• Title, Summary, Keyword: PLB

Search Result 54, Processing Time 0.041 seconds

Effect of Culture Medium, Temperature, and Light Intensity on PLB Propagation of Phalaenopsis (팔레높시스의 PLB 증식에 미치는 배지와 배양온도 및 광도의 영향)

  • 김미선;은종선;김재영
    • Korean Journal of Plant Tissue Culture
    • /
    • v.28 no.4
    • /
    • pp.215-219
    • /
    • 2001
  • This study was conducted to investigate the effect of culture media and environment on PLB proliferation by using PLBs produced from leaf segments excised from shoot of Phalanopsis flower stalk. The fresh weight of PLBs propagated was higher in MS medium than in NDM (New Dogashima medium) or VW, but the condition of PLB was better in NDM medium. Natural additives of Coconut water, potato and apple were absolutely required for the PLB propagation. PLB propagation was better in solid medium than in liquid medium including cotton as support. Optimal sucrose concentration for proliferation was 10 g/L. PLB proliferation was very effective condition 14.3 $\mu$mol.s$^{-1}$ m$^{-2}$ in PPFD and $25^{\circ}C$.

  • PDF

Molecular Cloning of a Putative Gene Encoding Phospholipase B (plbA) from Aspergillus nidulans (사상설 진균 Aspergillus nidulans의 Phospholipase B 유전자(plb A)의 클로링)

  • Hong, Sa-Hyun;Cho, Eun-Min;Song, Seung-Eun;Eom, Chi-Yong
    • Microbiology and Biotechnology Letters
    • /
    • v.36 no.3
    • /
    • pp.189-194
    • /
    • 2008
  • The phospholipase B (PLB) families are enzymes sharing phospholipase (PL), lysophospholipase (LPL) and lysophospholipase-transacylase (LPTA) activities. In this study, we report the putative gene encoding phospholipase B (plbA) containing lipase motifs was cloned for the first time from the filamentous fungus, Aspergillus nidulans. plbA was isolated from A. nidulans genomic DNA library using a PCR-amplified probe, which is designed on the basis of sequence information derived from the conserved lipase regions of various PLBs. The deduced product of plbA is of 626 amino acids. From the assigned sequence, PlbA showed 72% identity with Penicillium notatum PLB but have low similarity with phospholipase A of other organisms.

Quality Characteristics of the Bread Added with Prunus mume Byproduct Obtained from Liquer Manufacture (매실 리큐르 제조 부산물인 매실 과육을 첨가한 식빵의 품질 특성)

  • Chae, Myeung-Hee;Park, Na-Yeung;Jeong, Eun-Ju;Lee, Shin-Ho
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.35 no.9
    • /
    • pp.1267-1272
    • /
    • 2006
  • Effects of Prunus mume byproduct (PLB), obtained after manufacture of Prunus mume liquer, on quality characteristics of bread were investigated. The pH of dough and bread decreased but titratable acidity increased by addition of 10% or 20% PLB. The volume of dough during fermentation and the baking loss of bread containing PLB were smaller than those of the dough and bread without PLB. The weight of bread increased but the volume decreased by addition of PLB. L (lightness) value increased and a (redness) value decreased in the surface of bread containing PLB. b (yellowness) value did not show difference between treatments. The internal color of bread containing PLB decreased in L and a value but increased in b value. The hardness and strength of bread decreased with 10% PLB but increased with 20% PLB compared to control. Cohesiveness and springiness of bread increased with 10% or 20% PLB, but did not show significant difference between concentrations. As results of sensory evaluation, taste and flavor of the bread containing 10% PLB increased significantly compared to control. The sensory quality of bread with 20% PLB decreased significantly. The strength, springiness, and hardness of bread with 20% PLB were higher than those of control. The taste, flavor, color, and overall acceptability of bread with 10% PLB were better than those of control. No molds were found in breads with 20% PLB and 20% PLB powder during storage for 1 week at room temperature.

