• 제목, 요약, 키워드: Organ indexes

검색결과 17건 처리시간 0.036초

오마환(烏麻丸)이 백서(白鼠)의 혈액내(血液內) CD4+, CD8+ T cell 및 면역기관(免疫器官) 장기지수(臟器指數)에 미치는 영향(影響) (Effects of Omahwan(OMH) on CD4+, CD8+ T cell and Immune Organ Index in Rat)

  • 이송실;이상재;김광호
    • 대한예방한의학회지
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    • v.5 no.1
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    • pp.76-89
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    • 2001
  • OMH which is known for its properties of recruiting vitality, is composed of Polygonum multiflorum Thunb. and Sesamum indicum DC.. This formula is known to possess the properties of recruiting vitality, blackening white hair and expanding life span. 16-week-old SD-Rats were treated with OMH for 16 days. After 24 hours, the rats were treated with MTX(Mthotrexate is oral administrated for 4 days(1mg/kg/day), in order to lower immunity. Then, These rats were classified in to groups, the N-16 group(not specially tested), the Control group(MTX), the OMH-L group(2.5% OMH+MTX)&the OMH-H group(10% OMH+MTX) and 6 rats were assigned to each group. After 18 hours from MTX treatment the organ index of the rats from each group Thymus and Spleen were measured. The percentage of CD+4, CD8+ T cell were measured and compared by flow cytometer. 1) Rats from the OHM-L&OMH-H group showed higher organ indexes of the Thymus and Spleen compared to the rats from the Control Group. This proves that OMH possesses the properties to mitigate degenerations of immunity($F_{thymus}=20.162,\;F_{spleen}=5.882$, ANDVA, p<0.05). 2) The rats from the two OMH groups showed higher rates of CD4+ T cell counts compared to the control group(F=26.906, ANOVA, p<0.05). CD8+ T cell showed lower rates compared to the Control group, but showed no differences within the two OMH groups(F=1.254, ANOYA, p>0.05). CD4+/CD8+ showed higher rates in the two OMH groups compared to the control group which can be thought as a proof that OMH prevents depression of immune response(F=10.554, ANOVA, p<0.05). In this test 16 week-old rats were used, which can be considered as the middle and prime age of the human being. These rats were treated with OMH which ended up showing properties of mitigating degeneration of immune responses and maintaining T-cell rates within the blood. It was possible to study that OMH possesses the properties to increase immune responses.

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The effects of paeoniflorin injection on soluble triggering receptor expressed on myeloid-1 (sTREM-1) levels in severe septic rats

  • Liu, Xiao-Rong;Xu, Jie;Wang, Yi-Min;Ji, Ming-Suo;Liu, Fu-Shan
    • The Korean Journal of Physiology and Pharmacology
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    • v.20 no.6
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    • pp.565-571
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    • 2016
  • Paeoniflorin (PAE) is the most abundant compound in Xuebijing injection widely used to treat sepsis. We aimed to investigate effect of PAE on expression of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in a rat model of sepsis. Wistar rats were divided into Normal, Model, and PAE groups (n=20 each). Endotoxin was administrated at 5 mg/ml/kg in Model and PAE rats to establish rat sepsis model. 1 h after endotoxin administration, PAE was administrated at 4 ml/kg in PAE group once per day for 3 days. Routine blood tests and biochemical indexes were assessed, including aspartate aminotransferase (AST) and creatine kinase-MB (CK-MB). The plasma sTREM-1 level was measured using quantitative ELISA. At the end of experiment, the small intestine, liver, kidney and lung were subjected to pathological examinations. A rat model of sepsis-induced multiple organ dysfunction syndrome (MODS) was established successfully with endotoxin administration (5 mg/ml/kg), evidenced by histo-pathological examinations, routine blood tests and biochemical indexes: platelet count decreased and white blood cell count increased (p<0.05), CK-MB and AST increased (p<0.05). PAE treatment significantly reduced the plasma levels of AST, CK-MB, and sTREM-1, compared to Model group (p<0.05). Meanwhile, sepsis-induced damages in the liver, lung, stomach and intestinal mucosa were also markedly ameliorated by PAE treatment. PAE demonstrated a significantly protective effect in a rat model of sepsis by decreasing plasma sTREM-1 level, reducing inflammation, preventing MODS and protecting organ functions.

