• Title, Summary, Keyword: Molasses

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Feeding Value of Urea Treated Wheat Straw Ensiled with or without Acidified Molasses in Nili-Ravi Buffaloes

  • Khan, M. Ajmal;Sarwar, Muhammad;Nisa, M.;Khan, M.S.;Bhatti, S.A.;Iqbal, Z.;Lee, W.S.;Lee, H.J.;Kim, H.S.;Ki, K.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.5
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    • pp.645-650
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    • 2006
  • Thirty early lactating Nili-Ravi buffaloes, six animals in each group, were used in a completely randomized design to examine the feeding value of 4% urea treated wheat straw (UTWS) ensiled with 6% or without acidified molasses. Five experimental diets were formulated. The control ration was balanced to contain 30% DM from UTWS ensiled without acidified molasses. The other four diets were formulated to have 30, 40, 50 and 60% DM from UTWS ensiled with 6% acidified molasses, respectively. Dry matter and neutral detergent fiber (NDF) intakes were higher in buffaloes fed diets containing UTWS ensiled with acidified molasses compared with those fed a diet containing UTWS ensiled without acidified molasses. Intake of DM was not significantly different in buffaloes fed diets containing varying levels of UTWS ensiled with acidified molasses. A similar trend was observed for crude protein (CP) intake. Apparent DM and NDF digestibilities were significantly higher in buffaloes fed diets containing UTWS ensiled with acidified molasses compared with those fed UTWS ensiled without acidified molasses. However, differences in DM and NDF digestibilities were non-significant across buffaloes fed diets containing varying levels of UTWS ensiled with acidified molasses. Milk yield (4% fat corrected) was significantly higher in buffaloes fed diets containing UTWS ensiled with acidified molasses than those fed a diet containing UTWS ensiled without acidified molasses. Milk yield was similar in buffaloes fed varying level of UTWS ensiled with acidified molasses. Milk CP, true protein, solid-not-fat and total solids were similar in buffaloes fed UTWS ensiled with or without acidified molasses. The UTWS ensiled with 6% acidified molasses can be included at up to 60% DM of lactating buffalo rations without any ill effect on productivity.

Molasses clarifying method used by lime-phosphate for yeast culture (효모배지용 당밀의 청징법에 관하여)

  • 임억규
    • Korean Journal of Microbiology
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    • v.19 no.3
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    • pp.121-127
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    • 1981
  • The constituents of molasses and effect of pH precipitate formation in molasses solution, vary according to its producing districts. The formation of precipitation is not so changeable in the range of buffering zone of molasses solution(pH4.3-6.3) in philippine molasses according to the change of pH value. On lower or higher than the range of buffering zone, the precipitation is increased from pH 4.3 to 2.8 and from 6.3 to 8.1, it is decreased when pH value is lower or higher than the pH value range. For molasses clarifying, it had better adjust the pH of molasses solution to neutral or weak alkali range out of the alkai side of the buffering zone, with lime solution. And then, add the calcium super phosphate solution to pH value of alkali side in buffering zone, as much as the pH of clarified molasses solution can reach to middle value in buffering zone. For the equilibrium of pH value on clarifying molasses, it takes plenty of time more than 6 hours.

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Production of Dye-decolorizing Enzyme using Molasses-containing Medium

  • Lee, Tae-Ho;Shoda, Makoto
    • 한국생물공학회:학술대회논문집
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    • pp.57-58
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    • 2000
  • Production of dye-decolorizing enzyme was investigated with the cultivation of Geotrichum candidum Dec1 (abbreviated to Dec1) using molasses as a cheap raw materials. Molasses was found to stimulate the enzyme production as well as a role of carbon source, because the production increased with molasses content up to 50 g/L. However, the severe inhibition of dye-decolorizing enzyme activity was observed even at low concentration of molasses 10 g/L when purified decolorizing peroxidase was used. Its inhibitory effect was reduced through the cultivation of Dec1. The fractions of molasses separated by a gel chromatography showed the different degrees of inhibition. As a way to reduce the inhibitory effect, the dilution of culture broth was examined, and the total decolorizing activity for Reactive blue 5 increased 7 times as much as that of original culture broth by 30 times dilution. On the basis of result, we proposed a process scheme which can fully utilize both positive and negative effect of molasses in dye-decolorizing process using molasses.

