• Title, Summary, Keyword: Immobilization

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Effects of Glycyrrhizae Radix on Serum Corticosterone and Blood Histamine Content by Immobilization Stress in Mice (감초(甘草) 엑기스가 Immobilization Stress 부하(負荷)후 혈중(血中) Corticosterone 및 Histamine 함량변화(含量變化)에 미치는 영향)

  • Eun, Jae-Soon;Oh, Chan-Ho;Han, Jong-Hyun
    • Korean Journal of Pharmacognosy
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    • v.20 no.1
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    • pp.37-42
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    • 1989
  • This study was carried out to investigate the effect of Glycyrrhizae Radix on serum corticosterone and blood histamine content by immobilization stress in mice. Corticosterone secretion and blood histamine level was significantly increased in mice by subjecting the animals to immobilization stress 1 hr. after intraperitoneal injection of Glycyrrhizae Radix extract (150 mg/kg) and glycyrrhizinic and (15 mg/kg). whereas, administration of cortisol $(7.5\;{\mu}g/kg)$ provoked a decrease in corticosterone secretion and histamine levels. These results suggested that glycyrrhizinic acid was effective on cortiosterone release provoked by immobilization stress and this release was mediated in part by histamine.

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Effect of Anjungtang on the Contents of Catecholamine in Plasma and Urine of Rats Stressed by Immobilization (안정탕(安定湯)이 구속(拘束)Stress 흰쥐의 혈액(血液) 및 뇨(尿) Catecholamine 함량(含量)에 미치는 영향(影響))

  • Lee Jeong-Ho;Chung Dae-Kyoo
    • Journal of Oriental Neuropsychiatry
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    • v.6 no.1
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    • pp.51-59
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    • 1995
  • This experiments were conducted to study anti-stress effects of Anjungtang on the contents of catecholamine in plasma and urine of rats stressed by immobilization. The main results, obtained were summerized as follow: 1 The contents of plasma and urine catecholamine increased significantly in the group of rats stressed by immobilization. 2 The contents of plasma catecholamine decreased with statistical significance in the group administered Anjungtang of rats stressed by immobilization compared with in the group administered non Anjungtang. 3.The contents of urine catecholamine decreased with in the group administered Anjungtang of rats stressed by immobilization compared with in the group administered non Anjungtang. Particulaly the content of urine norepinephrine decreased with statistical significance. 4. The weight of the body increased in the group administered Anjungtang of rats stressed immobilization compared with in the group administered non Anjungtang. According to the above results, this experiments concluded that Anjungtang had significant effecrs in reducing stress.

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Optimal Immobilization of Penicillinase for Ion-selective Electrode

  • Hur, Moon-Hye;Kang, Hee-Jin;Min, Hye-Young;Lee, Ji-Yeun;Lee, Ki-Hyun;Ahn, Moon-Kyu
    • Archives of Pharmacal Research
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    • v.20 no.1
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    • pp.68-71
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    • 1997
  • Penicillin sensor was prepared by immobilizing penicillinase (Pcase) on $H^{+}$-selective carboxylated poly (vinyl chloride) (PVC-COOH) membrane or cellulose filter membrane. The immobilization techniques are as follows. Pcase was immobilized with GTH on $H^{+}$-selective PVC-COOH membrane or some amount of BSA was dropped on that membrane. Another method to make immobilization is to mix type I Pcase with GTH and drop on a cellulose filter membrane. According to immobilization techniques, there were some differences in response properties of enzyme electrodes, however, all electrodes responded to Pcase-resistant penicillin derivatives. Pcase immobilized on cellulose filter membrane with $H^{+}$-selective PVC membrane eletrode was more stable and more sensitive to penicillinase-resistant penicillin derivatives than any other immobilization techniques.

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Review for Immobilization Methods of Biosorbent (생물흡착제의 고정화 방법에 대한 고찰)

  • Jeon, Choong
    • Journal of the Korea Organic Resources Recycling Association
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    • v.19 no.2
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    • pp.16-21
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    • 2011
  • Immobilization of biosorbent is very important for application to real wastewater treatment process because biosorbent itself does not have enough tough structure. Therefore, resent research on heavy metal biosorption using biomass has been focused on its efficient immobilization method. To improve the mechanical strength of freely biosorbent, many immobilization methods have been suggested for applications to the biosorbent such as microorganisms or polysaccharides. In this study, various immobilization methods such as adsorption, covalent binding, entrapment, encapsulation, and crosslinking will be introduced.

