• Title, Summary, Keyword: Korean native tea leaves

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Volatile Components of Korean Native Tea Leaves (Camellia sinensis O. Kuntze) (한국(韓國) 자생차엽(自生茶葉)의 향기성분(香氣成分))

  • Park, Jang-Hyun
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.3
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    • pp.295-300
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    • 1997
  • Flavor components of natively grown tea plant(Camellia sinensis O. kuntze) in Korea, collected from 12 locations, were analyzed by gas chromatograph and mass spectrometer. Seventy to eighty flavor components in tea leaves were separated by GC. Total 52 flavor components were identified by comparing gas chromatograhp retention time and mass spectral date. They were classified as 19 alcohols, 5 aldehydes, 2 hydrocarbons, 6 ketones, 4 esters, 3 lactones, 2 acids, 3 phenols, 4 pyrazines, and 4 nitrogenous compounds. Major compounds identified were geraniol, linalool oxide, 1-hexanol and ethanol.

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First Report on Gray Blight of Tea Plant Caused by Pestalotiopsis theae in Korea

  • Shin, Gil-Ho;Park, Hyoung-Koog;Hur, Jae-Seoun;Koh, Young-Jin
    • The Plant Pathology Journal
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    • v.15 no.5
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    • pp.308-310
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    • 1999
  • A fungus associated with gray blight on tea plant (Camellia sinensis) was identifed as Pestalotiopsis theae based on the mycological characteristics. Mycelial growth on potato dextrose agar and size and shape of conidia of P. theae were similar to those of P. longiseta, but P. theae was different from P. longiseta in the color of three median cells and the number of apical appendages. Artificial inoculation of conidial suspension or mycelial mats on the wounded leaves and shoots of healthy plants induced the same disease, respectively. The Korean native variety was relatively. The Korean native variety was relatively more resistat to P. theae than a Japanese variety‘Yabukita’which has bee recently introduced and planted in large areas of southern parts of Korea. Here, we report the report the first record of gary blight caused by P. theae on tea plant in Korea.

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Studies on Growth Enviromental and Inorganic Components of Korean Native Tea Plants(Camellia sinensis O. kuntze) (한국(韓國) 자생차(自生茶)의 생육지 토양과 엽중 무기성분 함량)

  • Park, Jang-Hyun;Kim, Kwang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.1
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    • pp.25-32
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    • 1998
  • To characterize the growth environment, inorganic composition and morphological chracteristics of leaves of Korean tea plant, soil and tea leaf samples were collected from 15 locations and analyzed. The chemical characteristics of soils were in range of pH 4.09~6.15, OM 23.9~72.6g/kg, available phosphate less than 300mg/kg, K $0.8{\sim}2.5cmol^+/kg$, Na $tr{\sim}0.17cmol^+/kg$, Ca $1.0{\sim}6.2cmol^+/kg$, and Mg $0.3{\sim}2.1cmol^+/kg$. The contents of Ni. Cr, Zn. Cu, Pb, and Cd were at the level less than natural content in upland soil. Most of the sample soils were sandy loamy and loamy texture. The native tea plants were mainly grown in bamboo thicket or in forest. The leaf sizes of tea plants were $6.85{\pm}1.75{\times}2.6{\pm}0.5cm$, lateral vein number $14.2{\pm}2.7$, and crenated number $58.5{\pm}11.2$, and the leaf color was thin to dark green. The contents of $NH_4{^+}$, $Na^+$, $K^+$, $Mg^{2+}$ and $Ca^{2+}$ in tea leaves were in range of 30.5~47.7, 45.5~164.5, 16,998~25,431, 1.590~2,392 and 1,085~1,958mg/kg, respectively. The contents of $F^-$, $Cl^-$, $NO_3{^-}$, $PO_4{^{3-}}$ and $SO_4{^{2-}}$ were in range of 21.2~63.2, 126.4~257.9, 108.5~185.9, 1,270~1.819, and $954{\sim}1,670mg/kg$, respectively. The leaf size of native tea plant grown widlly in Shunchun Changchun-ri, Hwasun Ssangbongsa, Kuryoi Chonunsa, Bosong Daewonsa and Namhae Boriam was as large as those of Yabukita. Japan cultiver, grown at Kangjin Jangwon tea field.