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Effects of Adding Urea and Molasses on Napiergrass Silage Quality

  • Yunus, M.;Ohba, N.;Shimojo, M.;Furuse, M.;Masuda, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.11
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    • pp.1542-1547
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    • 2000
  • To standardize proper formulation of urea and molasses, the former to increase crude protein content of tropical grass and the latter for improving its silage quality, we examined the fermentation quality of silage of fresh and wilted napiergrass (Pennisetum purpureum Schumach) with different levels of urea and molasses with or without lactic acid bacteria (LAB). Silage was made of napiergrass with conditions of fresh young (Exp. 1),young wilted for half day (Exp. 2) and fresh mature (Exp. 3). Chopped plant materials of about 1cm length were ensiled into a laboratory silo and incubated for one month at $25^{\circ}C$. The treatments were the combination of 0, 0.2 and 0.6% of urea and 0, 2 and 5% of molasses (fresh material basis) with or without LAB inoculation. After opening the silo, pH, organic acids, volatile basic nitrogen (VBN) and total nitrogen (TN) were determined. Addition of molasses significantly (p<0.01) lowered pH values in three experiments. Though molasses addition increased lactic acid production even at a higher level of urea, pH values at 0 and 2% molasses were significantly increased by urea in fresh and wilted young silages, but in fresh mature silage it occurred only when molasses was not added. VBN/TN at 0.6% urea were decreased significantly by the highest molasses in three experiments. Significant increases in TN by the increasing of urea addition were observed at all levels of molasses in wilted young and fresh mature silages. In conclusion, a combination of 5% molasses and 0.6% urea could improve the nutritive and fermentation qualities of napiergrass silage under young, wilting and mature conditions.

Effects of Dietary Microbial-Fermented Molasses on Egg Production and Egg Quality in Laying Hens (미생물 발효 당밀을 산란계 사료에 첨가 시 계란생산성과 특성에 미치는 영향에 관한 연구)

  • Choi, In Hag
    • Journal of Environmental Science International
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    • v.28 no.1
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    • pp.159-162
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    • 2019
  • This study aimed to evaluate the effects of dietary microbial-fermented molasses on egg production and egg quality in laying hens.In total, 90 Hy-line Brown laying hens were divided into two treatment groups (control and 1% microbial-fermented molasses)with three replicates of 15 birds each. During the experimental period, supplementation of hen diets with 1% microbial-fermented molassesdid not influence egg weight, hen-day egg production, egg mass, and feed conversion ratio (p > 0.05), except for feed intake. Regarding egg quality, diets containing 1% microbial-fermented molasses significantly affected eggshell thickness, Haugh unit, and albumen height (p < 0.05). However, there were no remarkable differences between control and 1% microbial-fermented molasses in eggshell color and egg yolk color (p > 0.05). These results indicate that supplementing 1% microbial-fermented molasses to the diet of laying hens improved egg quality parameters such as eggshell thickness, Haugh unit, and albumen height rather than egg production.

Stimulatory Effects of Sugarcane Molasses on Fumigaclavine C Biosynthesis by Aspergillus fumigatus CY018 via Biofilm Enhancement

