• Title, Summary, Keyword: Phytase

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Purification of Phytase from Aspergillus ficuum and Production of Anti-phytase Antibody (Aspergillus ficuum의 Phytase의 정제와 Anti-phytase 항체생산)

  • Kim, Keun
    • The Korean Journal of Mycology
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    • v.27 no.4
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    • pp.299-303
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    • 1999
  • Phytase(myo-inositol-hexakis phosphate 3-phosphohydrolase, E C 3.1.3.8) sequentially hydrolyzes phytate to myo-inositol and inorganic phosphate. Phytase of Aspergillus ficuum was purified to homogeneity using ultrafiltration, cation exchange column and anion exchange column. It's molecular weight is estimated as around 90,000 by SDS-PAGE. Antibody against the phytase was produced by immunizing mice with the purified phytase. The titer of the antibody was determined to be 1/25,000.

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Effect of Supplemental Microbial Phytase and Nonphytate Phosphorus Levels on Performance, Digestibility and Egg Quaility in Layer (Microbial Phytase와 무기태 인 수준별 급여가 산란 생산성, 소화율 및 계란 품질에 미치는 영향)

  • 김상호;유동조;박수영;이상진;박용윤;이원준
    • Korean Journal of Poultry Science
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    • v.27 no.3
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    • pp.243-254
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    • 2000
  • The effect of supplemental microbial phytase and non - phytate phosphorus(NPP) levels on layer productivity and nutrient digestibility were conducted in 640 21 weeks - old HyLine brown layer for 12 weeks. Supplemented phytase levels were 0, 300, 500 and 1,000 DPU/kg diet. NPP levels were adjusted with tricalcium phosphate(TCP), which were 0(0.11% NPP), 0.5(0.20), 1.0(0.29) and 1.5%(0.38). ME, CP and Ca levels were maintained at 2,800㎉/kg diet, 16% and 3.5%, respectively. Egg production was increased with phytase compared to without phytase(P〈0.05). Increasement of egg production was higher latter of experimental period. Egg production was not different to phytase levels. Egg production in TCP levels were increased in above 0.5% compared to 0% TCP. Difference of egg production by TCP was higher after 6 week. Especially, egg production to supplemental phytase was higher in 0% TCP. Egg weight was not different to phytase and TCP levels. Egg mass was increased with phytase compared to without phytase, but not difference significantly. There was similar to phytase levels. Egg mass in TCP group was increased in TCP supplementation(P〈0.05). Feed intake was not different in phytase levels, and greater with increasing TCP levels(P〈0.05). Feed conversion was improved with phytase(P〈0.05), and not difference in TCP levels. All of nutrients digestibility tended to improve with phytase, P(P〈0.05), especially. There were not different among phytase levels. The effect of adding phytase was higher in low phosphorus diets compared normal levels. Eggshell breaking strength and eggshell thickness also improved in added phytase(P〈0.05). Tibial ash and P content were slightly increased with phytase, and Ca content also was higher(P〈0.05) compared without phytase. We concluded that supplemental phytase in low phosphorus diet was showed to increase laying performance, feed efficiency, nutrients digestibility, egg quality, and bone development. Phytase supplementation was able to compensate for low NPP diet. We also thought optimum phytase level is 300 DPU, and can decrease NPP supplementation adding phytase in later diet.

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Effect of Dietary Phytase on Growth Performance and Excreta Excretion of Growing-Finishing Pigs (사료내 Phytase 첨가가 육성비육돈의 생산성과 분뇨 배설량에 미치는 영향)

  • HwangBo, Jong;Hong, Eui-Chul
    • Journal of Animal Environmental Science
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    • v.17 no.sup
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    • pp.35-42
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    • 2011
  • This work was conducted to investigate the effect of dietary phytase on growth performance and excreta excretion of growing-finishing pigs for 7 days. Eighty three crossbreeds (Yorkshire ${\times}$ Landrace ${\times}$ Duroc) of growing-finishing pigs were used in this work, and divided into 6 treatments. Six treatments were compared in a $2{\times}2$ factorial arrangement with 2 groups (5 replications/group, 8 head/replications) with the additive phytase and 3 groups(growing I, II and finishing phase) with growing phases. Initial weights with growing phases were $58.6{\pm}3.9$. $83.2{\pm}3.8$ and $111.4{\pm}5.4kg$, respectively. Body weight gain was high in phytase treatment (P<0.01) and low at finishing phase. Feed conversion ratio was high in no phytase treatment and at finishing phase (P<0.05). Feed and water intakes have no significant difference with phytase existence and growing phases. Feces excretion decreased with growing phase (P<0.05), and was low at phytase treatment (P<0.05). There was no significant difference on urine excretion (P>0.05). Nitrogen (N) and phosphorus (P) intake was not found significant difference with phytase and growing phases. N excretion had no significant difference on phytase existence and growing phase (P>0.05), but P excretion decreased at phytase treatment (P<0.05). N and P excretion ratio was low at phytase treatment (P<0.05). Finally, dietary phytase resulted in improvement of growth performance and reduction of excreta excretion of growing-finishing pigs.