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Chemical Components of Korean Native Tea Plants (한국 자생차의 몇가지 화학성분 비교분석)

  • Park, Jang-Hyun;Kim, Kwang-Sik;Kim, Sun-Woo;Choi, Hyoung-Kog;Kim, Sang-Chol
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.3
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    • pp.217-224
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    • 1997
  • The significant chemical components estimating the quality of green tea were compared and analyzed in the shoots of Korean native tea plants. The results are summarized as follows. The contents of total nitrogen in tea leaves were in range of $3.59{\sim}4.89%$, and the tea plants grown wildly in Bosong Daewonsa, Hwasun Ssangbongsa, Shunchun Changchun-ri, Hadong Ssanggyesa. and the cultivated tea plants in Kangjin Jangwon Sanup had higher contents of total nitrogen. The contents of tannin ranged from 12.5 to 18.3%. The contents of tannin of Kuryoi Chonunsa (18.3%), Kangjin Baekryonsa (16.7%) and Naju Bulhoisa (16.4%) were higher than those of Yangkwang Chonma-ri (12.5%), Hampyong Yongam-ri (12.7%) and Yosu Udu-ri (12.8%). The contents of caffeine were in range of $2.21{\sim}3.11%$. The contents of caffeine of Kwangju Shamae Dawon (3.11%), Kangjin Jangwon Sanup (2.94%) and Shunchun Changchun-ri(2.87%) were higher than those of Tamyang Yanggak-ri(2.21%), Yosu Udu-ri(2.23%) and Kuryoi Hwaomsa (2.23%). The contents of vit. C were in range of $167.9{\sim}223.5mg/100g$. The contents of vit. C of Changsong Oshan-ri (167.9mg/100g), Shunchun Shongkwangsa (185.6mg/100g) and Yongkwang Chonma-ri (185.8mg/100g) were lower than those of Kwangju Shamae Dawon (291.5mg/100g), Kangjin Jangwon Sanup (271.8mg/100g) and Shunchun Changchun-ri (269.5mg/100g). The contents of chlorophyll were in range of $187.4{\sim}332.7mg/100g$, and the mean contents were in range of 242.0mg/100g. The contents of total amino acids were in range of $1,888{\sim}2,500mg/100g$. The content of total amino acid at Kangjin Jangwon Sanup was the highest as 2,500mg/100g, and that of Shunchon Shongkwangsa was the lowest as 1,888mg/100g. Results of this study suggest that the native tea plants naturally grown at Bosong Daewonsa, Hwasun Ssangbongsa, Shunchun Changchun-ri, Hadong Ssanggyesa, and the cultivated tea plants at Kangjin Jangwon Sanup show excellent quality in terms of abundance of total nitrogen and total amino acids, and less contents of tannin.

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Antioxidant Activity of Green Tea Extracts toward Human Low Density Lipoprotein (사람의 Low Density Lipoprotein에 대한 녹차의 항산화 활성)

  • Park, Chun-Ok;Jin, Seung-Heun;Ryu, Beung-Ho
    • Korean Journal of Food Science and Technology
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    • v.28 no.5
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    • pp.850-858
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    • 1996
  • Green tea leaves 12.5 g were extracted twice with 500 ml boiling water. The green tea extract (GTE) contained 4.67 mg solid. The GTE contained polyphenols sush as 54.12% (-) epicatechin gallate, 26.21% (-) epicatechin, 10.71% epicatechin gallate, 7.09% (-) epicatechin and 1.85% catechin. The GTE inhibited the copper-catalyzed oxidation of human LDL at the concentrations of 50 and $100\;{\mu}g/ml$ GTE in the presence of $5\;{\mu}M$ $CuSO_{4}$. The electrophoretic mobility of the LDL oxidized in the presence of $5\;{\mu}M\;CuSO_{4}$ was higher than that of the native LDL. The GTE also inhibited LDL oxidation induced by J774, human monocyte-derived macrophages and vascular endotherial cells. The LDL modified by copper or cells was inhibited by human macrophages at a much greater rate than native LDL in the presence of GTE. The GTE was found to be a potent inhibitor of modification of LDL. GTE inhibited the uptake of cell-modified $^(125)I-labelled$ LDL by macrophages. The formation of conjugated dienes was strongly inhibited in the presence of 50 or $100\;{\mu}g/ml$ GTE.

