• Title, Summary, Keyword: PAS-4

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Cloning of cDNA Encoding PAS-4 Glycoprotein, an Integral Glycoprotein of Bovine Mammary Epithelial Cell Membrane

  • Hwangbo, Sik;Lee, Soo-Won;Kanno, Chouemon
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.4
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    • pp.576-584
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    • 2002
  • Bovine PAS-4 is an integral membrane glycoprotein expressed in mammary epithelial cells. Complementary DNA (cDNA) cloning of PAS-4 was performed by reverse-transcriptase polymerase chain reaction (RT-PCR) with oligonucleotide probes based on it's amino terminal and internal tryptic-peptides. The cloned PAS-4 cDNA was 1,852 nucleotides (nt) long and its open reading frame (ORF) was encoded 1,413 base long. The deduced amino acid sequence indicated that PAS-4 consisted of 471 amino acid residues with molecular weight of 52,796, bearing 8 potential N-glycosylation sites and 9 cysteine residues. Partial bovine CD36 cDNA from liver also was sequenced and the homology of both nucleotide sequence was 94%. Most of the identical amino acid residues were in the luminal/extracellular domains. Contrary to PAS-4, bovine liver CD36 displays 6 potential N-glycosylation sites, which were located, except for those at positions 101 and 171, at same positions as PAS-4 cDNA. Cysteine residues of PAS-4 and CD36 were same at position and in numbers. Northern blot analysis showed that PAS-4 was widely expressed, although its mRNA steady-state levels vary considerably among the analyzed cell types. PAS-4 possessed hydrophobic amino acid segments near the amino- and carboxyl-termini. Two short cytoplasmic tails of the amino- and carboxyl-terminal ends constituted of a 5-7 and 8-11 amino acid residues, respectively.

Synthesis of Polyaminostyrene and Reverse Osmosis Characteristic of Inte4acially Polymerized Composite Membranes with Acyl Halides (Polyaminostyrene 합성 및 Acyl Halide와의 계면중합 생성 복합막의 역삼투 투과특성)

  • No, Il-Jun;Ha, Wan-Sik
    • Textile Science and Engineering
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    • v.33 no.11
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    • pp.947-954
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    • 1996
  • Poly(4-nitrostyrene) was synthesised by nitration of polystyrene and poly(3-ni-trostyrene) was synthesized by free radical bulk polymerization of 3-nitrostyrene. Polyaminostyrene(PAS) was synthesized by reduction of polynitrostyrene. Composite reverse osmosis(RO) membranes were fabricated via interfacial polycondensation of PAS and trimesoyl chloride(TMC) on the polysulfone support membrane surface. As the RO properties, salt rejection of poly(3-aminostyrene)(mPAS) membrane was better than poly(4-aminostyrene) (pPAS), but vice versa for permeate flux. The blend of 1, 3-phenylene diamine(mPDA) monomer in PAS reaction solution increased the salt rejection and the blend of acyl halide monomer in TMC reaction solution increased the permeate flux. The RO performance of mPAS and pPAS, in which various composition of the blended solutions were added, was found to be not much different. In particular, the PAS composite membrane which was prepared by interfacial polymerization of PAS/mPDA(70/30) blend solution and TMC/benzoyl chloride(80/20) blend solution showed a permeate flux of up to 1.0$\ell$/$m^2$.hr.atm and a salt rejection of over 90%.

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Sialoglycoproteins of Mammalian Erythrocyte Membranes: A Comparative Study

  • Sharma, Savita;Gokhale, Sadashiv M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1666-1673
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    • 2011
  • The presence of sialoglycoproteins (SGPs) in the membranes from goat (Capra aegagrus hircus), buffalo (Bubalus bubalis bubalis) and pig (Sus scrofa domestica) erythrocytes was investigated by partial purification with a chloroform-methanol extraction method followed by Sodium dodecyl sulphate - Polyacrylamide gel electrophoresis in comparison to human (Homo sapiens) erythrocytes. The results show that mammalian erythrocytes possess clear differences in the SGPs numbers and molecular weights although all animals studied in this experiment are from the same class i.e. mammalia. The SGPs number in human, goat, buffalo and pig are four (PAS-1 to PAS-4), ten (PAS-GI to PAS-GX), seven (PAS-BI to PAS-BVII) and four (PAS-PI to PAS-IV) respectively as indicated by staining the polyacrylamide gel with sialoglycoprotein-specific Periodic acid-Schiff's (PAS) stain. The new SGPs could be observed only after the partial purification of membrane fractions named as PAS-HI with molecular weight (Mr) 190 kDa and PAS-HII 150 kDa in human, PAS-BIA in buffalo and PAS-PIA and PAS-PIVA in pig. The gels were also stained with Coomassie brilliant blue (CBB) and Silver stain to check the contamination of other membrane proteins in the purified fractions. The quantitative distribution of SGPs was also determined by densitometry. Present study indicates that there are some basic differences in mammalian erythrocyte membrane SGPs, especially with respect to their number and molecular weights indicating major structural variations.

