• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.12 no.4
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• pp.14-19
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• 1998

The purpose of our study is to develop tool kits which give us inexpensive simulation method. We develop a Programmable Logic Controller (PLC) program module with which we can edit and monitor PLC program, a virtual PLC (VPLC) system and simulation equipment. The PLC and simulation equipment are interlinked each other and controlled by the PLC program in real time. So, e can get effect as actural operation. Using our new tool kits we don't have to prepare PLC and machine, because we can configure PLC system and see the response of virtual machine n monitor. For validity of the developed program, we make experiments for the PLC program of elevator and automatic materials weighting system. The experimental results show that the virtual machine (VM) operates properly by the PLC control program.

• Journal of the Korean Institute of Telematics and Electronics T
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• v.36T no.1
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• pp.51-57
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• 1999

This thesis is written about the Wired Logic Type Unit in developing equipment that the load is driven, that the interface unit connect with 1 Scan time to PO30 of operated PLC output contact with that a sensing signal of Temperature Sensor RSD Pt $100\Omega$ let generate the relay output of temperature controller and input the PLC. The PLC Test Kit in country that the PLC to be programmed at the PLC education place is able to drive the load, is done to do a education of PLC on status that interface process between PLC and load are disregarded. As Developing Kit for supplement this point, when the relay output of temperature controller to use Pt 100 of temperature sensor as mentioned on the former among every kinds of sensor feed back to the input of the PLC, as the equipment to act with real time system that the output contact of PLC operated to insert the WLTIJ among PC, PLC and the load, it can understand and see very easy the main principle of PLC use. The structure of WLTU to be a interface unit of load separated as to the point of contact and noncontact, sensor, indicating lamp and A contact and B contact that is belong to driving part, and a motor is belong to loading part.

• Journal of the Korea Society for Simulation
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• v.19 no.2
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• pp.9-16
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• 2010

This study presents a system for validating the PLC code in the virtual environments of automobile trim line. There have been considerable efforts to develop standard PLC editors for saving human power and PLC test time. However, it was difficult to apply the PLC editor on a real shop floor environment. A virtual equipment was developed to communicate with the PLC module of a shop floor and to make up the connectivity environment by a special protocol. We can validate the code without launching real equipments because the PLC module and the virtual equipment is able to be controlled in real time by the OPC protocol. An experimental model is applied to the final assembly line of an automotive industry and validated by using DELMIA Automation. In conclusion, the system is valuable in the process of PLC codes validation and virtual equipments operation. This system would reduce the development time of the PLC codes and improve the productivity and the integrity of the PLC codes.

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.6
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• pp.349-355
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• 2003

We previously shown that LES contraction depends on $M_3$ receptors linked to PTX insensitive $G_q$ protein and activation of PLC. This results in production of $IP_3$, which mediates calcium release, and contraction through a CaM dependent pathway. In the esophagus ACh activates $M_2$ receptors linked to PTX sensitive $G_{i3}$ protein, resulting in activation of PLD, presumably, production of DAG. We investigated the role of PLC isozymes which can be activated by $G_q$ or $G{\beta}$ protein on ACh-induced contraction in LES and esophagus. Immunoblot analysis showed the presence of 3 types of PLC isozymes, $PLC-{\beta}1$, $PLC-{\beta}3$, and $PLC-{\gamma}1$, but not $PLC-{\beta}2$, $PLC-{\beta}4$, $PLC-{\gamma}2$, $PLC-{\delta}1$, and $PLC-{\delta}2$ from both LES and esophageal muscle. ACh produced contraction in a dose dependent manner in LES and esophageal muscle cells obtained by enzymatic digestion with collagenase. $PLC-{\beta}1$ or $PLC-{\beta}3$ antibody incubation reduced contraction in response to ACh in LES but not in esophageal permeabilized cells, but $PLC-{\gamma}1$ antibody incubation did not have an inhibitory effect. The inhibition by $PLC-{\beta}1$ or $PLC-{\beta}3$ antibody on Ach-induced contraction was antibody concentration dependent. The combination with $PLC-{\beta}_1$ and $PLC-{\beta}_3$ antibody completely abolished the contraction, suggesting that $PLC-{\beta}1$ and $PLC-{\beta}3$ have a synergism to inhibit the contraction in LES. $PLC-{\beta}1$, -${\beta}3$ or -${\gamma}1$ antibody did not reduce the contraction of LES cells in response to DAG ($10^{-6}$ M), suggesting that this isozyme of PLC may not activate PKC. When $G_{q/11}$ antibody was incubated, the inhibitory effect of the incubation of PLC ${\beta}3$, but not of PLC ${\beta}_1$ was additive (Fig. 6). In contrast, when $G_{\beta}$ antibody was incubated, the inhibitory effect of the incubation of PLC ${\beta}_1$, but not of PLC ${\beta}_3$ was additive. This data suggest that $G_{q/11}$/11 or $G{\beta}$ may activate cooperatively different PLC isozyme, $PLC{\beta}_1$ or $PLC{\beta}_3$ respectively.

