• Title, Summary, Keyword: Matrix metalloproteinase

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Inhibitory Effect of Siderophore Purified from Burkholderia sp. CAS-5 on the Matrix Metalloproteinase-2 (Gelatinase A) (Burkholderia sp. CAS-5 균으로 부터 생산된 시드로포어의 Matrix metalloproteinase-2(Gelatinase A) 억제 활성)

  • Kim, Kyoung-Ja
    • YAKHAK HOEJI
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    • v.50 no.4
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    • pp.228-233
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    • 2006
  • Matrix metalloproteinase-2 is known to be involved in pathological processes such as tumor invasion or rheumatoid arthritis. A soil microorganism producing siderophore under low iron stress $(up\;to\;5\;{\mu}m\;of\;iron)$ was identified as Burkholderia sp. Hydroxamate type siderophore produced by Burkholderia sp. CAS-5 was partially purified. MMP inhibitory activity of siderophore was confirmed by gelatin zymography. The $Zn^{2+}-chelating$ activity of siderophore correlated with the inhibition of MMP-2 activity.

Matrix Metalloproteinase: Inhibitory Effect of Marine Substances on MMP-2 and MMP-9

  • Nguyen, Van-Tinh;Qian, Zhong-Ji;Jung, Won-Kyo
    • Journal of the Chosun Natural Science
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    • v.4 no.4
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    • pp.255-265
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    • 2011
  • Marine ecosystems are often characterized by a high biological diversity, and it corresponds to a high chemical diversity. Up to present, more than 20,000 new bioactive substances have been isolated from marine organisms, where considerable numbers of these naturally occurring derivatives are developed as potential candidates for pharmaceutical application. In this process, screening of natural products from marine organisms that could potentially inhibit the expression of metalloproteinases has gained a huge popularity. Cancer is considered as one of the deadliest diseases in the medical field. Matrix metalloproteinase (MMPs) can degrade extracellular matrix (ECM) components and play important roles in a variety of biological and pathological processes. Matrix metalloproteinase inhibitors (MMPIs) have been identified as potential therapeutic candidates for metastasis, arthritis, chronic inflammation and wrinkle formation.

Expression of Matrix Metalloproteinase-2 and -13 and Tissue Inhibitor of Metalloproteinase-4 in Varicose Veins

  • Chang, Jee-Won;Maeng, Young-Hee;Kim, Su-Wan
    • The Korean Journal of Thoracic and Cardiovascular Surgery
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    • v.44 no.6
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    • pp.387-391
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    • 2011
  • Background: The relationship between the degree of expression of matrix metalloproteinases or tissue inhibitor of metalloproteinases and venous reflux remains to be investigated. Materials and Methods: Primary varicose vein tissues were obtained from 23 patients, 18 females and 5 males, aged from 19 to 73. Cephalic or basilic veins were obtained for the control group from 10 patients who underwent vascular access for maintenance hemodialysis. Two operative techniques (high ligation with stripping or endovenous laser coagulation) were used. The expression of matrix metalloproteinase-2 and 13 and tissue inhibitor of metalloproteinase-4 in the varicose vein group and control group was assessed semi-quantitatively by immunohistochemical slides stained with primary antibodies. Results: Twenty (87%) of the varicose vein group patients had greater or lesser saphenous vein diseases with reflux. The focal weak (+) stain for matrix metalloproteinases-2, and 13, and tissue inhibitor of matrix metalloproteinase-4 was dominant in the varicose vein group; the focal or diffuse strong stain (++ or +++) was prevalent in the control group. The differences were statistically significant (p<0.01). The degree of reflux and the duration of symptoms were not significantly related to the expression of MMP-13 (p=0.317 and p=0.654, respectively). Conclusion: Further study should be performed to investigate the relationship between the clinical characteristics related to venous hypertension or reflux and expression of MMPs and TIMP in varicose veins.

