• Title, Summary, Keyword: Host range

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Host Range of pTi12 Contained Agrobacterium tumefaciens KU12 Isolated from Korea (pTi-12를 함유한 한국산 Agrobacterium tumefaciens KU12의 숙주범위)

  • 전경아
    • Journal of Plant Biology
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    • v.33 no.2
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    • pp.97-104
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    • 1990
  • In order to investigate the host range of Agrobacterium tumefaciens KU12 containing pTi-12, 28 species of dicotyledonous plants were infected with KU12, A136 without Ti plasmid and A348 containing pTi A6, respectively. KU12 and A348 induced tumor in 20 species and 14 species, respectively. This results showed that KU12 has a wide host range. Therefore, it was confirmed that KU12 and pTi-12 are very useful for developing plant vector system having a broad host range.

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Vegetative Compatibility Grouping and Pathogenicity of Colletotrichum gloeosporioides Isolates from Different Host Plants

  • Ahn, Il-Pyung;Kim, Soonok;Im, Kyung-Hwan;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.19 no.6
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    • pp.269-273
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    • 2003
  • A total of 57 isolates of Colletotrichum gloeosporioides were recovered from diseased tissues of Hall's crab apple (Malus haliana), 3 cultivars of edible apple (M. pumila var. dulcissima), red pepper (Capsicum annum), and grapevine (Vitis vinifera) fruits. All isolates showed strong virulence on their own host plants. Isolates from edible apple exhibited high level of cultivar specificity in pathogenicity tests. Ten isolates from apple cultivar 'Fuji' were virulent on 'Jonathan' and 'Rall's Genet'. However, 12 isolates from 'Jonathan' and 'Rall's Genet' were not virulent on 'Fuji'. Among the 24 isolates from red pepper, only seven and two isolates were infective on edible apple and grapevine fruits, respectively. All six isolates from grapevine were only virulent on their own host. These isolates were grouped into five vegetative compatibility groups (VCGs), A, B, C, D, and E, by demonstrating heterokaryosis through complementation using nitrate-nonutilizing (nit) mutants. Among them, isolates belong to VCG-A and VCG-D accounted for 24 and 17 isolates; those in VCG-A exhibited wide host range involving Hall's crab apple, all three edible apple cultivars, and red pepper. On the other hand, isolates of VCG-D and VCG-E showed limited host range specific to red pepper and grapevine, respectively. Taken together, the data suggest that among C. gloeosporioides isolates, the concepts of pathotype and/or forma specialis may exist, and that three is a relationship between host specificity and VCG grouping among C. gloeosporioides isolates.

Effective Combination of Agrobacterium tumefaciens Strains and Ti Plasmids for the Construction of Plant Vector System

  • Kim, Mi-Suk;Park, Jeong-Du;Eum, Jin-Seong;Sim, Woong-Seop
    • Journal of Plant Biology
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    • v.39 no.3
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    • pp.179-184
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    • 1996
  • The purpose of this study is to obtain the most efficient combination of Agrobacterium tumefaciens strains and Ti plasmids for the construction of dicotyledonous plant vector system. Ti plasmid-curing A. tumefaciens A136 and KU12C3 were transformed with four kinds of Ti plasmids, pTiBo542, pTiA6, pTiKU12 and pTiAch5, respectively. The stems of 28 species of dicotyledonous plants were then inoculated with these transformants and examined for crown gall formation. The different combination of A. tumefaciens strains and Ti plasmids showed quite a difference in terms of the crown gall formation. Agrobacterium strins A136 and KU12C3 have a same plant host range in case that both strains harour the same kind of Ti plasmid, pTiBo542 or pTiAch5. However, the above-mentioned both strains have quite different host range in the event of containing the same Ti plasmid, pTiKU12 or pTiA6. In case that KU12C3 contains pTiA6 or pTiKU12, this strain has a wider plant host range than A136. The plant host range of pTiBo542 is the widest, followed by pTiA6, pTiKU12 and pTiAch5. Twelve plants among 28 tested plants are not transformed by any virulent Agrobacterium strains used in this study. In conclusion, A. tumefaciens KU12C3 and A136 harboring pTiBo542 showed the widest host range for transforming dicotyledonous plants. Also, it was acertained that the host range of Ti plasmids is affected by chromosomal level.