다양한 통신위성에 따른 KRISS와 NICT 시각비교

  • Han, Ji-Ae;Yang, Seong-Hun;Lee, Yeong-Gyu;Lee, Seung-U;Lee, Chang-Bok
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • v.2
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    • pp.490-492
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    • 2006
  • 본 논문에서는 현재 KRISS(한국표준과학연구원)에 구축 된 JCSAT-1B 위성과 PAS-8 위성 그리고 PAS-4 위성을 이용하여 KRISS와 NICT(일본의 정보통신연구기관)간의 양방향 시각비교를 통하여 그들의 안정도를 비교분석하고 향후 JCSAT-1B 위성의 백업시스템으로써 PAS-4 위성과 PAS-8 위성의 활용성을 검토하였다.

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Comparison of Aerodynamic Variables according to the Execution Methods of KayPENTAX Phonatory Aerodynamic System Model 6600 (KayPENTAX Phonatory Aerodynamic System Model 6600의 수행방법에 따른 공기역학 변수 비교)

  • Ko, Hyeju;Choi, Hong-Shik;Lim, Sung-Eun;Choi, Yaelin
    • Phonetics and Speech Sciences
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    • v.7 no.4
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    • pp.93-99
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    • 2015
  • In case of PAS test, the air is sometimes leaked although the mask is tightly attached to the face, which is not reliable on the measured values. Therefore, this study aimed to assist the clinical practice suggesting the test method of PAS without air leakage. In the healthy subjects with 12 males and 12 females over 19 years old, three types of tests were performed on the voicing efficiency among the protocol of PAS Model 6600. They are; first, to attach the mask tightly to the face holding the handle of PAS with the subject's two hands (Method 1); second, to attach the mask tightly to the face holding the handle of PAS with the subject's one hand and pushing the body of PAS strongly with the other hand (Method 2); and third, to attach the mask tightly to the face pushing the upper part of the mask by the tester when the subject attached the mask to his or her face holding the handle of PAS with two hands (Method 3). Upon the study analyses, the mean negative pressure, the mean phonogram, subglottic air pressure, and voicing efficiency were shown to be statistically significantly different during PAS test in males depending on the methods. (p<.05) In case of females, only the target airflow rate showed significant difference depending on the methods during PAS test. (p<.001) In conclusion, Method 2 enhanced the noise level and strength while Method 1 was likely to leak the air more compared to the other two methods in males. In case of females, Method 1 showed significant leakage of the air flow. Not to allow the air flow leakage without affecting the outcome of PAS test, it will be the most useful for the tester to push the mask to the subject's face tightly (Method 3).

Studies on Pharmaceutical Quantitative Analysis by means of Non-aqueous Titration Method (I) Isolative Determination of Mixed Weak Basic Chemicals. (비수적정법을 이용한 약품분석 연구 (제1보) 약염기성혼합약품의 분리정량법에 대하여)

  • 고인석;김재백;최병기
    • YAKHAK HOEJI
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    • v.5 no.1
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    • pp.20-23
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    • 1960
  • The study of the isolative determination of the mixed weak bases of INAH and NA-Pas potentimetrically considering the properties of solvents for the INAH and Na-PAS, dielectric constant and solvative properties of solvents are described. The methanol: dioxan (4:1) and glacial acetic acid: dioxan (4:1) are studied first as the mixed solvent, using the N/10 perchloric acid and glacial acetic acid solution as the titrant. The authors found that there is no inflection on INAH with the methanol: dioxan solvent system and on Na-PAS at glacial acetic acid dioxan solvent system. By applying methanol glacial acetic acid dioxan (1:1:1) solvent system, Na-PAS and INAH were successfully determined isolatively from the mixed sample, showing the distinguished inflections respectively as shown in the titration curves in figures 3 and 4. It is found that this method could save considerable time for the isolative determination of the mixed sample of week bases as Na PAS and INHA which were quite difficult to be determined by the present routine at control laboratory.

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Pain-relieving Effect of the PAS Release Applied to Tender Points (압통점에 적용한 파스이완술이 통증에 미치는 영향)

  • Park, Ji-Whan
    • The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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    • v.5 no.1
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    • pp.53-58
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    • 1999
  • PURPOSE; The main purpose of physical therapy is to minimize patient's pain. So this study aimed at evaluating the effect of PAS release applied to tender points in reducing pain of musculoskeletal lesion persons. METHOD; The PAS (capsicum plaster) Release applied at 48 tender points to relieve and assessment pain threshold by Harold Gottlieb's pain scale that was composed of Negligible sensation 4.00, Mild sensation 3.00, Moderate sensation 2.00, Severe sensation 1.00. RESULT; Results show that PAS release was 84.6% effect in achieving a gradual decrease of pain sensitivity at the tender points where it was applied, suggestion a cumulative analgesic effect through sessions. CONCLUSION; This study suggests that PAS release applied to tender points can be effective in relieving soft tissue pain through theses have not become asymptomatic, all referred significant pain relief(p <0.05) after study and at the end of PAS release therapy.

