• Title, Summary, Keyword: NF

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The production and characterization of anti-Naegleria fowleri monoclonal antibodies (Naegleyiu fowleri에 대한 단세포군 항체의 생산과 그 특성에 관한 연구)

  • 류재숙;임경일
    • The Korean Journal of Parasitology
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    • v.30 no.1
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    • pp.33-42
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    • 1992
  • Naegleria fowleri, a free-living amoeba commonly found in moist soil and fresh water, enters the body via the nasal mucosa and migrates along the olfactory nerve to t he brain, where it causes acute amoebic meningoencephalitis. In the present study 7 clones secreting monoclonal antibodies (McAbs) against N. fowleri were produced and the effector function of them was investigated. Their isotopes were IgGl (Nf 1, Nf 154), 19G3 (Nf 137) and 19A (Nf 1, Nf 2, Nf 256, Nf 279). Five McAbs (McAb Nf 2, Nf 279, Nf 27, Nf 154, Nf 137) were specific for N. fowleri by ELISA and recognized the antigenic determinants located on the trophoBoite surface by IFAT and immunoperoxidase stain. These aye McAbs had capacity to agglutinate N. fowleri trophozoites and inhibited the growth of the amoeba in culture medium. McAb Nf 2 inhibited proliferation of trophozoites in vitro significantly. Also the cytotoxicity of JV. fowleri against CHO cell was reduced in the presence of McAb Nf 2 and McAb Nf 154. From these results McAb Nf 2 was confirmed to weaken the virulence of the amoeba among 7 screened McAbs.

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Species Dependence of Neurofilament Structures: Monte Carlo Simulation studies of Residue-Based Neurofilament Models

  • Kim, Seon-Ok
    • Proceeding of EDISON Challenge
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    • pp.225-235
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    • 2014
  • 6종의 Intermediate filament 중 type IV인 Neurofilaments (NFs)는 신경세포에 존재하는 세포골격세사로 heavy NF(NF-H), medium NF(NF-M), light NF(NF-L) 세가지의 분자 질량 단백질로 구성되어 있다. NF의 side arm은 interfilament spacing과 axonal caliber를 조절하는 중요한 역할을 한다고 생각되어왔다. 또한 이에 대해서 각각의 protein의 역할은 알아내기 위해 isolated NF의 형태와 구조에 대해 많은 연구가 이루어졌는데, NF의 구조적 특성은 NF sidearm의 tail 부분에서 phosphorylation의 정도에 따른 Lys-Ser-Pro(KSP) repeats의 charge distribution을 통해 알 수 있다. 지금까지 NF에 대한 많은 연구가 이루어졌지만 인간에 한해서만 진행되었다. 그렇기 때문에 본 연구에서는 주어진 amino acid sequence와 각 species의 NF-H:NF-M:NF-L의 비율의 정보를 이용하여 The constant-NVT ensemble MC simulation을 통해 인간뿐만이 아닌 다른 species에 대한 NF의 구조적 특성을 알아보고자 한다.

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Developmental Expression of Neurofilament 3 (NF-M) in the Cultured Rat Cortical Neurons (배양한 흰쥐 대뇌신경세포에서 신경미세섬유 3(NF-M)의 발생학적 표현)