Effects of Hwaganjeon on Immobilization-Stress or Cold-Stress in Mice (화간전(化肝煎)이 생쥐의 Immobilization-Stress 및 Cold-Stress에 미치는 영향(影響))

  • Park, Jong-Moon;Ko, Jeong-Min;Ahn, Kyu-Hwan;Choe, Chang-Min;Yoo, Sim-Keun
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.4
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    • pp.93-116
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    • 2006
  • Purpose : This study is to examine the effects of Hwaganjeon water extract(HGJ) on immobilization-stress or cold-stress in BALB/C mice. Methods : We have Hwaganjeon water extract(HGJ) by freeze-dryer & melt it by a saline solution. We feed HGJ 500mg/Kg to 5mice, and add immobilization-stress by putting mice in plastic cylinder 10 hours, and add cold-stress by putting mice in $4^{\circ}C$ cold room 6 hours. Results : 1. HGJ decreased the serum level of histamine and corticosterone increased by immobilization-stress or cold-stress. HGJ inhibited the release of histamine from mast cells at the concentration of 1 mg/ml. 2. HGJ did not affect the cell viability of thymocytes decreased by immobilization-stress or cold-stress, but increased the cell viability of splenocytes decreased by immobilization-stress or cold-stress. 3. HGJ decreased the population of splenic $CD4^{+}$ and $CD8^{+}$cells increased by immobolization-stress or cold-stress. 4. HGJ enhanced the production of ${\gamma}$-interferon(IFN) and interleukin(IL)-2 decreased by immobilization-stress or cold-stress. Conclusion : These results indicate that HGJ may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.

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Effect of Sulfonylureas Administered Centrally on the Blood Glucose Level in Immobilization Stress Model

  • Sharma, Naveen;Sim, Yun-Beom;Park, Soo-Hyun;Lim, Su-Min;Kim, Sung-Su;Jung, Jun-Sub;Hong, Jae-Seung;Suh, Hong-Won
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.3
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    • pp.197-202
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    • 2015
  • Sulfonylureas are widely used as an antidiabetic drug. In the present study, the effects of sulfonylurea administered supraspinally on immobilization stress-induced blood glucose level were studied in ICR mice. Mice were once enforced into immobilization stress for 30 min and returned to the cage. The blood glucose level was measured 30, 60, and 120 min after immobilization stress initiation. We found that intracerebroventricular (i.c.v.) injection with $30{\mu}g$ of glyburide, glipizide, glimepiride or tolazamide attenuated the increased blood glucose level induced by immobilization stress. Immobilization stress causes an elevation of the blood corticosterone and insulin levels. Sulfonylureas pretreated i.c.v. caused a further elevation of the blood corticosterone level when mice were forced into the stress. In addition, sulfonylureas pretreated i.c.v. alone caused an elevation of the plasma insulin level. Furthermore, immobilization stress-induced insulin level was reduced by i.c.v. pretreated sulfonylureas. Our results suggest that lowering effect of sulfonylureas administered supraspinally against immobilization stress-induced increase of the blood glucose level appears to be primarily mediated via elevation of the plasma insulin level.

Effects of Kwibitang on the Specific Immune Response after Immobilization Stress in C57BL/6 Mice (귀비탕이 C57BL/6 Mouse에 Stress 부하 후 특이적 면역반응에 미치는 영향)

  • Lee Taek Yul;Han Mi Sook;Oh Chan Ho;Eun Jae Soon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.5
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    • pp.1208-1216
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    • 2003
  • Stress is known to influence the immune function via an effect on the central nervous system. To investigated the effects of Kwibitang water extract (KBT) on the specific immune response in C57BL/6 mice stressed by immobilization, we evaluated the changes in the cell viability, DNA fragmentation and subpopulation of thymocytes and splenocytes. KBT enhanced the cell viability of thymocytes and splenocytes decreased by immobilization stress. Also, KBT decreased DNA fragmentation of thymocytes and splenocytes increased by immobilization stress. KBT decreased the population of CD4/sup +/ cells and CD8/sup +/ cells in thymocytes and Thy1/sup +/ cells in splenocytes increased by immobolization stress, but increased the population of B220/sup +/ cells decreased by immobilization stress. In addition, KBT enhanced the production of ν-interferon and IL-2 decreased by immobilization stress. These results indicate that KBT may be useful for the prevention and treatment of stress via enhancement of the specific immune response