Secretory Differentiation of Hamster Tracheal Epithelial Cells Increases Activation of Matrix Metalloproteinase-2

  • Shin, Chan-Young;Lee, Woo-Jong;Park, Kyu-Hwan;Ryu, Jae-Ryun;Ko, Kwang-Ho
    • Biomolecules & Therapeutics
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    • v.12 no.1
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    • pp.1-8
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    • 2004
  • In chronic airway inflammatory diseases such as asthma and chronic bronchitis, it has been suggested that matrix metalloproteinases secreted from infiltrating neutrophil contribute the pathogenesis of the disease and have been a focus of intense investigation. We report here that hamster tracheal surface epithelial goblet cells (HTSE cells) produce matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2). Matrix metalloproteinase activities were investigated using [$^3H$]collagen-digestion assay and gelatin zymography. The subtype of matrix metalloproteinases expressed from HTSE cells was MMP-2 (gelatinase A), which was determined by Western blot with various subtype selective anti-matrix metalloproteinase antibodies. The MMP-2 and TIMP-2 cDNAs from HTSE cells were partially cloned by RT-PCR and they reveal more than 90% of sequence homology with those from human, rat and mouse. The collagenolytic activity was increased with the secretory differentiation of the HTSE cell and it was found that zymogen activation was responsible for the increased MMP-2 activity in HTSE cells. The results from the present study suggest that the metaplastic secretory differentiation of airway goblet cells may affect chronic airway inflammatory process by augmenting the zymogen activation of MMP-2.

Effects of Bambusae Caulis in Taeniam Extract on the UVB-induced Cell Death, Oxidative Stress and Matrix Metalloproteinase 1 Expression in Keratinocytes (각질세포에서 자외선B가 유도한 세포 사멸, 산화적 스트레스 및 matrix metalloproteinase 1 발현에 대한 죽여추출물의 영향)

  • Seok, Jin Kyung;Kwak, Jun Yup;Seo, Hyeong Ho;Suh, Hwa Jin;Boo, Yong Chool
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.1
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    • pp.9-20
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    • 2015
  • Ultraviolet radiation (UV) is a major cause of skin photoaging, and effective UV protecting agents are needed for the skin health and beauty. This study was undertaken to examine the effects of Bambusae caulis in Taeniam extract (BCTE) on UVB-induced cell death, oxidative stress and matrix metalloproteinase 1 (MMP1) expression in cell-based assays. HaCaT human keratinocytes were exposed to UVB in the presence of BCTE at different concentrations and resulting changes in cell viability and biochemical events were determined. The results showed that BCTE enhanced the viabilities of UVB-exposed cells, and attenuated apoptotic events such as cleavage of procaspase 3 to its active form, and the increase of Bax to Bcl-2 ratios. BCTE also attenuated the reactive oxygen generation and lipid peroxidation in cells exposed to UVB. Additionally, it attenuated the expression of matrix metalloproteinase 1 and the phosphorylation of c-Jun N-terminal kinase stimulated by UVB. Conclusively, the present study demonstrated that BCTE pro tected skin cells from the UVB-induced cell death, oxidative stress and MMP1 expression, suggesting its potential use as a cosmetic ingredient mitigating some features of the skin photoaging.

Inhibitory Activity of Plant Extracts on Matrix Metalloproteinase-2 (식물추출물의 Matrix Metalloproteinase-2에 대한 저해활성)

  • Lee, Sang-Myung;Chun, Hyo-Kon;Lee, Ho-Jae;Lee, Choong-Hwan;Kim, Jin-Hee;Kho, Yung-Hee
    • Korean Journal of Pharmacognosy
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    • v.33 no.2
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    • pp.137-143
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    • 2002
  • The inhibitory effects of methanol extracts of 165 medicinal herbs on matrix metalloproteinase-2 (MMP-2) activity were investigated. Amongst the herbal extracts, Youngia denticulate, Platycarya strobilacea, Sedum kamtschaticum, Euscaphis japonica, and Comus controversa showed a significant inhibitory effect with above 80% at $1\;{\mu}g/ml$. Above mentioned five kind extracts, which exclude polyphenols and/or tannin by techniques for their removal, showed a mild inhibitory effect on MMP-2 activity except for Y. denticulate at the same concentration.

