• Title, Summary, Keyword: Larva

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Water Temperature, Rearing Density and Feeding Rate on Growth and Survival Rate of Red Marbled Rockfish, Sebastiscus tertius Larvae (수온, 자어밀도 및 먹이공급량에 따른 붉은쏨뱅이, Sebastiscus tertius 자어의 성장과 생존율)

  • 허성범;임상구;김철원;김광수
    • Journal of Aquaculture
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    • v.12 no.3
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    • pp.213-220
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    • 1999
  • This study was performed to investigate the effects of water temperature and stocking density and rotifer density on the mass production of larval red marbled rockfish, Sebastiscus tertius. Total length of the marbled rockfish larva grown in $23^{\circ}C$ of water temperature were 10.80mm, which was the best, while those grown in $29^{\circ}C$ of water temperature were 6.28mm, which was the lowest. The survival rates of red marbled rockfish larvae grown in $20^{\circ}C$ and $23^{\circ}C$ were 24.0% and 18.5%, respectively. However, the survival rate of red marbled rockfish larvae grown in $26^{\circ}C$ was $7^{\circ}C$ and no larvae survived in $29^{\circ}C$. The total length of red marvled rockfish larva stocked at the density of 5 and 10 larvaper liter of water were high, which were 11.52 mm and 11.22 mm, respectively, but those stocked at the density of 30 larva per liter of water were 7.55mm, which was the lowest. The survival rate of red marbled rockfish larva stocked at the density of 2.5 larva per liter of water was 52.0%, which was the best, but the lowest, 18.0% for the red marbled rockfish larva stocked at the density of 30 larva per liter. There was a trend toward decrease in survival rate of red marbled rockfish larva as their stocking density increased. The survival rates of red marbled rockfish larva fed rotifers at the density of 10 and 5 individuals per ml were high, 48.5% and 48.0%, and their total lengths were 11.92 and 11.89mm, respectively, which grew relatively fast. The survival rate and the total length of red marbled rockfish larva fed rotifers at the density of 30 individuals per ml were the lowest, which were 8.40mm and 21.5%, respectively. Also, red marbled rockfish larva fed rotifers at the density of more than 20 individuals per ml achieved poor survival and growth. These results indicated that the proper conditions for the mass production of larval red marbled rockfish were $23^{\circ}C$ of water temperature, fish larva stocking density of 5 to 10 larva per liter of water and rotifers density of 5 to 10 individuals per ml as live feed.

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Three New Species of Procloeon (Ephemeroptera: Baetidae) from Thailand

  • Tungpairojwong, Nisarat;Bae, Yeon Jae
    • Animal Systematics, Evolution and Diversity
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    • v.31 no.1
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    • pp.22-30
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    • 2015
  • Three baetid mayflies in the genus Procloeon Bengtsson are described from Thailand: P. narumonae, n. sp. (male and female adults and larva), P. rubeosternum, n. sp. (male and female adults, male and female subimagos, and larva), and P. siamensis, n. sp. (larva). Descriptions of known stages with line-drawings of diagnostic characters, material data, diagnoses, habitat data, and taxonomic remarks are provided.

Acute Toxicity of Nitrite, Ammonia and Hydrogen Sulfide for Early Developmental Stages of Fenneropenaeus chinensis (대하의 초기생활사에 있어 아질산, 암모니아 및 황화수소의 급성독성)

  • Ji, Jeong-Hun;Gang, Ju-Chan
    • Journal of fish pathology
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    • v.17 no.3
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    • pp.199-205
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    • 2004
  • Effects of nitrite, ammonia and hydrogen sulfide on survival of the early developmental stages of Fenneropenaeus chinensis were determined under continuous flow-through system. The 96hr-$LC_{50}$ values of mysis stage were 18.4 mg/L, 0.69 mg/L and 13.5 $\mu{g}/L$ for nitrite, ammonia and hydrogen sulfide, respectively; 28.3 mg/L, 1.23 mg/L and 20.7 $\mu{g}/L$ for post larva stage and 39.8 mg/L, 1.73 mg/L and 28.5 $\mu{g}/L$ for juvenile stage, respectively. The Fenneropenaeus chinensis sensitivity for the three pollutants was in the order of hydrogen sulfide>ammonia>nitrite. The mysis/post larva, mysis/juvenile and post larva/juvenile ratios of nitrite, ammonia and hydrogen sulfide toxicity were >1.5, >2.0 and <1.5 times, respectively, and mysis were found to be more sensitive to pollutants than juvenile in all cases.

