• Title, Summary, Keyword: Immobilization

Search Result 1,316, Processing Time 0.054 seconds

Enhanced Production of hGM-CSF by Immobilized Transgenic Plant Cell Cultures (형질전환된 식물세포에서 고정화 방법을 통한 hCM-CSF의 생산성 증대 연구)

  • Noha, Yun-Sook;Nama, Hyung-Jin;Choi, Hong-Yeol;Tak, Sa-Ra;Kim, Dong-Il
    • KSBB Journal
    • /
    • v.30 no.2
    • /
    • pp.82-90
    • /
    • 2015
  • Plant cell immobilization can protect plant cells from shear forces and increase the stability of gene. An additional advantage of immobilization is the easiness for performing continuous culture with cell recycling. Therefore plant cell immobilization can overcome the limitations of plant cell applications. In addition, target protein should be selected from pharmaceutical proteins to get rid of low expression level problem. The enhanced production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in immobilized Nicotiana tabacum suspension cell cultures. When the cells were immobilized in polyurethane foam, specific production of hGM-CSF was higher than that in alginate bead immobilization. Optimum continuous culture condition was the addition of 60 g/L sucrose in growth media with exchanging media every 6 day. Under the same condition, specific hGM-CSF production was 7 times higher in a 500-mL spinner flask than that in 100-mL Erlenmeyer flasks. Therefore, development of an effective immobilization process would be possible when the advantage of easy cell recycling was used. Consequently, enhanced production of target proteins could be possible in immobilized continuous cultures when the advantages of immobilization were applied.

Protective effects of Korean Red Ginseng against sub-acute immobilization stress-induced testicular damage in experimental rats

  • Lee, Sang-Ho;Choi, Kyung-Hwa;Cha, Kyu-Min;Hwang, Seock-Yeon;Park, Un-Kyu;Jeong, Min-Sik;Hong, Jae-Yup;Han, Chang-Kyun;In, Gyo;Kopalli, Spandana Rajendra;Kim, Si-Kwan
    • Journal of Ginseng Research
    • /
    • v.43 no.1
    • /
    • pp.125-134
    • /
    • 2019
  • Background: Excessive stress causes varied physiological and psychological disorders including male reproductive problems. Here, we attempted to investigate the protective effects of Korean Red Ginseng (Panax ginseng Meyer; KRG) against sub-acute immobilization stress-induced testicular damage in experimental rats. Methods: Male rats (age, 4 wk; weight, 60-70 g) were divided into four groups (n = 8 in each group): normal control group, immobilization control group, immobilization group treated with 100 mg/kg of KRG daily, and immobilization group treated with 200 mg/kg of KRG daily. Normal control and immobilization control groups received vehicle only. KRG (100 mg/kg and 200 mg/kg) was mixed in the standard diet powder and fed daily for 6 mo. Parameters such as organ weight, blood chemistry, sperm kinematic values, and expression levels of testicular-related molecules were measured using commercially available kits, Western blotting, and reverse transcription polymerase chain reaction. Results: Data revealed that KRG restored the altered testis and epididymis weight in immobilization stress-induced rats significantly (p < 0.05). Further, KRG ameliorated the altered blood chemistry and sperm kinematic values when compared with the immobilization control group and attenuated the altered expression levels of spermatogenesis-related proteins (nectin-2, cAMP responsive element binding protein 1, and inhibin-${\alpha}$), sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor), and antioxidant-related enzymes (glutathione S-transferase m5, peroxiredoxin-4, and glutathione peroxidase 4) significantly in the testes of immobilization stress-induced rats. Conclusion: KRG protected immobilization stress-induced testicular damage and fertility factors in rats, thereby indicating its potential in the treatment of stress-related male sterility.

Anti-stress and Anti-nociceptive Effects of the Aqueous Extracts of Chungsimyeonja-eum against Immobilization Stress and Incision Pain in Rats (청심포자음(淸心蒲子飮)의 구속 스트레스 및 절개 통증에 대한 항스트레스 작용과 진통효과)