Effect of Yeonsan Ogye bioactive peptides on anti-oxidant indexes in rats' liver

  • Kim, Hye Won;Shim, Jung Hun;Kim, Ki Nam
    • Journal of Nutrition and Health
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    • v.52 no.4
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    • pp.408-411
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    • 2019
  • Purpose: This study investigated the effect of bioactive Yeonsan Ogye peptides (YOPs) intake on changes in the hepatic anti-oxidant indexes in male rats. Methods: Sprague-Dawley male rats were divided into 3 groups and given a casein-based AIN-93G diet and distilled water ad libitum without any added YOPs (control), distilled water with 250 mg of YOPs (Y250), or 500 mg of YOPs (Y500) per kg of body weight for 4 weeks. YOP dose was decided as referred to in the referenced study where toxicity did not occur. The hepatic anti-oxidant indexes were determined using a commercial kit. Statistical analysis was performed using SPSS version 23.0 and are expressed as $mean{\pm}standard$ error of mean. Differences among the groups were evaluated by one-way analysis of variance followed by post hoc Duncan's multiple comparisons test. Results: There were no differences in the body weights, weight gain, food intake, food efficiency ratio, or organ weight, including liver, kidney, spleen, thymus, and epididymal fat, among all of the groups. The hepatic nitric oxide (NO) level in the Y500 group was lower than that in the control and Y250 groups, and the hepatic malondialdehyde (MDA) level was lower in the Y500 group than in the Y250 group. The differences in hepatic superoxide dismutase (SOD) and catalase (CAT) activities were not statistically significant between the groups. From these results we speculated that YOPs may have anti-oxidative abilities to regulate NO and MDA production without affecting SOD and CAT activities. Conclusion: YOPs are presumed to act as anti-oxidants in the animal or human body.

AHP방법을 적용한 국립검역소 평가 준거의 가중치 결정 (Determining Relative Weights of Criteria for Evaluating National Quarantine Station by the Analytic Hierarchy Process)

  • 정문용;이무식;김대경;유인숙
    • 한국산학기술학회논문지
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    • v.11 no.1
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    • pp.335-340
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    • 2010
  • 신종 및 재출현 전염병이 지속적으로 유행하고 해외 전염병의 국내유입 가능성이 증대됨에 따라 국립검역소의 검역관리 사업의 효율성 평가는 매우 중요하다. 본 연구는 평가 구성요소 사이의 상대적 중요도, 즉 가중치(weight) 부여에 있어서 과학적 타당성을 인정받고 있는 계층분석절차(Analytical Hierarchy Process; AHP) 기법에 의한 이원비교방법을 사용하여 설정하였다. AHP기법은 복잡한 다기준 의사결정문제(multi-criteria decision making problem)를 계층화하여 단순화 체계화시킴으로써 그 영향도를 계량화하는데 탁월한 기법이라는 평가를 받고 있다. 평가지표별 가중치는 기관평가 영역이 0.2, 서비스 및 프로그램 영역이 0.8로 분석되었으며, 서비스 및 프로그램 영역내에서 검역 업무가 0.45로 가장 높게 나타났다. 대영역(기관평가영역, 서비스 및 프로그램평가 영역), 중영역(투입, 과정, 결과, 검역업무, 검사업무, 위생관리업무, 병원체조사감시업무, 전염병예방홍보 교육업무)으로 구분하여 최종적으로 평가지표로 선정된 지표는 실제 국립검역소 사업에 적용하였으며, 이에 대한 최소한의 평가지표를 최종 선정하여 향후 국립검역소 사업평가 체계를 보다 체계화 하였다.

양심탕(養心湯)이 스트레스와 면역기능(免疫機能)에 미치는 영향(影響) (The Effects of Yangsimtang on Stress and Immune System)

