• Title, Summary, Keyword: Propolis

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Antioxidant, Antimicrobial, and Cancer Cell Proliferative Inhibition Activities of Propolis

  • Kang, Ho-Jin;Ko, Ki-Wan;Lee, Ok-Hwan;Lee, Boo-Yong
    • Food Science and Biotechnology
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    • v.18 no.4
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    • pp.1042-1045
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    • 2009
  • A commercial propolis was investigated in terms of its antioxidant, antimicrobial, and antiproliferative activities. The contents of total phenol and flavonoid of propolis were 8.3 and 6.6 mg, respectively. The reducing power of the propolis increased with concentration increasing. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was shown at 82.70% in 1,000 ${\mu}g/mL$ of the propolis. 2,2'-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging effect of antioxidant activity on the propolis was 35.64 g/sample. The propolis showed high antimicrobial activity against Bacillus cereus at all concentration of propolis. All of the cancer cell lines have 53-73% as effective growth inhibition. These results showed that the commercial propolis has potential antioxidant, antimicrobial, and cancer cell proliferative inhibition activities thus, propolis can be applied to the functional food, pharmaceutical, and cosmetic industry.

A Hydrogel Film Containing Propolis Nanoparticles as a Wound Healing Membrane

  • Kim, Jin;Kim, Yong-Moon;Kim, Dong-Woon;Lee, Ki-Young
    • Biomedical Science Letters
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    • v.18 no.2
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    • pp.175-179
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    • 2012
  • It is desirable that a wound healing membrane acts as a barrier for coverage of a damaged skin and has the biological activities such as anti-inflammatory effects. In this study, we prepared the hydrogel film containing the propolis nanoparticles as a wound healing membrane. The propolis nanoparticles were prepared by incorporation of propolis into the hydrophobic core of ${\gamma}$-cyclodextrin. The incorporation efficiency of propolis in the nanoparticles was $50{\pm}2.3%$. Propolis nanoparticles observed by a scanning electron microscope (SEM) were spherical with the size of 30~40 nm. The swelling behaviors of the hydrogel film containing propolis nanoparticles showed a similar pattern with the hydrogel film without propolis nanoparticles. The cumulative amount of propolis released from the hydrogel film containing propolis nanoparticles in the buffer of pH 7.4 and 5.5 was $86.0{\pm}2.0%$ and $64.6{\pm}1.0%$ of total propolis loaded in the hydrogel film within 9 h, respectively. These results provide a rationale for studying wound healing application of the hydrogel film containing propolis nanoparticles in a clinical setting.

Effects of propolis extracts on the immune response in cultured flounder, Paralichthys olivaceus (Propolis extracts가 양식넙치의 면역활성능에 미치는 영향)

  • Nam, Hyun Ju;Park, Kyung Il;Choi, Min Soon
    • Journal of fish pathology
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    • v.27 no.1
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    • pp.47-56
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    • 2014
  • Propolis is a beehive product with a very complex chemical composition, widely used in folk medicine because of its several therapeutic activities. This study was conducted to measure the efficacy of propolis on non-specific defense reactions, specific immune response, and protection levels against pathogen challenge with Streptococcus iniae. in vitro and in vivo. In vitro, the phagocytic activity and NBT assay of peripheral blood leukocytes (PBL) were evaluated in a various propolis extractsconcentrations (0, 10, 50, 100, 150, 250 and $500{\mu}g/ml$). The optimal concentration showing activation of propolis extracts was determined to $100{\mu}g/ml$. In vivo, they were divided into four groups (PBS, propoli extractss, vaccine, propolis extracts + vaccine) in vivo. Fish were received i.p. injection of either PBS or propolis extracts, and in the presence or absence of formalin inactivated S. iniae ($1{\times}10^8$ CFU/fish), respectively. The level of haematocrit is not affected among experimental groups. The phagocytic activity and the NBT reduction activities of head kidney phagocyte were markedly (p<0.05)augmented in the propolis extracts groups than in the PBS-control group, respectively. The level of serum lysozyme activity was significantly (p<0.05) increased in the propolis extracts treated groups than in the PBS-control group. The agglutinin titer was significantly (p<0.05) enhanced in the vaccine+propolis extracts group than in the vaccine group, but there was no difference between PBS-control and propolis treated group. The results of the present study suggest that propolis extracts seems to be a promising compounds of non-specific immune stimulator, also being able to use a good adjuvant.