  • Jung Jae-Seob;Cho Sun-Jung;Jin IngNyol;Jung Seung Hyun;Moon Il Soo
    • Journal of Life Science
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    • v.15 no.4
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    • pp.600-606
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    • 2005
  • Neurofilament (NF) proteins constitute the major intermediate filament type in adult neurons. They are made up by the copolymerization of the neurofilament light (NF-L, 61 kDa), medium (NF-M, 90kDa), and heavy (NF-H, 115 kDa) proteins. Although neurofilaments play a crucial .ole in neuronal growth, organization, shape, and plasticity, their expression pattern and cellular distribution in the developing neurons remain unknown. In this study, we have produced a rabbit polyclonal antibody specific to NF-M and investigated expression of NF-M in cultured cortical neurons. Immunostaining of 12 and 24 h cultures revealed strong expression of NF-M in axonal growth cone and in the region of a soma toward the axon. Doublestaining of 4 and 14 DIV corical neurons with NF-M and PSD95 antibodies revealed that both axon and dendrites were stained intensely with NF-M antibody, and that NF-M immunostaining along dendrites is often punctate and colocalize with PSD95 puncta, indicating that the puncta represent postsynaptic spines. Presence of NF-M in the postsynaptic spine was also indicated by immunoblot analysis of the postsynaptic density fraction. Taken together, our results show intensive targeting of NF-M into axons in the early axonal development, and into spines in mature neurons, indicating its important functions in axon and spine development.

A case of neurofibromatosis(NF-I) (신경섬유종증(Neurofibromatosis) 환아(患兒) 1예(例)에 대한 증례보고(症例報告))

  • Min Sang-Yeon;Jang Gyu-Tae;Kim Jang-Hyun
    • The Journal of Pediatrics of Korean Medicine
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    • v.15 no.2
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    • pp.69-73
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    • 2001
  • The neurofibromatosis (NF) are a set of genetic disorders which cause tumors to grow along various types of nerves and, in addition, can affect the development of non-nervous tissues such as bones and skin. NF causes tumors to grow anywhere on or in the body. It also leads to developmental abnormalities. For example, individuals with NF have a higher incidence of learning disabilities. Neurofibromatosis(NF) has been classified into two distinct types: NF-I and NF-II. neurofibromatosis 1(NF-I), also known as von Recklinghausen NF or Peripheral NF, occurring in 1:4,000 births, is characterized by multiple cafe-au-lait spots and neurofibromas on or under the skin. Enlargement and deformation of bones and curvature of the spine (scoliosis) may also occur. Occasionally, tumors may develop in the brain, on cranial nerves, or on the spinal cord. About 50% of people with NF also have learning disabilities. Neurofibromatosis 2(NF-II), also known as Bilateral Acoustic NF(BAN), is much rarer occurring in 1:50,000 births. NF-II is characterized by multiple tumors on the cranial and spinal nerves, and by other lesions of the brain and spinal cord. Tumors affecting both of the auditory nerves are the hallmark. Hearing loss beginning in the teens or early twenties is generally the first symptom. We reported a 10-year-old female patient with NF-I, she has pain and edema in left leg, no symptoms of NF.

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Kinematics of the Northern Filament in Orion Molecular Clouds Complex Using 12CO Molecular Observation Data (12CO 분자선 관측 자료를 이용한 오리온 분자운 복합체내 북쪽 필라멘트의 운동학 연구)

  • Jo, Hoon;Sohn, Jungjoo;Kim, ShinYoung;Lee, JeeWon;Kim, Sungsoo S.;Morris, Mark
    • Journal of the Korean earth science society
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    • v.39 no.6
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    • pp.519-532
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    • 2018
  • We investigated the effect of galactic plane toward molecular motion and kinematics in the northern filament (NF) of Orion Molecular Clouds Complex (OMC) using $^{12}CO$ (J=1-0) line. Observed data were from three areas including NF1, NF2, and NF3 in far-out order from galactic plane, for a total 270 hours by Seoul National University Radio Astronomy Observatory (SRAO) 6m telescope, with 2arcmin spatial resolution. galactic plane and OMC NF were connected to each other along the magnetic field at a density of 3% for $^{12}CO$ (J=2-1) and 9% for the case of dust. $^{12}CO$ (J=1-0), $^{12}CO$ (J=2-1), and interstellar dusts were distributed uniformly in NF3, but only in certain regions with relatively high density in NF1 and NF2. NF showed a single structure, partial shrinking motion in NF1, and rotational motion at the bottom of NF2, and spiral rotation associated with magnetic field only in NF3. The position-velocity analysis showed that the materials including $^{12}CO$ (J=1-0) could flow toward galactic plane along NF2 and NF3. However, there was no clear cause for the material to flow toward galactic plane in this result. Further detailed observation for rotational motion at the top of NF1 and NF2 might help to confirm it.

