• Title, Summary, Keyword: Microsatellite DNA

Search Result 233, Processing Time 0.043 seconds

A Major DNA Marker Mining of BM4311 Microsatellite Loci in Hanwoo Chromosome 6

  • Lee, Jea-Young;Kim, Mun-Jung;Lee, Yong-Won
    • Journal of the Korean Data and Information Science Society
    • /
    • v.14 no.4
    • /
    • pp.759-772
    • /
    • 2003
  • K-Means and Web mining modelling have been tried for finding major DNA marker of BM4311 microsatellite loci in Hanwoo Chromosome 6 linkage map. Furthermore, a major DNA mining by bootstrap simulations(BCa) has been applied.

  • PDF

A Major DNA marker Mining of ILST035 microsatellite loci in Hanwoo Chromosome 6

  • Lee, Jea-Young;Yeo, Jung-Sou;Kim, Jae-Woo;Lee, Yong-Won
    • Journal of the Korean Data and Information Science Society
    • /
    • v.13 no.2
    • /
    • pp.97-104
    • /
    • 2002
  • K-Means modelling has been tried for finding major DNA marker of ILST035 microsatellite loci in Hanwoo Chromosome 6 linkage map. Major DNA markers are obtained from the ILST035 microsatellite through quantitative trait loci(QTL) and data mining modelling.

  • PDF

Construction of a DNA Profile Database for Commercial Cucumber (Cucumis sativus L.) Cultivars Using Microsatellite Marker (Microsatellite 마커를 이용한 오이 유통품종 DNA Profile Data Base 구축)

  • Kwon, Yong-Sham;Choi, Keun-Jin
    • Horticultural Science & Technology
    • /
    • v.31 no.3
    • /
    • pp.344-351
    • /
    • 2013
  • Microsatellite is one of the most suitable marker for cultivar identification as it has great discrimination power for cultivars with narrow genetic variation. The polymorphism level between 358 microsatellite primer pairs and 11 commercial cucumber cultivars was investigated. Thirty-one primer pairs showed high polymorphism within cucumber cultivars with different fruit types. These markers were applied for the constructing DNA profile data base of 110 commercial cucumber cultivars through multiplex PCR and fluorescence based automatic detection system. A total of 139 polymorphic amplified fragments were obtained by using 31 microsatellite markers. The average of PIC value was 0.610 ranging from 0.253 to 0.873. One hundred and thirty nine microsatellite loci were used to calculate Jaccard's distance coefficients for UPGMA cluster analysis. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into plant shape and fruit type. Almost the cultivars were discriminated by marker genotypes. This information may be useful to compare through genetic relationship analysis between existing variety and candidate varieties in distinctive tests and protection of plant breeders' intellectual property rights through variety identification.

DNA fingerprinting analysis of maize varieties and parental lines using microsatellite markers (Microsatellite 마커를 이용한 옥수수 품종 및 자식 계통에 대한 DNA Fingerprinting 분석)

  • Kwon, Yong-Sham
    • Journal of Plant Biotechnology
    • /
    • v.43 no.3
    • /
    • pp.367-375
    • /
    • 2016
  • In the present study, we conducted genetic characterization of 90 commercial maize varieties and parental lines using microsatellite markers. Thirteen microsatellite markers were selected from 100 primer pairs in the maize genome data on the basis of polymorphism information contents (PIC) value and distinct amplification products. These markers detected 5 to 24 alleles, with an average of 13.69. The mean PIC value was 0.865 and ranged from 0.716 to 0.942. The unweighted pair-group method with arithmetical average (UPGMA) analysis was conducted for constructing the dendrogram using Jaccard's genetic similarity coefficient. The genetic similarity varied from 0.07 to 0.824. Thirteen microsatellite markers identified all 90 maize varieties and parental lines. The maize varieties were clustered into 5 major groups consistent with type and pedigree information. The microsatellite profile database of maize varieties could be used to select comparative varieties through genetic relationship analysis between existing varieties and candidate varieties in distinctness tests.

