• Title, Summary, Keyword: Jasmonate

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Effects of Jasmonic acid and Methyl jasmonate on the Production of Ginsenosides in the Hairy Roots of Korean Ginseng (Panax ginseng C.A. Meyer) (고려인삼(Panax Ginseng C.A. Meyer) 모상근으로부터 Ginsenosides 생산에 미치는 Jasmonic acid와 Methyl jasmonate의 영향)

  • 박효진;오승용;최경화;맹성주;윤의수;양덕춘
    • Journal of Ginseng Research
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    • v.24 no.2
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    • pp.74-78
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    • 2000
  • To elucidate the effects of jasmonic acid and methyl jasmonate on the production of ginsenosides and growth, ginseng hairy root KGHR-8 clone was cultured on the 1/2 MS medium without growth regulators, which was supplemented with of various concentrations jasmonic acid and methyl jasmonate and culture period. The highest growth rate was obtained when 1$\mu\textrm{m}$ jasmonic acid and methyl jasmorlate were treated. However, the growth was inhibited at more than 30$\mu\textrm{m}$ of concentration. Treatment with high concen Dation of jasmonic acid (10$\mu\textrm{m}$) and methyl jasmonate (50$\mu\textrm{m}$) increased the contents and productivity of ginsenosides reversion of the growth inhibition. The highest contents and productivity of ginsenosides were appeared at 4 weeks after onset of the treatment of jasmonic acid and at 3 weeks in the case of methyl jasmonate.

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Effect of Methyl Jasmonate on the Root Growth and the Eleutheroside Accumulation in the Adventitious Root Culture of Eleutherococcus senticosus (가시오갈피나무 부정근(不定根) 배양(培養)에서 부정근(不定根)의 생장(生長)과 Eleutheroside류(類)의 생산(生産)에 미치는 Methyl jasmonate 처리(處理)의 영향)

  • Ahn, Jin-Kwon;Lee, Wi-Young;Park, Eung-Jun
    • Journal of Korean Society of Forest Science
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    • v.99 no.3
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    • pp.331-336
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    • 2010
  • This study was carried out to investigate the dose-dependent effect of methyl jasmonate on both the adventitious root growth and the accumulation of various eleutherosides in the bioreactor culture of Eleutherococcus senticosus adventitious roots. The highest biomass production (5.4 g DW/L) was observed in the absence of methyl jasmonate and the root growth was significantly decreased by increasing the methyl jasmonate concentration. However, methyl jasmonate stimulated the production of both eleutheroside B, E and $E_1$. The highest level of eleutheroside B (359.9 ${\mu}g$/g DW) was obtained at 40 ${\mu}M$ of methyl jasmonate, while eleutheroside E and $E_1$ was accumulated at the highest level by the addition of 10 ${\mu}M$ of methyl jasmonate. Total eleutheroside was increased up to 3818.1 ${\mu}g$ per liter when 10 ${\mu}M$ of methyl jasmonate was applied. In addition, when the adventitious roots were cultured with 20 ${\mu}M$ of methyl jasmonate, the highest levels of eleutheroside B, E and $E_1$ were observed at the 12th, 3th and 9th days of culture, respectively.

The Arabidopsis AtLEC Gene Encoding a Lectin-like Protein Is Up-Regulated by Multiple Stimuli Including Developmental Signal, Wounding, Jasmonate, Ethylene, and Chitin Elicitor

  • Lyou, Seoung Hyun;Park, Hyon Jin;Jung, Choonkyun;Sohn, Hwang Bae;Lee, Garam;Kim, Chung Ho;Kim, Minkyun;Choi, Yang Do;Cheong, Jong-Joo
    • Molecules and Cells
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    • v.27 no.1
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    • pp.75-81
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    • 2009
  • The Arabidopsis gene AtLEC (At3g15356) gene encodes a putative 30-kDa protein with a legume lectin-like domain. Likely to classic legume lectin family of genes, AtLEC is expressed in rosette leaves, primary inflorescences, and roots, as observed in Northern blot analysis. The accumulation of AtLEC transcript is induced very rapidly, within 30 min, by chitin, a fungal wall-derived oligosaccharide elictor of the plant defense response. Transgenic Arabidopsis carrying an AtLEC promoter-driven ${\beta}$-glucuronidase (GUS) construct exhibited GUS activity in the leaf veins, secondary inflorescences, carpel heads, and silique receptacles, in which no expression could be seen in Northern blot analysis. This observation suggests that AtLEC expression is induced transiently and locally during developmental processes in the absence of an external signal such as chitin. In addition, mechanically wounded sites showed strong GUS activity, indicating that the AtLEC promoter responds to jasmonate. Indeed, methyl jasmonate and ethylene exposure induced AtLEC expression within 3-6 h. Thus, the gene appears to play a role in the jasmonate-/ethylene-responsive, in addition to the chitin-elicited, defense responses. However, chitin-induced AtLEC expression was also observed in jasmonate-insensitive (coi1) and ethylene-insensitive (etr1-1) Arabidopsis mutants. Thus, it appears that chitin promotes AtLEC expression via a jasmonate- and/or ethylene-independent pathway.

