-132 on the induction of revertant cells by Salmonella typhimurium TA98 and activated benzo(a)pyrene were decreased significantly in the treated groups of GE-132 than no treated groups. The number of revertants of Salmonella typhimurium TA98 were reduced with increasing amounts of GE-132. From the above results, it was found that GE-132 inactivated the mutagenic metabolites of benzo(a)pyrene without inhibition of the enzyme action in the S9 Mix, and GE132 showed antimutagenic effect which have inhibitory action of reverted gene expression.

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Use of the Comet Assay to Assess DNA Damage in Hemocytes and Gill of Oyster(Crassostrea gigas) Exposed to Pyrene and Benzo(a)pyrene (Pyrene과 Benzo(a)pyrene에 노출된 굴의 혈구세포과 아가미 세포에서의 DNA손상 측정을 위한 Comet assay의 이용)

  • 김기범;배세진
    • Journal of Aquaculture
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    • v.16 no.3
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    • pp.196-201
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    • 2003
  • Sessile organisms such as the oyster Crassostrea gigas have been given much attention as a potential biomonitoring indicator to assess the impact of toxicants on aquatic organism. In this study, we exposed cells isolated from gill of oyster (Crassostrea gigas) to hydrogen peroxide in vitro. In addition oysters were in vivo exposed to pyrene and benzo(a)pyrene at various concentrations for 2 weeks. Comet assay was used to detect DNA single strand breaks and to investigate the application of this technique as a tool for aquatic biomonitoring. Hydrogen peroxide increased DNA single strand break with increasing concentration after 30 minutes exposure in vitro. Pyrene and benzo(a)pyrene caused DNA damage only at very high concentration (100 $\mu\textrm{g}$/L or 1000 $\mu\textrm{g}$/L) at two week exposure in vivo. DNA damage was relatively higher at hemocyte than at gill. It suggested that metabolized PAHs are transferred to hemolymph from digestive gland which have a relatively high enzyme activity, and attacked the DNA of hemocyte, while gill accumulated PAHs without degrading them to their metabolites due to low enzyme activity at gill. Both in vitro and in vivo exposure experiments showed that the comet assay is an effective tool on screening whether the organism are exposed to genotoxic contaminants.

Photodegradation of Pyrene, Chrysene and Benzo[a]pyrene in Water (I) (수중의 Pyrene, Chrysene 및 Benzo[a]pyrene의 광분해(I))

  • 김지용;허철구;이민규;감상규
    • Journal of Environmental Science International
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    • v.12 no.3
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    • pp.337-344
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    • 2003
  • The photodegradation of pyrene, chrysene and benzo[a]pyrene, that were similar in structure among poly-cyclic aromatic hydrocarbons (PAMs), were investigated in water irradiated with a low-pressure mercury lamp (wavelength of 253.7 nm and UV output of 1.35 ${\times}$ 10$\^$-3/J/s). The effects of several factors (t-BuOH, HCO$_3$$\^$-/ and pH) on photodegradation of above three PAHs were also examined. The photodegradation rates of PAHs decreased with increasing the concentration of t-BuOH, but decreased little with increasing the concentration of HCO$_3$$\^$-/ under the concentrations used in this study. The photodegradation rates of PAHs decreased with increasing pH, but their change were greater in case of pH increase from acid to neutral and were little in case of pH increase from neutral to base. The photodegradation rates of PAMs fitted a first-order kinetic model and their photodegradation rates decreased in the following sequences: pyrene>chrysene>benzo[a]pyrene among the PAHs used.

Photodegradation of Pyrene, Chrysene and Benzo[a]pyrene in Water (II) (수중의 Pyrene, Chrysene 및 Benzo[a]pyrene의 광분해(II))

  • 감상규;김지용;주창식;이민규
    • Journal of Environmental Science International
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    • v.12 no.7
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    • pp.775-782
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    • 2003
  • The photodegradations of pyrene, chrysene and benzo[a]pyrene that were similar in structure among polycyclic aromatic hydrocarbons (PAHs) were investigated with a low-pressure mercury lamp(the wavelength of 253.7 nm and UV output of 1.35${\times}$10$\^$-3/J/s). The optimum concentrations of TiO$_2$ and H$_2$O$_2$ on the photodegradation of pyrene, chrysene and benzo[a]pyrene were 1 g/L and 1.5${\times}$10$\^$-3/ M, respectively. By these optimum concentrations, their rates increased with increasing the concentration of TiO$_2$ and H$_2$O$_2$ because the amounts of OH radical formed increased, but for the higher concentrations than the optimum, their rates decreased with increasing those concentrations because the white turbidity phenomena occurs in case of TiO$_2$ and H$_2$O$_2$ acts as an OH radical inhibitor. The photodegradation rates among the photodegradation processes such as UV, UV/TiO$_2$, UV/H$_2$O$_2$, and UV/H$_2$O$_2$/TiO$_2$ decreased in the following sequences.: UV/H$_2$O$_2$/TiO$_2$> UV/H$_2$O$_2$> UV/TiO$_2$> UV.

Synthesis and Property of Pyrene-Naphthalene Diimide-Pyrene Triad (Pyrene-Naphthalene Diimide-Pyrene Triad의 합성 및 물성에 대한 연구)

  • Kim, Hyunji;Kim, A-Rong;Park, Jong S.
    • Textile Coloration and Finishing
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    • v.26 no.4
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    • pp.305-310
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    • 2014
  • In this study, we presented a newly synthesized pyrene-naphthalene diimide(NDI)-pyrene triad. The optical and structural properties were examined using various characterization techniques. A donor-acceptor-donor triad molecule exhibited a strong charge transfer, though there existed neither intramolecular nor intermolecular hydrogen bonding sites, due to the formation of preferential complementary complex between pyrene and NDI. Powder XRD measurement revealed a sharp and distinctive X-ray patterns, indicating the presence of microcrystalline-like structure. POM images showed anisotropic fingerprint texture similar to that of cholesteric phase, and SEM images showed numerous columnar structures with length of 1 to $10{\mu}m$. Above observation clearly demonstrated that ${\pi}$-complementary NDI-pyrene interactions in the traid was strong enough to form columnar aggregates in the long range.

Effects of Ginseng Saponin on DNA Strand Breaks and Replication Inhibition by Benzo(a)Pyrene in CHO-Kl Cells (Benzo(a)Pyrene 유발 DNA 상해 및 복제 억제에 미치는 인삼사포닌의 영향)

  • Park, Jin-Kyu;Park, Ki-Hyun
    • Journal of Ginseng Research
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    • v.16 no.3
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    • pp.210-216
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    • 1992
  • The effect of saponin extracted from Panax grneng CA Meyer on DNA repair and replicative DNA synthesis were examined in CHO-Kl cells cotreated with benzo(a)pyrene and rat liver S-15 fraction. The DNA strand breaks inititated by benzo(a)pyrene metabolites were measured by alkaline election technique. The addition of ginseng saponin to the culture media resulted in decrease of benzo(a)pyrene-induced DNA strand breaks, and restored the suppressed-semiconservative-DNA-synthesis by the carcinogen. DNA repair synthesis in the damaged cells was also elevated by the ginseng treatment when the repairing activites were measured for the (3H)-thymidine incorporation into the carcinogen damaged cellular DNk Comparative analysis of DNA-adduces of benzo(a)pyrene metabortes in microsomes suggested that ginseng saponin treatment in rats reduced the formation of electrophilic metabolites of benzo (a)-pyrene in the rat liver microsomes.

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