• Title, Summary, Keyword: Microwave microscopy

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Fixation of Cellular Ultrastructure by the Microwave Irradiation (마이크로파 조사에 의한 세포 미세구조의 고정 효과)

  • Shin, Kil-Sang;Kim, Wan-Jong;Jeon, Jin-Seok
    • Applied Microscopy
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    • v.26 no.4
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    • pp.401-410
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    • 1996
  • The microwave fixator has recently been introduced in morphological research. The present study was carried out to investigate the ultrastructural effects of microwave fixation of rat brain. kidney, liver and skeletal muscle tissues. The results are as follows: In the case of microwave fixed cerebrum. the cytoplasmic processes of neurons and the various membranous organelles such as nuclear envelope, mitochondria, rough endoplasmic reticulum and Golgi apparatus were well preserved, The myelin sheath wrapping neuronal axon was prominent. Microwave fixed hepatocytes showed the microvilli on the free surface of bile canaliculus, the evident nucleolar components, and typical organelles. In nephron, ultrastructures of glomerulus and Bowman's capsule were preserved, and also tubular wall were structurally observed. Among the skeletal muscle cells, plentiful collagen fibers were appeared, myofibrils and mitochondria were typically observed. In conclusion, the microwave fixation procedures result in an good preservation of the tissues and would be time- and reagent-saving.

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Microwave fixation of Setaria Digitata for scanning electron microscopy (선충류의 주사전자현미경적 관찰을 위한 마이크로웨이브 고정법)

  • Lee, San-Soo;Cho, Kyoung-Oh;Shin, Kil-Sang;Shin, Sung-Shik
    • Korean Journal of Veterinary Research
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    • v.47 no.2
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    • pp.203-207
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    • 2007
  • Conventional processing of biological materials including nematode parasites for scanning electron microscopy includes fixation with glutaraldehyde and osmium, followed by dehydration in an ascending grade of ethanol, and finally freeze drying. This procedure takes about 8 to 12 h depending on the characteristics of samples. Microwave irradiation of 2,450 MHz enhance the action of cross-linking fixatives and can greatly accelerate various stages of tissue processing. In this study, samples of nematode parasites, Setaria digitata, were fixed by a combination of conventional chemical fixation and the microwave irradiation during the process. The microwave irradiation was also incorporated in the serial dehydration process with ethanol. The complete procedure from the initial fixation to the completion of dehydration with ethanol was reduced to 1 h with good preservation of the ultrastructural details of the specimens.

Control of Particle Characteristics in the Preparation of TiO2 Nano Particles Assisted by Microwave

  • Lee, Han-Bin;Choi, Min-Sik;Kye, Youn-Hee;An, Myoung-Young;Lee, Ik-Mo
    • Bulletin of the Korean Chemical Society
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    • v.33 no.5
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    • pp.1699-1702
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    • 2012
  • $TiO_2$ nanostructures with various morphologies like cubes, spheres, hexahedral pillars and spherical tubes were synthesized by microwave-assisted hydrothermal process. Each structure was obtained by changing the relative concentrations of titanium tetraisoproxide (TTIP), tetrabutylammonium hydroxide (TBAH) and ethanol. Scanning electron microscopy (SEM), transmission electoron microscopy (TEM), X-ray diffraction and Brunauer-Emmett-Teller (BET) surface area analysis were used to characterize the synthesized $TiO_2$ nanostructures. From these results, it has been proved that $TiO_2$ structure could be controlled to have specific morphology, size, surface area, pore volume and pore size distribution.

Fixation and Histochemistry of Biological Tissues Using the Microwave Fixator Equipped with Infrared-Temperature Sensor (적외선 온도감응기를 장착한 마이크로파 고정기에 의한 생체조직 고정효과와 조직화학적 특성)

  • 신길상;민소연;김완종;손태호
    • The Korean Journal of Zoology
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    • v.38 no.3
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    • pp.417-425
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    • 1995
  • The present study was carried out to investigate the effect of microwave fixation in comparison with that of chemical fixation in preparing the microscopic samples. The microwave fixator was equipped with infrared-temperature sensor, and that was designed to compensate air temperature in the microwave fixator. In the microwave fixation, rat tongue was well preserved in terms of muscular fasciculus and pancreas stained by Feulgen reagents showed clear reaction products in the nucleus. Reaction products by PAS method in duodenal villi appeared specifically at the goblet cells. In electron microscopy, pancreatic cellular components such as secretory granules and collagen bundles were well preserved in both fixations. In aspect of histochemical reaction and electron microscopy, high quality was due to the protein content of microwave fixed specimen. The microwave fixation method saved total duration engaging microscopic preparation.