Re-Elicitation with Methyl Jasmonate in Eschscholtzia californica Cell Suspension Cultures

  • Byun, Sang-Yo
    • Journal of Microbiology and Biotechnology
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    • v.10 no.1
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    • pp.107-110
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    • 2000
  • Elicited cells with methyl jasmonate continued to produce benzophenanthridine alkaloids throughout medium changes in suspension cultures of Eschscholtzia californica. Large increases in alkaloid production were observed by re-elitations with medium changes. The total alkaloid production increased during the successive elicitation steps reaching a maximum level on the 4th elicitation. The highest total alkaloid produced was 250 mg/I, which was 20fold higher than that of the single elicitation and 4-fold higher than that of the normal culture without elicitation. The large increases in alkaloid production in successive re-elicitations with medium changes are believed to be caused by the accumulation of the signal transduction compound, jasmonate.

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Efficient Elicitation of Ginsenoside Biosynthesis in Cell Cultures of Panax notoginseng by Using Self-chemically-synthesized Jasmonates

  • Wang Wei;Zhao Zhen-Jiang;Xu Yufang;Qian Xu hong;Zhong Jian-Jiang
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.2
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    • pp.162-165
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    • 2005
  • A series of fluorine and hydroxyl containing jasmonate derivatives, which were chemically synthesized in our institute, were investigated for their effects on the biosynthesis and heterogeneity of ginsenosides in suspension cultures of Panax notoginseng cells. Com-pared to the control (without addition of elicitors), $100{\mu}M$ of each of the jasmonate was added on day 4 to the suspension cultures of P. notoginseng cells. It was observed that, jasmonates greatly enhanced the ginsenoside content and the ratio of Rb group to Rg group (i.e. $(Rb_1\;+\;Rd)/(Rg_1\;+\;Re)$ in the P. notoginseng cells. Some of the synthetic jasmonates, such as pentafluoropropyl jasmonate (PFPJA), 2-hydroxyethyl jasmonate (HEJA) and 2-hydroxye-thoxyethyl jasmonate (HEEJA), could promote the ginsenoside content to $2.55\;\pm\;0.11,\;3.65\;\pm\;0.13\;and\;2.94\;\pm\;0.06$mg/100 mg DW, respectively, compared to that of $0.64\;\pm\;0.06$mg/100 mg DW for the control and $2.17\;\pm\;0.04$ mg/100 mg DW by the commercially available methyl jasmonate (MJA); and they could change the respective Rb:Rg ratio to $1.60\;\pm\;0.04,\;1.87\;\pm\;0.01\;and\;1.56\;\pm\;0.05$, compared to that of $0.47\;\pm\;0.01$ for the control and $1.42\;\pm\;0.06$ by MJA. The results suggest that suitable esterification of MJA with fluorine or hydroxyl group could in-crease the elicitation activity to induce plant secondary metabolism. The information obtained from this study is useful for hyper-production of heterogeneous products by plant cell cultures.

Effects of Methyl Jasmonate on Ethylene Producton in Tomato (Lycopersicon esculentum Mill.) Hypocotyl Segments and Fruits (Methyl jasmonate가 토마토(Lycopersicon esculentum Mill.)하배축 절편과 열매에서 에틸렌 생성에 미치는 영향)

  • June Seung Lee
    • Journal of Plant Biology
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    • v.38 no.3
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    • pp.235-242
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    • 1995
  • Effects of methyl jasmonate (MeJA) on ethylene production in tomato(Lycopersicon esculentum Mill.) hypocotyl segments and fruits were studied. Ethylene production in tomato hypocotyl segments was inhibited by the increasing concentratons of MeJA, and 450 $\mu$M of MeJA showed 50% inhibitory effect. Time course data indicate that this inhibitory effect of MeJA appeared after 3 h of incubation period and continued until 24 h. Inhibition of ethylene producton by MeJA was due to the decrease in 1-aminocyclopropane-1-carboxylic acid(ACC) synthase activity. However, MeJA treatment had no effect on ACC oxidase activity and the accumulaton of ACC oxidase mRNAs. MeJA also inhibited auxin-induced ethylene production by decreasing in ACC synthase activity. In contrast, MeJA stimulated ethylene production in tomato fruits. When 30 $\mu$L/mL MeJA was treated in a gaseous state, ethylene production doubled and this stimulating effect continued until 4 days. To investigate the mechanisms of MeJA on ethylene production, ACC synthase and ACC oxidase activities were examined after MeJA treatment. MeJA increased the activities of both ACC synthase and ACC oxidase, and induced ACC oxidase mRNA accumulation. These data suggest that MeJA plays distinct roles in the ethylene production in different tomato tissues. It is possible that MeJA affects differently the mechanisms of signal transuction leading to the ethylene biosynthesis.