  • Yoon, Jeong-Hwan;Ko, Il-Gyu;Shin, Mal-Soon;Kim, Chang-Ju;Lee, Chung-Yeol
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.21 no.4
    • /
    • pp.874-883
    • /
    • 2007
  • The aqueous extract of Chungsimyeonja-eum has been used to treat palpitation, anemia, chronic fatigue, hypertension, and stroke. It also possesses various pharmacological effects including hypotensive, blood circulating, sedative, anti-inflammatory, diuretic, and anti-stress activities. In the present results, the latency time of plantar test was decreased in the immobilization stress and incusion pain-induction group. However the paw withdrawal latency values were increased in the immobilization stress and incision pain-induction groups after treatment with the aqueous extract of Chungsimyeonja-eum. The c-Fos expression in the paraventricular nucleus (PVN), and 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase (TPH) in the dorsal raphe were enhanced in the immobilization stress and incision pain-induction groups. However, the treatment with the aqueous extract of Chungsimyeonja-eum suppressed the immobilization stress and incision pain-induced increase of c-Fos, 5-HT, and TPH expressions. Here in this study, we have demonstrated the protective effects of Chungsimyeonja-eum on immobilization and incision pain-induced stress. The present study revealed that Chungsimyeonja-eum treatment diminishes immobilization and pain stress.

Effect of Ginsenoside Re on Depression- and Anxiety-Like Behaviors and Cognition Memory Deficit Induced by Repeated Immobilization in Rats

  • Lee, Bom-Bi;Shim, In-Sop;Lee, Hye-Jung;Hahm, Dae-Hyun
    • Journal of Microbiology and Biotechnology
    • /
    • v.22 no.5
    • /
    • pp.708-720
    • /
    • 2012
  • In this study, we assessed the effects of ginsenoside Re (GRe) administration on repeated immobilization stress-induced behavioral alterations using the forced swimming test (FST), the elevated plus maze (EPM), and the active avoidance conditioning test (AAT). Additionally, we examined the effect of GRe on the central adrenergic system by observing changes in neuronal tyrosine hydroxylase (TH) immunoreactivity and brain-derived neurotrophic factor (BDNF) mRNA expression in the rat brain. Male rats received 10, 20, or 50 mg/kg GRe (i.p.) 30 min before daily exposures to repeated immobilization stress (2 h/day) for 10 days. Activation of the hypothalamic-pituitary-adrenal (HPA) axis in response to repeated immobilization was confirmed by measuring serum levels of corticosterone (CORT) and the expression of corticotrophin-releasing factor (CRF) in the hypothalamus. Repeated immobilization stress increased immobility in the FST and reduced open-arm exploration in the EPM test. It also increased the probability of escape failures in the AAT test, indicating a reduced avoidance response. Daily administration of GRe during the repeated immobilization stress period significantly inhibited the stress-induced behavioral deficits in these behavioral tests. Administration of GRe also significantly blocked the increase in TH expression in the locus coeruleus (LC) and the decrease in BDNF mRNA expression in the hippocampus. Taken together, these findings indicate that administration of GRe prior to immobilization stress significantly improved helpless behaviors and cognitive impairment, possibly through modulating the central noradrenergic system in rats. These findings suggest that GRe may be a useful agent for treating complex symptoms of depression, anxiety, and cognitive impairment.

Purification and Immobilization of Cyclodextrin glucanotransferase from recombinant Bacillus subtilis

  • Seo, Hyo-Jin;Kim, Yeong-Hwa;Kim, Seong-Gu
    • 한국생물공학회:학술대회논문집
    • /
    • /
    • pp.671-674
    • /
    • 2001
  • Cyclodextrin glucanotransferase(CGTase) derived from recombinant Bacillus subtilis was partial purified and concentrated by ultrafiltration. The prepared CGTase were immobilized on various matrices by ionic interaction or covalent bond. CGTase covalently bound on CNBr-activated sepharose 4B were identified to be the highest immobilization activity among various immobilization methods. The optimum conditions for CGTase immobilization were determined; $30^{\circ}C$, 6Orpm, using O.2g CNBr-activated sepharose 4B in pH 6.0 phosphate buffer and 9hr immobilization.

  • PDF

Immobilization of MTBE using cyclodextrins

  • Baek, Ki-Tae;Yang, Ji-Won
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
    • /
    • /
    • pp.120-123
    • /
    • 2003
  • Immobilization behavior of methyl tert-butyl ether (MTBE) by various cyclodextrins(CDs) was studied to investigate the feasibility of MTBE removal using cyclodexrins. Even though MTBE has relatively low hydrophobicity and higher polarity compared to other organics, it was effectively immobilized by CDs. The immobilization isotherms was shown as a type of Freundlich isotherms, and the immobilization capacity of -CDs was the largest among natural COs. The initial apparent association constant for MTBE-CD complex follows the order : gamma = beta > methyl-beta > hydroxypropyl beta > alpha. These differences of the constants are related to the size of MTBE and CDs. The size of beta-CD and gamma-CD is large to encapsulate MTBE molecule into the cavity, which that of alpha-CB is too small to encapsulate MTBE.

