• Title, Summary, Keyword: ODME

Search Result 3, Processing Time 0.02 seconds

Effect of Several Species of the Family Rubiacea on Cytotoxicity and Apoptosis in HL-60 cells

  • Ju Sung-Min;Lee Jun;Choi Ho-Seung;Kim Sung-Hoon;Jeon Byung-Hun
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.20 no.1
    • /
    • pp.187-192
    • /
    • 2006
  • Herbal medicines have been utilized to treat a variety of diseases, including cancer. Several species of the family rubiaceae have been reported to have antitumor activity. In this study, we report the cytotoxicity and antitumor activity exhibited dy the methanol extracts prepared from Rubia radix (RRME), Uncaria gambir (UGME) and Oldenlandia diffusa (ODME) (family: Rubiaceae) against human promyleloid leukemia cell line, HL-60. The cytotoxicity of RRME (2~20 ${\mu}g/ml$), UGME (20~200 ${\mu}g/ml$) and ODME (20~200 ${\mu}g/ml$) were assessed dy the MTT reduction assay. IC50 values for RRME, UGME and ODME were 11.0, 99.5 and 106.1 ${\mu}g/ml$, respectively. When the HL-60 cells were treated with RRME (10 ${\mu}g/ml$), UGME (120 ${\mu}g/ml$) and ODME (140 ${\mu}g/ml$) for 24 h, several apoptotic characteristics such as DNA fragmentation and morphologic changes were observed. Furthermore, flow cytometric analysis was peformed to determine the percent of apoptotic cells. The poupulation of sub-G1 hypodiploid cells was increased 37.49% in RRME treatment, 12.49% in UGME treatment and 7.21% in ODME treatment compared with untreated control cells (2.64%). To further confirm apoptotic cell death, we assayed caspase-3, -8 and -9 activities in RRME, UGME and ODME-treated cells. After treatment of RRME, UGME and ODME for 12 h, caspase-3, -8 and -9 activities significantly increased.compared to untreated control cells. These results show that RRME, UGME and ODME induced apoptotic cell death in HL-60 cells and may have a possibility of potential antitumor activities.

A Design of Oil Content Meter Using Scattered Light Turbidity Method for Oil Discharge Monitoring Equipment (선박용 유배출 감시 장치를 위한 광산란 탁도 방식의 유분 농도계 개발)

  • 이승희;양구주;장용석;김재형
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • /
    • pp.583-587
    • /
    • 1998
  • 본 논문에서는 대형 탱커선의 의무 장비인 유배출 감시 장비 (Oil Discharge Monitoring Equipment)를 위한 유분 농도계의 설계에 대하여 연구하였다. ODME는 국제해사기구 (IMO)에서 일정 ton 이상의 대형 선박에 의무적으로 설치하도록 규정하고 있으며 이는 날로 심각해지는 해양 오염을 방지하는데 목적이 있다. ODME의 구성 요소는 중앙 제어 장치, 유분 농도계, 유량계, 선속계, Sampling System, 배출 제어 장치로 이루어져 있으며 이중 가장 중요한 것은 유분 농도계이다. 이는 선박에서 배출되는 물에 포함된 유 성분을 ppm 단위로 계측하는 것이다. 이를 위한 탁도 측정 방법에는 여러 가지가 있지만, 본 연구에서는 선박에서 사용이 용이한 광산란 방식과 방폭성을 가지는 광섬유를 이용하여 탁도를 측정하는 유분 농도계를 설계하였으며 아울러 이에 대한 탁도 계산 알고리즘에 대하여 고찰하였다. 측정의 정밀도는 0-999ppm 범위에서 $\pm$5%의 오차 이내로 측정이 가능함을 보였다. 이는 IMO의 규격 ($\pm$10%의 오차)에 만족하는 수치이다.

  • PDF

On-line Prediction Model of Oil Content in Oil Discharge Monitoring Equipment Using Parallel TSK Fuzzy Modeling (병렬구조 TSK 퍼지 모델을 이용한 선박용 기름배출 감시장치의 실시간 기름농도 예측모델)

  • Baek, Gyeong-Dong;Cho, Jae-Woo;Choi, Moon-Ho;Kim, Sung-Shin
    • Journal of Institute of Control, Robotics and Systems
    • /
    • v.16 no.1
    • /
    • pp.12-17
    • /
    • 2010
  • The oil tanker ship over 150GRT must equip oil content meter which satisfy requirements of revised MARPOL 73/78. Online measurement of oil content in complex samples is required to have fast response, continuous measurement, and satisfaction of ${\pm}10ppm$ or ${\pm}10%$ error in this field. The research of this paper is to develop oil content measurement system using analysis of light transmission and scattering among turbidity measurement methods. Light transmission and scattering are analytical methods commonly used in instrumentation for online turbidity measurement of oil in water. Gasoline is experimented as a sample and the oil content approximately ranged from 14ppm to 600ppm. TSK Fuzzy Model may be suitable to associate variously derived spectral signals with specific content of oil having various interfering factors. Proposed Parallel TSK Fuzzy Model is reasonably used to classify oil content in comparison with other models. Those measurement methods would be effectively applied and commercialized to oil content meter that is key components of oil discharge monitoring control equipment.