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Major Components of Teas Manufactured with Leaf and Flower of Korean Native Camellia japonica L. (국내 자생 동백나무의 잎과 꽃으로 만든 엽차와 화차의 주요성분)

  • Cha, Young-Ju;Lee, Jang-Won;Kim, Ju-Hee;Park, Min-Hee;Lee, Sook-Young
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.3
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    • pp.183-190
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    • 2004
  • The major compositions of leaf tea and flower tea were investigated to develope as a new functional tea using Korean native Camellia japonica L. Most of leaf teas, except flower tea, were considered as good materials with basic conditions for tea manufacture because water content was below 6%. Crude protein was the greatest component in roasted young leaf tea (RYLT), crude fats in roasted mature leaf tea (RMLT) and ashes in fermented young leaf tea (FYLT). Caffein were present as the highest amount (5.18%) in steamed mature leaf tea (SMLT), showing less amount than green tea. Catechin were contained as the highest amount in all kinds of teas, especially FYLT was the highest (9.57%). Tannin, which highly related with tea quality including astringent taste, color and perfume, were present as the highest amount in FYLT. Vitamin C was highly detected in the tea from flowers (22.7 mg/l00 g) rather than in the tea from leaves. The content of theanine were found in flower tea by 1,074 mg/l00 g, and had about twofold of FYLT and RYLT. Among free amino acids, glutamic acid and aspartic acid were higher detected in SMLT and RMLT while asparagine was present as higher amounts in RYLT and FYLT, expecting these components can improve tea taste. Nucleic acids and their derivatives including GMP, hypoxanthine and AMP were detected as the higher amounts by 7.86, 8.57, and $12.67\;{\mu}mol/g$, respectively, however IMP content was even reduced by all manufacturing processes. In all kinds of tea, sugars such as glucose, fructose, sucrose and maltose were detected, specially glucose and fructose were found as highest amount in RFT by 65.5 and 59.6 nmol/0.1 mg, respectively.

The Chemical Composition of Persimmon (Diospyros kaki, Thumb) Leaf Tea (감 (Diospyros kaki, Thumb) 잎차의 화학 성분)

  • 성낙주;정선영;이수정;조종수;강신권
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.5
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    • pp.720-726
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    • 1995
  • Chemical components relevant to the characteristic taste of the Korean native persimmon(Diospyros kaki, Thumb) leaf tea were analyzed. Samples were processed by using three different methods ; SHT(steamed and then hot-air dried), DHT(dried in the shade, steamed and then hot-air dried) and RHT(roasted and then hot-air dried). The components analyzed were general compositions of dried perisimmon leaves and extracted solution. The composition of moisture, ash, crude lipid and total nitrogen did not show significant variation among different processing methods of the persimmonleaf tea. The contents of caffeine, tannin and vitamin C in persimmon leaf tea were in the range of $178.4~209.8{\mu}mol/g$, 29.1~38.5mg% and 325.3~2084.7mg%, respectively. The vitamin C content was significantly higher in the RHT than other treatments. The contents of caffeine, tannin and vitamin C in the tea extracted solution were in the range of $101.5~130.1{\mu}mol/g$, 15.4~25.9mg% and 111.0~1274.3mg%, respectively. The vitamine C in the tea solution was the highest in the RHT treatment and 61.1% of vitamin C in the leaf tea was extracted out in these processing methods. The major amino acids contained in the leaf tea were in decreasing order glutamic acid, aspartic acid, leucine and phenylalanine, these four amino acids consisting 38.9~39.8% of the total amino acid contained in the persimmon leaf tea. The major amino acids contained in the tea solution were glutamic acid, proline, histidine and arginine. Six kinds of 5'-nucleotides, CMP, AMP, UMP, IMP, GMP and hypoxanthine were detected and CMP was the most abundant component in fresh leaf, leaf tea and tea solution. The second highest 5'-nucleotides in both leaf tea and tea solutions were GMP, AMP and UMP in all processing method. The highest free sugar contained in the fresh leaf tea and tea solution was sucrose.

