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Differential Sialic Acid Content and Hemoglobin-binding Activity of Precursor Prohaptoglobin and Mature Haptoglobin (전구체 프로합토글로빈과 성숙 합토글로빈의 시알산 함량 및 헤모글로빈-결합력 비교)

  • Lee, Joo-Hyun;Oh, Mi-Kyung;Kim, In-Sook
    • Journal of Life Science
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    • v.27 no.6
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    • pp.632-639
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    • 2017
  • Mature haptoglobin (Hp) is a plasma glycoprotein and acts as an antioxidant by scavenging cell-free hemoglobin (Hb). Prohaptoglobin (proHp) is an unprocessed Hp precursor which is present a little in circulation. However, the biological function of proHp remains unknown. To investigate the structural and functional differences between proHp and Hp, we prepared recombinant proHp isoforms and compared their sialic acid content and Hb-binding capacity with those of mature isoforms. When proHp samples were analyzed by Western blot under non-reducing conditions, proHp1 was detected as one band of approximately 130 kDa and proHp2 as multiple bands >200 kDa, in the manner of mature Hp1-1 and Hp2-2, respectively. On the native polyacrylamide gel under non-reducing and non-denaturing conditions, both proHp isoforms migrated more slowly than their mature Hp counterparts. In addition, the lectin-based ELISA assay demonstrated that the content of sialic acid in proHp1 and proHp2 was much less than in Hp1-1 and Hp2-2. The Hb-binding capacity of proHp was also lower than those of mature Hp. These findings indicate that proHp and Hp are similar in the size and polymerization pattern, but different in sialic acid content and Hb-binding activity. It suggests precursor proHp may exert different functions in circulation than does mature Hp.

비즈니스 인사이드 - 한국HP, 세계 최초 울트라북 워크스테이션 출시

  • 대한인쇄문화협회
    • 프린팅코리아
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    • v.12 no.11
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    • pp.90-91
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    • 2013
  • 한국HP(대표 함기호, www.hp.co.kr)는 세계 최초 울트라북 워크스테이션인 HP ZBook 14와 업계 최초로 고속 데이터 전송 기술인 인텔 썬더볼트 및 최신 기능으로 무장한 워크스테이션 및 디스플레이를 포함한 Z시리즈 제품군을 공개했다. 이번에 선보인 Z시리즈는 매년 진행되는 HP 워크스테이션 솔루션 월드(HP Workstation Solution World)를 통해 공개됐으며, HP ZBook 모바일 워크스테이션 3종, 프로세서와 그래픽 성능이 향상된 HP Z데스크탑 워크스테이션 3종, 그리고 HP Z디스플레이(HP Z Displays) 신제품 2종이 포함됐다.

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Establishment of Optimum Pattern of Farm Machinery for Forage Production (조사료 생산을 위한 농업기계의 적정모형 설정)

  • 김건엽;김정갑;한민수
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.15 no.3
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    • pp.222-230
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    • 1995
  • This study was canied out to ddermine optimum areas for various sizes of land coverage of the farm machinery utilization in 1993-1994. A kind of machinery size and work systems were classed as the power tiller of 10HP+man power, the tractor of 35~46HP (tractor of 64~86HP and attachment were leased to harvest work), 64-86HP+ attachment and 90- 105HP+ attachment, respectively. \ulcornerhe results are summarized as follows: 1. The optimum areas of tractors of 90~105HP, 64~86HP and the power tiller of lOHP were estimated as 21.9 (corn-rye cropping system)- 26.9ha (sorghum $\times$ sudangrass - rye cropping system), 14.7 - 22.8ha and 1.2 - 1.61ha, respectively. The break-even-point areas of the tractors of 90-105HP. 64-86HP and the power tiller of lOHP were 16.6 (corn-rye cropping system)- 19.9ha (sorghum $\times$ sudangrass - rye cropping system), 12.5 - 16.lha and 0.12-0.13ha, respectively. 2. The optimum areas (land sizes, annual field capacity) for 50 cows by feeding rate(%) of roughage to concentrate were 6.8ha, 13.6ha in the 4060, 8.5ha, 17.0ha in the 5050 and 10.2ha, 20.4ha in the 60:40, and in case of 30 cows, it were 4.lha, 8.2ha in the 40:60, respectively. In the former case for the form of work system was the trador of 90-105HP+attachment and 64~86HP+ attachment, and the latter was the tractor of 35~46HP (tractor of 64~86HP and attachment were leased to harvest work) and 64-86HP+ attachment. 3. Productiori cost for corn-rye cropping system reducted to 51.8% in 102.9 wonkg dry matter the tractor of 90~ 105HP+ attachment with 213.4 wonkg dry matter the power tiller of 10HP+ man power.