  • 윤상희;이상용
    • 동의신경정신과학회지
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    • v.7 no.1
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    • pp.49-63
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    • 1996
  • After applying the gravity acceleration stress to a mice, the effect on organ index was examined, and the Con A stimulating proliferation rate of splenocytes, expression of IL-2 receptor and T cell subsets of thymocytes were analyzed and also clearance of C. neoformans was measured. The results were as follows :1. Form finding the organ index after 4 days stress, the indexes of the spleen and thymus were reduced in the res group exposed to the gravity acceleration.2. From finding the proliferation rate by stimulating the splenocytes with Con 4 after 7 days stress, the proliferation rates were all reduced in the stress group, the Yangsimtang group, and the stress and Yangsimtang group. 3. The expression of IL-2 receptor in resting stage was reduced, comparing to the test group, both in the stress group and the Yangsimtang group, however, comparing to the stress group, it was somewhat recovered in the stress and Yangsimtang group.4. To see the IL-2 receptor driven-out after being stimulated by Con-A, the expression of IL-2 receptor was all reduced in the stress group, the Yangsimtang group, and the stress and Yangsimtang group.5. To the rate of T cell subsets of thymus, there's no difference, comparing to the test group, in the Yangsimtang group, however, the rate of $CD4^+CD8^-,\;CD4^-CD8^+,\;and\;CD4^-CD8^-$ cell was significantly reduced in the stress group. And, the $CD4^+CD8^+$ which had been reduced by stress was somewhat recovered in the stress and Yangsimtang group.6. To the effect on the clearance of C, neoformans infection, the numbers of fungi detected at the spleen was, comparing to the test group, increased by 12.6 tines in the Yangsimtang group.

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Feeding of Dehulled-micronized Faba Bean (Vicia faba var. minor) as Substitute for Soybean Meal in Guinea Fowl Broilers: Effect on Productive Performance and Meat Quality

  • Tufarelli, Vincenzo;Laudadio, Vito
    • 아세아태평양축산학회지
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    • v.28 no.10
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    • pp.1471-1478
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    • 2015
  • The present study aimed to assess the effect of dietary substitution of soybean meal (SBM) with dehulled-micronized faba bean (Vicia faba var. minor) in guinea fowl broilers on their growth traits, carcass quality, and meat fatty acids composition. In this trial, 120 day-old guinea fowl keets were randomly assigned to two treatments which were fed from hatch to 12 weeks of age. Birds were fed two wheat middlings-based diets comprising of a control treatment which contained SBM (78.3 g/kg) and a test diet containing dehulled-micronized faba bean (130 g/kg) as the main protein source. Substituting SBM with faba bean had no adverse effect on growth traits, dressing percentage, or breast and thigh muscles relative weight of the guinea fowls. Conversely, a decrease (p<0.05) of abdominal fat was found in guinea fowls fed the faba bean-diet. Breast muscle of birds fed faba bean had higher $L^*$ score (p<0.05) and water-holding capacity (p<0.05) than the SBM control diet. Meat from guinea fowls fed faba bean had less total lipids (p<0.05) and cholesterol (p<0.01), and higher concentrations of phospholipids (p<0.01). Feeding faba bean increased polyunsaturated fatty acid concentrations in breast meat and decreased the saturated fatty acid levels. Moreover, dietary faba bean improved the atherogenic and thrombogenic indexes in guinea fowl breast meat. Results indicated that substitution of SBM with faba bean meal in guinea fowl diet can improve carcass qualitative traits, enhancing also meat lipid profile without negatively affecting growth performance.

Beneficial Effect of Anti-obese Herbal Medicine Mixture with Chitosan in High Fat Diet-induced Obese Rats

  • Beik, Kyung-Yeun;Lee, Sang-Il;Kim, Soon-Dong
    • Preventive Nutrition and Food Science
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    • v.14 no.4
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    • pp.290-297
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    • 2009
  • This study was carried out to investigate the dietary effects of chitosan mixture (CM), an herbal medicine mixture with Sukjihwang (HS), and CM containing HS (CHS) on obesity in an induced obese model of rats fed high-fat only (HF), in which supplemented diets of 5% CM (HCM), 5% HS (HHS), or 2.5% CM-2.5% HS (HCH) was tested for 6 weeks. Body weight gains, obesity indexes, and body fat contents in the experimental groups (HCM, HHS, HCH) were decreased compared with HF group. The levels of serum triglyceride, total lipid, total cholesterol and LDL-cholesterol in the experimental groups were markedly decreased, however HDL-cholesterol levels in the experimental groups were slightly increased compared with HF group. In addition, although serum ALT and AST activity, and relative organ weights were lower than those of HF group, serum albumin contents were not significantly different in all experimental groups including the normal control group (NC). In conclusion, there are improved effects on obesity in the obese model of animals with all experimental diets supplementations, and the improvement degrees on obesity depend on the content and compositions of the herbal medicine mixture. Further study is needed on the anti-obesity mechanism of these diets.

