• Title/Summary/Keyword: MLS resistance

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Characterization of S. aureus Showing MLS Resistance with Foggy D Shape (fMLS) (안개 D형 저지원을 갖는 MLS 내성 황색 포도상 구균의 분포와 내성 기전)

  • Yoon, Eun-Jeong;Kim, Hyun-Jee;Kwon, Ae-Ran;Choi, Sung-Sook;Shim, Mi-Ja;Choi, Eung-Chil
    • YAKHAK HOEJI
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    • v.50 no.3
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    • pp.199-203
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    • 2006
  • A new type of macrolides, lincosamides and streptogramin B antibiotics (MLS) resistance, showing the characteristic phenotype growing within the inhibition zone area around the clindamycin disk, was identified among clinically isolated Staphylococcus aureus. We named the phenotype as MLS resistance with foggy-D shape (fMLS). The average frequency of MLS isolates was 9%. All of fMLS isolates have only erm(A) for the resistance determinant. The growth pattern of the challenged MLS isolate looks intermediate phase between the patterns of inducible MLS resistance and constitutive MLS resistance.

Nucleotide Sequence and Properties of Macrolide-Lincosamide-Streptogramin B Resistance Gene from Staphylococcus aureus DH1 (Staphylococcus aureus DH1에서 분리된 Macrolide-Lincosamide-Streptogramin B 계열 항생물질에 대한 저항성 인자의 특성과 염기서열)

  • 권동현;박승문;윤권상;변우현
    • Korean Journal of Microbiology
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    • v.28 no.1
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    • pp.27-34
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    • 1990
  • Two macrolide-lincosamide-streptogramin B (MLS) antibiotic resistance genes, one expressed inducibly and the other expressed constitutively were recognized from a single Staphylococcus aureus DH1 strain. The inducible MLS resistance gene was isolated and cloned from the R-plasmid pDE1(7.4kb) and the constitutive gene was from chromosomal DNA. Base sequence of the inducible MLS resistance gene (1.2kb) was determined and found as same that of pE194. The restriction map of the cloned constitutive MLS resistance gene was compared with that of the inducible gene. Two genes have same restriction map except leader region. In the constitutive gene there is no leader region which is doing major role in inducible expression.

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R-plasmids in staphylococcus aureus (Staphylococcus aureus의 항생제 내성 plasmid에 관한 연구)

  • 변우현;김영선;조은희;권동현;이호주;홍순주
    • Korean Journal of Microbiology
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    • v.23 no.4
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    • pp.282-290
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    • 1985
  • Small size antibiotic resistance plasmids having molecular weights less than 10 Mdal were isolated and characterized from ten clinically isolated multiple resistant Staphylococcus aureus. Agarose gel electrophoresis profiles and antibiotic resistance patterns divided these strains into four groups. Strain 2-23-6, the representative strain of a group of five strains conferred two plasmids of molecular weights $1.6{\times}10^6\;dal\;and\;2.0{\times}10^6$ dal. The small plasmid (pSBK 112) specified macrolides, lincosamides and streptogramin type B (MLS) resistance gene which are expressed constitutively. Lage plasmid (pSBK 125) specified chloramphenicol resistance gene which is inducible. Strain 10-5 conferred a $3.0{\times}10^6$ dal plasmid (pSBK 141) which carry an inducible ampicillin resistance gene and strain P-H-2 conferred and $1.6{\times}10^6$ dal plasmid (pSBK 190) which carry a constitutive MLS resistance gene. Strain D-H-1 conferred four plasmids of molecular weights $0.8{\times}10^6$ dal (pSBK 201), $1.6{\times}10^6$ dal (pSBK 202), $2.5{\times}10^6$ dal (pSBK 203), and $1.2{\times}10^7$ dal (pDBK 204), respectively. Among those four plasmids, only pSBK 203 specified chloramphenicol resistance gene. Curing of constitutive MLS resistance using acriding orange or ethidium bromide in 2-23-6 and P-H-2 strains produced 'inducible' MLS resistance strains which are less resistant to MLS than the wild type strains, suggesting that there are two resistance genes in both strains; one is constitutive and the other is inducible.

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MLS Inducible Resistance Mechanism in Bacillus licheniformis EMR-1 -Cloning of erm K, a MLS Resistance Determinant- (Bacillus licheniformis EMR-1에서의 MLS 유도내성 기전 -erm K의 크로닝-)

  • Choi, Eung-Chil;Kwak, Jin-Hwan;Weisblum, Bernard
    • YAKHAK HOEJI
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    • v.32 no.4
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    • pp.213-221
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    • 1988
  • Inducible MLS resistance gene of Bacillus licheniformis specified by erm K was subcloned in Bacillus subtilis and the DNA sequence corresponding to its control region was determined. The determinant erm K was in Pvu II=Hind III fragment, which was 1.3 kb. The leader region is capable of forming a complex series of inverted complementary repeat sequences (ICRS) centering on at least six axes of symmetry, some of them mutually exclusive, in a way that resulted ultimately in post-transcriptional unmasking of the ribosome loading site for methylase synthesis.