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Single Level Adaptive hp-Refinement using Integrals of Legendre Shape Function (적분형 르장드르 형상함수를 이용한 단일 수준 적응적 hp-체눈 세분화)

  • Jo, Jun-Hyung;Yoo, Hyo-Jin;Woo, Kwang-Sung
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.23 no.3
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    • pp.331-340
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    • 2010
  • The basic theory and application of new adaptive finite element algorithm have been proposed in this study including the adaptive hp-refinement strategy, and the effective method for constructing hp-approximation. The hp-adaptive finite element concept needs the integrals of Legendre shape function, nonuniform p-distribution, and suitable constraint of continuity in conjunction with irregular node connection. The continuity of hp-adaptive mesh is an important problem at the common boundary of element interface. To solve this problem, the constraint of continuity has been enforced at the common boundary using the connectivity mapping matrix. The effective method for constructing of the proposed algorithm has been developed by using hierarchical nature of the integrals of Legendre shape function. To verify the proposed algorithm, the problem of simple cantilever beam has been solved by the conventional h-refinement and p-refinement as well as the proposed hp-refinement. The result obtained by hp-refinement approach shows more rapid convergence rate than those by h-refinement and p-refinement schemes. It it noted that the proposed algorithm may be implemented efficiently in practice.

Isoelectric Focusing of the Hemolymph Proteins of the Silkworm, Bombyx mori

  • Staykova, Teodora;Popov, Petar;Grekov, Dimitar;Terzieva, Petia
    • International Journal of Industrial Entomology
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    • v.8 no.1
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    • pp.117-121
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    • 2004
  • Soluble proteins of silkworm hemolymph were investigated by means of isoelectric focusing (IEF). The protein spectra during ontogenesis of races and inter-races hybrids kept in Bulgaria was studied. A total of 51 protein bands in the hemolymph from fourth larval instar to imago were ascertained. Stage specific expression was established. The specific expression of some protein bands in the individual spectra manifest phenotype of gene determinate polymorphism (HP F, HP J, HP K, HP L, HP Q - in the zone with pH gradient 3.5-6.2 and HP K, HP L, HP N, HP P, HP T - in the zone with pH gradient 9.5 - 6.2). Breed specific expression was observed. On the basis of the obtained results, it was established that the investigated breeds are heterogeneous and the isoelectric focusing method is successful when specifying the inner-race and inter-race polymorphism in silkworm.

Estimating coefficient of consolidation and hydraulic conductivity from piezocone test results - Case studies

  • Hossain, Md. Julfikar;Chai, Jinchun
    • Geomechanics and Engineering
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    • v.6 no.6
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    • pp.577-592
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    • 2014
  • The methods for estimating in-situ hydraulic conductivity ($k_{hp}$) and coefficient of consolidation ($c_{hp}$) in the horizontal direction from piezocone penetration and dissipation test results have been investigated using test results at two sites in Saga, Japan. At the two sites the laboratory values of hydraulic conductivity ($k_v$) and coefficient of consolidation ($c_v$) in the vertical direction are also available. Comparing $k_{hp}$ with $k_v$ and $c_{hp}$ with $k_v$ values, suitable methods for estimating $k_{hp}$ and $c_{hp}$ values are recommended. For the two sites, where $k_{hp}{\approx}k_v$ and $c_{hp}{\approx}2c_v$. It is suggested that the estimated values of $k_{hp}$ and $c_{hp}$ can be used in engineering design.