Evaluation of Seawater Quality from Incheon Offshore Using Early Development Systems of A Sea Urchin (성게의 초기 발생계를 이용한 인천연안해수 수질평가)

  • Yu, Chun-Man
    • Journal of Environmental Health Sciences
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    • v.35 no.6
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    • pp.486-490
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    • 2009
  • In January 2009, the water quality of offshore around the Incheon coast was evaluated by bioassay using early development systems of a sea urchin species, Hemicentrotus pulcherrimus. The results of performing biological evaluations on seawater samples from total of thirteen sites, showed that the formation rates of normal pluteus larva varied from 18% to 71%. In site 5 the seawater sample led to an averaage formation rate of normal larva of 18%, the highest abnormal formation rate hindering the early embryo development of the experimental animal, while that of site 3 averaged 71%, the highest formation rate of normal larva. Seawater samples from site 1, 2, 4, 7, 9, 10, 11 and 12, resulted in average formation rates of normal larva from 33% to 56%, which indicates the developmental damage of early embryos is not severe. Seawater samples from site 5, 6, 8 and 13, resulted in average formation rates of normal larva from 18% to 21% which there was strong damage to the development of early embryos.

Culture Condition of Entomopathogenic Nematodes Using Galleria mellonella Larva (Galleria mellonella 유충을 이용한 곤충병원성 선충의 배양 조건)

  • 김도완;박선호
    • KSBB Journal
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    • v.13 no.1
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    • pp.31-37
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    • 1998
  • A simple method for the in vivo production of third-stage infective juveniles(IJs) of Steinernema glaseri was developed. Using Galleria mellonella larvae, only IJs can be rapidly generated inadequate quantities for field application. The nematode inoculation concentration and incubation temperature were critically important. The most effective temperature for infectivity of Steinernema glaseri IJs to Galleria mellonella larvae was 33$^\circ C$. However, the total number of menatodes harvested at 25$^\circ C$ about 66,000 IJs per larva was significantly greater than those at other temperatures. The optimal inoculation number of nematodes was 60 to 80 nematodes per host larva. The higher nematode inoculation concentration of 100 IJs per larva caused a rapid decrease in the total number of IJs harvested. As the inoculation medium pH increased, the number of IJs harvested increased and reached about 110,000 IJs per larva at pH 9.0. The pathogenicity of IJs decreased y increasing the salt concentration in the medium.

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Evaluation of Offshore Seawater Qualify using Gametes and Embryos of Starfishes (Asterina pectinifera) (불가사리 알을 이용한 연안해수의 수질 평가)

  • Yu Chun Man;Lee Jong Bin;Park Jong Cheon;Joo Hyun Soo
    • Korean Journal of Environmental Biology
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    • v.22 no.3
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    • pp.376-380
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    • 2004
  • In August 2003, the water quality of offshore waters along the Incheon coast of Korea was evaluated by biological evaluation using gametes, embryos and early development systems of a starfish species (Asterina pectinifera). As the result of performing biological evaluations on seawater samples from a total thirteen sites, the formation rate of normal larva was 16-68%. At seawater sample from site 5 and 13, formation rate of normal larva averaged 16%, the most abnormal rate hindering the early embryo development of the experimental animal, while that of site 3 averaged 68%, the highest formation rate of normal larva. At seawater sample from site 2, 4, 7, 9, 10, 11, 12, formation rate of normal larva averaged 33-54%, those which damage the development of early embryos slightly. At seawater sample from site 1, 5, 6, 8, 13, formation rate of normal larva averaged 16-28%, those which damage the development of early embryos strongly.

Fatty Acid Composition and Anti-inflammatory Effects of the Freeze Dried Tenebrio molitor Larva (동결건조 갈색거저리 유충의 지방산 조성과 항염증 효과)

  • Kang, Mi-Sook;Kim, Min Ju;Han, Jung-Soon;Kim, Ae-Jung
    • The Korean Journal of Food And Nutrition
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    • v.30 no.2
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    • pp.251-256
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    • 2017
  • This study aimed to assess fatty acid composition and anti-inflammatory effects, such as nitric oxide(NO) production, expression of $TNF-{\alpha}$ and interleukin-6(IL-6), of Tenebrio molitor larva using RAW 264.7 cells. The content of total fatty acid in Tenebrio molitor larva was 76.14%, which was composed of oleic acid(42.12%), linoleic acid(32.67%) etc. There was no cytotoxicity at a dose level of 0.1, 1.0, 10, and $100{\mu}g/mL$ of freeze dried Tenebrio molitor larva ethanol extract(FDTEtOH) on RAW 264.7 cells. FDTEtOH significantly decreased NO production in LPS(lipopolysaccharide)-stimulated RAW 264.7 cells in a dose-dependent manner. Also, FDTEtOH dose-dependently suppressed the expression of $TNF-{\alpha}$ and IL-6. Thus, these results showed that Tenebrio molitor larva has the potential to be used as an anti-inflammatory food to improve immunity.

