• Title, Summary, Keyword: Passive Immunization

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Active and Passive Protective Effect of CFC-101 (Pseudomonas Vaccine) in Mice (CFC-101(녹농균 백신)의 능동 및 수동면역 효과)

  • 박완제;조양제;김영지;김제학;박관하;김유삼;함경수
    • Biomolecules & Therapeutics
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    • v.2 no.4
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    • pp.326-330
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    • 1994
  • The treatment of pseudomonal infection is a perplexed problem because of its modest susceptibility to most of the major antibiotics. A novel Pseudomonas vaccine(CFC-101) was prepared from the outer membrane protein fractions of several Pseudomonas strains. In this study, we examined CFC-101's effectiveness in both active and passive immunization models. CFC-101 in mice at 0.2 mg/kg, i.p., given three times at two-day intervals, completely prevented the death caused by Pseudomonas aeruginosa. Antibody titer, in accordance with the protective effect in this active immunization, was elevated to its peak level following three consecutive administrations of CFC-101. Thereafter, antibody titer stayed at a constantly high level. Each outer membrane protein fraction from the four CFC-101 producers, exhibited good cross-protective effects in mouse infection models against different Fisher types of P. aeruginosa. In the passive immunization model, 21~336 $\mu\textrm{g}$/kg of anti-rabbit IgG to CFC-101, when mice being infected with a challenge strain, prevented the Pseudomonhas-induced death up to 60%. Therefore, the preventive effect of CFC-101 was verified in both the active and passive immunization models.

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The Effects of Somatotropin Passive Immunization on Milk Yield, Plasma Hormone and Metabolite Concentrations in Rats

  • Sun, S.S.;Jang, K.;Kuk, K.;Myung, K.H.;Choi, Y.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.3
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    • pp.324-328
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    • 1997
  • The objectives of this study were to measure growth rate and endocrine changes and to improve milk production by somatostatin passive immunization in rat. Experimental animals were 10 weeks old 20 Sprague-Dawley rats. The rats were randomly assigned each 10 in control (normal sheep serum injected: NSS) and treatment (anti-somatostatin injected), and pre-fed for 2 weeks. Anti-somatostatin was purified from serum of 1 year old sheep after somatostatin active immunization, and was injected daily to rats, and growth rate and milk yield were measured for 14 days. Growth rate of litters was 2.15 g/d and 2.32 g/d in NSS and anti-somatostatin injected, respectively. Milk production was increased 6.2% in day 8 and 6.5% in day 12 by anti-somatostatin injection. Plasma growth hormone, insulin, glucose, and urea-N were increased, but non-esterified fatty acid was decreased by anti-somatostatin injection. In summary, passive immunization of somatostatin improved growth rate of litters and milk production in rats.

Recent Advances in Toxoplasma gondii Immunotherapeutics

  • Lim, Sherene Swee-Yin;Othman, Rofina Yasmin
    • The Korean Journal of Parasitology
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    • v.52 no.6
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    • pp.581-593
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    • 2014
  • Toxoplasmosis is an opportunistic infection caused by the protozoan parasite Toxoplasma gondii. T. gondii is widespread globally and causes severe diseases in individuals with impaired immune defences as well as congenitally infected infants. The high prevalence rate in some parts of the world such as South America and Africa, coupled with the current drug treatments that trigger hypersensitivity reactions, makes the development of immunotherapeutics intervention a highly important research priority. Immunotherapeutics strategies could either be a vaccine which would confer a pre-emptive immunity to infection, or passive immunization in cases of disease recrudescence or recurrent clinical diseases. As the severity of clinical manifestations is often greater in developing nations, the development of well-tolerated and safe immunotherapeutics becomes not only a scientific pursuit, but a humanitarian enterprise. In the last few years, much progress has been made in vaccine research with new antigens, novel adjuvants, and innovative vaccine delivery such as nanoparticles and antigen encapsulations. A literature search over the past 5 years showed that most experimental studies were focused on DNA vaccination at 52%, followed by protein vaccination which formed 36% of the studies, live attenuated vaccinations at 9%, and heterologous vaccination at 3%; while there were few on passive immunization. Recent progress in studies on vaccination, passive immunization, as well as insights gained from these immunotherapeutics is highlighted in this review.