Effect of Crude Phytase Supplementation on Performance of Broilers Fed Different Levels of Phosphorus (인 수준이 다른 육계사료에 Crude Phytase 첨가시 생산성에 미치는 영향)

  • 이선재;엄재상;백인기;이재관
    • Korean Journal of Poultry Science
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    • v.27 no.3
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    • pp.169-179
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    • 2000
  • An experiment was conducted to measure the effect of crude phytase supplementation on the growing performance, blood concentrations of some minerals and tibia characteristics of broiler chickens. Day-old 240 male broiler chickens (Avian) were randomly allotted to four treatments. There were six replicates per treatment, and ten chicks per replicate. Treatments consisted of two levels of crude phytase (0 and 600U/kg) made from Aspergillus ficuum and two levels of non-phytate P (0.45 and 0.35% NPP for the starter period, and 0.35 and 0.25% NPP for the grower period), making the experiment 2$\times$2 factorial. The starter period was from hatch to 21 d of age, and grower period was from 22 to 35 d of age. Feed intake and weight gain of chicks fed diet containing phytase were higher(P〈0.05) than those of chicks fed diets without phytase, however, no differences was found in feed/gain. mortality, and nutrient availabilities regarding the phytase supplementation. Chickens fed diets with low NPP and phytase excreted lower P than did birds fed diets containing normal NPP without phytase. The level of NPP and phytase did not affect N excretion. The Ca availability was increased by feeding low NPP diet. Dietary phytase increased the availabilities of P and Mg, but decreased those of Fe and Zn. There was interactions between dietary NPP level and phytase addition on mineral availability. Tibia was lighter and shorter in low NPP groups, and heavier in phytase treated groups. The tibial contents of Ca, P and Mg decreased in low NPP treated groups, but increased in phytase treated groups. The ash content of tibia of chickens fed diet with phytase was higher than that of birds fed diets without phytase. These data suggest that the crude phytase supplementation to broiler diets containing low NPP level improves growth performance and mineral availability and, reduces fecal P excretion.

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Distribution of Phyrase in The Development Rat Organs (성장 발육에 따른 흰쥐 장기내 phytase의 분포성)

  • 양원진
    • Journal of Life Science
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    • v.7 no.2
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    • pp.127-133
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    • 1997
  • The phytase(myo-inositol hexkisphosphate phosphohydrolase ; EC 3.1.3.8) activity was observed only in the homogenate of intestinal mucosa, though the activity of alkaline phisphatase was measurable in various organs. In addition, no protein bands were detected in any other organs on immunoblotting using the anti-90kDa phytase antiserum. Thses results suggest that phytase is specifically present in small intestinal mucosa, and that hydrolysis of phytic acid(inositol-hexakisphosphate) can be allotted for a physiological role of the intestine-specific enzyme. The activities of phytase was increased during development of rat. The 70kDa phytase appeared just after birth, but the 90kDa phytase was not observed until adult period, suggesting that the 90kDa phytase was synthesized in response to weanling.

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Isolation of Phytase Producing Pseudomonas fragi and Optimization of its Phytase Production (Acid Phytase를 생산하는 Pseudomonas fragi의 분리와 phytase의 생산조건)

  • Kim, Young-Jin;Jang, Eun-Seok;In, Man-Jin;Oh, Nam-Soon
    • Applied Biological Chemistry
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    • v.46 no.4
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    • pp.291-298
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    • 2003
  • A bacterial strain producing a high level of an extracellular phytase was isolated from livestock waste water, identified as a strain of Pseudomonas fragi and designated as Pseudomonas fragi Y9451. Under the phytase production medium, the activity of phytase reached the highest level after 120 hours of incubation. On the effect of carbon sources on the phytase production, the most favorable carbon source for phytase production was fructose. As for the effect of nitrogen sources, high levels of phytase activity were detected in the medium containing nutrient broth as the nitrogen source. Free $PO_4^{3-}$ inhibited phytase production with increasing concentration of $KE_2PO_4$ and phytate in the media. The addition of $CaCl_2$ and $MgSO_4$ also resulted in the inhibition of phytase production. To investigate the effect of aeration on the phytase production, different volumes of culture broth in Erlenmeyer flasks were incubated in rotary shaker at the speed of 200 rpm. As a result, a high level of phytase activity was detected at small volume of culture broth as compared to larger volume because of its more aerobic condition.