Ophthalmomyiasis Caused by a Phormia sp. (Diptera: Calliphoridae) Larva in an Enucleated Patient

  • Kim, Jae-Soo;Kim, Jong-Wan;Lee, Hye-Jung;Lee, In-Yong;Oh, Sang-Ah;Seo, Min
    • The Korean Journal of Parasitology
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    • v.49 no.2
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    • pp.173-175
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    • 2011
  • Ophthalmomyiasis rarely occurs worldwide, and has not been reported in Korea. We present here a case of ophthalmomyiasis caused by Phormia sp. fly larva in an enucleated eye of a patient. In June 2010, a 50-year-old man was admitted to Dankook University Hospital for surgical excision of a malignant melanoma located in the right auricular area. He had a clinical history of enucleation of his right eye due to squamous cell carcinoma 5 years ago. During hospitalization, foreign body sensation developed in his right eye, and close examination revealed a fly larva inside the eye, which was evacuated. The larva was proved to be Phormia sp. based on the morphology of the posterior spiracle. Subsequently, no larva was found, and the postoperative course was uneventful without any complaints of further myiasis. This is the first case of ophthalmomyiasis among the literature in Korea, and also the first myiasis case caused by Phormia sp. in Korea.

Fundamental Study about Bottom-Clinging Rate in Free Floating Larva by Infiltration Flow in Tidal (조석의 침투류에 의한 패류 유생의 착저 (着底) 효과에 관한 기초적 연구)

  • Back, Sang-Ho;Park, Kwang-Jae;Park, Young-Je;Cheon, Jun-Je;Cho, Kee-Chae;Kim, Yi-Un
    • The Korean Journal of Malacology
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    • v.25 no.2
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    • pp.97-103
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    • 2009
  • It is a research that free floating larva of Ruditapes philippinarum in compliance with infiltration flow of tidal accumulation and bottom-clinging system in high density also, It is a precondition this for the continuous augmentation of Ruditapes philippinarum resources. So in flow field of tidal the free floating larva produced the infiltration water tank that can possibly bottom-clinging and experiment the water tank which uses free floating larva, It evaluates that the acceleration effect of free floating larva by infiltration in objective. As a result, 1) The experiment on free floating larva's bottom sediment grain diameter came to be high recording as bottom-clinging rate at the static water field and even biologically it selects bottom-clinging substrate it will be able to confirm. 2) About occurrence of infiltration flow field is in case of that: the drift of a current 10cm/s, bottom sediment grain 1.21mm infiltration flow 0.3cm/s increase of 3~5 times was confirmed. 3) From free floating larva of Ruditapes philippinarum the choice of bottom sediment grain diameter depends in the biological factor and form the flow field the bottom-clinging acceleration effect was controled over physical stable condition. 4) In case of density of Ruditapes philippinarum free floating larva of sea area, bottom sediment grain diameter, flow condition which are very cleanly in the research that possibly could conjecture the free floating larva's bottom-clinging rate.

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Biochemical Changes in the Hemolymph of the Larvae of Thecodiplosis japonensis Uchi. et Inouye (솔잎혹파리 유충 체액의 생화학적 변화)

  • Lee Kyung-Ro;Lee Jong-Jin
    • Korean journal of applied entomology
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    • v.15 no.4
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    • pp.169-178
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    • 1976
  • The concentration of amino acids, total nitrogen, trehalose, lipids and the activities of respiratory, acid$\cdot$alkaline phosphatase, glutamic oxalozcetic transaminase and glutamic pyruvic transaminase during larval stage in Pine leaf gall midge, Thecodiplosis janensis Uchi. et Inouye were measured using Paper chromatographic method, micro-Kjeldahl method, Thin layer chromatographic method, Warburg's manometric method, Bessey-Lowry method and Reitman-Frankel method, respectively. Healthy specimens )yore chosen as samples of each larval stages; alrva in gall and larva in soil. Amino acids present in the alcoholic extracts were alanine, glutamic acid, glycine, histidine, methionine, proline, threonine, tryptophan and valine. The total nitrogen concentration reached to 31.348mg/g during the larva in gall and the larval stage in soil of the value was decreased to 29.027mg/g. The hemolymph sugar, trehalose value for larva in soil was about two times of the value for larva in gall. Total lipid, phospholipid,monoacylglycerol, triacylglycerol, sterol, free fatty acid and ester cholesterol were identified at larval stages in gall and soil. Triacylglycerol concentration reached high level in contrast with other lipid contents during larvae in gall and larva in soil. Free fatty acid, sterol except decreased lipids during larval stage in soil. Endogenous respiration, succinate of respiratory activities decreased at larval stage in soil compare with larva in gall. The activities of acid phosphatase decreased larval stage in soil but the activities of alkaline phosphatase increased remarkably. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase reached high level of the larva in gall.

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