• Title, Summary, Keyword: Liquid Semen

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Current Status of Swine Artificial Insemination(AI) in Korea (한국의 돼지 인공수정 현황)

  • Kim, In-Cheul;Sa, Soo-Jin;Kang, Kwon;Kim, Sang-Hyun;Bae, Sang-Jong;Kim, Dae-Sil;Kim, Si-Joo;Min, Chen-Sik;Son, Jung-Ho;Chung, Ki-Hwa
    • Reproductive and Developmental Biology
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    • v.35 no.3
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    • pp.227-232
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    • 2011
  • This survey was conducted to investigate the current status of swine artificial insemination(AI) centers registered as 'semen processing business' in Korea. The survey responses were collected by direct visitation or telephone conversation for 5 months from May through September in 2008. The survey showed that sixty-four AI centers were enrolled in local government and those of fifty-two AI centers were under operation. Forty-nine AI centers surveyed owned a total of 3,334 boars and the Duroc breed accounted for the highest rate(73.1%) of all boar breeds. In type of ownership, agricultural management corporations was the highest(42.3%) and followed by private ownership(34.6%). Large-scale AI centers in terms of own over 151 boar were surveyed as 5.9% and most AI centers own less than 100 boars(86.5%). The average number of boars per AI center was 68. The amount of liquid semen provided by 52 AI centers were 1,791,000 doses and each AI center provides average of 39,000 does, which is represented for 90% consumption by sows in Korea.

Effect of Cholesterol and Serum Albumin on Sperm Ability and Lipid Peroxidation during the Storage of Miniature Pig Sperm (미니돼지정액의 보존 시 콜레스테롤과 혈청 알부민이 정자 성상과 지질 과산화에 미치는 영향)

  • Kim, Dong-Woo;Lee, Yong-Seung;Yoo, Han-Jun;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Journal of Embryo Transfer
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    • v.26 no.1
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    • pp.71-78
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    • 2011
  • This study was undertaken to find out the effect of cholesterol and serum albumin on sperm ability and lipid peroxidation levels period to the liquid storage of miniature pig sperm. Ejaculated semen from miniature pigs was collected by gloved-hand method into a pre-warmed ($37^{\circ}C$) thermos bottle, and extended with Modena solution {with and without BSA, methyl-beta-cyclodextrin (-cholesterol) and cholesterol loaded cyclodextrin (+cholesterol)}. Each semen was assessed for viability (SYBR-14/PI staining) and acrosome intactness, intensity and capacitation status by chlorotetracycline (CTC) staining at 1, 3, 5, 7 and 10 days of storage. At for the effects of cholesterol and serum albumin on lipid peroxidation, semen were incubated with $H_2O_2$ ($10\;{\mu}M$), and lipid peroxidation level were measured by flow cytometry using the lipid peroxidation reporter probe $C_{11}-BODIPY^{581/591}$. The result, lipid peroxidation level in sperm added with cholesterol were lower in $10\;{\mu}M$ $H_2O_2$ compared to the added sperm with serum albumin. Also, added cholesterol to sperm had significant (p<0.05) higher viability when storage for 7 and 10 days and lower when 10 days of storage percentage of acrosome-reacted sperm (AR pattern) in acrosome state as say result compared to other treated groups. In conclusion, role of cholesterol during lipid storage in miniature pig spermatozoa was protected boar spermatozoa from lipid peroxidation prior to lipid storage. Addition serum albumin during lipid storage in sperm may be induce sperm membrane damage by lipid peroxidation. Therefore, addition of cholesterol to miniature pig sperm will be lead to extension of liquid storage periods.