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Occurrence and Host Plant of Metcalfa Pruinosa (Say) (Hemiptera: Flatidae) in Korea (국내 미국선녀벌레의 분포 및 기주식물)

  • Kim, Dong-Eon;Kil, Jihyon
    • Journal of Environmental Science International
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    • v.23 no.8
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    • pp.1385-1394
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    • 2014
  • Metcalfa pruinosa was considered to be a very harmful invasive species, due to its high species density in nature and wide range of its host plants. M. pruinosa was distributed in 28 sites among 143 sites. M. pruinosa has wide range and diverse host plants of 52 families 110 species including crop, fruits and forest trees. At present, the identified host plant of M. pruinosa are composed of 62 families and 145 species in total since their first appearance was reported. M. pruinosa was found in roadside 46.7%, followed by forests 33.3% and orchard 20%. Nymphs and adults cause damage to crop and orchard by sucking juice, outbreak of fungi through secretion of wax, and reduction of plant assimilation due to the nectar of nymphs. Also, it reduces the merchantable quality of fruits and thus causes economic damage. It is judged that M. pruinosa has been moved along major road via the traffic vehicles.

Molecular Biological Characterization of Recombinant Baculovirus with an Expanded Host Range (숙주범위가 넓어진 유전자 재조합 핵다각체병 바이러스의 분자생물학적 특성)

  • 김우진;우수동
    • Journal of Sericultural and Entomological Science
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    • v.38 no.1
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    • pp.42-47
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    • 1996
  • To investigate the host range determining factors of nuclear polyhedrois virus (NPV), Autographa california NPV and Bombyx mori NPV were coinfected into the two different cell lines, BmN-4 and Sf-9. The recombinant baculoviruses, RecS-A6 and RecB-727 which have an expanded host range, were isolated from Sf-9 and BmN-4 cell lines, respectively. The molecular biological characteristics of the recombinant baculoviruses were investigated. The pathogenicity of RecB-727 was similar to that of wild type BmNPV, while the pathogenicity of RecS-A6 was relatively lower than that of wild type BmNPV. The restriction enzyme digestion patterns of parental viruses and recombinant viruses showed that the recombinant virus has an expanded host range by genetic recombination. Southern blot analysis revealed that the p10 gene of RecB-727 was derived from AcNPV genomic DNA, while RecS-A6 has p10 gene of BmNPV in a viral genome. To investigate the host range expansion mechanism of recombinant baculovirus, HindIII-SacI 0.6 kb DNA fragments of RecS-A6 and RecB-727 were cloned and sequenced. The results showed that of wild type BmNPV helicase gene, suggesting that the expanded host range of recombinant baculoviruses was due to the insertion of BmNPV helicase gene into AcNPV viral genome.

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Virulence Factors and Stability of Coliphages Specific to Escherichia coli O157:H7 and to Various E. coli Infection

  • Kim, Eun-Jin;Chang, Hyun-Joo;Kwak, Soojin;Park, Jong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.26 no.12
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    • pp.2060-2065
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    • 2016
  • Characteristics of E. coli O157:H7-specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for other E. coli serotypes (broad-host coliphages) were compared. The burst sizes of the two groups ranged from 40 to 176 PFU/infected cell. Distributions of the virulence factors stx1, stx2, ehxA, and saa between the two groups were not differentiated. Broad-host-range coliphages showed lower stability at $70^{\circ}C$, in relation to O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerance by reduction of only 22% and 11% phages, respectively, under pH 3 and 70% ethanol for 1 h exposure. Therefore, these results revealed that the O157 coliphages might be more stable under harsh environments, which might explain their effective infection of the acid-tolerant E. coli O157:H7.

Diversity of Bacteriophages Infecting Xanthomonas oryzae pv. oryzae in Paddy Fields and Its Potential to Control Bacterial Leaf Blight of Rice