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a possible combined attenuation control of the inducible MLS resistance

  • ;Weisblum, B.
    • The Microorganisms and Industry
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    • v.13 no.2
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    • pp.8-13
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    • 1987
  • 이글에서는 항생물질 내성의 생화학적 기전, MLS계 항생물질 내성기전, Post-transcriptional attenuation control, MLS계 항생물질 내성기전 연구방향, erm K의 cloning: transcriptional attenuation control의 가능성에 대해 논하였다.

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Cloning of Inducible MLS Antibiotics Resistance Genes and their Expression Control Mechanism - ermC-4, a macrolide-lincosamide-streptogramin B resistance determinant on pMB4 from Staphylococcus aureus TR-1 (MLS계 항생물질 유도내성 유전자의 크로닝과 유전자의 발현조절 기전 - Staphylococus aureus TR-1균주의 프라스미드 pMB4에 존재하는 MLS 내성 유전자 ermC-4)

  • 김수환;최응칠;김병각;심미자
    • YAKHAK HOEJI
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    • v.35 no.1
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    • pp.22-29
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    • 1991
  • pMB4 is a 2.4-kilobase plasmid of Staphylococcus aureus TR-1 that confers inducible resistance to the macrolide-lincosamide-streptogramin B(MLS) antibiotics. By subcloning studies, it was found that the MLS resistance determinant was located at 1.0Kb fragment between Sau3AI and TaqI sites. DNA sequence of the MLS resistant determinant, named ermC-4 was determined, and found to be highly homologous with that of ermC. Because the leader peptide sequence of ermC-4 was identical with that of ermC, the expression of the resistance gene is thought to be controlled by posttranscriptional attenuation in S. aureus TR-1.

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Screening of Novel Inducible Resistance Gene to Macrolide-Lincosamide-Streptogramin B (MLS) Antibiotics from Clinical Isolates of Staphylococcus spp (임상분리 Staphylococcus속 균주로부터 마크로라이드-린코사마이드-스트렙토그라민 B(MLS)계 항생물질에 대한 새로운 유도내성 유전자의 검색)

  • 오정자;권애란;이미정;김숙경;최성숙;최응칠;김병각
    • Biomolecules & Therapeutics
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    • v.1 no.2
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    • pp.177-182
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    • 1993
  • From 84 clinical isolates of Staphylococcus species, ten strains showing inducible resistance to MLS antibiotics were selected by disk agar diffusion method. Colony hybridization was executed using two MLS inducible resistance genes, ermA and ermC, previously identified from S. aureus as probes. S. hemolyticus 401 and S. epidermidis 542 whose genes were not homologous to those probes were finally selected. It was determined that the resistance genes of S. hemolyticus 401 and S. epidermidis 542 were not homologous to ermA, ermC and ermAM by Southern hybridization. S. epidermidis 542 had a plasmid DNA. To know if the plasmid may have genes related to inducible resistance, it was attempted to transform B. subtilis BR151 and S. aureus RN4220 with the plasmid prepared from S. epidermidis 542. It was shown that the gene related to inducible resistance to MLS antibiotics did not exist in this plasmid. These results indicate that two clinical isolates of S. hemolyticus 401 and S. epidermidis 542 had novel genes which were not homologous to MLS resistance genes identified previously. It was assumed that these genes may exist in chromosomal DNA.

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Screening of Inducible Resistance Genes to Macrolide-Lincosamide-Streptogramin B(MLS) Antibiotics (마크로라이드-린코사마이드-스트렙토그라민 B(MLS)계 항생물질에 대한 유도 내성)

  • Kwon, Ae-Ran;Choi, Sung-Sook;Kim, Sook-Kyung;Chung, Young-Ja;Choi, Eung-Chil;Kim, Byoung-Kak
    • YAKHAK HOEJI
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    • v.38 no.3
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    • pp.293-299
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    • 1994
  • Forty nine clinical isolates of S. aureus showing resistance to erythromycin(EM) were selected from 83 strains isolated recently in Korea. Fourteen strains of S. aureus showing inducible resistance to MLS antibiotics were selected by disc agar diffusion method. Colony hydridization was executed using two MLS inducible resistance genes, ermA and ermC, identified previously from S. aureus as probes. S. aureus 375 and S. aureus 507 whose genes were not homologous to those probes were finally selected. It was confirmed that the resistance genes of S. aureus 375 and S. aureus 507 had no homology with those probes in southern hybridization test using ermA, ermC and ermAM as probes. It was determined that S. aureus 375 had a plasmid whose size was about 35 kb. To know if the plasmid may have the genes related to inducible resistance to MLS antibiotics, it was attempted to transform Bacillus subtillis BR151 and S. aureus RN4220 with the plasmid isolated from S. aureus 375. It was shown that the gene related to inducible resistance to MLS antibiotics did not exist in this plasmid. These results indicate that two clinical isolates of S. aureus showing inducible resistance to MLS antibiotics have novel genes that have no homology with MLS resistance genes identified so far. It is assumed that these genes may exist in chromosomal DNA.

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