• Title/Summary/Keyword: Pseudomonas sp

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Effects of Virious Plant Growth Promoting Rhizobacteria on the Growth of Hydroponically Grown Cucumber Plants in Rockwool and Cocopeat Culture (수종의 식물생장촉진 근권세균이 암면과 코코피트경 오이의 생장에 미치는 영향)

  • Cho, Ja-Yong;Chi, Yeon-Tae;Chung, Soon-Ju
    • Korean Journal of Organic Agriculture
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    • v.7 no.1
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    • pp.105-113
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    • 1998
  • This study was conducted to clarify the effects of various rhizobacteria in the root zone in terms of Azospirillum sp., Rhodopseudomonas sp., Pseudomonas sp., fusant of Bacillus sp. and Corynebacterium glutamicum on the growth of hydroponically grown cucumber plants. Densities in bacterial cells of fusant of Bacillus sp. and Corynebacterium glutamicum at different substrates were in the order of cocopeat > rockwool > nutrient solutions at 4 days after bacterialization. Plant growth promoting effects of the various rhizobacteria on the growth of hydroponically grown cucumber plants were in the order of Azospirillum sp. > Rhodopseudomonas sp. $\ge$ fusant of Bacillus sp. and Corynebacterium glutamicum > Pseudomonas sp. > control.

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Isolation of a Pseudomonas sp. Capable of Utilizing 4-Nonylphenol in the Presence of Phenol

  • Chakraborty Joydeep;Dutta Tapan K.
    • Journal of Microbiology and Biotechnology
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    • v.16 no.11
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    • pp.1740-1746
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    • 2006
  • Enrichment techniques led to the isolation of a Pseudomonas sp. strain P2 from municipal waste-contaminated soil sample, which could utilize different isomers of a commercial mixture of 4-nonylphenol when grown in the presence of phenol. The isolate was identified as Pseudomonas sp., based on the morphological, nutritional, and biochemical characteristics and 16S rDNA sequence analysis. The ${\beta}$-ketoadipate pathway was found to be involved in the degradation of phenol by Pseudomonas sp. strain P2. Gas chromatography-mass spectrometric analysis of the culture media indicated degradation of various major isomers of 4-nonylphenol in the range of 29-50%. However, the selected ion monitoring mode of analysis of biodegraded products of 4-nonylphenol indicated the absence of any aromatic compounds other than those of the isomers of 4-nonylphenol. Moreover, Pseudomonas sp. strain P2 was incapable of utilizing various alkanes individually as sole carbon source, whereas the degradation of 4-nonylphenol was observed only when the test organism was induced with phenol, suggesting that the degradation of 4-nonylphenol was possibly initiated from the phenolic moiety of the molecule, but not from the alkyl side-chain.

Plasmid-Mediated Arsenical and Antimonial Resistance Determinants (ars) of Pseudomonas sp. KM20

  • Yoon, Kyung-Pyo
    • Journal of Microbiology and Biotechnology
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    • v.12 no.1
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    • pp.31-38
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    • 2002
  • Bacteria have evolved various types of resistance mechanism to toxic heavy metals, such as arsenic and antimony. An arsenical and antimonial resistant bacterium was isolated from a shallow creek draining a coal-mining area near Taebaek City, in Kangwon-Do, Korea. The isolated bacterium was identified and named as Pseudomonas sp. KM20 after biochemical and physiological studies were conducted. A plasmid was identified and its function was studied. Original cells harboring the plasmid were able to grow in the presence of 15 mM sodium arsenite, while the plasmid-cured (plasmidless) strain was sensitive to as little as 0.5 mM sodium arsenate. These results indicated that the plasmid of Pseudomonas sp. KM20 does indeed encode the arsenic resistance determinant. In growth experiments, prior exposure to 0.1 mM arsenate allowed immediate growth when they were challenged with 5 mM arsenate, 5 mM arsenite, or 0.1 mM antimonite. These results suggested that the arsenate, arsenite, and antimonite resistance determinants of Pseudomonas sp. KM20 plasmid were indeed inducible. When induced, plasmid-bearing resistance cells showed a decreased accumulation $of\;73^As$ and showed an enhanced efflux $of\;^73As$. These results suggested that plasmid encoded a transport system that extruded the toxic metalloids, resulting in the lowering of the intracellular concentration of toxic oxyanion. In a Southern blot study, hybridization with an E. coli R773 arsA-specific probe strongly suggested the absence of an arsA cistron in the plasmid-associated arsenical and antimonial resistance determinant of Pseudomonas sp. KM20.