Adenovirus-Mediated Gene Delivery of Tissue Inhibitor of Metalloproteinase-1 Inhibits Migration of B16F10 Melanoma Cell in Wound Migration Assay

  • Seungwan Jee;Hoil Kang;Park, Sehgeun;Park, Misun;Miok Eom;Taikyung Ryeom;Kim, Okhee
    • Proceedings of the Korean Society of Toxicology Conference
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    • pp.177-177
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    • 2003
  • Tumor cell invasion and metastasis are a complex multistep process that involves the degradation of extracellular matrix proteins by matrix metalloproteinases (MMPs). Tissue inhibitor of metalloproteinase-1 (TIMP-1) acts as a negative regulator of matrix metalloproteinase and thus prevents tumor cell invasion and metastasis by preserving extracellular matrix integrity.(omitted)

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Effect of Curcumin on Cancer Invasion and Matrix Metalloproteinase-9 Activity in MDA-MB-231 Human Breast Cancer Cell (Curcumin이 인체 유방암세포 MDA-MB-231 Cell의 전이 과정과 Matrix Metalloproteinase-9 활성에 미치는 영향)

  • Bang, Myung-Hee;Kim, Woo-Kyoung
    • Journal of Nutrition and Health
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    • v.39 no.8
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    • pp.756-761
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    • 2006
  • Curcumin has been known for its anti-proliferative and apoptotic effects on several cancer cells. We examined the inhibitory effects of curcumin on cancer cell adhesion, motility, invasion and matrix metalloproteinase-9 (MMP-9) activity in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured with 0, 5, 10 or $20{\mu}M$ of curcumin. Curcumin significantly inhibited the adhesion of cancer cells to the fibronectin at $20{\mu}M$ and suppressed the motility and invasion of cancer cells at all concentrations. Also, the MMP-9 activity was inhibited by curcumin, but MMP-9 protein amounts were not affected. Our data indicate that curcumin inhibits motility, invasion and MMP-9 activity of MDA-MB-231 cells. Therefore, curcumin may contribute to the potential beneficial food component to prevent the cancer metastasis in human breast cancer.

A study on separation and characterization of matrix metalloproteinase-9 inhibitors from natural plants (천연 식물 추출물에서 Matrix Metalloproteinase-9 활성 억제제의 분리 및 특성화에 관한 연구)

  • Hur, Yong-Chul;Park, Sung-Woo;Kim, Tai-Jin
    • Analytical Science and Technology
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    • v.18 no.3
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    • pp.188-193
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    • 2005
  • Three different oriental natural plants (Angelicae Dahuricae Radix, Sanguisorba Officinalis L, Euonymus alatus) were extracted with 70% methanol under refluxing for 4 hr in order to investigate their inhibitory effect on Matrix Metalloproteinase-9 (MMP-9) by a modified gelatin zymography, where only euonymus alatus showed the inhibitory effect on the activity of Matrix MMP-9. The fraction was collected by using the mixture of ethyl acetate and hexane on silica gel column. Seven portions were obtained and three fractions of them (first, third and forth) showed inhibitory effect on the zymography. To verify the effect of these substances on cells, human hepatoma, Hep3B cells as a cancer model, and Chang liver cells as a normal model were selected. In order to examine the cell viability, $1{\mu}g/mL$ of each extract was treated on cells. Most of the methanol soluble fractions showed negligible toxicity on human liver cell line.

TIMP-2 Overexpression Suppresses Migraion, Invasiveness and Angiogenesis

  • Ahn, Seong-Min;Yeowon Sohn;Kim, Yun-Soo;Aree Moon
    • Proceedings of the Korean Society of Toxicology Conference
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    • pp.139-139
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    • 2002
  • An imbalance between matrix metalloproteinase (MMP)-2 and its endogenous inhibitor, tissue inhibitor of metalloproteinase (TIMP)-2 causes the degradation of the extracellular matrix associated with pathological events including invasion, metastasis and angiogenesis.(omitted)

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