Identification of New Microsatellite DNAs in the Chromosomal DNA of the Korean Cattle (Hanwoo)

  • Kim, J.W.;Hong, J.M.;Lee, Y.S.;Chae, S.H.;Choi, C.B.;Choi, I.H.;Yeo, J.S.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.17 no.10
    • /
    • pp.1329-1333
    • /
    • 2004
  • To isolate the microsatellites from the chromosomal DNA of the Korean cattle (Hanwoo) and to use those for the genetic selection, four bacteriophage genomic libraries containing the chromosomal DNA of six Hanwoo steers showing the differences in meat quality and quantity were used. Screening of the genomic libraries using $^{32}P-radiolabeled 5'-({CA})_{12}-3$nucleotide as a probe, resulted in isolation of about 3,000 positive candidate bacteriophage clones that contain $(CA)_n$-type dinucleotide microsatellites. After confirming the presence of microsatellite in each positive candidate clone by Southern blot analysis, the DNA fragments that include microsatellite and flanking sequences possessing less than 2 kb in size, were subcloned into plasmid vector. Results from the analysis of microsatellite length polymorphism, using twenty-two PCR primers designed from flanking region of each microsatellite DNA, demonstrated that 208 and 210 alleles of HW-YU-MS#3 were closely related to the economic traits such as marbling score, daily gain, backfat thickness and M. longissimus dorsi area in Hanwoo. Interestingly, HW-YU-MS#3 microsatellite was localized in bovine chromosome 17 on which QTLs related to regulation of the body fat content and muscle ypertrophy locus are previously known to exist. Taken together, the results from the present study suggest the possible use of the two alleles as a DNA marker related to economic trait to select the Hanwoo in the future.

Genetic structure of Larimichthys polyactis (Pisces: Sciaenidae) in the Yellow and East China Seas inferred from microsatellite and mitochondrial DNA analyses

  • Kim, Jin-Koo;Min, Gi-Sik;Yoon, Moon-Geun;Kim, Yeong-Hye;Choi, Jung-Hwa;Oh, Taeg-Yun;Ni, Yong
    • Animal cells and systems
    • /
    • v.16 no.4
    • /
    • pp.313-320
    • /
    • 2012
  • Genetic variation was surveyed at four microsatellite loci and 1416 base pairs (bp) of the mitochondrial DNA (mtDNA) cytochrome c oxidase I gene (COI) to clarify the genetic structure of the small yellow croaker, Larimichthys polyactis, in the Yellow and East China Seas, especially regarding four provisional populations, (one Korean and three Chinese populations). Based on microsatellite DNA variations, the estimated expected heterozygosity ($H_E$) in each population ranged from 0.776 to 0.947. The microsatellite pairwise $F_{ST}$ estimates showed no significant genetic differentiation between the populations. MtDNA variations also indicated no genetic structure in L. polyactis, but very high variability. The absence of genetic differentiation among and within populations of L. polyactis may either result from the random migration of the adult or the passive dispersal of the eggs and larvae.

Analysis of Microsatellite DNA Polymorphisms for Pedigree Verification in Kyungju Dog(Dongkyung-i). (경주개(동경이)의 혈통확인을 위한 microsatellite DNA 다형성 분석)

  • Lee, Eun-Woo;Choi, Seog-Gyu;Cho, Gil-Jae
    • Journal of Life Science
    • /
    • v.18 no.6
    • /
    • pp.902-906
    • /
    • 2008
  • In this study, we analyzed the microsatellite DNA poly-morphisms for pedigree verification in Kyungju dog (Dongkyung-i) which is one of the Korean breed dogs. A total of 51 Dongkyung-i samples were genotyped using 8 microsatellite markers. The number of alleles observed at single locus ranged from 4 to 12, with average number of alleles per locus of 8.5. The expected heterozygosity and polymorphic information contents (PIC) values of the 8 microsatellite loci were $0.6162{\sim}0.8746$ (mean 0.7587) and $0.5461{\sim}0.8512$ (mean 0.7167), respectively. Of the 8 markers, PEZ3, PEZ6, PEZ12 and FHC2054 loci had relatively high PIC values (>0.7) in Dongkyung-i. Pedigree verification of Dongkyung-i was analyzed based on alleles observed. The results of the parentage testing were noted significant differences compared with breeders. These results show basic information of conservation and research in Dongkyung-i, and further studies of genetic pedigree in Dongkyung-i will be needed.