  • Tao, Jun;An, Fa-Liang;Pan, Zheng-Hua;Lu, Yan-Hua
    • Journal of Microbiology and Biotechnology
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    • v.28 no.5
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    • pp.748-756
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    • 2018
  • Biofilms are of vital significance in bioconversion and biotechnological processes. In this work, sugarcane molasses was used to enhance biofilms for the improvement of the production of fumigaclavine C (FC), a conidiation-associated ergot alkaloid with strong anti-inflammatory activities. Biofilm formation was more greatly induced by the addition of molasses than the addition of other reported biofilm inducers. With the optimal molasses concentration (400 g/l), the biofilm biomass was 6-fold higher than that with sucrose, and FC and conidia production was increased by 5.8- and 3.1-fold, respectively. Moreover, the global secondary metabolism regulatory gene laeA, FC biosynthetic gene fgaOx3, and asexual central regulatory genes brlA and wetA were upregulated in molasses-based biofilms, suggesting the upregulation of both asexual development and FC biosynthesis. This study provides novel insight into the stimulatory effects of molasses on biofilm formation and supports the widespread application of molasses as an inexpensive raw material and effective inducer for biofilm production.

Effect of Molasses Addition Level to the Mixture of Calf Starter and Corn Fodder on Pellet Quality, Rumen Development and Performance of Holstein-Friesian Calves in Indonesia

  • Mukodiningsih, Sri;Budhi, S.P.S.;Agus, A.;Haryadi, Haryadi;Ohh, Sang-Jip
    • Journal of Animal Science and Technology
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    • v.52 no.3
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    • pp.229-236
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    • 2010
  • Effect of molasses addition to complete calf starter (CCS) and the following pellet processing was evaluated with 12 Holstein-Friesian (HF) calves (7-14 d old with $42\;{\pm}\;5.5\;kg$ average BW), with 6 calves (replicate) per treatment. The CCS was formulated with 65% concentrate calf starter and 35% local corn fodder. On the CCS, molasses was added either 5% (M5) or 10% (M10), then the mixture was pelleted and fed to the calves for 7 weeks. Molasses addition, regardless of addition level, improved (p<0.05) both the durability and hardness of the pellet which contains 35% of high fibrous local corn fodder. Upon feeding to calves, the feed intake and daily gain were numerically higher with 5% molasses addition compared to 10% molasses addition. Blood VFA level was remarkably higher (p<0.01) in calves fed M10 than calves fed M5. There was no difference (p>0.05) in blood glucose level between M5 and M10. Length and number of papillae were not different (p>0.05) by the addition levels of molasses. However, there was one exception in number of papillae at caudo-dorsal sac which were higher (p<0.05) number in M5 than M10 calves. Considering the quality of CCS pellet, calf performance and rumen development, there was no additional benefit of 10% molasses addition compared to 5%. From the results of this study, 5% molasses addition could be recommended for local farmer when they utilize local corn fodder to make CCS for HF calves in Indonesia.

Cassava Tops Ensiled With or Without Molasses as Additive Effects on Quality, Feed Intake and Digestibility by Heifers

  • Van Man, Ngo;Wiktorsson, Hans
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.5
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    • pp.624-630
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    • 2001
  • Two experiments on the effects of molasses additive on cassava tops silage quality to its feed intake and digestibility by growing Holstein$\times$local crossbred heifers were carried out. Sixteen plastic bags of one meter diameter and two meters length were allocated in a $2{\times}2$ factorial design with four replicates in the ensiling study, with and without the molasses additive and with two storage times (2 and 3 months). The silage produced in the first experiment was used in the feed intake and digestibility study. Six crossbred Holstein heifers, 160-180 kg live weight, were randomly allocated in a $3{\times}2$ change-over design to three treatments: Guinea grass ad libitum, 70% of grass ad libitum with a supplement of non-molasses cassava silage ad libitum, and 70% of grass ad libitum with a supplement of molasses cassava silage ad libitum. Ensiling was shown to be a satisfactory method for preservation of cassava tops. The HCN content was significantly reduced from $840mg\;kg^{-1}$ to 300 or $130mg\;kg^{-1}$, depending on storage period. The tannin content was not significantly changed. Molasses additive resulted in lower pH, Crude Protein (CP), NDF and higher DM content but did not otherwise affect chemical composition. The voluntary feed intake per 100 kg live weight of the heifers was 2.59, 2.65 and 2.91 kg DM of Guinea grass, non-molasses cassava tops silage and molasses cassava tops silage diet, respectively. Crude protein intake was significantly improved in the cassava tops silage diets. The apparent digestibility of DM, OM, CP, NDF and ADF decreased with the silage supplement diets. No significant difference in digestibility was found between the non-molasses and molasses silage diets. The digestibility coefficient of DM, OM, CP, NDF, ADF in non-molasses cassava tops silage and molasses cassava tops silage was 49.4, 52.1, 45.81, 36.6, 27.7 and 49.7, 51.9, 47.55, 28.1, 19.5, respectively. It is concluded that cassava tops can be preserved successfully by ensiling and that cassava tops silage is a good feed resource for cattle.

