• Title, Summary, Keyword: Hyphantria cunea

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Studies on the Toxicity of $\delta$-endotoxin of Bacillus thuringiensis to the Several Tissues of Hyphantria cunea Drury (미국흰불나방(Hyphantria cunea Drury)에 대한 Bacillus thuringiensis 내독소단백질의 독성효과에 관한 연구)

  • 전향미;조자향;강석권;서숙재
    • Journal of Sericultural and Entomological Science
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    • v.37 no.1
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    • pp.62-67
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    • 1995
  • Ultrastructural changes of tissues caused by Bacillus thuringiensis var. kurstaki $\delta$-endotoxin intoxication of Hyphantria cunea were observed by transmission electron microscopy. Bt $\delta$-endotoxin crystals induced the disruption of microvilli, vacuolation of cytoplasm, changes in the cisternae of the endoplasmic reticulum, disappearance of basal striations, loss of ribosomes, and changes in the configurations of mitochondria in the columnar cell of midgut. The fat body cells also showed spherical endoplasmic reticulum and distorted mitochondria, and then the cells were destroyed.

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The Effect of $\delta$-endotoxin of Bacillus thuringiensis on the Protein Synthesis of the Several Tissues of Hyphantria cunea Drury (Bacillus thuringiensis 내독소단백질이 미국흰불나방 조직의 단백질 합성에 미치는 영향)

  • 전향미;강석권;서숙재
    • Journal of Sericultural and Entomological Science
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    • v.37 no.1
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    • pp.68-73
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    • 1995
  • The toxic effect of Bacillus thuringiensis var, kurstaki $\delta$-endotoxin was determined in the midgut, fat body and Malpighian lobules from larvae of Hyphantria cunea using the au-toradiography and Western blot methods. Bt $\delta$-endotoxin crystals inhibited protein synthesis and elongation factor-2 activity of all tissues tested.

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The Production and Characterization of Monoclonal Antibodies to the Major Polyhedra Inclusion Body of the Occluded Form of Hyphantria cunea Nuclear Polyhedrosis Virus (Hyphantria cunea Nuclear Polyhedrosis Virus 봉입형의 핵다면체에 대한 단일클론 항체의 생산과 인식항원의 결정)

  • 장성호;홍순복;이형환;김종배;조명환
    • Microbiology and Biotechnology Letters
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    • v.21 no.5
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    • pp.406-413
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    • 1993
  • This study was performed to produce monoclonal antibodies to the major polyhedral inclusion body (PIB) antigen of the occluded form of Hyphantria cunea nuclear polyhedrosis virus (HcNPV). PIB proteinS were purfied from the Spodoptera frugiperda cell infected with HcNPVs. Using the purified PIB protein, BALB/C mice were immunized 3 times with 2 weeks intervals. The spleen were removed and fused with Sp2/0-Ag14, mouse myeloma cells.

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Nucleotide Sequence Analyses of p10 Gene and its Promoter of Hyphantria cunea Nuclear Polyhedrosis Virus (Hyphantria cunea Nuclear Polyhedrosis Virus p10유전자와 프로모터의 염기서열 결정)

  • Park, Sun-A;Cha, Sung-Chul;Chang, Jae-Hyeok;Lee, Hyung-Hoan
    • The Journal of Korean Society of Virology
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    • v.26 no.1
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    • pp.131-137
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    • 1996
  • The sequences of p10 gene its promoter of Hyphantria cunea NPV were determined. According to the sequence analysis, the putative p10 gene ORF has 285 bp. The 5'-non-coding leader sequence of the p10 gene promoter contained the TATA box and the putative transcription initiation site TAAG motif. Poly (A) tail signals, AATAAA sequence was at site 65 base upstream from the 3' terminus. The deduced amino acid sequence of p10 protein was 95 with a predicted molecular weight of 10.26 kDa. In the p10 protein sequence, a hydrophobic region was present at the N-terminus of the protein, whereas the C-terminus was highly hydrophilic. The p10 protein of H. cunea NPV did not contain cysteine, histidine, trytophan, tryptophane, tyrosine, glutamine and asparagine residues.

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Molecular Characterization of Apolipophorin-III in the Fall Webworm, Hyphantria cunea Drury

  • Kim, Hong-Ja;Lee, Sang-Dae;Seo, Sook-Jae
    • International Journal of Industrial Entomology
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    • v.3 no.2
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    • pp.163-168
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    • 2001
  • We isolated and sequenced a cDNA clone corresponding to apolipophorin-III (apoLp-III) from the fall webworm, Hyphantria cunea. The cDNA for apoLp-III codes fer a 187-residue protein (561 bp) with a predicted molecular mass of 20 kDa. The calculated isoelectric point is 8.76. Multiple alignment analysis of the amino acid sequence revealed that H. cunea apoLp-III is most similar to that of Spodoptera litura (71.5% identity), followed by that of Manduca sexta (69.7% identity). They share five amphipathic $\alpha$-helices that are proposed to play a critical role in the binding of apoLp-III to lipophorin.

