• Title, Summary, Keyword: Plutella xylostella

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Antifeeding Activity and Chitin Synthesis Inhibition Activity of the Ultrasonic Treatment on Plant Extract against Plutella xylostella(Lepldoptera : Yponomeutidae) (초음파 처리 식물체의 배추좀나방 유충에 대한 섭식 저해활성 및 카틴합성 저해활성)

  • 방혜선;이완주;황석조;구태원;김선여
    • Journal of Life Science
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    • v.9 no.3
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    • pp.248-252
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    • 1999
  • Effects of the ultrasonic treatment on plant represents antifeeding activity and chitin synthesis inhibition activity against diamondback moth (Plutella xylostella L.) by a leaf-dipping method at a concentration of 5,000ppm. Methanol extracts of leaves of the ultrasonic treatment on plant was sequentially partitioned with n-hexane, chloroform, ethlyacetate, butanol and water fractions, and bioassayed. Antifeeding activities were observed in n-hexane, chloroform and ethlyacetate fractions. N-hexane fraction has 94.6% of strongest antifeeding activity against P. xylostella. From the n-hexane sequentially partitioned showed H6 and H8 fractions of antifeeding activities against P. xylostella. And chitin synthesis inhibition activity against P. xylostella was obtained the methaol, n-hexane and water fractions.

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Enhanced Insecticidal Activity of Bacillus thuringiensis against the Diamondback Moth, Plutella xylostella, Using an Immunosuppressive Effect of Juvenile Hormone Analogue, Pyriproxyfen, Formulation (유약호르몬 유사체인 피리프록시펜 제제의 배추좀나방(Plutella xylostella) 면역 억제 효과와 이를 이용한 Bacillus thuringiensis 살충력 제고 기술)

  • Kim, Geun-Seob;Kim, Yong-Gyun
    • The Korean Journal of Pesticide Science
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    • v.13 no.2
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    • pp.111-116
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    • 2009
  • Juvenile hormone (JH) is an insect hormone mediating immature metamorphosis and adult reproduction. It also mediates immune responses to suppress hemocyte behavior, which is, however, activated by ecdysteroid. This study investigated an effect of a commercial pyriproxyfen (a JH agonist) formulation on a cellular immune response of the diamondback moth, Plutella xylostella, and analysed its mixture with Bacillus thuringiensis (Bt) in insecticidal potency. The commercial pyriproxyfen formulation significantly suppressed hemocyte-spreading behavior at low doses as did in pyriproxyfen technical grade. When the commercial pyriproxyfen formulation was mixed with Bt, Bt toxicity was significantly increased against P. xylostella larvae in laboratory. The mixture effect was then confirmed in field cultivating cabbage infested with P. xylostella larvae. The mixture showed a significantly enhanced mortality and reduced effective lethal time, compared to only Bt treatment.

Genetic Analysis of the Diamondback Moth, Plutella xylostella, Collected from China Using Mitochondrial COI Gene Sequence

  • Li, Jianhong;Choi, Yong Soo;Kim, Iksoo;Sohn, Hung Dae;Jin, Byung Rae
    • International Journal of Industrial Entomology
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    • v.9 no.1
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    • pp.137-144
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    • 2004
  • The diamondback moth, Plutella xylostella, is notorious because of its extensive potential and actual dispersal ability. Previously, the Korean populations of P. xylostella was extensively collected and analyzed for their genetic population structure using a portion of mitochondrial DNA (mtDNA). One of the postulated characteristics on population genetic structure of the species includes the presence of heterogeneous haplotypes, possibly possessed by some dispersed ones from neighboring countries. In this study, we sequenced ten P. xylostella collected from China (∼2,000 km away from the middle part of Korea) to know the genetic relationships of these to the Korean P. xylostella. Sequence analysis of the identical portion of COI gene resulted in five haplotypes with the sequence divergence ranging from 0.5% (two nucleotides) to 1.1 % (five nucleotides) among them and from 0.7% (three) to 2.5% (11) to the pre-existing 52 Korean haplotypes. Phylogenetic analysis showed that the Chinese P. xylostella were neither clearly separated from the Korean haplotypes nor clustered with one heterogeneous Korean haplotype. This result reinforces the significance of gene flow in this species and suggests to exclude the possibility that the heterogeneous Korean haplotypes may have emigrated from China, where our samples were obtained, although further extensive investigation is required.

Attractive Effects Efficiency of LED Trap on Controlling Plutella xylostella Adults in Greenhouse (LED 트랩을 이용한 온실내 배추좀나방에 대한 유인효과)

  • Park, Jun-Hwan;Lee, Sang-Min;Lee, Sang-Guei;Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.57 no.3
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    • pp.255-257
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    • 2014
  • This study was conducted to determine the attractive effects of Plutella xylostella adults to light emitting diode (LED) trap in greenhouse and compared with those of no light trap and black-light which is typically used in commercial luring lamp. The green LED trap captured more P. xylostella when compared with black-light trap, whereas the no light trap was a little attractive to P. xylostella adults. These results indicated that the green LED traps could be used for environmental insect pest control.