  • Chae, Jong-Chan;Nguyen, Bao Hung;Yu, Sang-Mi;Lee, Ha Kyung;Lee, Yong Hoon
    • Journal of Microbiology and Biotechnology
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    • v.24 no.6
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    • pp.740-747
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    • 2014
  • Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a very serious disease in rice-growing regions of the world. In spite of their economic importance, there are no effective ways of protecting rice plants from this disease. Bacteriophages infecting Xoo affect the population dynamics of the pathogen and consequently the occurrence of the disease. In this study, we investigated the diversity, host range, and infectivity of Xoo phages, and their use as a bicontrol agent on BLB was tested. Among the 34 phages that were isolated from floodwater in paddy fields, 29 belonged to the Myoviridae family, which suggests that the dominant phage in the ecosystem was Myoviridae. The isolated phages were classified into two groups based on plaque size produced on the lawn of Xoo. In general, there was a negative relationship between plaque size and host range, and interestingly the phages having a narrow host range had low efficiency of infectivity. The deduced protein sequence analysis of htf genes indicated that the gene was not a determinant of host specificity. Although the difference in host range and infectivity depending on morphotype needs to be addressed, the results revealed deeper understanding of the interaction between the phages and Xoo strains in floodwater and damp soil environments. The phage mixtures reduced the occurrence of BLB when they were treated with skim milk. The results indicate that the Xoo phages could be used as an alternative control method to increase the control efficacy and reduce the use of agrochemicals.

Studies on the Host Range of Rice Stripe Virus (벼 줄무의잎마름병 바이러스의 기주범위에 관한 연구)

  • Chung Bong Cho;Lee Soon Hyung
    • Korean journal of applied entomology
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    • v.10 no.2
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    • pp.85-89
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    • 1971
  • An investigation was carried out to know the host range of the rice stripe virus as a basis of the disease control. 1. In order to investigate the host range of the rice stripe virus 30 species including gramineaceae and forage crop and weeds were inoculated with viruliferous Laodelphax sriatellus Fallen. As a result, 21 out of 30 species showed typical symptoms. 2. Cyperus amuricus Maximowicz var. laxus., Pycreus sanguinolentus Nees. and Eriocaulon robustius Makino, not belonging to gramineaceae were known to be new host plants of rice stripe virus. The highest infection occurred on Pycreus sanguinolentus grown widly in the wet paddy fold. 3. The number of vectors inoculated on the plants was decreased after 48 hours than that of 24 hours.

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A Performance Comparison Routing Protocols for control Mobile Host (Mobile Host 제어를 위한 Routing Protocol 성능 비교)

  • 김탁근;이광재;김동일
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • pp.129-132
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    • 2001
  • The ad hoc network was composed of Mobile hosts. Due to the limited transmission range of each mobile node hosts, in such an environment, it is essential for other hosts to coorperate to transfer packet to destination. Moreover, the existing routing protocol algorithms are not efficient on the ad hoc network because a mobile host operates router without fixed router. In this paper, we will compare and analysis for the performance of existing ad hoc network routing protocols through simulation.

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Characteristics and Pathogenicity of Host Range Expanded Recombinant Viruses in Insect Cells (숙주범위가 넓어진 재조합 바이러스의 세포주에서의 특성 및 병원성)

  • Kim, Hye-Sung;Woo, Soo-Dong;Kim, Woo-Jin;Choi, Jae-Young;Jin, Byung-Rae;Lee, Youn-Hyung;Kang, Seok-Kwon
    • The Journal of Korean Society of Virology
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    • v.27 no.1
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    • pp.29-37
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    • 1997
  • To use recombinant viruses with wider host range as viral insecticides, we investigated the characteristics and pathogenicity of host range expanded recombinant viruses in insect cells. We compared host range expanded recombinant viruses, RecS-B6 and RecB-8, constructed by cotransfection of Autographa californica nuclear polyhedrosis virus (AcNPV) and Bombyx mori NPV (BmNPV), to host range expanded AcNPV, Ac-BH, by substitution of the 0.6 Kb fragment of the BmNPV helicase gene. Restriction endonuclease profiles of RecS-B6 and RecB-8 DNAs were different from those of parent viruses. Nucleotide sequence analysis of the 0.6 Kb region in the putative helicase gene of RecS-B6 and RecB-8 showed that their structures were identical to the counterpart region of BmNPV. Comparison of viral replication of these recombinant viruses in Sf-21 and BmN-4 cells showed that Ac-BH, compared to wild type viruses, replicated well in BmN-4 cells but poorly in Sf-21 cells. In contrast, RecS-B6 and RecB-8 replicated relatively well in both cells compared to parent viruses. These results may imply that random genomic recombinant viruses, RecS-B6 and RecB-8, possess better potential as viral pesticides than helicase-mediated recombinant virus, Ac-BH.

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