An Analysis of Survey Data on South Korea Boar Stud Practices (한국의 돼지 인공수정센터 현황 분석)

  • Sa, Soo-Jin;Woo, Jae-Seok;Hong, Joon-Ki;Kim, Ki-Hyun;Kim, Du-Wan;Kim, Yong-Min;Park, Hyun-Sik;Kim, Si-Joo;Chung, Ki-Hwa;Cho, Eun-Seok
    • Journal of Embryo Transfer
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    • v.30 no.3
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    • pp.129-135
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    • 2015
  • The aim of this study is to examine current status of swine AI and boar stud in South Korea using survey and data analysis. This survey included 48 boar studs registered as 'semen processing business'. The survey data were collected by direct visitation, FAX and/or telephone conversation for 7 months from June through December in 2013. 48 boar studs owned a total of 3,537 boars and the Duroc breed accounted for the highest rate (75.3%) of all boar breeds. In case of ownership, agricultural management corporations was the highest (50.0%) and followed by individual ownership (33.3%). Large-scale boar studs in terms of own over 151 boar were surveyed as 4.2% and most boar studs owned less than 100 boars (77.1%). The amount of liquid semen provided by 48 boar studs were 1,889,000 doses and each boar stud provided average of 39,000 does, which is represented for 90% consumption by sows in South Korea.

Study on the Preservation of Liquid Boar Semen with $\textrm{BF}_5$ and Butschwiler Diluents (희석액 $\textrm{BF}_5$ 엔오투와 Butschwiler를 이용한 돼지 액상정액 보존에 관한 연구)

  • 천용민;박창식;서길웅;이규승
    • Journal of Embryo Transfer
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    • v.11 no.2
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    • pp.159-166
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    • 1996
  • 본 연구는 돼지 액상정액을 인공수정용 100ml 플라스틱 병에 보존하면서 BF5희석액과 Butschwiler 희석액 간에 보존 온도별 차이를 조사하고, BF5 희석액에서의 글리세롤 농도의 효과를 조사하여 돼지 액상정액을 좀더 장기간 사용할 수 있는 방법을 찾고자 실시하였다. 돼지 액상정액을 5$^{\circ}C$ 냉장고에 보존하면서 조사한 바에 의하면, 37$^{\circ}C$에서 0.5 및 2시간 배양후의 정자운동성은 전체 보존기간동안 BF5 희석액이 Butschwiler 희석액보다 유의하게 (P<0.05) 높게 나타났고, 정상첨체비율은 두 희석액간에 차이가 없었다. 돼지 액상정액을 15$^{\circ}C$에 보존하면서 조사한 바에 의하면, 3일부터 7일 보존시 까지 정자운동성과 정상첨체비율에 있어서 Butschwiler 희석액이 BF5 희석액보다 유의하데 높게 나타났다. BF5 희석액을 이용한 돼지 액상정액의 글리세롤 농도의 효과에 있어서는 최종 글리세롤 농도가 0, 2, 3, 및 5% 보다 1%일 때 가장 높은 정자운동성과 정상첨체비율을 나타내었다. 분만율, 복당 생존자돈수 그리고 출생시 평균 생시체중은 BF5 희석애과 Butschwiler 희석액간에 차이가 없었다. 이상의 연구 결과를 종합해 볼 때 BF5 희석액을 5$^{\circ}C$에서 Butschwiler 희석액은 15$^{\circ}C$에서 6-7일 동안 돼지 액상정액을 보존할 수 있었다.

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Effect of Packing Materials of Frozen Boar Semen on Sperm Characteristics and Reproductive Performance (동결정액 포장방법이 돼지정액의 성상 및 번식성적에 미치는 영향)