• Yeungnam University Journal of Medicine
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• v.9 no.2
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• pp.288-301
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• 1992

The objective of the present study was to identify the characteristics of phospholipase C (PLC) isozymes purified from bovine brain and to investigate their interrelationship with G protein. The purified PLC isozymes ${\beta}$, ${\gamma}$ and ${\delta}$ were obtained and the characteristics of PLC activity on various concentrations of free $Ca^{2+}$ were observed. The activity of PLC was increased with increasing $Ca^{2+}$ concentration and the activity PLC ${\delta}$ was increased higher in the presence of phosphatidyl choline(PC) than in the abscence of PC. For vesicle formation as the structure of cell membrane, cholic acid and deoxycholic acid as detergent on phosphatidylinositol bisphosphate($PIP_2$) substrate containing PC were used, and then the activity of PLC isozymes were increased with increasing concentration of cholate, from 0.2% to 1% and were increased slightly in deoxycholate. In the $PIP_2$ containing phospholipid and glycolipid as brain extract, the activity of PLC isozymes were checked in 0.2%-1% cholic acid. The activities of PLC isoyzmes were continuously increased up to 1% cholic acid. The quantitation of PLC isozymes from several bovine organs by radioimmunoassay was made. Brain was the most sufficient organ in terms of amount of PLC ${\beta}$and ${\delta}$. A large amount of PLC ${\delta}$ was existed in adrenal gland. The binding capacity of GTPrS and G protein was observed and other observations of the binding effect of GTPrS-G protein and PLC monoclonal Ab-Protein A from tissue homogenate with PLC were made. From the observation the binding capacity was revealed the range of 0.11%-1.49%. The effects of each type of G protein on the percent activity of purified PLC isozymes were observed. From the observation, activities of isozymes were increased in $Go{\alpha}$ & Gmix, and the activities of PLC ${\beta}$ and ${\delta}$ were increased in $G{\beta}{\gamma}$ and $Gi{\alpha}$. Activities of PLC ${\beta}$ and ${\gamma}$ were decreased in $Gt{\alpha}$ but PLC ${\delta}$ increased.

• Proceedings of the Korean Society of Precision Engineering Conference
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• pp.140-143
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• 2000

This research presents new type of remote monitoring and control solution of PLC that can be used bi-directional and efficient management of factory automation through internet. This system has client/server architect for information handling between PLC and remote computer where a user can control and monitor target PLC. Actually the authors redirect RS232C connection between PLC and server computer into Internet connection between PLC and remote client computer using supplied PLC tool program So user feel like in front of PLC panel when he operates remote PLC through Internet. Each client/server program is constructed with Java language for security. In this paper the internet-based remote control system was proposed and proved validity by being applied to redirection of PLC control for factory automation.