Properties of Water-Soluble Propolis Made with Honey

  • Woo, Soon Ok;Han, Sangmi;Hong, Inpyo
    • Journal of Apiculture
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    • v.32 no.4
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    • pp.391-394
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    • 2017
  • Propolis is made by bees collecting protective material or essence of plants and mixing with saliva and enzymes produced by the salivary glands. It is used to repair the inside of the honeycomb, keep it sterile, and adjust the temperature and humidity. Propolis is a natural antibiotic substance that it is used to make a clean room by coating the cell before the queen bee lay eggs, and preventing the bacteria from invading by using with wax when sealing the nursery room. Propolis extract is a health functional food with antioxidant and oral antimicrobial effects. In order to use propolis in food, its active ingredients are extracted with ethanol. Water-soluble propolis was prepared by mixing and stirring honey and ethanol extracted propolis (EEP) solution. When 1kg of honey and 100ml of ethanol extracted propolis solution were mixed and stirred, the total flavonoid content of water-soluble propolis was $6.6{\pm}1.1mg/10g$, and the free radical scavenging effects of water-soluble propolis were 54 to 74%.

In vitro Evaluation of the Antifungal Activity of Propolis Extract on Cryptococcus neoformans and Candida albicans

  • Chee, Hee-Youn
    • Mycobiology
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    • v.30 no.2
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    • pp.93-95
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    • 2002
  • The antifungal activities of propolis on Cryptococcus neoformans and Candida albicans were evaluated. In microbroth culture assay, the MIC(minimum inhibitory concentration) of propolis for C. neoformans and C. albicans were 2 and 16 mg/ml, respectively. In propolis-included solid medium assay, the MIC of propolis for C. neoformans and C. albicans were 4 and 16 mg/ml, respectively. Propolis showed fungicidal activity against C. neoformans, whereas propolis possesed fungistatic activity against C. albicans. The MFC(minimum fungicidal concentration) for C. neoformans was 8 mg/ml. Cell morphology of C. neoformans was affected by treatment of propolis. In scanning electron microscope, the appearance of cell rupture was observed.

Propolis Inhibits UVA-Induced Apoptosis of Human Keratinocyte HaCaT Cells by Scavenging ROS

  • Kim, Han Bit;Yoo, Byung Sun
    • Toxicological Research
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    • v.32 no.4
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    • pp.345-351
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    • 2016
  • Propolis is a resinous material collected by honeybees from several plant sources. This research aimed at showing its protective effect against UVA-induced apoptosis of human keratinocyte HaCaT cells. Using Hoechst staining, it was demonstrated that propolis (5 and $10{\mu}g/mL$) significantly inhibited the apoptosis of HaCaT cells induced by UVA-irradiation. Propolis also showed the protective effect against loss of mitochondrial membrane potential induced by UVA-irradiaiton in HaCaT cells. Propolis also inhibited the expression of activated caspase-3 induced by UVA-irradiation. To investigate the role of ROS in UVA-induced apoptosis and protection by propolis, the generation of ROS was determined in cells. The results showed that the generation of ROS was markedly reduced in cells pretreated with propolis. Consequently, propolis protected human keratinocyte HaCaT cells against UVA-induced apoptosis, which might be related to the reduction of ROS generation by UVA-irradiation.

Antibacterial Effects of Propolis Extracts on Pathogenic Bacteria (Propolis 추출물의 병원성 미생물에 대한 항균 효과)