  • 김인철;이장희;김현종;이성호;박창식
    • Korean Journal of Animal Reproduction
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    • v.26 no.2
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    • pp.119-124
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    • 2002
  • This study was carried out to investigate the effects of packing materials of frozen boar semen to improve reproductive performance efficiency in pig. Boars were raised at Swine Artificial Insemination Center in National Livestock Research Institute, Sunghwan, Chungnam, Korea. We compared packing protocols for frozen boar semen among 5$m\ell$ maxi-straw, 5$m\ell$ cryogenic-vial, and aluminum-pack. Cryogenic-vial packing material showed similar sperm characteristics compared with maxi-straw packing material when the sperm was frozen above 15cm from liquid nitrogen and thawed at 52$^{\circ}C$ for 190 seconds. We investigated different thawing times to find out the optimal condition of freezing and thawing protocol with cryogenic-vial. Freezing above 15cm from liquid nitrogen and thawing at 52$^{\circ}C$ for 190 seconds were the optimal protocol compared with 120 and 150 seconds. However, normal acrosome rates did not show any differences among thawing times. Post-thawing results of maxi-straw in water at 52$^{\circ}C$ for 45 seconds had better total motility and curve linear velocity than those of cryogenic-vial in water 52$^{\circ}C$ for 190 seconds. However, there were no differences on straightness and normal apical ridge of sperm between maxi-straw and cryogenic vial. Non-return rate, farrowing rate and litter size of sows inseminated with frozen boar semen of commercial farms were higher in the maxi-straw than cryogenic-vial, but there were no significant differences between maxi-straw and cryogenic-vial. In conclusion, there were no significant differences between maxi-straw and cryogenic-vial and so, we may replace cryogenic-vial packing method instead of maxi-straw packing method by improvement of freezing and thawing rate.

Change of Sperm Viability and Acrosome Integrity of Post-thawed Korean Jeju Black Bull Spermatozoa according to Glycerol Concentration (제주 흑우 동결 정액 제조에 있어 Glycerol의 농도에 따른 생존율 및 정자 첨체 양상의 변화)

  • Choi, Sun-Ho;Ko, Min-Hee;Kang, Tae-Young;Cho, Sang-Rae;Park, Yong-Sang;Oh, Shin-Ae
    • Journal of Embryo Transfer
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    • v.26 no.3
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    • pp.187-193
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    • 2011
  • This study was carried out to establish most suitable freezing condition, to evaluate the different glycerol concentration of freezing and thawing rates on motility, viability, membrane integrity and acrosome intecrity of frozen Korean Jeju Black Bull spermatozoa, Semen was collected from a Korean Jeju Black Bull using an artificial vagina and transported to the laboratory. The semen was extended gradually 1:5 then cooled slowly for 2 hrs to 4$^{\circ}C$. The semen was diluted 1:1 with cryoprotectant extenders (3%, 5% and 7% glycerol) and equilibrated for 2 hrs at cold chamber and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 min and above 8 cm for 10 min. And then the frozen straw was plunged into LN$_2$. The presented straws were examined the viability and motility after thawed at 37$^{\circ}C$ water bath. The viability and membrane integrity immediately post-thawing were significantly higher in samples frozen in 7% glycerol than 3% and 5% glycerol (p<0.05). After CTC staining to assess acrosome integrity, F pattern was significantly increased, but B pattern was significantly decreased in 7% glycerol (p<0.05). Freezing distance of 5 cm from liquid nitrogen and pre-cooling for 10 min yield better survival and membrane integrity, but not significant difference. However, AR pattern according to CTC staining was significantly decreased in 3 cm for 5 min.

Physicochemical properties of Dongchimi added with . Jasoja(Perillae semen) (자소자첨가 동치미의 이화학적 특성)

  • 황재희;장명숙
    • Korean journal of food and cookery science
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    • v.17 no.6
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    • pp.555-564
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    • 2001
  • The optional ingredient jasoja(Perillae semen) was adopted to improve Dongchimi in qualify during fermentation The final weight percentage of jasoja in Dongchimi was adjusted to 0, 0.25, 0.5, 0.75, or 1.0% Per radish. Physicochemical characteristics were determined during fermentation for 45 days at 10$^{\circ}C$. As the fermentation of Dongchimi proceeded, the pH decreased with the increase of total acidity; however, the decreasing rate was slowed down by increasing the level of jasoja. The delayed pH drop of Dongchimi was limited only for the initial period of fermentation and the ultimate pH became almost comparable even for the 1% treatment. The total vitamin C content increased initially to a certain level depending on the level of jasoja, and then decreased later. Dongchimi with 0.5% jasoja contained the highest level of total vitamin C and reducing sugars. In case of 1% treatment, the typical pattern of an initial high content followed by a gradual decrease in reducing sugar was destroyed by a rapid fermentation at the later stage. Turbidity level, along with total solid contents of the liquid part of Dongchimi increased in all treatments as the fermentation proceeded although the extent was rather suppressed by jasoja. As a result of fermentation, the colorimetric lightness values decreased, with the intial increase followed by the decrease at a certain point in redness and yellowness and the increase in color difference values (ΔE). Overall, fermentation with 0.5% jasoja for 11 to 30 days appeared to improve the quality of Dongchimi.