Effects of Active and Passive Distraction Intervention on Pain Responses of Preschool Children during Immunization (능동적 및 수동적 관심전환 중재가 예방접종 시 학령전기 아동의 통증반응에 미치는 효과)

  • Im, Eun-Seon;Kim, Jin-Sun
    • The Journal of the Korea Contents Association
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    • v.16 no.12
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    • pp.102-112
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    • 2016
  • The purposes of this study were to identify the effects of distraction strategies compared to usual pain management, and to compare the effects of passive and active distraction on pain responses of preschool children during immunization. A quasi experimental with non-equivalent control group posttest design was used. Participants for each group were 30 preschool children who visited a pediatric clinic to have influenza immunization. Children in experimental groups selected one of two distraction types. Pain responses were measured by children, parents, and researcher. Pain responses by children, parents, and researcher during immunization were significantly different between groups. Children in passive or active distraction group were more distracted than children in control group. Moreover, self-reported pain response by children($2.70{\pm}0.88$) and researcher($12.97{\pm}2.39$) in active group were higher than pain scores by children($2.27{\pm}0.64$) and researcher($10.63{\pm}1.65$) in passive group. Results of this study identified that distraction intervention is an effective method for decreasing pain responses in preschool children during immunization. Passive distraction is more effective than active distraction. Use of distraction strategies during immunization should be facilitated. Further research comparing distraction strategies by types and forms is needed.

Reproductive fecundity of Iraqi Awassi ewes immunized against synthetic inhibin-α subunit or steroid-free bovine follicular fluid

  • Al-Sa'aidi, Jabbar Abbas Ahmed;Khudair, Khalisa Khadim;Khafaji, Sura Safi
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.8
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    • pp.1169-1175
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    • 2018
  • Objective: The present study was conducted to investigate the impacts of active and passive immunization against synthetic inhibin and steroid-free bovine follicular fluid, respectively, on reproductive fecundity out of breeding season in Iraqi Awassi ewes. Methods: Follicular fluid was aspired from mature bovine follicles, treated with activated charcoal, and used for immunization of male rabbits for obtaining steroid free bovine follicular fluid (SFBFF) antiserum. Forty non-pregnant Awassi ewes were allocated into 4 groups (n = 10 each). At day 38 of experiment, ewes were treated with intra-vaginal MPA sponge (60 mg for 12 days). At days 0, 28, and 50, ewes were treated with 4, 2, and 2 mL of normal saline (control; C-ve), 400, 200, and $200{\mu}g$ of ovalbumin (C+ve), 400, 200 and $200{\mu}g$ of inhibin (SI group), respectively, and 4 mL of normal saline at day 0, and 4 and 2 mL of SFBFF antiserum at days 28 and 50, respectively, (AI group). After mating with Awassi rams, pregnancy and embryo number were diagnosed, at day 38 of pregnancy, using ultrasonography. Blood samples were collected at days 30, 60, 90, and 120 of pregnancy, for assessment of estradiol-$17{\beta}$ (E2) and progesterone (P4) levels. After parturition, numbers of delivered lambs were recorded. Results: The results revealed significant increase of P4 and significant decrease of E2 levels in SI and AI pregnant ewes than controls at days 30, 60, 90, and 120. Newborn number increased significantly in SI and AI treated than control ewes. Conclusion: Active or passive immunization against endogenous inhibin could augment reproductive fecundity out of breeding season in Iraqi Awassi ewes.

Production of Monoclonal Antibodies Specific to FimA of Porphyromonas gingivalis and Their Inhibitory Activity on Bacterial Binding

  • Koh, Eun-Mi;Kim, Ju;Lee, Jin-Yong;Kim, Tae-Geum
    • IMMUNE NETWORK
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    • v.9 no.5
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    • pp.203-207
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    • 2009
  • Background: The FimA of Porphyromonas gingivalis is a crucial pathogenic component of the bacteria and has been implicated as a target for vaccine development against the periodontal diseases. Methods: In this study, the purified fimbriae (FimA subunit polymers) protein was used for immunization in their native form and B hybridoma clones producing antibodies specific to FimA were established. Results: The monoclonal antibodies prepared from selected two clones, designated #123 (IgG2b/ kappa) and #265 (IgG1/kappa), displayed different patterns of binding activity against the cognate antigen. Both antibodies reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the monoclonal antibodies showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. Conclusion: These results suggest that the monoclonal antibodies could be used as vaccine material against the periodontal diseases through passive immunization.

The effect of active immunization with Acanthamoebn culbeksoni in mice born to immune mother (수동면역이 Acanthmoeba culbertsoni 능동면역 형성에 미치는 영향)

  • 공현호;서성아
    • The Korean Journal of Parasitology
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    • v.31 no.2
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    • pp.157-164
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    • 1993
  • Acanthamoebn culbertsoni is a pathogenic free-living amoeba causing primary amoebic meningoencephalltls (PAME) in human and mouse. Several reports on the immune responses in mice with this amoebic infection have been published, but the effects of transferred passive Immunity on the active immunization In offspring mice have not been demonstrated. This experiment was done to observe the effect of active Acanthamoebn culbertsoni was cultured in the CGV medium axenlcally. Female BALB/c mice weighing about 20g were immunized through the intraperitoneal injection of Acanthamoeba cuLbensoni trophozoites 1 × 106 each three times at the interval of one week. Offspring mice were immunized two times. The mice were inoculated Intranasally with 1 × 104 trophozoites under secobarbital anesthesia. There was a statistical difference in mortality between the transferred immunity group and the active immunization group. Statistical differences were not demonstrated in antibody titer between both groups. But L3T4+ T ce11/Ly2+T cell ratio was increased in the transferred Immunity group more than active immunization group of the offspring mice at the age of 5 weeks. There was no differences statistically in mortality between both groups. It was recognized that active immunization in offspring mice born to immune mother could modulate the immune status according to the time of Immunization.

