• Title, Summary, Keyword: M88

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mRNA Expression of Bax, Bcl-2, p53, Cathepsin B, Caspase-3 and Caspase-9 in the HepG2 Cell Line Following Induction by a Novel Monoclonal Ab Hep88 mAb: Cross-Talk for Paraptosis and Apoptosis

  • Mitupatum, Thantip;Aree, Kalaya;Kittisenachai, Suthathip;Roytrakul, Sittiruk;Puthong, Songchan;Kangsadalampai, Sasichai;Rojpibulstit, Panadda
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.2
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    • pp.703-712
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    • 2016
  • Monoclonal antibodies with specific antigens have been widely used as targeted therapy for cancer. Hep88 mAb is a monoclonal antibody which shows specific binding with anti-cancer effects against the HepG2 cell line. However, its mechanisms of action are still not completely understood. We examined cell cycling and apoptosis by flow cytometry and mRNA expression of factors involved in apoptosis and paraptosis in Hep88 mAb-treated HepG2 cells by real-time PCR. The cell-cycle analysis demonstrated that growth-inhibitory activity was associated with G2/M cell cycle arrest. Hep88 mAb induced a significant increase in apoptotic cell populations in a dose- and time-dependent manner. The mRNA expression results also suggested that the process triggered by Hep88 mAb involved up-regulation of tumor suppressor p53, pro-apoptotic Bax, Cathepsin B, Caspase-3 and Caspase-9, with a decrease of anti-apoptotic Bcl-2 - thus confirming paraptosis and apoptosis programmed cell death. These findings represent new insights into the molecular mechanisms underlying the anti-cancer properties of Hep88 mAb in liver cancer cells.

Hep88 mAb-Mediated Paraptosis-Like Apoptosis in HepG2 Cells via Downstream Upregulation and Activation of Caspase-3, Caspase-8 and Caspase-9

  • Mitupatum, Thantip;Aree, Kalaya;Kittisenachai, Suthathip;Roytrakul, Sittiruk;Puthong, Songchan;Kangsadalampai, Sasichai;Rojpibulstit, Panadda
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.5
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    • pp.1771-1779
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    • 2015
  • Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide. Presently, targeted therapy via monoclonal antibodies to specific tumor-associated antigens is being continuously developed. Hep88 mAb has proven to exert tumoricidal effects on the HepG2 cell via a paraptosis-like morphology. To verify the pathway, we then demonstrated downstream up-regulation of caspase-3, caspase-8 and caspase-9, assessingmRNA expression by real-time PCR and associated enzyme activity by colorimetric assay. Active caspase-3 determination was also accomplished by flow cytometry. Active caspase-3 expression was increased by Hep88 mAb treatment in a dose-and time-dependent manner. All of the results indicated that Hep88 mAb induced programmed cell death in the HepG2 cell line from paraptosis-like to apoptosis by downstream induction of caspases. These conclusions imply that Hep88mAb might be a promising tool for the effective treatment of HCC in the future.

Biodegradation of Naphthalene by Acinetobacter calcoaceticus R-88 (나프탈렌 분해균주의 분리 및 특성)

  • Ryu, Beung-Ho;Oh, Yun-Kun;Bae, Ki-Chul;Bin, Jae-Hoon
    • Applied Biological Chemistry
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    • v.32 no.3
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    • pp.315-320
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    • 1989
  • Bacteria utilizing naphthalene as a sole carbon source for growth were isolated and identified and code named as Acinetobacter calcoaceticus R-88, Pseudomonas testosteroni R-87 and Pseudomonas putida R-89. Among these isolates, A. calcoaceticus R-88 found most effective in utilizing naphthalene. The optimal pH, temperature and concentration of naphthalene was 7.0, $30^{\circ}C$ and 10mM, respectively. The strain degraded naphthalene to salicylic acid as an intermediate. And the strain showed to be resistant to ampicillin, tetracyclin, chloramphenicol and kanamycin. A. calcoaceticus R-88 harbored plasmid DNA which was believed to be involved in naphthalene degradation.

