In this paper, we proposed a method to shift the resonant frequency by applying the electric field to microstrip patch antenna using piezoelectric substrates. We fabricated microstrip patch antenna using Y-cut LiNbO3, Quartz and FR-4 substrates. We designed and simulated the microstrip antennas by Ensemble V 7.0 of the simulation too1. We observed the resonant frequency by DC applied electric field in a microstrip patch antenna. When the electric field was 300 V/mm, the resonant frequency agile of Y-cut LiNbO3 microstrip patch antennas were 29 MHz. When the electric field was 400 V/mm, the frequency agile of X-cut, Y-cut and Z-cut quartz microstrip patch antennas were 55.2 MHz, 34.2 MHz and 28.0 MHz, respectively. However, when the electric field was 400 V/mm, the resonant frequency of FR-4 microstrip patch antenna does not changed. It was shown that the resonant frequency agile of Y-cut and Z-cut quartz microstrip patch antennas are due to piezoelectric phenomenon not to be permittivity.