Effect of Maesil(Prunus mume) byproduct Obtained from Maesil Liqueur Manufacture on Kimchi Fermentation (매실 리큐르 제조 부산물인 매실의 첨가가 김치 발효에 미치는 영향)

  • Chae, Myeung-Hee;Park, Na-Young;Lee, Shin-Ho
    • Korean Journal of Food Preservation
    • /
    • v.13 no.6
    • /
    • pp.783-788
    • /
    • 2006
  • Quality changes of kimchi added with 10 or 20% Prunus mume liqueur byproduct (PLB), obtained after producing Prunus mume liqueur. during fermentation at $10^{\circ}C$ for 25 days were investigated. The pH and titrtable acidity in 20% PLB added kimchi were changed more gradually during fermentation for 25 days compared to control. Total bacteria and lactic acid bacteria counts in kimchi added with 20% PLB were lower than those of control during fermentation for 15 days. Kimchi fermentation was delayed about 10 days with 20% PLB. L and a values of kimchi added with PLB decreased but b value increased with increasing the concentration of PLB. In the sensory evaluation of kimchi fermented for 10 days, the texture score of PLB added kimchi was higher than that of control, and increased with increasing the concentration of PLB. There were no significant differences (p < 0.05) in overall acceptability among control kimchi and PLB added kimchi.

Optimization of efficient protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids

  • Soe, Khaing Wah;Myint, Khin Thida;Naing, Aung Htay;Kim, Chang Kil
    • Current Research on Agriculture and Life Sciences
    • /
    • v.32 no.4
    • /
    • pp.179-183
    • /
    • 2014
  • Optimization of the protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids was performed by determining the effects of plant growth regulators (PGRs) and different parts and division sizes of the PLB. For both genera, the base part was the best for the proliferation of PLBs, yielding the highest number of PLBs on a PGR-free medium for Phalaenopsis and medium containing $0.1{\mu}M$ NAA and $10.0{\mu}M$ BAP for Dendrobium. As regards the division size, four-division sections resulted in a higher PLB formation efficiency for Phalaenopsis, while two-division sections produced a higher PLB formation efficiency for Dendrobium. It is expected that these findings will be applicable to efficient PLB formation of other Phalaenopsis and Dendrobium orchids.

High Frequency Protocorm-like Body(PLB) Formation through Root Cultures Doritaenopsis Hybrids(Orchidaceae) (Doritaenopsis 뿌리배양으로부터 고빈도의 Protocorm-like Body(PLB)형성)

  • Park, So-Young;Oh, Sung-Rae;Paek, Kee-Yoeup
    • Journal of Plant Biotechnology
    • /
    • v.30 no.3
    • /
    • pp.241-244
    • /
    • 2003
  • Root cluster section culture, showing high efficient Protocorm-like body (PLB) formation capacity, were established in Doritaenopsis hybrids. Three types of root were obtained from excised shoots in 1/2MS medium containing different concentrations of NAA; \circled1normal roots, \circled2multiple roots and \circled3abnormal root clusters. Those were placed on 1/2MS medium supplemented with 0.5 mg/L thidiazuron for PLB regeneration. PLB regeneration rate was greater in root cluster section cultures (77.8%) compare to normal root tip cultures(30%). Number of PLBs regenerated from root cluster sections were counted over 11 per explant (5.3 per normal root tip).High frequency of PLB regeneration was achieved in root cluster section culture. This result can be used as an efficient method for clonal proliferation of Doritaenopsis hybrids.

Effect of Medium Composition on Phalaenopsis Micropropagation Using Lateral Buds From Flower Stalks (액아배양에 의한 호접란 대량번식시 배지조성의 영향)

  • Park, Myung-Joo;Park, Soon-Jung;Kim, Doo-Hwan
    • Horticultural Science & Technology
    • /
    • v.16 no.1
    • /
    • pp.42-44
    • /
    • 1998
  • The effect of medium composition on PLB formation and multiplication and shoot regeneration was studied to establish the micropropagation system of the tropical orchid Phalaenopsis. The highest frequence of PLB formation resulted from the VW medium with 1.2 times ion concentration, 1% sucrose, 1.5g/L PVP or 2.5g/L active charcoal, apple and potato extract and 4g/L gellan gum. The highest ratio of PLB multiplication was obtained from the VW medium with 2% sucrose, apple and potato extract and cotton plate. The shoot regeneration was the most effective with the hyponex medium with 3% apple, 3% potato and 4% banana extract.