Protein Kinase C-delta Stimulates Haptoglobin Secretion

  • Oh, Mi-Kyung;Park, Seon-Joo;Kim, Nam-Hoon;Kim, In-Sook
    • BMB Reports
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    • v.40 no.1
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    • pp.130-134
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    • 2007
  • Haptoglobin (Hp) is a glycoprotein that is produced by hepatic cells and secreted into the circulation. While studying the physiologic functions of Hp, we found that Hp synthesized in THP-1 monocytic cells was largely retained within cells, although Hp is considered a secretory protein. To investigate the molecular mechanism on Hp secretion in THP-1 cells, in the present study, we examined the effect of protein kinase C (PKC) on Hp secretion. When several inhibitors of PKC isoforms were tested, only Rottlerin, a specific inhibitor of PKC-$\delta$, completely blocked Hp secretion from cells to culture medium. To confirm the role of PKC-$\delta$ in Hp secretion, Hp-overexpressing COS7 cells were transiently transfected with a wild-type or a dominantnegative mutant of the PKC-$\delta$ gene. Mutant PKC-$\delta$ significantly inhibited Hp secretion, whereas the wild-type gene slightly increased Hp secretion. These results demonstrate that the PKC-$\delta$ signal is involved in Hp secretion.

Endoprotease and Exopeptidase Activities in the Hepatopancreas of the Cuttlefish Sepia officinalis, the Squid Todarodes pacificus, and the Octopus Octopus vulgaris Cuvier

  • Kim, Min Ji;Kim, Hyeon Jeong;Kim, Ki Hyun;Heu, Min Soo;Kim, Jin-Soo
    • Fisheries and aquatic sciences
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    • v.15 no.3
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    • pp.197-202
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    • 2012
  • This study examined and compared the exopeptidase and endoprotease activities of the hepatopancreas (HP) of cuttlefish, squid, and octopus species. The protein concentration in crude extract (CE) from octopus HP was 3,940 mg/100 g, lower than those in CEs from squid HP (4,157 mg/100 g) and cuttlefish HP (5,940 mg/100 g). With azocasein of pH 6 as a substrate, the total activity in HP CE of octopus was 31,000 U, lower than the values for cuttlefish (57,000 U) and squid (69,000 U). Regardless of sample type, the total activities of the CEs with azocasein as the substrate were higher at pH 6 (31,000-69,000 U) than at pH 9 (19,000-34,000 U). With L-leucine-p-nitroanilide (LeuPNA) of pH 6 as the substrate, the total activity of the HP CE from octopus was 138,000 U, higher than values from both cuttlefish HP (72,000 U) and squid HP (63,000 U). Regardless of sample type, the total activities of the CEs with LeuPNA as the substrate were higher at pH 6 (63,000-138,000 U) than at pH 9 (41,000-122,000 U). With LeuPNA as the substrate, the total activities of the CEs from octopus HP and cuttlefish HP were higher at pH 6 than at pH 9. However, there was no difference in total activity between pH 6 and 9 for squid HP CE with LeuPNA as the substrate. These results suggest that the octopus HP is superior to the cuttlefish HP and squid HP as a potential resource for extracting exopeptidases. Exopeptidases from octopus HP have potential industrial applications and their use might aid in reducing pollution related to the octopus industry.

Effect of an Excipient on the Formation of PLGA Particles Using Supercritical Fluid (초임계 유체를 이용한 PLGA 입자 제조에 첨가제가 미치는 영향)

  • Jung, In-Il;Haam, Seung-Joo;Lim, Gio-Bin;Ryu, Jong-Hoon
    • Polymer Korea
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    • v.36 no.1
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    • pp.1-8
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    • 2012
  • In this study, we employed hydroxypropyl-${\beta}$-cyclodextrin (HP-${\beta}$-CD) as an excipient to produce poly(lactic-$co$-glycolic acid) (PLGA) fine particles by a supercritical fluid process, called aerosol solvent extraction system (ASES), and investigated the effect of HP-${\beta}$-CD content on the morphology of the particles. The influence of HP-${\beta}$-CD on the drug release characteristics of paclitaxel-loaded PLGA particles was also evaluated. Fine particles were obtained when the HP-${\beta}$-CD content in PLGA/HP-${\beta}$-CD mixtures was greater than 40% and 30%, respectively, for PLGA(75:25) and PLGA(50:50), whereas a film-like precipitate was obtained for lower HP-${\beta}$-CD content. The release rate for paclitaxel loaded PLGA(75:25)/HP-${\beta}$-CD particles was found to increase with HP-${\beta}$-CD content.