• Title, Summary, Keyword: PLHC-1 cell line

Search Result 1, Processing Time 0.021 seconds

Studies on Toxicological Evaluation of Freshwater Sediment using a PLHC-1 Cell Comet Assay (PLHC-1세포주의 Comet assay를 이용한 하천 퇴적토의 생태독성평가)

  • Bak, Jeong-Ah;Hwang, In-Young;Baek, Seung-Hong;Kim, Young-Sug
    • Korean Journal of Environmental Biology
    • /
    • v.29 no.1
    • /
    • pp.23-30
    • /
    • 2011
  • In this study, the Comet assay (evaluation of DNA damage) used the fish hepatocellular carinoma cell, PLHC-1, was tried to the sediment extract obtained from freshwater to understand its applicability as a tool for monitoring sediment toxicity. In parallel, induced EROD (7-ethoxyresorufin- O-deethylase) activity and DNA damage (TEM values) in PLHC-1 cells were measured for establishing the tandem endpoints of the PLHC-1cell test to test the ecotoxicity of sediment. Among several study sites in a small river passed through downtown and industrial park area, one of them, site B, showed a higher level of EROD activity and DNA damage than other sites. It indicates that a tandem endpoints of PLHC-1 cells could be useful tools for assessing the toxicity of sediment. The sensitivity of Comet assay with PLHC-1 cells was a little higher than that with a blood cell of frog tadpoles to the solvent extract of sediment. According to the results, a PLHC-1 cell-Comet assay could be used as a useful tool for evaluating ecotoxicity of the freshwater sediment. In addition, more detailed studies are needed to the contaminated site.