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Purification and Characterization of Apolipophorin-III from Haemolvmph of Fall Webworm Hvphantria cunea Drury (미국흰불나방(Hyphantria cunea Drury) 혈림프부터 apolipophorin-III의 순수정제 및 특성)

  • 윤화경;서신자김학열
    • The Korean Journal of Zoology
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    • v.37 no.4
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    • pp.488-494
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    • 1994
  • Apolipophorin-III (ApoLp-III) was purified from adult haemolynph of Hyphantriu cuneo and their molecular weight and synthetic place were investigated. ApoLp-III purification was performed by KBr-density gradient ultracentrifugation followed by gel permeation chromatographv (Sephadex G-1001 and ion-exchange chromatography (CM-52) and their purity was confirmed on 10% SDS-PAGE. ApoLp-III has the molecular weight of 18 ItDa and is synthesized by fat body.

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Characteristics and Pathogenicity of Hyphantria cunea Nuclear Polyhedrosis Virus (Hyphantria cunea Nuclear Polyhedrosis Virus의 특성 및 병원성)

  • Lee, Keun-Kwang;Kim, Myung-Kon;Park, Il-Woong
    • Applied Biological Chemistry
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    • v.38 no.5
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    • pp.435-439
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    • 1995
  • Some characteristics and pathogenicity of Hyphantria cunea nuclear polyhedrosis virus (HcNPV), a potential microbial pesticide was studied. H. cunea NPV replicated in the nucleus of S. frugiperda cells cultured in the TNMFH medium. In case of virus infected cell, prepolyhedra formation was observed at 24hrs post-infection. At 48 hrs post-infection, Most of the infected cell contained many mature polyhedra which were released into culture media 72 hrs post-infection, with the cells grown in suspension culture, pH of the culture medium increased during the virus replication: the pH of fresh medium was 6.35 and rose to 6.77 within 120 hrs. Polyhedra formed a band in linear density gradient of sucrose by centrifugation, which co-sedimented with $50{\sim}55%$ sucrose. The shape of the purified polyhedra was mostly tetragonal hexahedron and its size was about $2.5{\mu}m$. Electron microscopy and phase contrast microscopy showed that many bundled nucleocapsids were occluded in mature polyhedra at 48 hrs post infection. H. cunea larvae infected with NPV showed a higher motality in the second and third instar than in the fourth instar. Death rate of H. cunea larvae in the second and third instar fed with leaves coated with $1.5{\times}10^{9}{\sim}l.5{\times}10^{7}PIBs/ml$ reached more than 90%.

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Hyphantria cunea Nucleopolyhedrovirus, a Novel Baculovirus Isolated from Fall Webworm in Korea

  • Heo, Won-Il;Choi, Jae-Bang;Bae, Sung-Min;Shin, Tae-Young;Woo, Soo-Dong
    • International Journal of Industrial Entomology
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    • v.22 no.2
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    • pp.75-82
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    • 2011
  • To elucidate the novelty of Hyphantria cunea nucleopolyhedrovirus (HcNPV) isolated in Korea, polyhedrin and inhibitor of apoptosis (iap) gene structures were determined and analyzed. The analysis of HcNPV polyhedrin showed a little difference with 97.6% at the nucleotide level but no difference at the amino acid level when compared with that of previously reported H. cunea NPV (HycuNPV). On the other hand, iap genes showed variable differences with 89.0-99.6% nucleotide and 90.0-99.6% amino acid sequence identities. Especially, the 5' and 3' non-coding flanking sequences of iap1 gene had lower degree of identity with those of HycuNPV. Although the phylogenetic analyses using polyhedrin and iap genes showed that HcNPV is closely related with HycuNPV, we could provide that HcNPV is a novel isolate having novel gene structures.

Synthesis and Fate of Yolk Protein-3 in Hyphantria cunea D. (미국흰불나방(Hyphantria cunea D.)의 난황단백질-3의 합성 및 이용)

  • 이상대;김학열
    • The Korean Journal of Zoology
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    • v.34 no.3
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    • pp.394-402
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    • 1991
  • Yolk protein-3 (YP3) was purified from the ovary of Hvpharatria cunea D. and the synthesis and fate during embryogenesis of WP3 were investisated by electrophoresis and fluorography. YP3 purified through gel slice and electrophoretic elution'was determined to have M. W. of 18 Kd and consist of one subunit. Haemolymph and fat body of male and female %were electrophoresed during vifellogenic stages to indentifv the vitellosenin in female. The result showed that there was no distinct difference in electrophoretic patterns betweerl male and female. However, tissue culture of fat body and maturing ovary indicated that YP3 was svuthesized by fat body. Aiso, vP3 in iaid eggs was maintained constant untii naut s artier oviposition and then decreased, indicating that YP3 was drastically used during late embryosenesis. However, a part of YP3 was present even in newly hatched first instar larvae.

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Hyphantria Cunea Nuclear Polyhedrosis Virus의 ts 돌연변이체의 분리

  • 이근광;조일환;이형환
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • pp.513.1-513
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    • 1986
  • Hyphantria Cunea NPV는 (흰불나방 바이러스) 유전적 기능을 알기 위하여 돌연변이 유발물질인 Nitrosoguanidine를 처리하여 돌연변이를 유발시킨 후 허용온도인 $25^{\circ}C$와 제한온도인 32$^{\circ}C$에서 Plaque assay하여 온도민감성 돌연변이체 13균주를 (ts-N220, -N 258, -N606, -N649 -N877 -N1420, -N1488, -N1491, -N1498, -N1512, N-2065,-N2076, -N2085) 를 분리 하였다.

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