Functionalized Poplar Powder as a Support Material for Immobilization of Enoate Reductase and a Cofactor Regeneration System

  • Li, Han;Cui, Xiumei;Zheng, Liangyu
    • Journal of Microbiology and Biotechnology
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    • v.29 no.4
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    • pp.607-616
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    • 2019
  • In this study, functionalized poplar powder (FPP) was used as a support material for the immobilization of enoate reductase (ER) and glucose-6-phosphate dehydrogenase (GDH) by covalent binding. Under optimal conditions, the immobilization efficiency of ER-FPP and GDH-FPP was 95.1% and 84.7%, and the activity recovery of ER and GDH was 47.5% and 37.8%, respectively. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis indicated that FPP was a suitable carrier for enzyme immobilization. ER-FPP and GDH-FPP exhibit excellent thermal stabilities and superior reusability. Especially, ER-FPP and GDH-FPP enable the continuous conversion of 4-(4-Methoxyphenyl)-3-buten-2-one with $NAD^+$ recycling. While the immobilization strategies established here were simple and inexpensive, they exploited a new method for the immobilization and application of ER and its cofactor recycling system.

Optimization of the Condition of Immobilized Photobacterium phosphoreum with Strontium Alginate (Strontium Alginate를 담체로 한 Photobacterium phosphoreum 고정화 조건의 최적화)

  • 이홍주;김현숙;정계훈;이은수;전억한
    • Microbiology and Biotechnology Letters
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    • v.27 no.2
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    • pp.136-144
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    • 1999
  • Since the condition of immobilization must be optimized, it is very important to know whether and on how conditions bacterial cells retain their metabolic activity during immobilization process. A bioluminescence intensity had the maximum value when cell concentrations were between 1.0 and 1.2 measured at O.D660. The strontium alginate was used as an immobilization matrix and two independent factors for immobilization of Photobacterium phosphoreum with strontium alginate were optimized with the response surface methodology(RSM) considering degree of bioluminescence. As a result, the optimum concentration for immobilization was found to be 2.4%(w/w) for sodium alginate and 0.31M for strontium chloride, respectively. A dilution was carried out with 2.5%(w/v) NaCl solution that is an optimum environmental condition for growth of P. phosphoreum. Under the such condition of immobilization, hardness could be predicted as 4.66$\times$104N/$m^2$ and it took different time according to the volume of matrix to be immobilized completely.

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Alpha-Amylase Immobilization on Epoxy Containing Thiol-Ene Photocurable Materials

  • Cakmakci, Emrah;Danis, Ozkan;Demir, Serap;Mulazim, Yusuf;Kahraman, Memet Vezir
    • Journal of Microbiology and Biotechnology
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    • v.23 no.2
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    • pp.205-210
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    • 2013
  • Thiol-ene polymerization is a versatile tool for several applications. Here we report the preparation of epoxide groups containing thiol-ene photocurable polymeric support and the covalent immobilization of ${\alpha}$-amylase onto these polymeric materials. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) coupled with SEM was used to explore the chemical composition. The polymeric support and the immobilization of the enzyme were characterized by FTIR analysis. SEM-EDS and FTIR results showed that the enzyme was successfully covalently attached to the polymeric support. The immobilization efficiency and enzyme activity of ${\alpha}$-amylase were examined at various pH (5.0-8.0) and temperature ($30-80^{\circ}C$) values. The storage stability and reusability of immobilized ${\alpha}$-amylase were investigated. The immobilization yield was $276{\pm}1.6$ mg per gram of polymeric support. Enzyme assays demonstrated that the immobilized enzyme exhibited better thermostability than the free one. The storage stability and reusability were improved by the immobilization on this enzyme support. Free enzyme lost its activity completely within 15 days. On the other hand, the immobilized enzyme retained 86.7% of its activity after 30 days. These results confirm that ${\alpha}$-amylase was successfully immobilized and gained a more stable character compared with the free one.