• 제목, 요약, 키워드: Hp

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전구체 프로합토글로빈과 성숙 합토글로빈의 시알산 함량 및 헤모글로빈-결합력 비교 (Differential Sialic Acid Content and Hemoglobin-binding Activity of Precursor Prohaptoglobin and Mature Haptoglobin)

  • 이주현;오미경;김인숙
    • 생명과학회지
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    • v.27 no.6
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    • pp.632-639
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    • 2017
  • 성숙 합토글로빈(haptoglobin, Hp)은 혈장 당단백질로서 혈중의 유리 헤모글로빈(hemoglobin, Hb)을 제거하고 항산화 작용을 한다. 프로합토글로빈(prohaptoglobin, proHp)은 Hp 전구체이며 혈중에 낮은 농도로 존재하지만 그 생리적 기능은 아직 확실하지 않다. 본 연구에서는 proHp와 Hp의 구조적, 기능적 차이를 연구하기 위하여 재조합 proHp를 제조하여 시알산과 Hb-결합력을 조사하고 성숙 Hp와 비교하였다. 비환원 조건의 Western blot 분석에서 proHp1은 약 130 kD의 하나의 밴드를 보였고 proHp2는 200 kDa 이상의 다중 밴드를 보였는데 이것은 대응되는 성숙 Hp1-1과 Hp2-2와 각각 동일한 양상이었다. 하지만 비환원 및 비변성 조건 하에서 수행된 전기영동에서는 proHp가 Hp보다 더 느리게 이동하였다. 렉틴을 이용한 ELISA 분석에서 proHp는 Hp보다 산성 당인 시 알산 함량이 더 낮음을 알 수 있었다. 또한 proHp는 Hb과의 결합력도 더 낮았다. 이러한 결과들로부터 proHp는 Hp와 같은 양상으로 중합체를 형성하지만 시알산 함량과 Hb-결합력이 Hp와 다름을 알 수 있었고 혈중에서 전구체인 proHp는 Hp와 다른 기능을 수행할 것임을 시사한다.

비즈니스 인사이드 - 한국HP, 세계 최초 울트라북 워크스테이션 출시

  • 대한인쇄문화협회
    • 프린팅코리아
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    • v.12 no.11
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    • pp.90-91
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    • 2013
  • 한국HP(대표 함기호, www.hp.co.kr)는 세계 최초 울트라북 워크스테이션인 HP ZBook 14와 업계 최초로 고속 데이터 전송 기술인 인텔 썬더볼트 및 최신 기능으로 무장한 워크스테이션 및 디스플레이를 포함한 Z시리즈 제품군을 공개했다. 이번에 선보인 Z시리즈는 매년 진행되는 HP 워크스테이션 솔루션 월드(HP Workstation Solution World)를 통해 공개됐으며, HP ZBook 모바일 워크스테이션 3종, 프로세서와 그래픽 성능이 향상된 HP Z데스크탑 워크스테이션 3종, 그리고 HP Z디스플레이(HP Z Displays) 신제품 2종이 포함됐다.

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조사료 생산을 위한 농업기계의 적정모형 설정 (Establishment of Optimum Pattern of Farm Machinery for Forage Production)

  • 김건엽;김정갑;한민수
    • 한국초지조사료학회지
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    • v.15 no.3
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    • pp.222-230
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    • 1995
  • This study was canied out to ddermine optimum areas for various sizes of land coverage of the farm machinery utilization in 1993-1994. A kind of machinery size and work systems were classed as the power tiller of 10HP+man power, the tractor of 35~46HP (tractor of 64~86HP and attachment were leased to harvest work), 64-86HP+ attachment and 90- 105HP+ attachment, respectively. \ulcornerhe results are summarized as follows: 1. The optimum areas of tractors of 90~105HP, 64~86HP and the power tiller of lOHP were estimated as 21.9 (corn-rye cropping system)- 26.9ha (sorghum $\times$ sudangrass - rye cropping system), 14.7 - 22.8ha and 1.2 - 1.61ha, respectively. The break-even-point areas of the tractors of 90-105HP. 64-86HP and the power tiller of lOHP were 16.6 (corn-rye cropping system)- 19.9ha (sorghum $\times$ sudangrass - rye cropping system), 12.5 - 16.lha and 0.12-0.13ha, respectively. 2. The optimum areas (land sizes, annual field capacity) for 50 cows by feeding rate(%) of roughage to concentrate were 6.8ha, 13.6ha in the 4060, 8.5ha, 17.0ha in the 5050 and 10.2ha, 20.4ha in the 60:40, and in case of 30 cows, it were 4.lha, 8.2ha in the 40:60, respectively. In the former case for the form of work system was the trador of 90-105HP+attachment and 64~86HP+ attachment, and the latter was the tractor of 35~46HP (tractor of 64~86HP and attachment were leased to harvest work) and 64-86HP+ attachment. 3. Productiori cost for corn-rye cropping system reducted to 51.8% in 102.9 wonkg dry matter the tractor of 90~ 105HP+ attachment with 213.4 wonkg dry matter the power tiller of 10HP+ man power.