  • PDF

Continuous Production of Phalaenopsis Clones by Basal Shoot Culture (호접란 줄기기저부 절편배양을 통한 조직배양묘의 연속생산)

  • Been, Chul-Gu
    • Journal of Plant Biotechnology
    • /
    • v.30 no.4
    • /
    • pp.375-380
    • /
    • 2003
  • This study was conducted to establish a practical masspropagation system of Phalaenopsis clones from basal shoot segments. The frequency of PLB (protocorm like body) induction was compared with various explants. Basal shoot segments showed the most successful result of 45%, while root tips, stalk node segments, stalk leaves and mature leaves represented low frequency (below 5%). The PLB induction ratio in the culture of basal shoot segments was examined with 11 different Phalaenopsis varieties, and the majority of varieties, including pink flower lines, showed an about 30% rate of PLB formation. Especially, when whole basal shoot parts without cutting were inoculated onto PLB induction medium, giant PLB was induced from explant. This giant PLB was green color and big in size compared with normal PLB. When dissected giant PLB segments inoculated onto PLB multiplication medium, only normal size of PLBs were induced from them. PLBs induced by basal shoot culture were transferred onto proliferation medium and then shooting medium, from which normal plants were formed. Therefore, this culture method is considered as effective and practical protocol for Phalaenopsis mericlone production. In addition, it is suggested that clones of an infinite number can be produced consecutively by this culture system through repeated cycles of PLB induction and proliferation using the basal shoot segment of flask plant.

Induction and Propagation of Protocom-Like Bodies from Shoot Tip Culture in the Pansy Orchid (Miltonia spp.) (경정배양에 의한 밀토니아의 PLB 유기와 기내 증식)

  • Kim, Mi-Seon;Hwang, Sun-Ja;Kim, Jae-Yeong;Choi, I-Jin
    • FLOWER RESEARCH JOURNAL
    • /
    • v.17 no.1
    • /
    • pp.9-14
    • /
    • 2009
  • This study was conducted to find out the commercial in vitro propagation methods through protocom-like bodies (PLB) induced from shoot tip culture of Miltonia spp. Among several culture media for induction PLB from shoot tip in Miltonia spp., MS basal medium was better than Hyponex, Vacin & Went basal medium and other media supplemented with natural additives. PLB's proliferation and differentiation in Hyponex medium including $20g{\cdot}L^{-1}$ banana + $20g{\cdot}L^{-1}$ sucrose(pH 5.2) was better than in MS medium. It was tendency that solid media showed higher PLB fresh weight than liquid medium or other cotton bridge culture. The dark culture for 1~2 weeks and adding $10{\sim}20g{\cdot}L^{-1}$ sucrose on hyponex basal medium was the most effective to increase the PLBs growth and shoot number.

In vitro Propagation and Protocorm-like Body Formation of Endangered Species, Dendrobium moniliforme (멸종위기 수종 석곡(Dendrobium moniliforme)의 Protocorm-like Body 형성 및 기내증식)

  • Bae, Kee Hwa;Kim, Nam Young;Song, Jae Mo;Song, Gwanpil
    • Journal of Forest and Environmental Science
    • /
    • v.30 no.1
    • /
    • pp.126-132
    • /
    • 2014
  • This experiment was conducted to establish the in vitro propagation of Dendrobium moniliforme through protocorm like body (PLB) induction from the culture of leaf, stem and root explants. Frequency of PLB formation from root explants was higher than those of leaf and stem explants. PLB's proliferation in MS medium including 1.0 mg/L NAA was better than 1.0 mg/L IBA. Frequency of PLB's proliferation on medium with various concentrations of BA, Kinetin, Tidiazuron (0. 1.0, 3.0 mg/L) and NAA (0, 1.0 mg/L) was tested. The maximun proliferation of PLB's was obtained on MS medium supplemented with 3.0 mg/L BA and 1.0 mg/L NAA after 6 weeks of culture. Addition of 200 mg/L activated charcoal (AC) and 30 g/L sucrose was effective on PLB proliferation for D. moniliforme.