The effect of rod domain A148V mutation of neurofilament light chain on filament formation

  • Lee, In-Bum;Kim, Sung-Kuk;Chung, Sang-Hee;Kim, Ho;Kwon, Taeg-Kyu;Min, Do-Sik;Chang, Jong-Soo
    • BMB Reports
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    • v.41 no.12
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    • pp.868-874
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    • 2008
  • Neurofilaments (NFs) are neuronal intermediate filaments composed of light (NF-L), middle (NF-M), and heavy (NF-H) subunits. NF-L self-assembles into a "core" filament with which NF-M or NF-H co-assembles to form the neuronal intermediate filament. Recent reports show that point mutations of the NF-L gene result in Charcot-Marie-Tooth disease (CMT). However, the most recently described rod domain mutant of human NF-L (A148V) has not been characterized in cellular level. We cloned human NF-L and used it to engineer the A148V. In phenotypic analysis using SW13 cells, A148V mutation completely abolished filament formation despite of presence of NF-M. Moreover, A148V mutation reduced the levels of in vitro self-assembly using GST-NF-L (H/R) fusion protein whereas control (A296T) mutant did not affect the filament formation. These results suggest that alanine at position 148 is essentially required for NF-L self-assembly leading to subsequent filament formation in neuronal cells.

Phenotypic Analysis of Neurofilament Light Chain E397K Mutant in Cultured Cells

  • Kim, Sung-Kuk;Chang, Jong-Soo
    • Biomedical Science Letters
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    • v.12 no.4
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    • pp.413-418
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    • 2006
  • Charcot-Marie-Tooth disease (CMT) is blown as one of the inherited disorder of peripheral nervous system. Recently, it was found that point mutations in the neurofilament light subunit (NF-L) gene cause CMT. Neurofilaments (NFs) are heteropolymers consist of NF-L, NF-M and NF-H. To assess the relationship between CMT and NF-L mutation in cellular level, we performed phenotypic analysis of the mutant NF-L (E397K) using cultured cell lines. Vimentin-deficient human adrenal carcinoma SW13 (Vim-) cells have a potential to form the intermediate filaments when the cells are expressing both NF-L and NF-M. Our results show that co-expression of wild type NF-L with NF-M showed intermediate filament formation in SW13 (Vim-) cells, while E397K with NF-M did not. This result means that E397K mutant lost its ability to form the intermediate filament in vivo, and further suggests that the E397K mutation is closely related to CMT.

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NF-Y binds to both G1- and G2-specific cyclin promoters; a possible role in linking CDK2/Cyclin A to CDK1/Cyclin B

  • Chae, Hee-Don;Kim, Jung-Bin;Shin, Deug-Y.
    • BMB Reports
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    • v.44 no.8
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    • pp.553-557
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    • 2011
  • We previously reported that CDK2/Cyclin A can phosphorylate and activate the transcription factor NF-Y. In this study, we investigated a potential regulatory role for NF-Y in the transcription of Cyclin A and other cell cycle regulatory genes. Gel-shift assays demonstrate that NF-Y binds to CCAAT sequences in the Cyclin A promoter, as well as to those in the promoters of cell cycle G2 regulators such as CDC2, Cyclin B and CDC25C. Furthermore, expression of Cyclin A increases NF-Y's affinity for CCAAT sequences in the CDC2 promoter; however, Cyclin A's induction of CDC2 transcription is antagonized by p21, an inhibitor of CDK2/Cyclin A. These results suggest a model wherein NF-Y binds to and activates transcription from the Cyclin A promoter, increasing cellular levels of Cyclin A/CDK2 and potentiating NF-Y's capacity for transcriptional transactivation, and imply a positive feedback loop between NF-Y and Cyclin A/CDK2. Our findings are additionally indicative of a role for Cyclin A in activating Cyclin B/CDK1 through promoting NF-Y dependent transcription of Cyclin B and CDC2; NF-Y mediated crosstalk may therefore help to orchestrate cell-cycle progression.