Construction of a Microsatellite Marker Database of Commercial Pepper Cultivars (유통 중인 고추 품종에 대한 Microsatellite 마커 Data Base 구축)

  • Kwon, Yong-Sham;Hong, Jee-Hwa;Choi, Keun-Jin
    • Horticultural Science & Technology
    • /
    • v.31 no.5
    • /
    • pp.580-589
    • /
    • 2013
  • This study was carried out to evaluate the suitability of microsatellite markers for varietal identification and genetic relationship of 170 commercial pepper cultivars. The relationship between marker genotypes and 11 pepper cultivars with different morphological traits was also analyzed. Of the 302 pairs of microsatellite primers screened against 11 pepper cultivars, 24 pairs were highly polymorphic in terms of number of alleles. These markers were applied for the construction of DNA profile data base for 170 commercial pepper cultivars. A total of 164 polymorphic amplified fragments were obtained from 24 microsatellite primers. The average polymorphism information content was 0.673 ranging from 0.324 to 0.824. One hundred and sixty four microsatellite alleles were used to calculate Jaccard's distance coefficients using unweighted pair group method. A clustering group of varieties, based on the results of microsatellite analysis, were categorized into 3 major groups corresponding to morphological traits. The phenogram discriminated all varieties by markers genotypes. These microsatellite markers will be useful as a tool for protection of plant breeders' intellectual property rights through variety identification in distinctness, uniformity and stability test.

Genotyping of avian pathogenic Escherichia coli by DNA fragment analysis for the differences in simple sequence repeats

  • Han, Mi Na;Byeon, Hyeon Seop;Han, Seong Tae;Jang, Rae Hoon;Kim, Chang Seop;Choi, Seok Hwa
    • Korean Journal of Veterinary Service
    • /
    • v.41 no.4
    • /
    • pp.257-262
    • /
    • 2018
  • Avian pathogenic E. coli (APEC) causes severe economic losses in the poultry farms, due to systemic infections leading to lethal colisepticemia. It causes a variety of diseases from air sac infection to systemic spread leading to septicemia. Secondary infection contains opportunistic infections due to immunosuppression disease. Collibacillosis causes the great problems in the poultry industry in Korea. Thus, it is necessary to identify and classify the characteristics of E. coli isolate of chicken origin to confirm the diversity of symptoms and whether they are transmitted among the farms. Fragment analysis is identify the difference in the number of Variable-Number Tandem-Repeats (VNTRs) for genotyping. VNTRs have repeating structure (Microsatellite, Short tandem repeats; STR, Simple sequence repeats; SSR) in the chromosome. This region can be used as a genetic marker because of its high mutation rate. And various lengths of the amplified DNA fragment cause the difference in the number of repetition of the DNA specific site. The number of repetition sequences indicates the separated size of fragments, so the each fragments can be distinguished by specific samples. The results of the sample show that there is no difference in six microsatellite loci (yjiD, aidB, molR_1, ftsZ, b1668, yibA). There are differences among the farms in relation of the number of repetitions of other six microsatellite loci (ycgW, yaiN, yiaB, mhpR, b0829, caiF). Four (ycgW, yiaB, b0829, caiF) of these six microsatellite loci show statistically significant differences (P<0.05). It means that the analysis using four microsatellite loci including ycgW, yiaB, b0829, and caiF can confirm among the farms. Five E. coli samples in one farm have same SSR repetition at all markers. But, there are significant differences from other farms at Four (ycgW, yiaB, b0829, caiF) microsatellite loci. These results emphasize again that the four microsatellite loci makes a difference in the amplified DNA fragments, enabling it to be used for E. coli genotyping.

High-Throughput DNA Extraction Method for Marker Analysis in Rice Grain (대량의 쌀 시료 분석을 위한 DNA 추출법)

  • Choi, Young-Deok;Lee, Hae-Kwang;Lee, Yun-Suk;Yun, Jeong-Hee;Kim, Su-Jeong;Park, Seong-Whan
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.51 no.spc1
    • /
    • pp.269-273
    • /
    • 2006
  • The study of molecular markers to improve crops largely depends on the availability of rapid and of efficient DNA extraction methods. Here we developed a cheap and convenient method to isolate genomic DNA from rice grains suitable for large-scale microsatellite analysis. We confirmed that the isolated rice DNA is suitable for PCR analysis with STS marker and SNP marker, as well as microsatellite marker. Further, we established high-throughput DNA extraction system in a 96-well plate format which make it possible high-throughput analysis of microsatellite markers with rice grains. This implies that the new method could be a useful tool for other types of marker analysis in large scale.