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적분형 르장드르 형상함수를 이용한 단일 수준 적응적 hp-체눈 세분화 (Single Level Adaptive hp-Refinement using Integrals of Legendre Shape Function)

  • 조준형;유효진;우광성
    • 한국전산구조공학회논문집
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    • v.23 no.3
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    • pp.331-340
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    • 2010
  • 적응적 hp-세분화 기법과 그 기법의 효과적인 구성방법을 포함한 새로운 적응적 유한요소 알고리즘의 기초이론 및 적용이 이 연구를 통해 제시되었다. 적응적 hp-세분화 기초의 유한요소기법은 적분형 르장드르 형상함수와 요소별로 불균등한차수의 분배 및 비정형적인 절점연결과 관련된 연속조건을 만족시킬 수 있는 제약조건을 필요로 한다. 따라서 요소간의 접합부분에서 적응적 hp-유한요소망의 연속성이 중요한 문제로 대두된다. 이러한 문제를 요소경계에 연속성 제약조건을 절점연결 사상행렬을 적용하여 해결하였다. 또한, 적분형 르장드르 형상함수의 계층성질을 이용하여 제시된 알고리즘의 효율적 정식화 방안을 제시하였다. 간단한 캔틸레버문제가 h-세분화, p-세분화 그리고 hp-세분화 방법에 의해 계산되었다. hp-세분화의 결과는 다른 방식의 세분화에 비해 보다 빠른 수렴성을 보여 주는 것이 확인되었다. 그러므로 제시된 hp-세분화 알고리즘은 실제문제에 효율적으로 적용될 수 있을 것으로 생각된다.

Isoelectric Focusing of the Hemolymph Proteins of the Silkworm, Bombyx mori

  • Staykova, Teodora;Popov, Petar;Grekov, Dimitar;Terzieva, Petia
    • International Journal of Industrial Entomology
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    • v.8 no.1
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    • pp.117-121
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    • 2004
  • Soluble proteins of silkworm hemolymph were investigated by means of isoelectric focusing (IEF). The protein spectra during ontogenesis of races and inter-races hybrids kept in Bulgaria was studied. A total of 51 protein bands in the hemolymph from fourth larval instar to imago were ascertained. Stage specific expression was established. The specific expression of some protein bands in the individual spectra manifest phenotype of gene determinate polymorphism (HP F, HP J, HP K, HP L, HP Q - in the zone with pH gradient 3.5-6.2 and HP K, HP L, HP N, HP P, HP T - in the zone with pH gradient 9.5 - 6.2). Breed specific expression was observed. On the basis of the obtained results, it was established that the investigated breeds are heterogeneous and the isoelectric focusing method is successful when specifying the inner-race and inter-race polymorphism in silkworm.

Estimating coefficient of consolidation and hydraulic conductivity from piezocone test results - Case studies

  • Hossain, Md. Julfikar;Chai, Jinchun
    • Geomechanics and Engineering
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    • v.6 no.6
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    • pp.577-592
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    • 2014
  • The methods for estimating in-situ hydraulic conductivity ($k_{hp}$) and coefficient of consolidation ($c_{hp}$) in the horizontal direction from piezocone penetration and dissipation test results have been investigated using test results at two sites in Saga, Japan. At the two sites the laboratory values of hydraulic conductivity ($k_v$) and coefficient of consolidation ($c_v$) in the vertical direction are also available. Comparing $k_{hp}$ with $k_v$ and $c_{hp}$ with $k_v$ values, suitable methods for estimating $k_{hp}$ and $c_{hp}$ values are recommended. For the two sites, where $k_{hp}{\approx}k_v$ and $c_{hp}{\approx}2c_v$. It is suggested that the estimated values of $k_{hp}$ and $c_{hp}$ can be used in engineering design.

