• Title, Summary, Keyword: Hunan

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Ferric Reductase Activity of the ArsH Protein from Acidithiobacillus ferrooxidans

  • Mo, Hongyu;Chen, Qian;Du, Juan;Tang, Lin;Qin, Fang;Miao, Bo;Wu, Xueling;Zeng, Jia
    • Journal of Microbiology and Biotechnology
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    • v.21 no.5
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    • pp.464-469
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    • 2011
  • The arsH gene is one of the arsenic resistance system in bacteria and eukaryotes. The ArsH protein was annotated as a NADPH-dependent flavin mononucleotide (FMN) reductase with unknown biological function. Here we report for the first time that the ArsH protein showed high ferric reductase activity. Glu104 was an essential residue for maintaining the stability of the FMN cofactor. The ArsH protein may perform an important role for cytosolic ferric iron assimilation in vivo.

Synthesis and Evaluation O-Benzyl Oxime-ether Derivatives Containing β-Methoxyacrylate Moiety for Insecticidal and Fungicidal Activities

  • Hu, Zhi-Bin;Luo, He-An;Wang, Xiao-Guang;Huang, Ming-Zhi;Huang, Lu;Pang, Huai-Lin;Mao, Chun-Hui;Pei, Hui;Huang, Chao-Qun;Sun, Jiong;Liu, Ping-Le;Liu, Ai-Ping
    • Bulletin of the Korean Chemical Society
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    • v.35 no.4
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    • pp.1073-1076
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    • 2014
  • In attempt to lead compounds exhibiting both insecticidal and fungicidal activities, a series of O-benzyl oximeether derivatives were designed and synthesized by introducing ${\beta}$-methoxyacrylate pharmacophore into a scaffold. The insecticidal activity against Aphis fabae and the fungicidal activity against Erysiphe graminis were screened. The title compounds exhibited remarkable insecticidal and fungicidal activities. The most potent compound 6d was identified. Its insecticidal $LC_{50}$ against A. fabae is 6.4 mg/L, which is lower than that of chlorfenapyr (19.4 mg/L) and even close to the level of imidacloprid (4.8 mg/L). Its fungicidal $EC_{90}$ in preventive and curative treatment against E. graminis are 2.2 and 4.8 mg/L, respectively, which are lower than azoxystrobin (7.0 and 5.9 mg/L). These results indicate that compound 6d can be considered as a lead for further developing new O-benzyl oxime-ether typed candidates with both fungicidal and insecticidal activities.

A Fault Tolerant Structure and Control Strategy for Electromagnetic Stirring Supplies

  • Li, Yan;Luo, An;Xiang, Xinxing;Chen, Yandong;He, Zhixing;Zhou, Fayun;Chen, Zhiyong
    • Journal of Power Electronics
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    • v.17 no.5
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    • pp.1256-1267
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    • 2017
  • A fault tolerant structure and its corresponding control strategy for electromagnetic stirring power supplies are proposed in this paper. The topology structure of the electromagnetic stirring power supply contains two-stages. The fore-stage is the PWM rectifier. The back-stage is the fault tolerant inverter, which is a two-phase three-bridge orthogonal inverter circuit while operating normally. When the power switch devices in the inverter are faulty, the structure of the inverter is reconfigured. The two-phase half bridge inverter circuit is constructed with the remaining power switch devices and DC-link capacitors to keep the system operating after cutting the faulty power switch devices from the system. The corresponding control strategy is proposed to let the system work under both normal and fault conditions. The reliability of the system is improved and the requirement of the electromagnetic stirring process is met. Finally, simulation and experimental results verify the feasibility of the proposed fault tolerant structure and corresponding control strategy.

A novel human KRAB-related zinc finger gene ZNF425 inhibits mitogen-activated protein kinase signaling pathway

  • Wang, Yuequn;Ye, Xiangli;Zhou, Junmei;Wan, Yongqi;Xie, Huaping;Deng, Yun;Yan, Yan;Li, Yongqing;Fan, Xiongwei;Yuan, Wuzhou;Mo, Xiaoyang;Wu, Xiushan
    • BMB Reports
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    • v.44 no.1
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    • pp.58-63
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    • 2011
  • Zinc finger (ZNF) proteins play a critical role in cell growth, proliferation, apoptosis, and intracellular signal transduction. In this paper, we cloned and characterized a novel human KRAB-related zinc finger gene, ZNF425, which encodes a protein of 752 amino acids. ZNF425 is strongly expressed in the three month old human embryos and then is almost undetectable in six month old embryos and in adult tissues. An EGFP-ZNF425 fusion protein can be found in both the nucleus and the cytoplasm. ZNF425 appears to act as a transcription repressor. Over-expression of ZNF425 inhibits the transcriptional activities of SRE, AP-1, and SRF. Deletion analysis indicates that the C2H2 domain is the main region responsible for the repression. Our results suggest that the ZNF425 gene is a new transcriptional inhibitor that functions in the MAPK signaling pathway.

Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

  • Deng, Yun;Li, Yongqing;Fan, Xiongwei;Yuan, Wuzhou;Xie, Huaping;Mo, Xiaoyang;Yan, Yan;Zhou, Junmei;Wang, Yuequn;Ye, Xianli;Wan, Yongqi;Wu, Xiushan
    • BMB Reports
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    • v.43 no.6
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    • pp.432-437
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    • 2010
  • LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes ${\alpha}B$-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with ${\alpha}B$-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, ${\alpha}B$-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or ${\alpha}B$-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both ${\alpha}B$-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and ${\alpha}B$-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21.

EXISTENCE AND EXPONENTIAL STABILITY OF ALMOST PERIODIC SOLUTION FOR SHUNTING INHIBITORY CELLULAR NEURAL NETWORKS WITH DISTRIBUTED DELAYS AND LARGE IMPULSES

  • Zuo, Yi;Wang, Yaonan;Huang, Lihong;Li, Chunsheng
    • Journal of the Korean Mathematical Society
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    • v.46 no.5
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    • pp.1071-1085
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    • 2009
  • This paper considers the problem of existence and exponential stability of almost periodic solution for shunting inhibitory cellular neural networks with distributed delays and large impulses. Based on the contraction principle and Gronwall-Bellman's inequality, some sufficient conditions are obtained. The results of this paper are new and they complement previously known results.

Cloning and characterization of the cardiac-specific Lrrc10 promoter

  • Fan, Xiongwei;Yang, Qing;Wang, Youliang;Zhang, Yan;Wang, Jian;Yuan, Jiajia;Li, Yongqing;Wang, Yuequn;Deng, Yun;Yuan, Wuzhou;Mo, Xiaoyang;Wan, Yongqi;Ocorr, Karen;Yang, Xiao;Wu, Xiushan
    • BMB Reports
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    • v.44 no.2
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    • pp.123-128
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    • 2011
  • Leucine-rich repeat containing protein 10 (LRRC10) is characterized as a cardiac-specific gene, suggesting a role in heart development and disease. A severe cardiac morphogenic defect in zebrafish morphants was recently reported but a contradictory result was found in mice, suggesting a more complicated molecular mechanism exists during mouse embryonic development. To elucidate how LRRC10 is regulated, we analyzed the 5'enhancer region approximately 3 kilo bases (kb) upstream of the Lrrc10 start site using luciferase reporter gene assays. Our characterization of the Lrrc10 promoter indicates it possesses complicated cis-and trans-acting elements. We show that GATA4 and MEF2C could both increase transcriptional activity of Lrrc10 promoter individually but that they do not act synergistically, suggesting that there exists a more complex regulation pattern. Surprisingly, knockout of Gata4 and Mef2c binding sites in the 5’enhancer region (-2,894/-2,889) didn't change the transcriptional activity of the Lrrc10 promoter and the likely GATA4 binding site identified was located in a region only 100 base pair (bp) upstream of the promoter. Our data provides insight into the molecular regulation of Lrrc10 expression, which probably also contributes to its tissue-specific expression.

Association of GSTP1 and RRM1 Polymorphisms with the Response and Toxicity of Gemcitabine-cisplatin Combination Chemotherapy in Chinese Patients with Non-small Cell Lung Cancer

