• Title, Summary, Keyword: Nanoenzyme

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Development of Nanoenzymes for the Production of Glucose from Seaweed and Various Polysaccharide (해조류 및 다당류로부터 포도당 생산을 위한 나노효소 개발 및 특성)

  • Jin, Lie-Hua;Lee, Jung-Heon
    • KSBB Journal
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    • v.25 no.5
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    • pp.453-458
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    • 2010
  • The magnetically separable polyaniline nanofiber enzymes were developed for the recycle of enzyme and enhanced enzyme stability. The stability of enzyme was maintained over 90% for 8 days under room temperature and vigorous shaking conditions (200 rpm). The residual activity of immobilized enzyme was over 60% after 8 days incubation at $55^{\circ}C$. Glucose was produced from various polysaccharides, agarose, curdlan, cellulose, and sea weed, using magnetically separable immobilized enzyme. Glucose production rate with curdlan was 1.2 g/(l h) and showed high decomposition rate due to high mass transfer. After 10 times recycle, the residual activity of immobilized enzyme was over 75%. 1 g/L of glucose was produced with 5 mg of immobilized enzymes.

Development of (α-Amylase Coated Magnetic Nanofiber for the Hydrolysis of Starch. ((α-Amylase가 고정화된 Magnetic Nanofiber를 이용한 전분 분해공정 개발)

  • Kim, Hyun;Lee, Jung-Heon
    • Journal of Life Science
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    • v.17 no.9
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    • pp.1260-1265
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    • 2007
  • Magnetically separable enzyme-coated nanofibers were developed for the hydrolysis of starch. Stability of ${\alpha}-amylase-coated$ nanofiber was greatly improved and its residual activity was maintained over 92.7% after 32 days incubation at room temperature and under shaking conditions (200 rpm). The recovery of enzyme was high and enzyme activity after 10 recycle was 95.2% of its original activity. Developed enzyme-coated nanofibers were used for the hydrolysis of starch. When 0.5 mg of magnetically separable enzyme nanofibers was used, 40 g/l of starch (2 ml) was completely degraded within 40 min. The continuous enzyme reactor was developed and used for starch hydrolysis and 76% of starch (30 g/l) was hydrolyzed with 1 hr residence time.