The Effect of Cobrotoxin on $NF-{\kappa}B$ binding Activity in Raw264.7 cells

  • Yoo, Jae-Ryong;Song, Ho-Sueb
    • Journal of Acupuncture Research
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    • v.22 no.2
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    • pp.133-139
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    • 2005
  • Cobrotoxin, a venom of Vipera lebetina turanica, is a group of basic peptidescomposed of 233 amino acids with six disulfide bonds formed by twelve cysteins. NF-kB is activated by subsequent release of inhibitory IkB and translocation of p50. Since sulfhydryl group is present in kinase domain of p50 subunit of NF-kB, cobrotoxin could modify NF-kB activity by protein-protein interaction. We therefore examined effect of cobrotoxin on NF-kB activities in lipopolysaccharide (LPS) and sodium nitroprusside (SNP)-stimulated Raw 264.7 mouse macrophages. Cobrotoxin suppressed the LPS and SNP-induced release of IkB and p50 translocation resulted in inhibition of DNA binding activity of NF-kB. Inhibition of NF-kB resulted in reduction of the LPS and SNP-induced production of inflammatory mediators NO and PGE2 generation. The inhibitory effect of cobrotoxin on the NF-kB activity were blocked by addition of reducing agents dithiothreitol and glutathione. These results demonstrate that cobrotoxin inhibits activation of NF-kB, and suggest that pico to nanomolar range of cobrotoxin could inhibit the expression of genes in the NF-kB signal pathway.

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Kinematic Study of Northern Filament in Orion Molecular Clouds Complex By 12CO Radio Observation

  • Jo, Hoon;Sohn, Jungjoo;Kim, ShinYoung;Lee, Jee Won;Kim, Sungsoo;Morris, Mark
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.2
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    • pp.52.1-52.1
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    • 2018
  • Orion Molecular Clouds Complex(OMC) 분자운에는 별 생성은 없으면서 은하면 방향으로 누워있는 큰 규모($10^{\circ}{\times}0.5^{\circ}$)의 필라멘트 구조가 있다. 본 연구는 북쪽 필라멘트(이하 NF)를 대상으로 12CO (J = 1-0) 선 관측 데이터를 이용하여 필라멘트의 운동학적 연구를 수행함으로서 은하면과의 상관관계를 알아보고자 하였다. 관측은 공간분해능은 2 arcmin인 SRAO(Seoul Radio Astronomy Observatory)의 6m 밀리미터 망원경이 사용되었고 큰 규모로 인해 은하면으로부터 먼 순서로 NF1, NF2, NF3 세 곳으로 관측 지역이 정해졌다. 연구결과 필라멘트는 매우 낮은 수준의 12CO (J = 2-1)과 티끌 분포에서 자기장을 따라 은하면 방향으로 연계되어 보였다. 밀도 분포에서는 SRAO 12CO (J = 1-0) 적분강도와 Planck 위성의 12CO (J = 2-1)과 티끌 자료를 이용했을 때, 12CO와 성간 티끌은 주로 은하면에 수직인 방향에서 밀도가 높았다. 속도 분포와 위치 속도 분석을 통해 NF는 단일 구조의 분자운 형태이고 NF2 하단에서는 회전 운동의 가능성이 확인되었다. NF3는 자기장에 의해 생성된 나선형 회전을 하고 있으며, NF2와 NF3를 따라 은하면을 향하여 12CO (J = 1-0)를 비롯한 물질이 흐르고 있음도 확인되었다. 하지만 은하면을 향하여 물질이 흐르는 원인을 제공하는 천체가 무엇인지와 NF1과 NF2 상단의 회전 운동은 확인 할 수 없었으며 이들 지역에 대한 상세한 관측이 요구된다.

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