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Expression of Matrix Metalloproteinases (MMPs) and Their Tissue Inhibitors (TIMPs) in Frozen Sperm of Rabbit (동결융해 후 토끼 정자의 Matrix Metalloproteinases (MMPs)와 Their Tissue Inhibitors (TIMPs) 발현 양상)

  • Kim, Sang Hwan;Choi, Hwa Sik;Yoon, Jong Taek
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.3
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    • pp.247-252
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    • 2019
  • We observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. According to the results from each freezing extender, the sperm membrane integrity (HOST: Hypoosmotic Swelling Test) analysis in TCGGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Glycerol 3%, Dimethylsulpoxide 3.5 M) is 59.8 ± 0.7, TCGSD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Sucrose 0.1 M, Dimethylsulpoxide 3.5 M) is 59.3 ± 0.5 were significantly higher (p < 0.05) among the experimental groups. And MMPs analysis result, we observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. The expression of active MMP-2 was the highest in sperms frozen in TCGSD and TCGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Dimethylsulpoxide 3.5 M), Meanwhile, sperms from the TCGGD and TCGED (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Ethylene glycol 3%, Dimethylsulpoxide 3.5 M) group showed lower level of active MMP-2 expression. Together, these results indicate that adding glycerol or sucrose to the sperm freezing buffer would not only suppress MMPs expression but also minimize DNA fragmentation, providing a mean to improve the success rate in the in vitro manipulation of rabbit sperms. Therefore, these results suggest that TCGGD or TCGSD extender method for freezing-thawing of rabbit sperm increased the viability after thawing.

Studies on the Protoplast Formation and Regeneration of Lactobacillus acidophilus 88 (Lactobacillus acidophilus 88의 Protoplast 형성 및 재생에 관한 연구)

  • Jun, Hong-Ki;Heo, Kyeong;Jo, Young-Bae;Baik, Hyung-Suk
    • Microbiology and Biotechnology Letters
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    • v.22 no.2
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    • pp.143-151
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    • 1994
  • In the course of the study on strain inprovement by protoplast fusion, Lactobacillus acidophilus 88 protoplasts production and regeneration conditions were investigated. This strain produced a bacteriocin that revealed strong inhibitory activity against various indicator strains, especially L. helveticus CNRZ 1096. Protoplasts of L. acidophilus 88 strains were very efficiently obtained by treatment with 125 $\mu $g/ml lysozyme in a protoplast forming buffer containing 20 mM N-2 hydroxy-ethtl-piperazine-N'-2-ethane-sulfonic acid(HEPES, pH 7.0) and 1M sucrose at 37$\circ $C for 30 min. Hovever, treatment with mutanolysin was not effective for the production of L. acidophilus 88 protoplasts under the same conditions. High protoplast yield was obtained form the cells at the middle to late logarithmic growth phase in the de Man, Rogosa and Sharpe(MRS) medium. Regeneration was efficiently accomplished with the MRS medium containing 10% sucrose.

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Molecular Cloning of the cDNA of Heat Shock Protein 88 Gene from the Entomopathogenic Fungus, Paecilomyces tenuipes Jocheon-1

  • Liu, Ya-Qi;Park, Nam Sook;Kim, Yong Gyun;Kim, Keun Ki;Park, Hyun Chul;Son, Hong Joo;Hong, Chang Ho;Lee, Sang Mong
    • International Journal of Industrial Entomology
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    • v.28 no.2
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    • pp.71-84
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    • 2014
  • The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes) Jocheon-1 Uni-Zap cDNA library and performing 5' RACE polymerase chain reaction (PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of 2,139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88 and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminal region. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton (kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditions for the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1 HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stress induced a higher level of mRNA expression compared to normal growth conditions.

전차의 전투가치 향상-M1, Leopard2 및 M48을 중심으로

  • Min, Ga-Jin
    • Defense and Technology
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    • no.4
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    • pp.10-18
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    • 1991
  • 현재 우리나라가 보유하고 있는 전차는 주로 한국이 독자개발한 최신예 88전차와 M48전차로 구성되어 있다. 북괴에 대한 기갑전력의 열세를 만회하기 위해서는 북괴 대전차 무기체계를 분석하여 이에 효과적으로 대응할수 있는 방안을 찾아야 할 것이며, 이는 88전차와 M48계열 전차의 적절한 전투가치 향상사업으로 집약될 것이다. 특히 M48 계열 전차의 장갑 보강을 위해 덧붙임장갑(Add-on Armour)의 장착이 필수적이며, 화학에너지탄뿐 아니라 운동에너지탄으로부터의 방호를 위해 Hybrid 덧붙임장갑을 장착해야 될 것이다

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