  • PDF

Investigation on the Rheological Properties of Coating Colors by immobilization cell (Immobilization cell을 이용한 도공액 특성 연구)

  • Park, Si-Han;Kim, Gyeong-Dong;Kim, Song-Ju;Heo, Yong-Seong
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
    • /
    • /
    • pp.139-147
    • /
    • 2003
  • Coater runnability of the paper coating and final product properties can be affected by the immobilization of coating color and the dewatering into the base paper. During the dewatering, the rheological properties and solids content of a coating are dramatically changed. For the purpose of obtaining better coater runnability and high quality of coater paper many papermakers are trying to improve the water retention of paper coatings by using some additives such as thickener and co-binder. In this study, we tried to investigate the rheological properties and the immobilization point of coatings with immobilization cell during the dewatering of coatings.

  • PDF

Microarray Analysis of Gene Expression by Ginseng Water Extracts in a Mouse Adrenal Cortex after Immobilization Stress

  • Kim, Young-Ock;Lee, Sang-Won
    • Journal of Ginseng Research
    • /
    • v.35 no.1
    • /
    • pp.111-123
    • /
    • 2011
  • To investigate the effects of repeated immobilization-stress challenge on the the hypothalamic-pituitary-adrenal axis, the genomic transcriptome in the adrenal cortex of immobilization-stressed mouse was analyzed by using a cDNA microarray. Mice were subjected to immobilization stress for 2 h per day for 5 consecutive d. With a 4.0-fold cutoff of arbitrary criteria, the expression levels of 168 out of 41,174 genes were significantly modulated in the adrenal cortex by stress when comparing the control and experimental groups. These genes were related to apoptosis, cell cycle, immune response, inflammatory responses, and signal transduction, and thus may be used as potential targets for the development of therapeutics for chronic stress or depression. Six significant genes among these were selected for real time polymerase chain reaction analysis to confirm the change of their expression levels. The gene for phospho 1 was also further investigated because its expression showed the greatest fold-change.

Effect of Adjuvant Administration on Gastrocnemius Atrophy Induced by Ankle Joint Fixation in Rats (흰쥐 발목고정으로 유발된 비복근 위축에서 보조약물 투여가 미치는 효과)

  • Kim, Bumhoi;Lee, Taesik
    • Journal of The Korean Society of Integrative Medicine
    • /
    • v.5 no.4
    • /
    • pp.49-55
    • /
    • 2017
  • Purpose : In this study we tested the hypothesis that eucommia ulmoides (EU) extract would improve disuse-induced muscle atrophy following hindlimb immobilization. Method : The hindlimb immobilization was performed with casting tape to keep the left ankle joint in a fully extended position. The rats in EU treated group were orally administrated with eucommia ulmoides water extract. After 2 weeks of immobilization, all animals were sacrificed, and the whole gastrocnemius muscles were dissected from both legs. The morphology of right and left gastrocnemius muscles in both EU and control groups were assessed by hematoxylin and eosin staining. Results : Eucommia ulmoides extract represented the significant protective effects against the reductions of the gastrocnemius muscles weight and average cross section area to compared with Control group. Conclusion : Eucommia ulmoides has protective effects against immobilization induced muscle atrophy.

Immobilization of Penicillium citrinum by Entrapping Cells in Calcium Alginate for the Production of Neo-Fructooligosaccharides

  • Lim, Jung-Soo;Park, Seung-Won;Lee, Jin-Won;Oh, Kyeong-Keon;Kim, Seung-Wook
    • Journal of Microbiology and Biotechnology
    • /
    • v.15 no.6
    • /
    • pp.1317-1322
    • /
    • 2005
  • This work describes neo-fructooligosaccharides (neo-FOSs) production using the immobilized mycelia of Penicillium citrinum. Some critical factors were evaluated to optimize maximal production of neo-FOS. Optimal alginate and cell concentrations were determined to be $1.96\%$ alginate and $7.17\%$ cell, respectively, by statistical analysis. The optimal concentration of $CaCl_{2}$, which is related to bead stability, was determined to be 2 M. It was possible to increase the neo-FOS production by adding 15 units of glucose oxidase to the batch reaction. By co-immobilizing cells and glucose oxidase, neoFOS productivity increased $123\%$ compared with the whole-cell immobilization process. Based on the results above, a co-immobilization technique was developed and it can be utilized for large-scale production.