• 제목/요약/키워드: Pseudomonas sp

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Isolation and Characterization of Pseudomonas sp. KM10, a Cadmium- and Mercury-resistant, and Phenol-degrading Bacterium

  • Yoon, Kyung-Pyo
    • Journal of Microbiology and Biotechnology
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    • 제8권4호
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    • pp.388-398
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    • 1998
  • A bacterium which is resistant to both mercury and cadmium, and also capable of utilizing phenol as a carbon and energy source, was isolated from the Kumho River sediments near Kangchang Bridge, Taegu, Korea. The isolate was labeled Pseudomonas sp. KM10 and characterized. The bacteria grew in 4 mM $CdCl_2$and in $70{\mu}M$ $HgCl_2$. The bacteria efficiently removed over 90% of 1 g/l phenol within 30 h. In the presence of 1.250 g/l phenol, the growth of the microorganism was slightly retarded and the microorganism could not tolerate 1.5 g/l phenol. Curing of plasmid from the bacteria was carried out to generate a plasmidless strain. Subsequent experiments localized the genes for phenol degradation in plasmid and the genes for mercury resistance and cadmium resistance on the chromosome. Dot hybridization and Southern hybridization under low stringent conditions were performed to identify the DNA homology. These results showed significant homologies between the some sequence of the chromosome of Pseudomonas sp. KM10 and merR of Shigella flexneri R 100, and between the some sequence of the chromosome of Pseudomonas sp. KM10 and cadA of Staphylococcus aureus pI258. The mechanism of cadmium resistance was efflux, similar to that of S. aureus pI258 cadA, and the mechanism of mercury resistance was volatilization, similar to that of S. flexneri R100 mer.

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Hydrolytic Dechlorination of 4-Chlorobenzoate Specified by fcbABC of Pseudomonas sp. DJ -12

  • Chae, Jong-Chan;Ahn, Kyung-Joon;Kim, Chi-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제8권6호
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    • pp.692-695
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    • 1998
  • Pseudomonas sp. DJ-12 was able to degrade 4-chlorobenzoate by hydrolytic dechlorination to produce 4-hydroxybenzoate and chloride ion. The fcbABC genes responsible for the hydrolytic dechlorination were cloned from the chromosomal DNA of the organism. The genes were found to be organized in the order fcbB-fcbA-fcbC, but there was an intergenic space between the fcbA and fcbC genes.

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Psedomonas sp.의 Catabolits Repression 저항성 변이주로부터 Cellulase의 생산 (Cellulase Production from the Catabolite Repression Resistant Mutant of Pseudomonas sp.)

  • 정영철;노종수;성낙계;강신권
    • 한국미생물·생명공학회지
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    • 제21권6호
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    • pp.549-555
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    • 1993
  • The production of cellulase by Pseudomonas sp. LBC505 isolated was under the strict genetic and biochemical control mechanisms such as catabolit repression and induction. These biochemical control reduced cellulase production. Thus LBC505 was mutated to increase enzyme yields. Cells growth and cellulase production were inhibited by the addition of 2-deoxy glucose (2-DG), which is presumed to function as repressor for the selection of high cellulase yielding mutant.

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Catabolism of 4-Hydroxybenzoic Acid by Pseudomonas sp. DJ-12

  • Tim;Chae, Jong-Chan;Kim, Chi-Kyung
    • Journal of Microbiology
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    • 제37권3호
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    • pp.123-127
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    • 1999
  • A Pseudomonas sp. strain DJ-12 isolated by 4-cholrobiphenyl enrichment culture technique is capable of utilizing 4-hydroxybenzoic acid as a sole source of carbon and energy. The bacterium catabolized 4-hydroxybenzoic acid through the intermediate formation of protocatechuic acid, which was further metabolized. The cell free extracts of pseudomonas sp. DJ-12, grown on 4-hydroxybenzoic acid showed higher activities of 4-hydroxyenzoate 3-hydroxylase and protocatechuate 4,5-dioxygenase, but the activity of catechnol 2,3-dioxygenase was lower. The results suggest that 4-hydroxybenzoic acid is catabolized via protocatechuic acid rather than catechol or gentisic acid in this bacterium and that the protocatechuic acid formed was metabolized through a metacleavage pathway by protocatechuate 4,5-dioxygenase.

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1.2-Dichlorobenzene을 분해하는 Pseudomonas sp. DCB3의 분리 및 특성 (Isolation and Characterization of Pseudomonas sp. DCB3 Degrading 1.2-Dichlorobenzene)

  • 서승교;우철주;이창호
    • 한국환경보건학회지
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    • 제23권4호
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    • pp.33-38
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    • 1997
  • Four bacterial strains able to degrade dichlorobenzene as the sole source of carbon and energy were isolated from soil by selective enrichment culture, and among them, one isolation was the best in the cell growth and identified as Pseudomonas sp. DCB3 by its morphology and physiological properties. Cell growth dramatically increased in a minimal medium containing 500ppm of dichlorobenzene was not detected any more at 72 hours after cultivation. The optimal temperature and initial pH for the growth of the isolated strain were 30$\circ$C and 7.0, respectively. Cell growth was increased by supplementing $(NH_2)_2CO$ and 50 ppm yeast extract as additional nutrients. Therefore, it was suggested that Pseudomonas sp. DCB3 could be effectively used for the biological treatment of wastewater containing dichlorobenzene.