ESTIMATION OF SUGAR AND REDUCING SUGAR IN MOLASSES USING NEAR INFRARED REFLECTANCE SPECTROSCOPY

  • Mehrotra, Ranjana;Gupta, Alka;Tewari, Jagdish;Varma, S.P.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • pp.1258-1258
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    • 2001
  • Estimation of sugar and reducing sugar content in molasses is very important task in sugar refineries. Conventional methods of determination of sugar content in molasses samples are highly time consuming and employ hazardous chemicals. Due to the physical properties of molasses, probability of error in conventional analytical techniques is high. These methods have proven to be inefficient for a process control in any sugar industry. Hence development of a rapid, inexpensive, physical and also accurate method for sugar determination in molasses will be highly useful. Near Infrared spectroscopy is being widely used worldwide as an analytical technique in food industry. The technique offers the advantage of being non-destructive and rapid. The present paper highlights the potential of near infrared reflectance spectroscopy as a rapid and automated analytical technique for determination of sugar and reducing sugar content in molasses. A number of molasses samples were collected during and after the sugar season from Havana Sugar Industry, Havana. The samples were chosen so as to obtain a wide range of concentration of sugar and reducing sugars. This was done in order to achieve a good calibration curve with widely spread data points. These samples were scanned in the region of 1100 - 2500 nm in diffuse reflectance mode. An indigenous ELICO NIR spectrophotometer, modified according to the requirements of sugar industry was used for this purpose. Each sample was also analyzed simultaneously by standard chemical methods. Chemical values were taken as reference for near infrared analysis. In order to obtain the most accurate calibration for the set of samples, various mathematical treatments were employed. Partial Least Square method was found to be most suitable for the analysis. A comparison is made between the actual values (chemical values) and the predicted values (NIR values). The actual values agree very well with the predicted values showing the accuracy of the technique. The validity of the technique is checked by predicting the concentration of sugar in unknown molasses samples using the calibration curve. The present investigation assesses the feasibility of the technique for on-line monitoring of sugars present in molasses in sugar industries.

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Constitutive Expression of Bacillus stearothermophilus CGTase in Bacillus subtilis. (Bacillus subtilis에서 Bacillus stearothermophilus CGTase의 구성적 발현)

  • 허선연;김중균;권현주;김병우;김동은;남수완
    • Journal of Life Science
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    • v.14 no.3
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    • pp.391-395
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    • 2004
  • To overproduce the cyclodextrin glucanotransferase(CGTase) of Bacillus stearothermophilus NO2 in B. subtilis, the pJH-CGTl plasmid (8.14 kb) was constructed, in which the ORF of CGTase gene could be transcribed by strong constitutive promoter, P$\_$JH/. To overproduce CGTase from a recombinant B. subtilis, the effect of media on the cell growth and expression level of CGTase were investigated with various media (LB, 2${\times}$LB, 5% molasses+2% CSL, CS, LBG) in the flask culture. Among them, [5% molasses+2% CSL] medium revealed the maximum expression level of CGTase with 1.8 unit/$m\ell$ at 9 hr culture. In the batch culture on [10% molasses+5% corn steep liquor] medium the expression level of CGTase, the secretion efficiency, and plasmid stability were about 4.2 unit/$m\ell$, 90% and 90%, respectively, at 30 hr culture. The cell growth and expression level in the fermenter culture with the industrial molasses medium were increased by 2-folds over the flask culture.