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The Fixation Effects in Immunohistochemistry and Electron Microscopy Using Low Energy of Microwave (LEM) in Human Gastric Adenocarcinoma and HeLa Cell (사람 위선암과 HeLa 세포에 관한 저에너지 마이크로파 고정효과의 조직화학 및 전자현미경적 연구)

  • Yang, Seung-Ha;Son, Tae-Ho;Shin, Kil-Sang
    • Applied Microscopy
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    • v.31 no.2
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    • pp.185-197
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    • 2001
  • Human gastric adenocarcinomas are fixated with low energy of microwave (LEM) to study fixation effects in level of ultrastructure and antigenicity of the cancer. For the Ag-Ab reactions , the LEM fixated sdenocarcinomas are incorporated with monoclonal mouse anti-human p53 (IgG2b, kappa) and rabbit anti human cerbB-2. The retrieval of antigenicity are easily recognizable in the LEM fixated sections compared with that of frozen sections which show often diffused colour reactions. And the LEM fixation methods have preserved ultrastructures of the adenocarcinoma, but it was often difficult to maintain constancy in fixation effects. For the constancy, LEM was coupled with low concentration of chemical fixatives, such as glutaraldehyde (<1%) and $OsO_4$ (<0.5%). The results were acceptable, but there are tendencies that the adenocarcinoma requisitioned rather weak microwave energy to come into the optimal fixation effects. Therefore , cultured HeLa cells were fixated with lower energy of microwave than that used to the adenocarcinoma. The ultrastructures of the single HeLa cell have been preserved. The results may imply that a different energy levels of microwave are requisitioned in accordance with kinds of cells and tissues for the optimal fixation effects. It is reported and discussed that the fixation methods of LEM used in this work could be applied routinely to conceal a insufficient diffusion rate of chemical fixatives into some kinds of cancer without compromising the ultrastructures as well as to improve antigenic quality of frozen sections.

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Preparation and Characterization of Surface Modified Mica by Microwave-enhanced Wet Etching (마이크로웨이브로 증폭된 습식 에칭에 의한 표면 개질 마이카의 제조와 특성)

  • Jeon, Sang-Hoon;Kwon, Sun-Sang;Kim, Duck-Hee;Shim, Min-Kyung;Choi, Young-Jin;Han, Sang-Hoon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.34 no.4
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    • pp.269-274
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    • 2008
  • In this study we successfully altered the structural characteristics of the mica surface and were able to control oil-absorption by using the microwave enhanced etching (MEE) technique, which has originally been used in semiconductor industry. When microwave energy is applied to the mica, the surface of the mica is etched in a few minutes. As the result of etching, oil-absorption of the mica was enhanced and surface whiteness was improved by modifying the silicon dioxide layer. Additionally, the high whiteness was maintained even though the etched mica absorbed the sebum or sweat. The surface modification of mica was performed by microwave irradiation after the treatment of hydrofluoric acid. The degree of etching was regulated by acid concentration, irradiation time, the amount of energy and slurry concentration. The surface morphology of the etched mica appears to be the shape of the 'Moon'. The characteristics of surface area and roughness were examined by Brunauer-Emmett-Teller (BET) surface area analysis, atomic force microscopy (AFM), scanning electron microscopy (SEM), spectrophotometer and goniophotometer.

The Synthesis of Crystalline-Size Controlled Organic Pigment by Microwave Energy (마이크로파 에너지에 의해 결정 크기가 조절된 유기 안료의 합성)

  • 정기석;고진필;박상보;박찬영;민성기;권종호;오인환;박성수
    • Polymer Korea
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    • v.25 no.5
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    • pp.609-616
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    • 2001
  • The application of microwave technology to synthesis of polymer in solvent media has been shown by the synthesis of copper phthalocyanine (CUPc). The increase of synthetic yield-was demonstrated successfully in this study. A power variable microwave synthetic system has been developed with modifying cavity of domestic microwave oven and attaching microwave power controller. The properties of the specimen synthesized at various condition under the conventional thermal processing and microwave processing has been characterized by the means of chemical analysis, X-ray diffractometry (XRD), scanning electron microscopy (SEM), and particle size analysis (PSA).

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Synthesisand Electrochemical Behaviors of Hybrid Carbon (ACF/Graphene) as Supports by Microwaves-irradiation Method for Polymer Exchange Membrane Fuel Cells (PEMFC) (마이크로웨이브를 이용한 고분자 전해질 연료전지용 복합 탄소 촉매 지지체 (ACF/Graphene)의 합성과 전기화학적 거동)

  • Cho, Yongil;Jeon, Yukwon;Park, Dae-Hwan;Juon, So-Me;Kim, Tae-Eon;Oh, Kyeongseok;Shul, Yong-Gun
    • Transactions of the Korean hydrogen and new energy society
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    • v.24 no.2
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    • pp.142-149
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    • 2013
  • Carbon materials are mainly used as catalyst supports for polymer exchange membrane fuel cell (PEMFC). Catalyst supports are required specific characteristics of the carbon materials, such as large surface area and high electrical conductivity. Attempted were to improve electrical conductivity and to maintain high surface area of carbon materials using a microwave treatment. Microwave treatment, as a relatively new technique, takes short reaction time and reduce the consumption of the gases used for carbon treatment compared to a traditional heat treatment. Hybrid carbon (ACF/Graphene) as catalyst supports by microwave-irradiation method for PEMFC increase the cell performance because of increased electrical conductivity resulting in triple-phase contact and reduced the interfacial resistance. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-Ray Diffraction (XRD) were employed to analyze carbon materials. The performance of microwave-treated carbon materials was evaluated by measuring current-voltage (I-V) characteristics and electrode impedance.