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Cytotoxicity and Chemosensitizing Effect of Camellia(Camellia japonica) Tea Extracts (동백엽차와 화차의 세포독성 및 다제내성 극복효과)

  • 황은주;차영주;박민희;이장원;이숙영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.3
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    • pp.487-493
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    • 2004
  • This study has been undertaken to increase availability of native camellia in Jeonnam as a medicinal resource and to isolate the effective components from them. Fresh leaf and flower of camellia, single camellia tea and camellia tea mixed with green tea, herbs were screened for cytotoxicity on MCF -7 (human breast adenocarcinoma pleual effusion), Calu-6 (human pulmonary carcinoma), SNU-601 (human gastric carcinoma) cells. Also their multidrug-resistance reversing activity were evaluated using drug sensitive AML-2/WT and multidrug-resistant AML-2/D100 cells. Among the camellia extracts, young leaf and camellia tea mixed with green tea had strong growth inhibitory effects in below 100 $\mu\textrm{g}$/mL against human cancer cells. In result, young leaf showed the strongest inhibitory effects on MCF -7 ($IC_{50}$/ = 100 $\mu\textrm{g}$/mL ↑), Calu-6 ($IC_{50}$/ = 79 $\mu\textrm{g}$/mL), and SNU -601 ($IC_{50}$/ = 39 $\mu\textrm{g}$/mL), and AML-2/WT ($IC_{50}$/ = 64 $\mu\textrm{g}$/mL). Chemosensitizing effect was the extracts of mature leaf ($IC_{50}$/ = 97 $\mu\textrm{g}$/mL, RF=3.0), roasted tea ($IC_{50}$/ = 76 $\mu\textrm{g}$/mL, RF = 2.6 ↑) and steam tea ($IC_{50}$/ = 70 $\mu\textrm{g}$/mL, RF=2.8 ↑) strongly potentiate vincristine cytotoxicity in AML-2/D100 cells. But their cytotoxicities to both sensitive AML-2/WT and resistant AML-2/D100 cells were in the same order of magnitude. This results indicate that crude extracts of camellia mature leaves would contain some principles which have chemosensitizing activity.

Neuronal Protection by Rooibos (Aspalathus linearis) Tea Infusions in a Hypoxic Model of Cultured Rat Cortical Neurons (흰쥐 대뇌세포배양의 저산소증모델에서 루이보스차 침제에 의한 신경세포 보호작용)

  • Moon, Il-Soo;Ko, Bok-Hyun
    • Journal of Life Science
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    • v.14 no.2
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    • pp.291-295
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    • 2004
  • Rooibos (Aspalathus linearis) (RB) is a leguminous shrub native to the mountainous areas of the northwestern Cape Province in South Africa. RB tea infusions are the fermentation products of its leaves and fine sterns, and known to have a high antioxidative activity due to the presence of flavonoids and phenolic acids. We investigated the effects of RB tea on the alleviation of oxidative stress on cultured rat cortical neurons in a hypoxic model. Measurement of lactate dehydrogenase (LDH) released into culture media revealed that RB increased cell viabilities in both normoxia (6-18%) and hypoxia (2-24%) dose-dependently (10-100 $\mu\textrm{g}$/ml) on 16 days in vitro (3 days after treatment). Visualization of cell morphology by expression of GFP-Hsc70 fusion protein showed that RB (50 $\mu\textrm{g}$/ml) reduced the average vacuolated soma from 55.4$\pm$4.59% (no RB addition) to 40.9$\pm$6.3% (RB addition) on 5 days after hypoxia. Our results proves efficacy of RB in the neuroprotection of hypoxic neurons and extend application for RB into the prevention and/or treatment of neuronal damages.