Production and Reaction Properties of Phytase by Saccharomyces cerevisiae CY strain (Saccharomyces cerevisiae CY 균주에 의한 Phytase의 생성과 반응특성)

  • Seo, Sung-Won;In, Man-Jin;Oh, Nam-Soon
    • Applied Biological Chemistry
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    • v.48 no.3
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    • pp.228-232
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    • 2005
  • A yeast strain producing phytase, isolated from a mash of Korean traditional Yakju, was identified as a strain of Saccharomyces cerevisiae and designated as Saccharomyces cerevisiae CY strain. Phytase was produced by CY strain both intracellularly and extracellularly. Total phytase activity by the shaking culture was about two times higher than that of the static culture. The portion of extracellular phytase to total phytase activity ranged between 23 and 49 percent, depending on the glucose concentration in the culture medium. Phytase production was reached at approximately 1 U/ml as total phytase activity and the maximum intracellular phytase activity was 0.17-0.19 U/mg-DCW at late logarithmic growth phase. The optimum reaction pH and temperature of intracellular phytase were 3.5 and $40^{\circ}C$, respectively. Over 95% of the phytate was degraded by growing cells after 36 hours yeast cell culture and about 90% of total phytate was effectively degraded by suspending the whole cell with the biomass of 0.4 mg-DCW/ml-reaction solution after 12 hours degradation reaction.

Effect of Dietary Phytase on Growth Performance and Excreta Excretion of Broilers (사료내 Phytase 첨가가 육계의 생산성과 분뇨 배설량에 미치는 영향)

  • HwangBo, Jong;Hong, Eui-Chul;Kang, Bo-Seok;Kim, Hak-Kyu;Heo, Kang-Nyeong;Choo, Hyo-Jun;Kim, Won
    • Korean Journal of Poultry Science
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    • v.38 no.4
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    • pp.255-263
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    • 2011
  • This work was conducted to investigate the effects of phytase on the growth performance, excreta excretion, and N and P excretion of broilers for 7 weeks. One hundred ninety two Ross broiler (one-day-old) with average weight $44.7{\pm}1.5$ g were assigned randomly to 4 treatments groups (12 birds/pen, 4 pens/treatment) as $2{\times}2$ complex factors of sex of broiler male, female) and phytase 2 treatments (0, 300 FTU/kg), and fed experimental diets for 6 weeks (starter, 0~2 wk; earlier, 2~4 wk; finisher, 4~7 wk). The experimental diets included that phytase was added to the basal diet. Body weight was high at male treatment and at phytase treatment. Feed intake was high at male treatment and was no difference between phytase and non-phytase treatment. Feed efficiency was the highest at male and phytase treatment. Manure excretion was high at male treatment and reduced at phytase treatment. Excretion of N and P was high at male treatment and at non-phytase treatment. Finally, when phyase was added to basal diet, the growth performance of broilers was improved and excreta excretion and N and P excretion of broilers were decreased.

The Effect of Dietary Phytase on Nitrogen and Phosphorus Excretion of Gestating and Lactating Sows (Phytase 첨가가 임신돈과 포유돈의 분뇨 배설량 및 질소와 인 배설량에 미치는 영향)

  • HwangBo, Jong;Hong, Eui-Chul
    • Journal of Animal Science and Technology
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    • v.54 no.4
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    • pp.291-297
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    • 2012
  • This study was carried out to investigate the effect of dietary phytase on nitrogen and phosphorus excretion of gestating and lactating sows (Yorkshire ${\times}$ Landrace ${\times}$ Duroc). Twelve gestating sows and twelve lactating sows were used in this study and were divided into 2 groups (1 control group and 1 treatment group, 6 replications/group) the control group was without phytase and the treatment group was fed with phytase (750 FTU/kg) in the diet, respectively. Body weights of gestating and lactating sows were $208.9{\pm}13.8$ kg and $190.5{\pm}22.9$ kg, respectively. In gestating sows, feed intake was greater in phytase fed group than the control (P<0.05), but water intake and total excretion were not different between the groups. In lactating sows, feed and water intakes and total excretion were not different between the groups. The N intake of lactating sows was higher in phytase fed group than control (P<0.05) but N excretion ratio was not different. In lactating sows, N intake and excretion and N excretion ratio were not significantly different between the groups. P excretion and excretion ratio in gestating sows decreased by phytase treatment (P<0.05) as compared to control. In lactating sows, N intake and excretion was not significantly different by added phytase (P>0.05). Finally, dietary addition of phytase decreased P excretion in feces of gestating and lactating sows.

Progressive Screening of Thermostable Yeasts for Phytase Production

  • Ries, Edi Franciele;Macedo, Gabriela Alves
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.655-660
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    • 2009
  • Biotechnological phytase preparations are commercially available and are currently used in animal feeding. However, thermostability constraints, low yields, and the high cost of the enzyme have limited its use. This study represents a new perspective for the food enzyme market. The research screened thermostable yeast strains for their ability to produce phytase. The screening was carried out with a gradual increase in temperature ($30-48^{\circ}C$). Sixteen strains (1 strain identified as Saccharomyces cerevisiae) maintained the ability to produce phytase at $48^{\circ}C$ and their phytase activity was confirmed using 2 phytase assay methodologies. The yeast strains tested in this study seem to be potential efficient producers of phytase, indicating a possible new source of thermostable phytase of commercial interest, particularly that from S. cerevisiae.