안존이천탕 추출물이 흰쥐의 모체 및 태자에 미치는 영향 (The Toxicological Effects of Ahnjon-Yichun-Tang in Pregnant Rats and Fetuses)

  • 김범회
    • 대한예방한의학회지
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    • v.17 no.2
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    • pp.157-168
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    • 2013
  • The objective of this study was to characterize the adverse effects of Ahnjon-Yichun-Tang during early pregnancy. Following successful mating, female Sprague-Dawley rats were given Ahnjon-Yichun-Tang(AYT) extract by oral administration daily with dose of 150mg (n=10), 300mg(n=10), 450mg(n=10) for 20 days of pregnancy. The rats in Control group(n=10) were orally administrated with Saline. All pregnant rats of Ahnjon-Yichun-Tang-treated and Control groups were sacrificed on day 20 of pregnancy. The pregnancy outcome was determined and the internal and reproductive organs of pregnant rat were observed. The fetuses were examined for the presence of various developmental toxic endpoints and stained with alcian blue and alizarin red S, and observed skeletal malformations. The results obtained in this study represent that there is no significant changes between Control and Ahnjon-Yichun-Tang-treated groups in body weight, organ weight, blood chemistry values, hematological values and pregnancy indexes of pregnant rat. The skeletal malformation of fetus was not observed as well. These results suggest that oral administration of Ahnjon-Yichun-Tang does not produce either maternal or developmental toxicity.

Protective effects of Korean red ginseng extract on cadmium-induced hepatic toxicity in rats

  • Park, Sook Jahr;Lee, Jong Rok;Jo, Mi Jeong;Park, Sang Mi;Ku, Sae Kwang;Kim, Sang Chan
    • Journal of Ginseng Research
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    • v.37 no.1
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    • pp.37-44
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    • 2013
  • Korean red ginseng is known to regulate the immune system and help the body struggle infection and disease. Cadmium is widely distributed in the environment due to its use in industry. Exposure to cadmium is problematic causing organ dysfunction. This study was conducted to evaluate the protective effect of Korean red ginseng extract (RGE) against cadmium-induced hepatotoxicity in rats. In experiments, animals were orally administrated with RGE (25, 50 mg/kg) for 7 d and then intravenously injected with cadmium ($CdCl_2$, 4 mg/kg) to induce acute hepatotoxicity. Cadmium caused the elevated levels of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase in serum. In contrast, pretreatment with RGE significantly reduced those serum indexes related with liver damage. In histopathological analysis, RGE decreased the centrilobular necrosis around central veins and the peripheral hemorrhage around portal triads. Moreover, RGE restored the deficit in hepatic glutathione level resulting from cadmium treatment. RGE also inhibited the increase in the expression of Bad, a representative apoptosis marker protein, induced by cadmium treatment. Collectively, these results demonstrate that RGE can reduce the cadmium-induced hepatic toxicity, partly via anti-oxidative and anti-apoptotic process.

능이 버섯이 고지방식이에 의한 고지혈증 비만 쥐에 미치는 영향 (Effects of the Sarcodon aspratus on the High Level of Blood Lipid and Obesity Induced by High Fat-diet in Rat)

  • 이경숙;김종봉
    • 생명과학회지
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    • v.19 no.9
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    • pp.1265-1270
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    • 2009
  • 능이버섯의 항비만 건강식품으로서의 이용가능성을 분석하였다. 고지방식이에 의해 유도된 비만쥐에 6주 동안 능이버섯 분말을 섭취시켜, 능이버섯의 효과를 알아보기 위하여 비만지수, 혈청지질, 장기무게 등의 여러 가지 요인들을 분석하였다. 고지방식이를 먹인 쥐 혈청의 중성지방, 총콜레스테롤 및 Ldl은 유의적으로 증가하였으나 능이버섯분말을 먹인 쥐는 증가하지 않았다. 고지방식이 쥐에서 R$\ddot{o}$hrer index는 대조군에 비해 유의적으로 증가하였으나 버섯 분말을 섭취한 쥐는 R$\ddot{o}$hrer index가 유의적으로 감소하였다. 특히 몸무게에 대한 간 무게의 비율은 대조군에 비해 비만식이 쥐에서 높았다. 이러한 결과들은 버섯을 먹인 쥐에서의 비만지수와 장기무게의 유의적인 변화는 혈청에서 중성지방의 감소 때문인 것으로 생각된다. 그렇기 때문에 능이버섯은 비만과 관련된 질병을 막을 수 있는 건강식품의 하나로 이용 될 수 있는 가능성이 있다.