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A PAS-Containing Histidine Kinase is Required for Conidiation, Appressorium Formation, and Disease Development in the Rice Blast Fungus, Magnaporthe oryzae

  • Shin, Jong-Hwan;Gumilang, Adiyantara;Kim, Moon-Jong;Han, Joon-Hee;Kim, Kyoung Su
    • Mycobiology
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    • v.47 no.4
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    • pp.473-482
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    • 2019
  • Rice blast disease, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most important diseases in rice production. PAS (period circadian protein, aryl hydrocarbon receptor nuclear translocator protein, single-minded protein) domains are known to be involved in signal transduction pathways, but their functional roles have not been well studied in fungi. In this study, targeted gene deletion was carried out to investigate the functional roles of the PAS-containing gene MoPAS1 (MGG_02665) in M. oryzae. The deletion mutant ΔMopas1 exhibited easily wettable mycelia, reduced conidiation, and defects in appressorium formation and disease development compared to the wild type and complemented transformant. Exogenous cAMP restored appressorium formation in ΔMopas1, but the shape of the restored appressorium was irregular, indicating that MoPAS1 is involved in sensing the hydrophobic surface. To examine the expression and localization of MoPAS1 in M. oryzae during appressorium development and plant infection, we constructed a MoPAS1:GFP fusion construct. MoPAS1:GFP was observed in conidia and germ tubes at 0 and 2 h post-infection (hpi) on hydrophobic cover slips. By 8 hpi, most of the GFP signal was observed in the appressoria. During invasive growth in host cells, MoPAS1:GFP was found to be fully expressed in not only the appressoria but also invasive hyphae, suggesting that MoPAS may contribute to disease development in host cells. These results expand our knowledge of the roles of PAS-containing regulatory genes in the plant-pathogenic fungus M. oryzae.

Analysis of Sugar Chain Structure of PAS-7 Glycoprotein from Bovine Milk Fat Globule Membrane by US RAAM 2000 (OGS RAAM2000을 이용한 유지방구막 PAS-7 당단백질의 당쇄구조 해석)

  • 석진석
    • Food Science of Animal Resources
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    • v.21 no.4
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    • pp.367-373
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    • 2001
  • Glycoproteins PAS-6(50 kDa) and -7(47 kDa) from the bovine milk fat globule membrane share a common protein core but differ in their carbohydrate moiety. We have analyzed and proposed the structures of the N-linked sugar chains of PAS-7 by Oxford Glyco System(OGS) RAAM2000. The N-linked sugar chains were liberated from PAS-7 by hydrazinolysis and, after modifying the reducing ends with 2-aminobenzamide(2-AB), were separated into one neutral(7N, 55%) and two acidic(7M, mono-, 43%; 7D, di-, 2%) sugar chain groups. 7N was finally separated into 5 chains(a, b, c, d, and e), respectively. The structure of this 2AB-neutral sugar chain was determined by sugar analysis, exoglycosidase digestion with OGS glycosidase Kit and OGS RAAM2000 system. The results show that fraction e was the same of reported 7N1A, the biantennary complex type with a fucose on reducing end and two N-acetyllactosamine branch on non-reducing end. Therefore, it was proved that OGS RAAM2000 method is in conformity with conventional analysis of sugar chain structure from bovine PAS-7.

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Effects of Progesterone and 17β-Estradiol under Presence or Absence of FBS on Plasminogen Activators Activity in Porcine Uterine Epithelial Cells

  • Hwangbo, Yong;Lee, Mi-Rim;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Development and Reproduction
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    • v.22 no.4
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    • pp.309-318
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    • 2018
  • The present study was conducted to investigate the regulatory mechanism of plasminogen activators (PAs) activation by $17{\beta}$-estradiol ($E_2$) and progesterone ($P_4$) in porcine uterine epithelial cells (pUECs). pUECs were collected from porcine uterine horn and cultured at 80% confluence. Then, 0.1% (v/v) DMSO, 20 ng/mL $E_2$, and $P_4$ with or without fetal bovine serum (FBS) treated to cultured cells for 24 hours. The supernatants were used for measurement of PAs activity and expression of urokinase-type PA (uPA), tissue-type PA (tPA), uPA specific receptor (uPAR), and type-1 PA inhibitor (PAI-1) mRNA were analyzed by real-time PCR. The expression of PAs-related genes was not affect by steroid hormones in both of serum treatment groups. However, PAs activity was increased by treatment of $E_2$ compared to 0.1% DMSO treatment in serum-free group (p<0.05). Then, $E_2$ and $P_4$ were diluted with 0.002% (v/v) DMSO for reduction of its effect and treated to cultured cells without FBS. Only tPA mRNA was significantly increased by $E_2$ treatment (p<0.05). PAs activity was enhanced in $E_2$ treated group compared to control groups (p<0.05). These results indicate that serum-free condition is more proper to evaluate effect of steroid hormones and activation of PAs in pUECs was mainly regulated by estrogen. These regulation of PAs activation may be associated with uterine remodeling during pre-ovulatory phase in pigs, however, further studies are needed to investigate precise regulatory mechanism.