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Effect of Dietary Protein Level on Immune Substances in Milk and its Transfer to Pups in Rats (흰쥐에서 식이 단백질 수준이 수유를 통한 수동면역에 미치는 영향)

  • 김화영
    • Journal of Nutrition and Health
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    • v.29 no.6
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    • pp.569-577
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    • 1996
  • This study was performed to determine the effect of maternal protein intake on 1) the concentration of immune substances in milk 2) degree of passive immunity to pups via lactation, and 3) specific antibody production to a specific antigen, $\beta$-lactoglobulin(BLG). 4) the effect of passive immunity that pups received from mother during lactation on the production of antibodies when the pups were challenged to the same antigen. Part of the female rats were immunized with BLG before and during pregnancy. The pregnant rats were placed into either 25% or 10% isolated soy protein diet throughout gestation and lactation. After weaning, pups from each group continued to be fed the same diet. At 18 weeks of age, all the pups were challenged with BLG. Total IgA and IgG, lysozyme, BLG-specific IgA and IgG were measured in dam's serum, dam's milk, and pup's serum. Total IgG, and lysozyme in dam's serum and milk were higher in high protein group. Total IgA and IgG in pup's serum remained higher in high protein group from 5 to 18 weeks of age. BLG-specific antibodies were found in the milk and serum of immunized dams, and in serum of pups born to immunized dams but not in the non-immunized group. BLG-specific IgA and IgG were again higher in high protein group and declined with time. The concentration decreased faster in the low proetein group than in the high protein groups. After immunization the pups with LBG, serum BLG-specific antibodies were not differ between rats born to immunized dams and those born to non-immunized dams. Therefore passive immunity rats received via milk as a pup had no effect on the BLG-specific antibody production later in life. This study shows the importance of protein status of mother and strongly support to the endorsement of breast feeding.

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Effect of Maternal Passive Autoimmunization against Myostatin on Growth Performance in Chickens

  • Moon, Y.S.;Lee, H.G.;Yin, Y.H.;Jin, X.;Hong, Z.S.;Cho, J.S.;Kim, S.C.;You, S.K.;Jin, D.I.;Han, J.Y.;Choi, Y.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.1017-1021
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    • 2005
  • Myostatin is a negative regulator of skeletal muscle growth and a loss of functional myostatin protein increases muscle hypertrophy and hyperplasia in cattle. The present study was conducted to investigate whether maternal passive immunization against myostatin would improve growth performance in chickens. A complete broiler myostatin cDNA was cloned and it was expressed into two transcripts as 1,128 bp and 985 bp by alternative splicing. A conjugated mature myostatin (350 bp) was used to induce autoimmunization and maternal passively immunized chickens was used for the experiment. It was confirmed that there was a maternal passive immunization against myostatin at zero weeks of age, but its effect was reduced by 6 weeks of age. The auto-immunized groups showed smaller body weights than those of control group during the growing period and the difference was getting bigger with time until 6 weeks of age. These results suggest that passive autoimmunization against myostatin used in this study is not potent enough to stimulate growth performance in chickens.

Antifertility Effect of Progesterone Antibodies in Mice

  • Yoon, Do-Young;Kim, Jae-Wha;Hwang, Soo-Weon;Choi, Myung-Ja;Choe, In-Seong;Kim, Jong-Bae;Chung, Tae-Wha
    • Archives of Pharmacal Research
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    • v.12 no.4
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    • pp.269-275
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    • 1989
  • Early embryo development and implantation were arrested in ICR mice which were passively immunized with a mouse monoclonal progesterone amtibody given as a single intraperitoneal injection at 12 hrs or 60 hrs post coitum (p. c.). Unimplanted embryos were recovered from the reproductive tract of the antibody-treated mice and none of these progressed to the blastocyst stage. The most pronounced effect was an arrest of embryonic development at a stage prior to cavitation. The plasma progesterone concentration in the blood taken by carbiac puncture increased greatly after the treatment by virtue of high affinity binding by the antibody in circulation. The result showed that passive immunization against progesterone shortly after mating interfered with early hormone dependent steps which were essential for normal embryonic development.

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