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Isolation of pseudomonas sp. S-47 and its degradation of 4-chlorobenzoic acid

  • Seo, Dong-In;Lim, Jai-Yun;Kim, Young-Chang;Min, Kyung-Hee;Kim, Chi-Kyung
    • Journal of Microbiology
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    • 제35권3호
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    • pp.188-192
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    • 1997
  • The strain of S-47 degrading 4-chlorobenzoic acid (4CBA) was isolated from Ulsan chemical industrial complex by enrichment cultivation with 1 mM 4CBA. The strain was Gram-negative rod and grew optimally at 30.deg.C and pH 7 under aerobic condition, so that the organism was identified as a species of Pseudomonas. Pseudomonas sp. S-47 degraded 4-chlorobenzoic acid to produce a yellow-colored meta-cleavage product, which was confirmed to be 5-chloro-2-hydroxymuconic semialdehyde (5C-2HMS) by UV-visible spectrophotometry. 5C-3HMS was proved trometry. This means that Pseudomonas sp. S-47 degraded 4CBA via 4-chlorocatechol to 5C-2HMS by meta-cleavage reaction and then to 5C-2HMA by 5C-2HMS dehydrogenase.

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수종의 식물생장촉진 근권미생물의 분리 및 근권처리가 오이와 토마토 플러그묘의 초기생장에 미치는 영향 (Selection and Bacterialization into Rootzone of the Various Plant Growth Promoting Rhizobacteria in Peatmoss Compost on the Early Growth of Cucumber and Tomato Plug Seedlings)

  • 조자용;김광수;정순주
    • 한국유기농업학회지
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    • 제6권1호
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    • pp.151-160
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    • 1997
  • Azospirillum sp., photosynthetic bacteria(Rhodopseudomonas sp.) and Pseudomonas sp. were separated and screened from soil and soilless culture, and identificated. The antifungal activities against root-infected pathogens and plant growth promoting effects of the cultured solution of the starins(5.0$\times$105 cells/$m\ell$) in the peatmoss compost on the early growth of cucumber and tomato seedling were investigated. Azospirillum sp. and Pseudomonas sp. showed a antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp in thed ranges of 51.0% to 72.0% on potato dextrose agar medium, however photosynthetic bacteria had not antifungal activities. When cultured solution of Azospirillum sp., photosynthetic bacterial and Pseudomonas sp. were bacterialized by mixing with peatmoss compost, early growth of cucumber and tomato in terms of plant height, number of leaves, leaf area, root length, fresh anf dry weight of leaf, stem and root were promoted, especially photosynthetic bacteria had a the best plant growth promting activities.

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방향족화합물이 함유된 폐수의 생물학적 처리 (Microbial Degradation of Aromatic Compounds in Industrial Wastewater)

  • 박춘호;김용기;오평수
    • 한국미생물·생명공학회지
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    • 제19권6호
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    • pp.631-636
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    • 1991
  • 방향족화합물을 생분해하는 미생물을 분리하여 생물학적 처리에 응용하기 위해 폐수 및 토양에서 150종의 균을 분리하였다. 그 중에서 COD 제거율과 방향족화합물의 이용능이 가장 우수한 HC107균을 선발하여 Pseudomonas sp.로 동정하였다. 활성슬러지 장치에서 Pseudomonas sp. HC107 배양액을 2ml/day씩 처리하면서 화학, 제약 및 도료공장의 폐수를 혼합하여 연속처리한 결과 처리수의 COD, BOD 및 phenol 제거율이 평균 92.5%, 95.53 및 93%.5로 나타났다.

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Bioelectrochemical Denitrification by Pseudomonas sp. or Anaerobic Bacterial Consortium

  • Park, Doo-Hyun;Park, Yong-Keun
    • Journal of Microbiology and Biotechnology
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    • 제11권3호
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    • pp.406-411
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    • 2001
  • In a bacterial denitrification test with Pseudomonas sp. and anaerobic consortium, more nitrates and less substrate were consumed but less metabolic nitrite was produced under an anaerobic $H_2$ condition rather than under $N_2$ condition. In a bioelectrochemical denitrification test with the same organisms, the electrochemically reduced neutral red was confirmed to be a substitute electron donor and a reducing power like $H_2$. The biocatalytic activity of membrane-free bacterial extract, membrane fraction, and intact cell for bioelectrochemical denitrification was measured using cyclic voltammetry. When neutral red was used as an electron mediator, the electron transfer from electrode to electron acceptor (nitrate) via neutral red was not observed in the cyclic voltammogram with the membrane-free bacterial extract, but it was confirmed to gradually increase in proportion to the concentration of nitrate in that of the membrane fraction and the intact cell of Pseudomonas sp.

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A Pathway for 4-Chlorobenzoate Degradation by Pseudomonas sp. S-47

  • Seo, Dong-In;Chae, Jong-Chan;Kim, Ki-Pil;Kim, Young-Soo;Lee, Ki-Sung;Kim, Chi-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제8권1호
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    • pp.96-100
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    • 1998
  • Pseudomonas sp. S-47 degraded 4-chlorobenzoate (4CBA) to 4-chlorocatechol (4CC) that was subsequently ring-cleaved to form 5-chloro-2-hydroxymuconic semialdehyde. These intermediate compounds were identified by GC-mass spectrometry and UV-visible spectrophotometry. 5-chloro-2-hydroxymuconic acid converted from 5-chloro-2- hydroxymuconic semialdehyde (5C-2HMS) was dechlorinated to produce 2-hydroxypenta-2,4-dienoic acid (2HP-2,4DA) by the strain. These results indicate that Pseudomonas sp. S-47 degrades 4CBA to 2HP-2,4DA via a novel pathway including the meta-cleavage of 4CC and dechlorination of 5C-2HMS.

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