Protein Kinase C-delta Stimulates Haptoglobin Secretion

  • Oh, Mi-Kyung;Park, Seon-Joo;Kim, Nam-Hoon;Kim, In-Sook
    • BMB Reports
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    • v.40 no.1
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    • pp.130-134
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    • 2007
  • Haptoglobin (Hp) is a glycoprotein that is produced by hepatic cells and secreted into the circulation. While studying the physiologic functions of Hp, we found that Hp synthesized in THP-1 monocytic cells was largely retained within cells, although Hp is considered a secretory protein. To investigate the molecular mechanism on Hp secretion in THP-1 cells, in the present study, we examined the effect of protein kinase C (PKC) on Hp secretion. When several inhibitors of PKC isoforms were tested, only Rottlerin, a specific inhibitor of PKC-$\delta$, completely blocked Hp secretion from cells to culture medium. To confirm the role of PKC-$\delta$ in Hp secretion, Hp-overexpressing COS7 cells were transiently transfected with a wild-type or a dominantnegative mutant of the PKC-$\delta$ gene. Mutant PKC-$\delta$ significantly inhibited Hp secretion, whereas the wild-type gene slightly increased Hp secretion. These results demonstrate that the PKC-$\delta$ signal is involved in Hp secretion.

Endoprotease and Exopeptidase Activities in the Hepatopancreas of the Cuttlefish Sepia officinalis, the Squid Todarodes pacificus, and the Octopus Octopus vulgaris Cuvier

  • Kim, Min Ji;Kim, Hyeon Jeong;Kim, Ki Hyun;Heu, Min Soo;Kim, Jin-Soo
    • Fisheries and aquatic sciences
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    • v.15 no.3
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    • pp.197-202
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    • 2012
  • This study examined and compared the exopeptidase and endoprotease activities of the hepatopancreas (HP) of cuttlefish, squid, and octopus species. The protein concentration in crude extract (CE) from octopus HP was 3,940 mg/100 g, lower than those in CEs from squid HP (4,157 mg/100 g) and cuttlefish HP (5,940 mg/100 g). With azocasein of pH 6 as a substrate, the total activity in HP CE of octopus was 31,000 U, lower than the values for cuttlefish (57,000 U) and squid (69,000 U). Regardless of sample type, the total activities of the CEs with azocasein as the substrate were higher at pH 6 (31,000-69,000 U) than at pH 9 (19,000-34,000 U). With L-leucine-p-nitroanilide (LeuPNA) of pH 6 as the substrate, the total activity of the HP CE from octopus was 138,000 U, higher than values from both cuttlefish HP (72,000 U) and squid HP (63,000 U). Regardless of sample type, the total activities of the CEs with LeuPNA as the substrate were higher at pH 6 (63,000-138,000 U) than at pH 9 (41,000-122,000 U). With LeuPNA as the substrate, the total activities of the CEs from octopus HP and cuttlefish HP were higher at pH 6 than at pH 9. However, there was no difference in total activity between pH 6 and 9 for squid HP CE with LeuPNA as the substrate. These results suggest that the octopus HP is superior to the cuttlefish HP and squid HP as a potential resource for extracting exopeptidases. Exopeptidases from octopus HP have potential industrial applications and their use might aid in reducing pollution related to the octopus industry.

초임계 유체를 이용한 PLGA 입자 제조에 첨가제가 미치는 영향 (Effect of an Excipient on the Formation of PLGA Particles Using Supercritical Fluid)

  • 정인일;함승주;임교빈;유종훈
    • 폴리머
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    • v.36 no.1
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    • pp.1-8
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    • 2012
  • 본 연구에서는 초임계 aerosol solvent extraction system(ASES) 공정을 이용하여 poly(lactic-$co$-glycolic acid)(PLGA) 미립자를 제조하기 위해 첨가제로 hydroxypropyl-${\beta}$-cyclodextrin (HP-${\beta}$-CD)를 사용하였으며, HP-${\beta}$-CD의 첨가량이 PLGA 입자의 형상에 미치는 영향을 조사하였다. 또한 항암제인 파클리탁셀이 봉입된 미립자를 제조하여 HP-${\beta}$-CD의 함량에 따른 약물 방출 특성의 변화를 고찰하였다. PLGA(75:25)의 경우 HP-${\beta}$-CD를 고분자 중량 대비 약 40%까지, PLGA(50:50)의 경우 약 30%까지 첨가하였을 때 미립자가 형성되었으며, HP-${\beta}$-CD의 첨가량이 이보다 더 적은 경우에는 PLGA가 필름 형태로 형성되었다. 파클리탁셀이 봉입된 PLGA/HP-${\beta}$-CD 미립자의 경우 HP-${\beta}$-CD의 함량이 증가함에 따라 더 빠른 속도로 약물이 방출됨을 확인할 수 있었다.