  • Yuan, Zhi-Jun;Zhou, Wen-Wu;Liu, Wei;Wu, Bai-Ping;Zhao, Jin;Wu, Wei;He, Yi;Yang, Shuo;Su, Jing;Luo, Yi
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.10
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    • pp.4347-4351
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    • 2015
  • Background: Previous studies showed that genetic polymorphisms of glutathione S-transferase P1 (GSTP1) were involved in glutathione metabolism and genetic polymorphisms of ribonucleotide reductase (RRM1) were correlated with DNA synthesis. Here we explored the effects of these polymorphisms on the chemosensitivity and clinical outcome in Chinese non-small cell lung cancer (NSCLC) patients treated with gemcitabine-cisplatin regimens. Materials and Methods: DNA sequencing was used to evaluate genetic polymorphisms of GSTP1 Ile105Val and RRM1 C37A-T524C in 47 NSCLC patients treated with gemcitabine-cisplatin regimens. Clinical response was evaluated according to RECIST criteria after 2 cycles of chemotherapy and toxicity was assessed by 1979 WHO criteria (acute and subacute toxicity graduation criteria in chemotherapeutic agents). Results: There was no statistical significance between sensitive and non-sensitive groups regarding the genotype frequency distribution of GSTP1 Ile105Val polymorphism (p>0.05). But for RRM1 C37A-T524C genotype, sensitive group had higher proportion of high effective genotype than non-sensitive group (p=0.009). And according to the joint detection of GSTP1 Ile105Val and RRM1 C37A-T524C polymorphisms, the proportion of type A (A/A + high effective genotype) was significantly higher in sensitive group than in non-sensitive group (p=0.009). Toxicity showed no correlation with the genotypes between two groups (p>0.05). Conclusions: Compared with single detection of genetic polymorphisms of GSTP1 Ile105Val or RRM1 C37A-T524C, joint detection of both may be more helpful for patients with NSCLC to receive gemcitabine-cisplatin regimens as the first-line chemotherapy. Especially, genetic polymorphism of RRM1 is more likely to be used as an important biomarker to predict the response and toxicity of gemcitabine-cisplatin combination chemotherapy in NSCLC.

PPNC: Privacy Preserving Scheme for Random Linear Network Coding in Smart Grid

  • He, Shiming;Zeng, Weini;Xie, Kun;Yang, Hongming;Lai, Mingyong;Su, Xin
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.3
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    • pp.1510-1532
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    • 2017
  • In smart grid, privacy implications to individuals and their families are an important issue because of the fine-grained usage data collection. Wireless communications are utilized by many utility companies to obtain information. Network coding is exploited in smart grids, to enhance network performance in terms of throughput, delay, robustness, and energy consumption. However, random linear network coding introduces a new challenge for privacy preserving due to the encoding of data and updating of coefficients in forwarder nodes. We propose a distributed privacy preserving scheme for random linear network coding in smart grid that considers the converged flows character of the smart grid and exploits a homomorphic encryption function to decrease the complexities in the forwarder node. It offers a data confidentiality privacy preserving feature, which can efficiently thwart traffic analysis. The data of the packet is encrypted and the tag of the packet is encrypted by a homomorphic encryption function. The forwarder node random linearly codes the encrypted data and directly processes the cryptotext tags based on the homomorphism feature. Extensive security analysis and performance evaluations demonstrate the validity and efficiency of the proposed scheme.

Peptidoglycans Promotes Human Leukemic THP-1 Cell Apoptosis and Differentiation

  • Wang, Di;Xiao, Pei-Ling;Duan, Hua-Xin;Zhou, Ming;Liu, Jin;Li, Wei;Luo, Ke-Lin;Chen, Jian-Jun;Hu, Jin-Yue
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.12
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    • pp.6409-6413
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    • 2012
  • The innate immune system coordinates the inflammatory response to pathogens. To do so, its cells must discriminate self from non-self utilizing receptors that identify molecules synthesized exclusively by microbes. Toll-like receptors have a crucial role in the detection of microbial infection in mammals and insects. In mammals, they have evolved to recognize conserved products unique to microbial metabolism. These include lipopolysaccharide (LPS), lipotechoic acids, and peptidoglycans (PGN). We show here that TLRs, including TLR2, are expressed on the THP-1 human leukemia cell line. Activation of TLR2 signaling in THP-1 by PGN induces the synthesis of various soluble factors and proteins including interleukin-$1{\beta}$, interleukin-8 and TNF-${\alpha}$ and apoptosis of THP-1 with PGN dose and time dependence. Moreover, in this study we show that PGN induces apoptosis of THP-1 cells in a TNF-${\alpha}$-dependent manner. These findings indicate that TLR2 signaling results in a cascade leading to tumor apoptosis and differentiation, which may suggest new clinical prospects using TLR2 agonists as cytotoxic agents in certain cancers.