• Korean Journal of Computational Design and Engineering
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• v.14 no.3
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• pp.176-185
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• 2009

Modern manufacturing systems consist of highly automated manufacturing devices, and they are controlled by complicated PLC programs. To make sure the achievement of the control objectives of a manufacturing system, it is very important to monitor the dynamic system behaviors of the manufacturing system. In this paper, we propose a monitoring methodology of a PLC program based on the Software In the Loop Simulation(SILS), which makes use of the time gap information between PLC signals. The errors relevant with PLC signals can be found using the proposed methodology, comparing a normal PLC signal trajectory with a target PLC signal trajectory. The proposed methodology has been implemented and tested with simple examples.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.40 no.5
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• pp.306-316
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• 2003

본 논문에서는 소프트웨어 PLC를 구현하는 방안을 제시하였다. 하드웨어는 표준적인 산업용 PC와 입출력 보드들로 구성된다. 운영체제로는 수십 마이크로초의 정밀한 단위로 태스크들의 스케줄링이 가능한 실시간 리눅스를 사용하였다. 소프트웨어 PLC를 위해 개발한 실시간 태스크들은 실시간 리눅스의 커널 모드에서 실행되며, 사용 목적에 따라 작성된 PLC 프로그램을 해석하고 실행하는 작업을 일정한 주기로 반복한다. 또한 PLC 프로그램을 편리하게 작성할 수 있도록 자체 프로그램 문법을 제안하고 이를 위한 컴파일러도 구현하였다. 이렇게 구현된 PLC는 가격 대비 성능이 우수하며 소규모의 응용분야들에 유용하게 활용할 수 있다.

• IE interfaces
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• v.18 no.4
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• pp.412-417
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• 2005

This paper describes the development of on-line Programmable Logic Controller(PLC) laboratories to teach the detailed operation of a PLC using distance learning approach. The PLC has become a key component to provide intelligence for machines in CIM environment. This study introduces a case study that teaches PLC programming in distance learning environment. The study describes the contents of PLC laboratories and workstation set ups. The study concludes with problems found and proposes the ways to improve the on-line laboratories.

• Journal of fish pathology
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• v.18 no.1
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• pp.67-80
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• 2005

Phosphoinositide-specific phospholipase $C\delta$ $PLC\delta$) plays an important role in many cellular responses and is involved in the production of second messenger. The present study was conducted to obtain the biochemical characteristics of the expressed recombinant $PLC\delta$ in E. coli cloned from Misgurnus mizolepis and partially purified $PLC\delta$ enzymes from liver tissues of M. mizolepis (wild ML-$PLC\delta$). The ML $PLC\delta$ gene was cloned and expressed under the previous report (Kim et al., 2004), and purified the recombinant protein by successive chromatography using $Ni^{2+}$-NTA affinity column and gel iltration FPLC column. The wild ML-$PLC\delta$ protein was solublized with 2 M KCI and purified by successive chromatography on open heparin-Sephagel and analytical TSKgel heparin-5PW. Both the recombinant and wild ML-$PLC\delta$ form of protein showed a concentration-dependent PLC activity to phosphatidylinositol 4,5-bis-phosphate (PIP$_2$) or phosphatidylinositol (PI). Its activity was absolutely $Ca^{2+}$- dependant, which was similar to mammalian $PLC\delta$ isozymes. Maximal PI-hydrolytic activations of recombinant and wild ML- TEX>$PLC\delta$ was at pH 7.0 and pH 7.5, respectively. In addition, the enzymatic activities of recombinant and wild ML-$PLC\delta$ were increased in concentration-dependent manner by detergent, such as sodium deoxycholate SDC), phosphatidylethanolamine (PE) and phosphatidylcholine (PC). The activities decreased in contrast by a polyamine, such as spermine. Western blotting showed that several types of $PLC\delta$ isozymes exist in various organs. Taken together our results, it suggested that the biochemical characteristics of ML-$PLC\delta$ are similar with those of mammalian $PLC\delta1$ and ${\delta}3$ isozymes.