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Isolation and Characterization of a Type II Peroxiredoxin Gene from Panax ginseng C. A. Meyer

  • Kim, Yu-Jin;Lee, Jung-Hye;Lee, Ok-Ran;Shim, Ju-Sun;Jung, Seok-Kyu;Son, Na-Ri;Kim, Ju-Han;Kim, Se-Young;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.34 no.4
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    • pp.296-303
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    • 2010
  • A peroxiredoxin cDNA (PgPrx) was isolated and characterized from the leaves of Panax ginseng. The cDNA is 716 nucleotides long and has an open reading frame of 489 base pairs with a deduced amino acid sequence of 162 residues. The calculated molecular mass of the mature protein is approximately 17.4 kDa with a predicted isoelectric point of 5.37. A GenBank BlastX search revealed that the deduced amino acid sequence of PgPrx shares a high degree homology with type II peroxiredoxin (Prx) proteins in other plants. The PgPrx gene was highly expressed in leaves, and expressed at a low level in the stem. To analyze the gene expression of PgPrx in response to various abiotic stresses, we utilized real-time quantitative RT-PCR. Our results reveal that PgPrx expression is induced by ultraviolet irradiation, low temperature, and salt. The induction of PgPrx in response to abiotic stimuli suggests that ginseng Prx may function to protect the host against environmental stresses.

Isolation of Sesquiterpene Synthase Homolog from Panax ginseng C.A. Meyer

  • Khorolragchaa, Altanzul;Parvin, Shohana;Shim, Ju-Sun;Kim, Yu-Jin;Lee, Ok-Ran;In, Jun-Gyo;Kim, Yeon-Ju;Kim, Se-Young;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.34 no.1
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    • pp.17-22
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    • 2010
  • Sesquiterpenes are found naturally in plants and insects as defensive agents or pheromones. They are produced in the cytosolic acetate/mevalonate pathway for isoprenoid biosynthesis. The inducible sesquiterpene synthases (STS), which are responsible for the transformation of the precursor farnesyl diphosphate, appear to generate very few olefinic products that are converted to biologically active metabolites. In this study, we isolated the STS gene from Panax ginseng C.A. Meyer, designated PgSTS, and investigated the correlation between its expression and various abiotic stresses using real-time PCR. PgSTS cDNA was observed to be 1,883 nucleotides long with an open reading frame of 1,707 bp, encoding a protein of 568 amino acids. The molecular mass of the mature protein was determined to be 65.5 kDa, with a predicted isoelectric point of 5.98. A GenBank BlastX search revealed the deduced amino acid sequence of PgSTS to be homologous to STS from other plants, with the highest similarity to an STS from Lycopersicon hirsutum (55% identity, 51% similarity). Real-time PCR analysis showed that different abiotic stresses triggered significant induction of PgSTS expression at different time points.

Characterization of a Korean Isolate of Dasheen mosaic virus Isolated from Taro (Colocasia esculenta Schott) in Korea

  • Kim, Min-Kyu;Kwon, Soon-Bae;Yoon, Ju-Yeon;Ryu, Ki-Hyun;Heo, Su-Jeong;Hong, Jeong-Ki;Kim, Kyung-Hee;Park, Jang-Kyung
    • The Plant Pathology Journal
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    • v.20 no.2
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    • pp.135-141
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    • 2004
  • A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feather-ing symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.

Design and Operation Characteristics of 2.4MJ Pulse Power System for Electrothermal-Chemical(ETC) Propulsion(I) (전열화학추진용 2.4MJ 펄스파워전원의 설계와 동작특성(I))

  • Jin, Y.S.;Lee, H.S.;Kim, J.S.;Cho, J.H.;Lim, G.H.;Kim, J.S.;Chu, J.H.;Jung, J.W.;Hwang, D.W.
    • Proceedings of the KIEE Conference
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    • pp.1868-1870
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    • 2000
  • As a drive for an ETC (Electro-thermal Chemical) launcher, a large pulse power system of a 2.4MJ energy storage was designed, constructed and tested. The overall power system consists of eight capacitive 300kJ energy storage banks. In this paper we describe the design features, setup and operation test result of the 300kJ pulsed power module. Each capacitor bank of the 300kJ module consists of six 22kV 50kJ capacitors. A triggered vacuum switch (TVS-43) was adopted as the main pulse switch. Crowbar diode circuits, variable multi-tap inductors and energy dumping systems are connected to each high power capacitor bank via bus-bars and coaxial cables. A parallel crowbar diode stack is fabricated in coaxial structure with two series 13.5kV, 60kA avalanche diodes. The main design parameters of the 300kJ module are a maximum current of 180kA and a pulse width of 0.5 - 3ms. The electrical performances of each component and current output variations into resistive loads have been investigated.

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Potential for Development of Bank Filtrate in the Nakdong River Basin (낙동강 유역의 강변여과수 개발 가능성)

  • 전흥배;김상일
    • Journal of the Korean Professional Engineers Association
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    • v.30 no.4
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    • pp.99-116
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    • 1997
  • In order to obtain safe drinking water, free from surface contamination, a study to determine the potential for developing a bank-filtrate system in the Iryong and Yongsan, Nakdong River Basin, Korea was conducted. The main type of aquifer In the study area is alluvial, consisting mostly of sand and gravel. The hydraulic conductivity(k) of the Iryong and Yongsan test areas were 8.63${\times}$10$^-2$cm/s and 9.90${\times}$10$^-2$cm/s, respectively, indicating that these areas are satisfactory for bank filtrate production. Pilot plants(IRPL and YSPL) were set up In Iryong and Yongsan to monitor the change in the quality of bank-filtered water and to determine the effect pumping had on the surrounding hydrogeologic system. The pilot plants operated continuously for about two months and the data obtained were used to validate the groundwater flow model. Computer simulations were conducted to predict the effects of producing bank filtrate using MODFLOW. MODPATH was also linked with the flow model to analyze particle tracking. According to the results of the model simulations and the hydrogeologlc study, long-term pumping, the minimization of drawdown and the availability of uncontaminated sell and groundwater conditions for the catchment area were all Important factors for successful bank-titrate system development.