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Development of Quantitative Extraction Method of Amygdalin without Enzymatic Hydrolysis from Tonin(Persicae Semen) by High Performance Liquid Chromatography

  • Lee, Sang-Soo;Hwang, Eun-Young;Koh, Jung-A;Kim, Dong-Min;Lee, Je-Hyun;Lee, Yong-Moon;Hong, Seon-Pyo
    • Proceedings of the PSK Conference
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    • pp.389.2-389
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    • 2002
  • Tonin(Persicae Semen)is the herb medicine that cntains amygdalin as a major ingredient. Amygdalin in water is decomposed into benzaldehyde. HCN. and glucoseby emulsin. a hydrolysis enzyme intonin. A useful and practical method for the optimum extraction condition of amygdalin without enzymatic hydrolysis is required. The extraction yield of amygdalin of natural formula tonin was 0.1 % from crude powers. 1.4% from small pieces. 3.5% from half pieces and 2.4% from whole pieces. (omitted)

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Quantitative Determination of Amygdalin Epimers from Armeniacae Semen by High Performance Liquid Chromatography.

  • Koo, Ja-Yong;Hwang, Eun-Young;Lee, Je-Hyun;Hong, Seon-Pyo
    • Proceedings of the PSK Conference
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    • pp.223.1-223
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    • 2003
  • D-Amygdalin and its conversion product, neoamygdalin, were clearly separated on reverse-phase column chromatography by an optimized eluent of 10 mM sodium phosphate buffer (pH 3.5) containing 8.5% acetonitrile. Linearity for analyzing D-amygdalin and neoamygdalin was observed in the range from 0.05 to 0.5 mM. The detection limits for D-amygdalin and neoamygdalin were ca. 5 uM per injected amount. When extracting amygdalin from a whole piece of Armeniacae Semen in the boiling aqueous solution, there was almost no influence of emulsin; it resulted in higher extraction yield. (omitted)

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Effect of Storage Times on Sperm Function, Sperm Chromatin Structure Assay (SCSA) and Correlations Between Fertility and SCSA in Boars (액상 정액의 보존 기간이 정자 기능 및 정자염색질 구조 분석에 미치는 영향과 인공수정 분만율과의 상관관계)

  • 유재원;이주형;김인철;이일주;강 권;민동수;윤희진;윤종택;방명걸;류범용;정영채;김창근
    • Journal of Animal Science and Technology
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    • v.48 no.6
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    • pp.785-796
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    • 2006
  • This study was designed to evaluate the changes in sperm motility, viability, HOST(hypo-osmotic swelling test), IVP(in vitro penetration), SCSA(sperm chromatin structure assay) during storage of liquid semen collected from boars with different farrowing rates using AI, and to find the relationship between boar fertility through AI and sperm diagnostic parameters during semen storage. The results of HOST were significantly decreased according to the increasing of in semen storage days and the results of IVP were significantly decreased at 3 days of semen storage (P<0.05). The %Red was significantly different among the >80%, 70󰠏80% and <70% farrowing rate group at semen storage day 6(P<0.05). The correlation coefficients between the %Red and farrowing rate were increased according to the semen storage. In conclusion, these results suggest that the sperm parameters evaluated in these studies may be useful indicators to predict the fertility of AI and evaluate the semen quality in boars.