Cloning of pcb Genes in Pseudomonas sp.P20 Specifying Degradation of 4-Clorobiphenyl (4-Chlorobiphenyl을 분해하는 Pseudomonas sp. P20의 pcb 유전자군의 클로닝)

  • 남정현;김치경
    • Microbiology and Biotechnology Letters
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    • v.22 no.4
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    • pp.353-359
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    • 1994
  • Pseudomonas sp. P20 was a bacterial isolate which has the ability to degrade 4-chlorobi- phenyl(4CB) to 4-chlorobenzoic acid via the process of meta-cleavage. The recombinant plasmid pCK1 was constructed by insetting the 14-kb EcoRI fragment of the chromosomal DNA containing the 4CB-degrading genes into the vector pBluescript SK(+). Subsequently, E. coli XL1-Blue was transformed with the hybrid plasmid producing the recombinant E. coli CK1. The recombinant cells degraded 4CB and 2,3-dihydroxybiphenyl(2,3-DHBP) by the pcbAB and pcbCD gene products, respectively. The pcbC gene was expressed most abundantly at the late exponential phase in E. coli CK1 as well as in Pseudomonas sp. P20, and the level of the pcbC gene product, 2,3-DHBP dioxygenase, expressed in E. coli CK1 was about two-times higher than in Pseudomonas sp. P20. The activities of 2,3-DHBP dioxygenase on catechol and 3-methylcatechol were about 26 to 31% of its activity on 2,3-DHBP, but the enzyme did not reveal any activities on 4-methylcatechol and 4-chlorocatechol.

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Isolation and Characterization of Diesel Oil Degrading Bacterium, Pseudomonas sp. GENECO 1 Isolated from Oil Contaminated Soil (유류 오염 토양으로부터 분리한 디젤 분해 세균 Pseudomonas sp. GENECO 1의 분리 및 특성 규명)

  • 이종광;김무훈;박형수
    • Korean Journal of Microbiology
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    • v.39 no.2
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    • pp.102-107
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    • 2003
  • With the enrichment culture technique, bacterial strains which degrade diesel oil were isolated from soil contaminated with diesel oil. One of the isolates named GENECO 1 showed the highest activity for emulsification of diesel oil as well as the highest growth rate. This strain, GENECO 1, was identified as a Pseudomonas sp. based on its biochemical, physiological characteristics and 16S rDNA sequences. The optimal cultural conditions for cell growth and oil emulsifying activity of its culture were as follow; $30^{\circ}C$ for temperature, 7.0 for pH. Diesel oil degradation was analysed by the gas chromatography. More than 95% of 1% treated diesel oil were converted into a form no longer extractable by mixed organic solvents after 96 hours incubation.

Characteristics of Biosurfactant Produced by Pseudomonas sp. G314 (Pseudomonas sp. G314가 생산하는 생물 계면활성제의 특성)

  • Shim, So-Hee;Park, Kyeong-Ryang
    • Journal of Life Science
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    • v.22 no.2
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    • pp.239-244
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    • 2012
  • The purpose of this paper is to analyze the characteristics and chemical components of biosurfactant produced by Pseudomonas sp. G314. Pseudomonas sp. G314 was isolated from soil samples which were contaminated with oil in Daejon area. As such, it produced quality biosurfactant [23]. One type of biosurfactant was kept in a refrigerator, whereas another type of biosurfactant was kept in room temperature. The surface tension activities were then compared. As a result, the biosurfactant from Pseudomonas sp. G314 that was kept at room temperature was stable for 10 days, showing 26.2 dyne/cm of surface tension activity. This result was found to be similar to that of the refrigerator storage. The surface tension of batch culture was 25 dyne/cm, but the culture in the 5 l fermentor was 27 dyne/cm. Therefore, it can be suggested that the large-scale culture is feasible via the fermentor. Biosurfactant from Pseudomonas sp. G314 was estimated to be a kind of glycolipid because it dissolved in acetone and methanol much better than in benzene and toluene [23]. A spot was detected through the elution of silica gel column and the spread of TLC, and the Rf value was 0.58. This spot has a positive reaction with Bail's reagent and rhodamine 6G. Hence, we can conclude that biosurfactant from Pseudomons sp. G314 was a glycolipid containing carbohydrate and lipid.