  • Cho Jung-Soon;Kim Young-Hwu;Kwon Myong-Sang
    • Journal of the East Asian Society of Dietary Life
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    • v.15 no.4
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    • pp.457-464
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    • 2005
  • The optimal concentration of ethanol to separate a high quantity of propolis was $60\%$ but that for the best flavonoids extraction was $80\%$ We compared the yields of propolis from different countries. In this study we used $60\%$ ethanol concentration as a standard. The yield of propolis was proportional to the contents of flavonoids. Namely, Polish propolis which showed the highest yield with $56\%$ by the extraction with $60\%$ ethanol revealed also the highest flavonoids content with $3.49\%$ among all the samples tested The major constituents of propolis differed from country to country. It has been suggested that the different geographical origin influenced the efficacy and the constituents of propolis. Antibacterial activity of ethanol extracted propolis from different countries was tested against 6 microbial strains of type cultures including Gram-positive (Staphylococcus aureus, Streptococcus uberis, Streptococcus agalactiae) and Gram- negative bacteria (Klebsiella pneumoniae, Proteus vulgaris and E coli) in vitro. Propolis extract showed anti-microbial activity against all the tested bacterial strains. In addition, propolis was sensitive to E coli which was resistant to broad spectrum antibiotics like ampicillin. These results showed that propolis may substituted for commercial antibiotics. The efficiency of anti-microbial activity of the propolis was slightly higher in $80\%$ than $97\%$ ethanol extract.

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Effects of Propolis Treatment on the Quality of Dried Squid (Propolis 의 처리가 마른 오징어의 품질에 미치는 영향)

  • Yang, Seung-Yong;Lee, Nam-Hyouck;Hong, Sang-Pill;Bang, Hyun-A
    • Korean Journal of Food Science and Technology
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    • v.31 no.2
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    • pp.356-360
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    • 1999
  • Effect of propolis treatment on the quality of dried squid were investigated. Dried squid containing 29% of moisture content were prepared and treated with 0.25% and 0.5% of propolis. Its total microbial count, TBA value and browning degree were assayed during 30 days. Total microbial count of dried squid treated with propolis were $72{\sim}80\;cells/g$, compared with control showing 25,000 cells/g. TBA values of dried squid were by $0.1{\sim}0.2$ lower than control. Browning degree was not different between treatment and control, but browning degree of propolis treatment showed lower than control through colorimetric analysis. These results suggested that quality of dried squid containing higher moisture could be improved by propolis treatment.

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Studies on the Flavonoid Compositions of Korean Propolis (국산 프로폴리스의 플라보노이드 조성에 관한 연구)

  • 이수원;김희재;양희진;황보식
    • Food Science of Animal Resources
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    • v.21 no.4
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    • pp.389-394
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    • 2001
  • This study was conducted to investigate the flavonoid contents of Korean propolis. The total flavonoids content measured by spectrophotometer of Yecheon, Youngwol, Brazilian, Chinese. Aus- tralian propolis were 6.33, 6.43, 2.44, 6.52 and 8.11mg/g. The p-coumaric acid content of Yecheon and Youngwol propolis were 5.58 and 6.84mg/100g, respectively. Luteolin, however, was not detected both in Yecheon and Youngwol, respectively. the quercetin content of Yecheon and Youngwol propolis revealed between 0.41 to 0.53%, however, overseas propolis was not detected. The t-cinnamic acid of Brazilian propolis was 7.92% and Chinese propolis was 8.74%. And than, the t-cinnamic acid of Chinese propolis was not detected.

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Studies on the Efficacies of Water Extract of Propolis (프로폴리스 물추출물의 약효연구)

  • 최혁재;심상범;김남재;김종우
    • Biomolecules & Therapeutics
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    • v.6 no.3
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    • pp.261-268
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    • 1998
  • Propolis is a lipophilic, natural product prepared by mixing the exudates collected from various plants by honeybees with beeswax for the purpose of using to seal hive walls and to strengthen the borders of combs. Because of its versatile bioactivities, propolis has been attracting many investigators'interest. But the pharmacological studies on propolis has, to date, been exclusively performed for an alcohol extract, there is few information of water extract. Therefore, in this study, we investigated the various effects of water extract of Chinese propolis. The water extract of propolis and its fractions of organic solvents showed strong antioxidative activities, especially ether and ethylacetate fractions, and reduced the lipid peroxidation of rat liver in viro. Additionally the ether fraction of propolis (10 mg/ml) inhibited the activity of hyaluronidase by 50%. In vivo, the water extract of propolis considerably decreased s-GOT, s-GPT and s-LDH activities which represent for the hepatotoxicity induced by $CCl_4$ in rats, and prolonged the MST (Medium revival tinge) and ILS (Increasing in MST over control) by 18% in mice which inoculated with sarcoma 180 ascites cells. These results suggest that the water extract of propolis has various bioactivities as well as the alcohol extract.

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