An Integrated Biological Control Using an Endoparasitoid Wasp (Cotesia plutellae) and a Microbial Insecticide (Bacillus thuringiensis) against the Diamondback Moth, Plutella xylostella (배추좀나방에 대한 프루텔고치벌과 미생물농약의 통합생물방제)

  • Kim, Kyusoon;Kim, Hyun;Park, Young-Uk;Kim, Gil-Hah;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.52 no.1
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    • pp.35-43
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    • 2013
  • All tested Korean populations of the diamondback moth, Plutella xylostella, are known to be resistant especially against pyrethroid insecticides by mutation in its molecular target, para-sodium channel. Moreover, P. xylostella is able to develop resistance against most commercial insecticides. This study was performed to develop an efficient control technique against P. xylostella by a combined treatment of an endoparasitoid wasp, Cotesia plutellae, and a microbial insecticide, Bacillus thuringiensis. To investigate any parasitism preference of C. plutellae against susceptible and resistant P. xylostella, five different populations of P. xylostella were compared in insecticide susceptibilities and parasitism by C. plutellae. These five P. xylostella populations showed a significant variation against three commercial insecticides including pyrethroid, organophosphate, neonicotinoid, and insect growth regulator. However, there were no significant differences among five P. xylostella populations in their parasitic rates by C. plutellae. Moreover, parasitized larvae of P. xylostella showed significantly higher susceptibility to B. thuringiensis. As an immunosuppressive agent, viral ankyrin genes (vankyrins) encoded in C. plutellae were transiently expressed in nonparasitized larvae. Expression of vankyrins significantly enhanced the efficacy of B. thuringiensis against the third instar larvae of P. xylostella. Thus an immunosuppression induced by C. plutellae enhanced the insecticidal efficacy of B. thuringiensis. These results suggest that a combined treatment of C. plutellae and B. thuringiensis may effectively control the insecticide-resistant populations of P. xylostella.

Determination of the effective spray- interval of Bacillus thuringiensis against diamond-back moth (Plutella xylostella) on chinese cabbage

  • Han, E.J.;Park, J.H.;Hong, S.J.;Ahn, N.H.;Jee, H.J.;Kim, Y.K.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.259-262
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    • 2011
  • In organic Chinese cabbage fields, Commercial Bacillus thuringiensis products are used widely against diamond back moth, Plutella xylostella. We conducted the study to determine the effective spray-interval of commercialized B. thuringiensis against diamond back moth on Chinese cabbages. Chinese cabbage leaves were collected 0, 1, 2, 3, 6, 10days after treatment in first trial and 0, 2, 4, 7, 9, 11days after treatment. We compared the insecticidal property of sprayed B. thuringiensis and the density of it on surface of Chinese cabbages using collected leaves. The insecticidal property maintained high until nine days after commercial B. thuringiensis products sprayed.

Pathogenicities of Entomopathogenic Fungus, Metarhizium anisopliae J-22 against Turfgrass and Some Agro-forest Insect Pests (골프장 잔디 및 농림해충 수종에 대한 곤충병원성 곰팡이 Metarhizium anisopliae J-22의 병원성)

  • 이상명;이동운;추호렬;문일성;이태우
    • Asian Journal of Turfgrass Science
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    • v.11 no.3
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    • pp.185-191
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    • 1997
  • Biological control of turfgrass insect pest Blitopertha orientalis, forest insect pests, Agelastica coerulea, Meganola melancholia, and Glyphodes perspectalis,vegetable insect pests, Plutella xylostella and Agrotis segetum were conducted with entonopathogenic fungus, Metarhizium anisopliae J-22 isolated from black pine forest soil in Cheju province. Mortality of B. orientalis larvae was 53.3% at the rate of 3.4 $\times$ 1O 7 conidia /ml. A. coerulea and M melancholia larvae showed 100% mortalities at 9.6 $\times$ 106 conidia /ml and 2.7 $\times$ 10 7 conidia /ml as well. However, G. perspectalis larvae were not dead even at 4$\times$ 1O 7 conidia /ml. On the other hand, M anisopliae J-22 was effective against P. xylostella larvae showing 100% mortality at 4 $\times$ 10 7 conidia /ml. KEy words:Entomopathogenic fungi, Biological control, Metarhizium anisopliae, Pathogenicity,Blitopertha orientalis, Agelastica coerulea, Meganola melancholia, Glyphodes perspectalis,Plutella xylostella, Agrotis segetum.

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Test of Larvicidal Effect of Some Commercial Natural Products on Lepidoptran Plutella xylostella and Spodoptera litura Larvae

  • Jeong, Hyung-Uk;Im, Hyun-Hwak;Chang, Sung-Kwon;Paik, Chae-Hoon;Han, Tae-Ho;Kim, In-Seon;Kim, Ik-Soo
    • International Journal of Industrial Entomology
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    • v.15 no.1
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    • pp.87-91
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    • 2007
  • A diverse kind of environment-friendly agricultural materials(EFAM) for the control of insect pests is on the market. These EFAMs are a part of essential sources for the accomplishment of successful, sustainable, and environment-friendly agriculture. Thus, accurate information of these EFAMs is one that required for the success of environment-friendly agriculture, but, in reality, still appropriate information is absolutely in shortage. In this study, we, therefore, tested the efficacy of commercial EFAMs against two lepidopteran insect larvae, the diamondback moth Plutella xylostella(Lepidoptera: plutellidae) and the tobacco cutworm Spodoptera litura(Lepidoptera: Noctuidae). After the two insect pests were successfully stabilized in indoor environment the larvicidal activity was tested at $24{\pm}1^{\circ}C$, relative humidity(RH) of $60{\pm}5%$, and a photoperiod of 16L:8D, and mortality was determined 48 hrs after EFAMs are treated. The EFAMs that showed more than 90% of larvicidal activity were each six among 16 against both P. xylostella and S. litura and only three of them showed consistent larvicidal activity against both species, signifying species specificity of EFAMs and importance of selection of proper EFAMs depending on target insect pest.