Characterization of Organic Solvent Stable Lipase from Pseudomonas sp. BCNU 106 (Pseudomonas sp. BCNU 106이 생산하는 유기용매 내성 리파아제의 특성)

  • Choi, Hye Jung;Hwang, Min Jung;Kim, Dong Wan;Joo, Woo Hong
    • Journal of Life Science
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    • v.26 no.5
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    • pp.603-607
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    • 2016
  • A crude extracellular lipase from solvent-tolerant bacterium Pseudomonas sp. BCNU 106 was highly stable in the broad pH range of 4-10 and at temperature of 37℃. Crude lipase of BCNU 106 exhibited enhanced stability in 25% organic solvents such as xylene (121.85%), hexane (120.35%), octane (120.41 %), toluene (118.14%), chloroform (103.66%) and dodecane (102.94%) and showed excellent stability comparable with the commercial immobilized enzyme. In addition, the stability of BCNU 106 lipase retained above 110% of its enzyme activity in the presence of Cu2+, Hg2+, Zn2+ and Mn2+, whereas Fe2+ strongly inhibited its stability. The detergents including tween 80, triton X-100 and SDS were positive signals for lipase stability. Because of its stability in multiple organic solvents, cations and surfactants, the Pseudomonas sp. BCNU 106 lipase could be considered as a potential biocatalyst in the industrial chemical processes without using immobilization.

Characteristics of the symbionts Pseudomonas sp. J2W strain and Xanthomonas sp. J2Y strain which utilize polyvinyl alcohol (Polyvinyl alcohol 이용 공생균 Pseudomonas sp. J2W와 Xanthomonas sp. J2Y의 특성)

  • Jo, Youn-Lae
    • Applied Biological Chemistry
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    • v.35 no.1
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    • pp.30-35
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    • 1992
  • Two strains J2W and J2Y which were isolated from soil can utilize polyvinyl alcohol(PVA) as a sole carbon source. PVA was utilized symbiotically by the mixed culture of these two strains which could not utilize PVA in each respective pure culture. Effect of degree of PVA polymerization on the its utilization was examed, and there was remarkable difference among three kind of PVA(PVA 500, 1500 and 2000). The reconstruction of there two strains was carried out with other symbionts Pseudomonas sp. PW and Pseudomonas sp. G5Y which were able to utilize PVA. PVA utilization occured in each remixed culture of J2Y strain with Pseudomonas sp. PW J2W strain with Pseudomonas sp. G5Y, respectively. Identification of bacteria was based on morphological and biological chatacteristics, J2W and J2Y strain were similar to a strain of Pseudomonas pseudimallei and Xanthomonas campestris, respectively.

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Bioconversion of Pytogallo from Gallic Acid by Pseudomonas sp. KS-96 using Rotating Disc Contactor (회전원판 반응조를 이용한 Pseudomonas sp. KS-96에 의한 gallic acid로부터 Pyrogallol의 전환)

  • An, Seung-Man;Kim, Dong-Suck;Jeong, Young-Kee;Lim, Bock-Gu;Lee, Heung-Su;Ryu, Beung-Ho
    • Journal of Life Science
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    • v.7 no.2
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    • pp.112-118
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    • 1997
  • In previous paper Pseudomonas sp. Ks-96 isolated from the bioconversion into pyrogallol from gallic acid . Continuous bioconversion of pyrogallol was carried out using rotatory disc contactor immobilized Pseudomonas sp. Ks-96 . Enzyme activity of gallate decarboxylase released from Pseudomonas sp. Ks-96 were shown at the highest activity on 24h incubation. Culture media containing gallic acid supplied on the flow rate of 20m${\ell}$/h until thickness of cells wall reached steady state. Bioconversion rate of pyrogallol from gallic acid showed at highest level ranging from 18hr to 36h according to time courses. Continuous bioconversion of pyrogallol using rotating disc contactor was about 82% and 80% between 6 and 8 days at the feeding rate of 300m${\ell}$ per hour in the medium containing 15g/${\ell}$ gallic acid.

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PAHs Degrading Bacterium Separation and Identification for Biological Treatment (PAHs의 생물학적 처리를 위한 분해 미생물 분리 동정)

  • Kim, Man;Choi, Kyoung-Kyoon;Go, Myong-Jin;Park, Jeong-Hun
    • Journal of Soil and Groundwater Environment
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    • v.12 no.6
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    • pp.70-77
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    • 2007
  • Pseudomonas sp. KM1 was separated from soil contaminated by petroleum and identified. The isolated strain is Gram-positive, rod-shaped and immotile. In batch culture, the optimum cultivation temperature and pH was $35^{\circ}C$ and 7, respectively. Biodegradation of PAHs experiment with soil slurry system was performed using Pseudomonas sp. KM1. Pseudomonas sp. KM1 could degrade 7 PAHs including naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, pyrene, and fluoranthene. These mixed PAHs was easily degraded within one day except fluoranthene, which was degraded much slowly, taking several days by this isolated bacteria. Pseudomonas sp. KM1 is good candidate for bioremediation of PAHs contaminated soils. Biodegradation rates of naphthalene, phenanthrene and pyrene in soils were different at each soil, and the rates were decreased as sorption capacity increased.