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Design of M-Channel IIR Uniform DFT Filter Banks Using Recursive Digital Filters

  • Dehghani, M.J.;Aravind, R.;Prabhu, K.M.M.
    • ETRI Journal
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    • v.25 no.5
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    • pp.345-355
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    • 2003
  • In this paper, we propose a method for designing a class of M-channel, causal, stable, perfect reconstruction, infinite impulse response (IIR), and parallel uniform discrete Fourier transform (DFT) filter banks. It is based on a previously proposed structure by Martinez et al. [1] for IIR digital filter design for sampling rate reduction. The proposed filter bank has a modular structure and is therefore very well suited for VLSI implementation. Moreover, the current structure is more efficient in terms of computational complexity than the most general IIR DFT filter bank, and this results in a reduced computational complexity by more than 50% in both the critically sampled and oversampled cases. In the polyphase oversampled DFT filter bank case, we get flexible stop-band attenuation, which is also taken care of in the proposed algorithm.

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Performance Evaluation of SME Banking in Bangladesh using Stochastic Frontier Analysis

  • Hossain, M.K.;Hossain, M.A.;Baten, M.A.
    • Asia-Pacific Journal of Business
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    • v.7 no.1
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    • pp.31-42
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    • 2016
  • Small and Medium Enterprises (SMEs) are suitable to provide employment with lower investment in densely populated countries like Bangladesh. A stochastic frontier model is used to evaluate performance of SME Banking of the commercial banks in Bangladesh. Input (Total Deposit, Cost of Fund and Salary Expenditure) and output (Finance to SME) data are collected on 45 banks which are dealt with SME for 13 quarters from $1^{st}$quarter of 2010 to $2^{nd}$quarter of 2013. Average performance of the SME banking is 0.716 in Bangladesh. That is, banks have opportunity to increase 30% performance in SME banking from the same inputs. Bangladesh Development Bank has lowest performance (0.540) while Eastern bank has the highest performance (0.753). Highest (0.743) and lowest (0.662) performance is observed during the second quarter of 2013 and fourth quarter of 2010 respectively. Inefficient Bank might be benefited by following the rules of efficient banks.

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Iron and Manganese Removal through Well Development at River Bank Filtration Site (강변여과수 개발지역 우물 개량시 철망간 저감 효과)

  • Kim, Gyoo-Bum;Kim, Byung-Woo;Shin, Seon-Ho;Park, Joon-Hyeong
    • The Journal of Engineering Geology
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    • v.19 no.3
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    • pp.389-400
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    • 2009
  • Iron and manganese contents are usually over the limit of drinking water standard (0.3 mg/L) in unconsolidated aquifer of river bank filtration site in Nakdong river. Surge block and air surging techniques used in this study are useful tools to remove the slime within gravels and pebbles, to increase permeability of aquifer, to provide oxygen into aquifer, and to discharge iron and manganese oxides from a well. Surging activity brought about $5{\sim}8$ and $5{\sim}9$ times decreases in $Fe_{(total)}$ and $Fe^{2+}$ contents, and also 10 times decrease in $Mn^{2+}$ contents compared to non-surging condition, respectively. Additionally, iron oxide and manganese oxide increased up $1{\sim}1.2$ times after surging. This result shows that air injection into the aquifer can help iron and manganese content decreased and in-situ treatment technology needs to be introduced in river bank filtration project in South Korea.

Isolation and Identification of Lactobacillus kimchicus sp.nov and Bioconversion of Ginseng Saponin

  • Liang, Zhi-Qi;Kim, Ho-Bin;Kim, Yeon-Ju;Wang, Hong-Tao;Yang, Deok-Chun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • pp.15-15
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    • 2010
  • Ginseng contained many different kinds of saponin which was the most valuable for people, but its yield cannot satisfy the demand using traditional extract methods. Enzyme transformation is a conformable and highly performed method which was fit for today. A ${\beta}$-glucosidase producing bacterium ($DCY51^T$) was isolated from Korean fermented-vegetable food kimchi. The 16S rRNA gene sequence analysis revealed that the strain $DCY51^T$ belongs to the genus Lactobacillus. The highest sequence similarity was found with Lactobacillus paracollinoides LMG $22473^T$ and Lactobacillus collinoides LMG $9194^T$ with levels of 16S rDNA similarity of 97.4% and 97.3%, respectively. Based on the above results the strain $DCY51^T$ placed in the genus Lactobacillus and proposed a new species, Lactobacillus kimchicus sp. nov. $DCY51^T$ (= KCTC $12976^T$ = JCM $15530^T$). It was culture solution reacted with Red Ginseng extract and $Rb_1$, respectively. The medium of bacteria was the liquid of MRS, the temperatures of growing and reacting between bacteria liquid and saponin were samely $37^{\circ}C$, there spective reacting time were 12 hours and 48 hours. Thus we got different saponins, and TLC and HPLC analysis showed that: enzyme respectively reacted with $Rb_1$ and Red Ginseng extract got the transformed saponin, respectively. The polarity position in TLC was a little higher than Rd; and the polarity position was the same as that of Compound K's, the saponin obtained from HPLC and other experimental results was not Compound K. The constitution of its saponin was hoped to be further confirmed.

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