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Effect of elicited by methyl jasmonate on the saponin contents of Codonopsis lanceolata (Elicitor처리가 더덕사포닌 함량에 미치는 영향)

  • Kim, Ji-Ah;Bae, Kee-Hwa;Choi, Yong-Eui
    • Journal of Plant Biotechnology
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    • v.42 no.3
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    • pp.265-270
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    • 2015
  • The roots of Codonopsis lanceolata (Campanulaceae) contain several kinds of triterpenoid saponin with high medicinal values, which have been used in traditional medicines. This study investigates the impacts of methyl jasmonate (MeJA) - adding time on the saponin synthesis and the hairy root growth of C. lanceolata. A significant decrease in major saponin (lancemaside of three kinds) content of hairy roots was observed with MeJA treatments. Contents of lancemaside A, B and E decreased about 15% more than non-treated hairy roots. In contrast, minor saponin (foetidissimoside A and aster saponin Hb) accumulation was about 15% higher than the non-treated hairy roots. These results suggest that MeJA treatment could be used in the production of teriterpene saponins.

Cyclic Dipeptides from Bacillus vallismortis BS07 Require Key Components of Plant Immunity to Induce Disease Resistance in Arabidopsis against Pseudomonas Infection

  • Noh, Seong Woo;Seo, Rira;Park, Jung-Kwon;Manir, Md. Maniruzzaman;Park, Kyungseok;Sang, Mee Kyung;Moon, Surk-Sik;Jung, Ho Won
    • The Plant Pathology Journal
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    • v.33 no.4
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    • pp.402-409
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    • 2017
  • Cyclic dipeptides (CDPs) are one of the simplest compounds produced by living organisms. Plant-growth promoting rhizobacteria (PGPRs) also produce CDPs that can induce disease resistance. Bacillus vallismortis strain BS07 producing various CDPs has been evaluated as a potential biocontrol agent against multiple plant pathogens in chili pepper. However, plant signal pathway triggered by CDPs has not been fully elucidated yet. Here we introduce four CDPs, cyclo(Gly-L-Pro) previously identified from Aspergillus sp., and cyclo(L-Ala-L-Ile), cyclo(L-Ala-L-Leu), and cyclo(L-Leu-L-Pro) identified from B. vallismortis BS07, which induce disease resistance in Arabidopsis against Pseudomonas syringae infection. The CDPs do not directly inhibit fungal and oomycete growth in vitro. These CDPs require PHYTOALEXIN DEFICIENT4, SALICYLIC ACID INDUCTION DEFICIENT2, and NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 important for salicylic acid-dependent defense to induce resistance. On the other hand, regulators involved in jasmonate-dependent event, such as ETHYLENE RECEPTOR1, JASMONATE RESPONSE1, and JASMONATE INSENSITIVE1, are necessary to the CDP-induced resistance. Furthermore, treatment of these CDPs primes Arabidopsis plants to rapidly express PATHOGENESIS-RELATED PROTEIN4 at early infection phase. Taken together, we propose that these CDPs from PGPR strains accelerate activation of jasmonate-related signaling pathway during infection.

Effects of compounds related to signal transduction on anthraquinone biosynthesis in transformed cells of Rubia cordifolia var. pratensis (형질전환시킨 갈퀴꼭두서니 세포의 색소생합성에 미치는 수종의 신호전달 cascade 관련물질의 효과)

  • Lyu, Lee;Shin, Seung-Won
    • Korean Journal of Pharmacognosy
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    • v.31 no.2
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    • pp.235-239
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    • 2000
  • The effects of several compounds related to signal transduction cascade were determined to induce the production of alizarin and purpurin in the hairy root culture system of Rubia cordifolia var. pratensis. It was found that out of five tested compounds jasmonic acid(1 mg/l) and methyl jasmonate(1 mg/l) stimulated strongly the biosynthesis of the pigments while linolenic acid(1 mg/l) induced no significant increase of the product. Yeast extract(600 mg/l) and arachidonic acid(1 mg/l) showed relatively mild inducing effects on production of alizarin. The effects of jasmonic acid and methyl jasmonate were reduced by treatment with cycloheximide(2.8 mg/l).

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